scholarly journals Serum proteins and lipids in mild form of calf bronchopneumonia: candidates for reliable biomarkers

2017 ◽  
Vol 67 (2) ◽  
pp. 201-221 ◽  
Author(s):  
Marijana Kovačić ◽  
Dragana Marković ◽  
Irina Maslovarić ◽  
Sonja Obrenović ◽  
Jelica Grujić-Milanović ◽  
...  
Keyword(s):  
Acute Phase ◽  
Peripheral Blood ◽  
Pasteurella Multocida ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Hdl Cholesterol ◽  
Matrix Metalloproteases ◽  
Γ Globulins ◽  
Biochemical Analyses

Abstract Calf bronchopneumonia is complex multifactorial disease and for its accurate diagnosis and therapy, besides clinical examination, microbiologic, hematologic and biochemical analyses could be necessary. In general, additional analyses are not implemented, mainly because the disease biomarkers are not defined. To establish which analysis might be useful for determining the severity of the disease, we analyzed 23 three-month old calves with mild clinical signs of bronchopneumonia and 15 age-matched healthy calves. Pasteurella multocida was isolated from deep nasal swabs of diseased calves. Peripheral blood erythrocyte and leukocyte count of bronchopneumonic and healthy calves showed no difference. Serum proteins, lipoproteins and lipids were analyzed with spectrophotometry, agarose gel electrophoresis, non-reducing SDS-PAGE, gel zymography, and thin-layer chromatography. The bronchopneumonic calves had an increased level of circulating immune complexes and α globulins, which contain some of the positive acute phase proteins. In diseased calves the increased concentration of total γ globulins (IgG), due to an increased concentration of anionic γ globulins (predominately IgG1), was detected. The increased concentration of anionic γ globulins followed by increased concentration of transferrin (negative acute phase protein) and HDL cholesterol, decreased concentration of LDL-cholesterol, unchanged activity of matrix metalloproteases and leukocyte counts might reflect the obvious absence of generalized inflammation. A positive correlation was found between the acquired results and the appearance of mild clinical signs. Therefore, we believe that the parameters analyzed in the peripheral blood could be applied as reliable disease markers to distinguish between severe (inflammatory) and mild forms of calf bronchopneumonia and to predict a better outcome for these calves.

scholarly journals Serum concentrations of acute phase proteins and immunoglobulins of calves with rotavirus diarrhea

2016 ◽  
Vol 68 (4) ◽  
pp. 865-872 ◽  
Author(s):  
T.G. Rocha ◽  
F.D.F. Silva ◽  
C. Bortoletto ◽  
D.G. Silva ◽  
M.G. Buzinaro ◽  
...  
Keyword(s):  
Acute Phase ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Least Square ◽  
Serum Concentrations ◽  
Fecal Samples ◽  
Acid Glycoprotein ◽  
Group B ◽  
Rotavirus Diarrhea

ABSTRACT The aim of the present study was to characterize changes in acute phase protein levels according to the occurrence of rotavirus diarrhea in calves in the first month of life. Blood and fecal samples were taken before colostrum intake and at 1, 2, 7, 15, 21 and 30 days of age from 24 Holstein calves allotted in three experimental groups: calves that did not present diarrhea (group A), calves that presented diarrhea, but tested negative for rotavirus in feces (group B), and calves that presented diarrhea and tested positive for rotavirus in feces (group C) (experiment 1). When the animals presented episodes of diarrhea, blood and fecal samples were taken at 24-hour intervals until the end of clinical signs (experiment 2). Serum proteins were separated by SDS-PAGE technique and rotavirus in feces was detected by PAGE. Data of experiment 1 were analyzed by ANOVA and Tukey's test, considered significant at P<0.05. Data of experiment 2 were subjected to the HSD test. Total protein, globulins, and IgG concentrations were lower in group C than in groups A and B. Ceruloplasmin and transferrin levels were higher in group C than in groups A and B. Serum concentrations of haptoglobin and α1-acid glycoprotein did not differ significantly between groups throughout the experimental period. Calves presented diarrhea between 10.4 and 14.6 days of age in group B, and between 10.3 and 14.6 days of age in group C. In the moments of diarrhea manifestation, least square means of IgA, haptoglobin and α1-acid glycoprotein concentrations did not differ significantly between groups B and C, but ceruloplasmin and transferrin concentrations were higher in group C than in group B, as opposed to what occurred with IgG levels. These findings show that optimizing passive immunity transfer of immunoglobulins decrease the likelihood of calves developing diarrhea caused by rotavirus. In addition, ceruloplasmin presents characteristics of a biomarker of rotavirus infection in calves.

scholarly journals Serum protein concentrations, including acute phase proteins, in calves with hepatogenous photosensitization

2007 ◽  
Vol 59 (6) ◽  
pp. 1355-1358 ◽  
Author(s):  
J.J. Fagliari ◽  
M. Passipieri ◽  
H.T. Okuda ◽  
S.L. Silva ◽  
P.C. Silva
Keyword(s):  
Serum Protein ◽  
Acute Phase ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Clinical Signs ◽  
Control Group ◽  
Molecular Weights ◽  
Therapeutic Procedures

One hundred 6- to 12-month-old Nelore calves were allotted into control group (G1; 50 healthy calves) and photosensitization group (G2; n= 50). Blood samples were collected 12 to 24 hours after the onset of dermatitis (M1), and 15 to 30 days after that (M2), at time of resolution of clinical signs. Serum protein electrophoresis was performed by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Eighteen serum proteins with molecular weights ranging from 16,000 to 189,000 daltons (Da) were identified in all calves. In M1 and M2 serum concentrations of proteins with molecular weights of 115,000Da (ceruloplasmin), 61,000Da (a1-antitrypsin), 45,000Da (haptoglobin), and 40,000Da (acid glycoprotein) were significantly increased in calves. In conclusion, measurement of serum acute phase protein concentrations may be useful in monitoring the progression of bovine hepatogenous photosensitization, including guide probable alteration on therapeutic procedures.

scholarly journals The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2161 ◽  
Author(s):  
Wael M. El-Deeb ◽  
Ahmed M. Elmoslemany
Keyword(s):  
Diagnostic Accuracy ◽  
Acute Phase ◽  
Proinflammatory Cytokines ◽  
Diagnostic Performance ◽  
Pasteurella Multocida ◽  
Acute Phase Proteins ◽  
Eastern Region ◽  
Pneumonic Pasteurellosis ◽  
Amyloid A ◽  
Significant Difference

The goal of this study was to assess the diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. Blood samples were collected from 56 sheep (36 naturally infected withPasteurella multocidaand 20 healthy controls) belonging to one farm in Eastern region, Saudi Arabia. Serum samples were evaluated for acute phase proteins (Haptoglobin (Hp), serum amyloid A (SAA) and fibrinogen (Fb)), and the proinflammatory cytokines (interleukins (IL-1α, IL-1β, and IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-ϒ)). Additionally, nasopharyngeal swabs and bronchoalveolar lavages were collected from all animals for bacteriological examinations. Receiver operating characteristic curve was used to assess the diagnostic performance of each parameter. All parameters showed moderate to high degree of positive correlation with case-control status. There was no significant difference in the area under the curve (AUC) among acute phase proteins; however, both Hp and SAA showed better sensitivity and specificity than Fb. The proinflammatory cytokines (IL1-α, IL1-β, and IL6) showed similar and highly accurate diagnostic performance (AUC> 0.9), whereas IFN-ϒ was moderately accurate (AUC= 0.79). In conclusion, this study confirms the value of acute phase proteins and cytokines as diagnostic biomarkers of naturally occuring pneumonic pasteurellosis in sheep.

scholarly journals Serum acute phase proteins in Dirofilaria immitis and Wolbachia seropositive cats

2016 ◽  
Vol 19 (6) ◽  
pp. 693-696 ◽  
Author(s):  
Ana C Silvestre-Ferreira ◽  
Lisete Vieira ◽  
Hugo Vilhena ◽  
Jose J Cerón ◽  
Asta Tvarijonaviciute ◽  
...  
Keyword(s):  
Acute Phase ◽  
Dirofilaria Immitis ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Clinical Signs ◽  
Serum Amyloid ◽  
Amyloid A ◽  
Antibody Titres

Objectives The aim of this study was to characterise the response of acute phase proteins (APPs) in cats seropositive for Dirofilaria immitis and to its endosymbiont bacterium Wolbachia. Methods The APPs serum amyloid A (SAA), haptoglobin (Hp) and ceruloplasmin (Cp) were measured in 25 seropositive cats and in 16 healthy seronegative cats. Results SAA and Cp concentrations were significantly higher in animals with D immitis seropositivity that exhibited clinical signs related to the disease, and Hp was elevated in all D immitis-seropositive animals. There was no significant correlation between APPs and D immitis or Wolbachia species antibody titres. Conclusions and relevance An association between feline seropositivity to D immitis and APP response was demonstrated. Increases in serum SAA and Cp concentrations were related to D immitis-associated clinical signs, whereas Hp increased in all seropositive animals.

scholarly journals The electrophoretic pattern of serum proteins in dogs with babesiosis

2019 ◽  
Vol 88 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Csilla Tóthová ◽  
Branislav Lukáč ◽  
Marián Kadaši ◽  
Darina Baranová ◽  
Tatiana Weissová ◽  
...  
Keyword(s):  
Serum Proteins ◽  
Clinical Signs ◽  
Electrophoretic Pattern ◽  
Control Group ◽  
Babesia Canis ◽  
Total Proteins ◽  
Blood Samples ◽  
Γ Globulins ◽  
Healthy Dogs ◽  
G Ratio

This study was aimed at the evaluation of the electrophoretic pattern of serum proteins in dogs naturally infected with Babesia canis. Blood samples were collected from 37 dogs infected with B. canis and showing clinical signs consistent with the disease. The sick animals were classified as dogs with physiologic and decreased red blood cell (RBC) values. Twenty-five healthy dogs formed the control group. The concentrations of total proteins and protein fractions were measured in blood serum. The values of total proteins, albumin and albumin/globulin (A/G) ratio in dogs with babesiosis were significantly lower than in healthy ones (P < 0.001). In the globulin fractions, significantly higher relative concentrations of α1-, β1- and β2-globulins (P < 0.01), and non-significantly higher values of α2- and γ-globulins were found in dogs with babesiosis with a double α2-zone in six out of 37 animals. Marked differences were observed also between the two groups of sick animals, with significantly lower values of albumin and A/G ratio (P < 0.05), and significantly higher values of α1- and β1-globulins in dogs with decreased RBC (P < 0.05 and P < 0.01, respectively). Presented results indicate marked alterations in the electrophoretic pattern of serum proteins in dogs with babesiosis suggesting its usefulness for the evaluation of pathophysiological changes caused by the disease and for diagnostic of disease severity.

scholarly journals Peripheral blood gene expression of acute phase proteins in people with first episode psychosis

2017 ◽  
Vol 65 ◽  
pp. 337-341 ◽  
Author(s):  
Jie Yin Yee ◽  
Milawaty Nurjono ◽  
Wai Yee Ng ◽  
Stephanie Ruth Teo ◽  
Tih-Shih Lee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Phase ◽  
Peripheral Blood ◽  
Acute Phase Proteins ◽  
First Episode Psychosis ◽  
First Episode ◽  
Blood Gene Expression ◽  
Peripheral Blood Gene Expression ◽  
Episode Psychosis

scholarly journals Acute phase proteins in cats

2019 ◽  
Vol 49 (4) ◽  
Author(s):  
Rita Mourão Rosa ◽  
Lisa Alexandra Pereira Mestrinho
Keyword(s):  
Acute Phase ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Cell Damage ◽  
Clinical Signs ◽  
Blood Parameters ◽  
Acid Glycoprotein ◽  
Amyloid A ◽  
Clinical Interest ◽  
Technical Simplicity

ABSTRACT: Acute phase proteins (APP) are proteins synthesized and released largely by hepatocytes upon the occurrence of cell damage or invasion by microorganisms. This article reviews the use of APP in feline diseases, identifying their usefulness in the clinical setting, analyzing 55 published papers. Serum amyloid A, alpha-1 acid glycoprotein, and haptoglobin are the indicators pointed out by the authors as useful in monitoring the acute inflammatory response in cats. Although, APP measurement is still not routinely used in veterinary medicine, together with clinical signs and other blood parameters, was of clinical interest and applicability in diseases such as feline infectious peritonitis, pancreatitis, renal failure, retroviral and Calicivirus infections. Although, there are commercially available kits for dosing feline APP, assay standardization aiming technical simplicity, more species specificity and with less associated costs will allow routine use in feline practice, as it is done in the human field.

Studies on Acute Phase Proteins of Rat Serum. V. Effect of Induced Inflammation on the Synthesis of Albumin and α1-Acid Glycoprotein by Liver Slices

1975 ◽  
Vol 53 (4) ◽  
pp. 401-414 ◽  
Author(s):  
J. C. Jamieson ◽  
K. E. Morrison ◽  
D. Molasky ◽  
B. Turchen
Keyword(s):  
Acute Phase ◽  
Polypeptide Chain ◽  
Serum Proteins ◽  
Acute Phase Proteins ◽  
Rat Serum ◽  
Acid Glycoprotein ◽  
Liver Slices ◽  
Microsome Fraction ◽  
Lower Capacity ◽  
Acute Phase Serum

Liver slices from normal rats and those suffering from inflammation for 24–48 h were incubated with L-[14C]leucine or D-[I4C]glucosamine. Immunological techniques coupled with radioautography indicated that the microsome fraction prepared from slices contained the subcellular site of synthesis of the polypeptide chain of serum albumin, and the polypeptide and carbohydrate chains of α1-acid glycoprotein; both proteins were also present in the medium in labelled forms. The contents of albumin and α1-acid glycoprotein in the medium and in extracts of liver from experiments with liver slices from control rats and 8–72 h experimental rats were determined using the quantitative precipitin technique. There was a net increase in synthesis of both proteins when slices from control and experimental animals were used, the increase showing up in medium proteins. However, slices from livers from 8–72 h experimental rats had a greater capacity for synthesis of α1-acid glycoprotein and a lower capacity for synthesis of albumin than slices from livers from control rats, the greatest changes occurring with slices from 24 h experimental rats. Changes in synthetic capacities of liver slices from experimental rats for albumin and α1-acid glycoprotein were always accompanied by large increases in specific radioactivities of total medium proteins when experiments involved incubation of slices with L-[3H]leucine and D-[14C]glucosamine. It is suggested that the increase in specific radioactivities of medium proteins following incubation of liver slices from experimental rats with labelled leucine and glucosamine is a characteristic of the response of liver to inflammation, and reflects changes in the capacity of liver for the synthesis of α1-acid glycoprotein and other acute phase serum proteins.

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