Estimation Of Salivary Ph, Buffering Capacity And Flow Rate In Early Childhood Caries ( Ecc )

2020 ◽  
Vol 10 (2) ◽  
pp. 19-24
Author(s):  
Bidhata Ojha ◽  
Radha Baral ◽  
Dipshikha Bajracharya

Background: Renal failure is a process that expresses a loss of functional capacity of the nephrons, independently of its etiology. Although acute renal failure is reversible in the majority of cases, chronic renal failure presents a progressive course towards terminal renal failure. Hemodialysis is the most widely used technique leading to systemic alterations, oral complications and variations in the flow and composition of the saliva. The purpose of this study was to estimate salivary pH, buffering capacity, flow rate in chronic renal failure patients undergoing hemodialysis with its oral manifestation and caries prevalence. Methods: Saliva samples were collected from 40 patients with chronic renal failure undergoing dialysis and 40 healthy subjects after taking the informed consent. Saliva samples were then taken to the lab for processing. Salivary pH, buffering capacity and flow rate was estimated. DMFT was calculated. The statistical analysis was done using SPSS version 23. Results: A statistically significant rise in salivary pH, buffering capacity with decrease in flow rate was noted in chronic renal failure patients undergoing dialysis compared to controls. Furthermore, decrease in DMFT value was noted in CRF group despite of poor oral hygiene of the patient. We could also observe different oral manifestations in CRF group among which uremic fetor being the commonest. Conclusions: Our report suggest that saliva is noninvasive tool which act as an adjunct in diagnos­ing oral lesions and manifestations in CRF patients whose oral hygiene is often neglected with their reluctance in long term dental appointment in the middle of regular hemodialysis schedule.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aline Tavares Lima-Holanda ◽  
Emerson Tavares de Sousa ◽  
Marinês Nobre-dos-Santos ◽  
Carolina Steiner-Oliveira

AbstractThis quasi-experimental study sought to investigate if the mechanical control of biofilm (3-times-a-day) modifies the saliva’s ability to buffer the oral environment after 20% sucrose rinse (SR20%) in children with early childhood caries (ECC). Here, SR20% reduced the saliva’s pH in both groups and the mechanical control of biofilm had a greater effect on this parameter after SR20% in CF children. The mechanical control of biofilm evidenced a higher buffering capacity in CF children before SR20%, which was not observed after SR20%. Otherwise, the absence of mechanical control of biofilm showed that buffering capacity was comparable in the two groups before SR20%, whereas after SR20% the saliva’s buffering capacity of CF children was higher than ECC children. When biofilm was mechanically controlled, carbonic anhydrase VI activity did not change after SR20% whereas the absence of mechanical control of biofilm reduced this enzyme activity after SR20%. In conclusion, the mechanical control of biofilm did not change saliva’s ability to buffer the oral environment after SR20% in children with ECC. On the other hand, CF children appeared to regulate more effectively the saliva’s pH than ECC children while the absence of mechanical control of biofilm mediated their pH-modifying ability after SR20%.


Author(s):  
Dhanalakshmi Ravikumar ◽  
Pratibha Ramani ◽  
R. Gayathri

Background and Objectives: Early Childhood Caries (ECC) is a chronic disease of childhood affecting the primary dentition. It is also termed as nursing caries or baby bottle tooth decay. It has a multifactorial etiology and salivary parameters are considered as one of the important etiological factors of ECC. The present study was done to compare the salivary pH, flow rate and viscosity in children with and without ECC. Materials and Methods: It is a cross-sectional observational study conducted at Saveetha Dental college and Hospitals. Children between 3-6 years were examined and 20 caries free, 20 children with ECC and 20 children with Severe ECC were recruited for the study. The examination and sample collection were done by a single qualified Pediatric dentist. The samples were collected and analysed for salivary pH, flow rate and viscosity. Results: The results showed that there were no statistically significant differences in salivary pH levels in children with and without ECC. Statistically significant difference was evident in salivary flow rate and viscosity in caries-free, moderate ECC and severe ECC children. Conclusion: There is no significant difference in salivary pH levels in children with and without ECC. There is a significant difference in salivary flow rate and viscosity among caries free, moderate ECC and Severe ECC children Higher flow rate and low viscosity results in reduced caries occurance


2018 ◽  
Vol 63 (01) ◽  
pp. 63-68
Author(s):  
Efka Zabokova Bilbilova ◽  
Ana Sotirovska Ivkovska ◽  
Olivera Sarakinova ◽  
Olga Kokoceva Ivanovska ◽  
Natasha Stavreva

The aim of this study was to determine salivary pH, buffering capacity and calcium levels in caries-free and caries-active children. We examined 80 children of both genders, 15 years of age. Subjects were divided into four groups as follows: caries-free females, caries-active females, caries-free males, caries-active males; each group consisted of 20 subjects. The unstimulated saliva sample was collected by the spitting method and then pH, buffering capacity and calcium in saliva was measured. The results showed that mean level of buffering capacity of saliva was decreased significantly in the caries-active group as compared to caries-free group. The obtained data showed that the mean levels of pH and calcium were decreased in the caries-active group as compared to the caries-free group, but the difference was not statistically significant. The saliva with its constituents plays an important role in maintaining oral and especially dental health. Salivary pH values were found to be higher in the caries-free group. In our study, there was no significant correlation of pH values and caries activity with gender. Buffer capacity values were significantly lower in the caries-active group than in the caries-free group. There were significant differences when the groups were compared in the caries-active group where buffer capacity values were higher in boys than in girls. Calcium content of saliva was higher in the caries-free group. The results obtained in this study related to the values of the pH, buffering capacity and calcium in saliva, may serve as parameters for determining the caries risk patients, and accordingly to plan and carry appropriate caries preventive measures. Keywords: saliva, dental caries, pH, buffer capacity, calcium


2019 ◽  
Vol 43 (4) ◽  
pp. 239-243 ◽  
Author(s):  
Aline dos Santos Letieri ◽  
Liana Bastos Freitas-Fernandes ◽  
Ana Paula Canedo Valente ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Ivete Pomarico Ribeiro de Souza

Background: Our aim was to compare salivary levels of secretory immunoglobulin A (s-IgA) in children with early childhood caries (ECCG) and those who are caries-free (CFG) and verify these levels in a follow-up period after restorative treatment. Materials and methods: We selected 46 systemically healthy children in the complete primary dentition period, who were allocated into two groups: CFG (n = 23) and ECCG (dmf-s > 0; n = 23). Unstimulated whole saliva was obtained at baseline from both groups and during the follow-up period (7 days, 1, 2 and 3 months) in the ECCG group. The s-IgA was measured using an ELISA assay, and total protein was assessed using the Bradford method. We also evaluated the flow rate (mL/min), Streptococcus mutans and Lactobacillus spp. counting using selective media plaques. The data were submitted to statistical analysis using the software SPSS 20.0 (SPSS Inc, IL, USA) with a confidence interval set at 95%. Results: Salivary s-IgA levels were higher in baseline of ECCG than in CFG (p<0.05). No statistically significant differences were observed between s-IgA salivary levels at baseline and the evaluations after dental treatment in ECCG (p>0.05). However, we observed two different changes in s-IgA levels among participants: one group presented s-IgA reduction, and the other group demonstrated its maintenance. It was shown that patients from the ECCG group who presented a reduction in s-IgA levels during follow-up also showed a decrease in Streptococcus mutans and Lactobacillus spp. count (p<0.05), in contrast to patients who did not present this reduction. The flow rate and total protein were similar between groups (p>0.05). Conclusions: The present data support the idea that children with early childhood caries present higher levels of s-IgA in saliva than caries-free children. The restorative dental treatment does not have a significant influence on salivary levels of this immunoglobulin during the follow-up period.


2018 ◽  
Vol 42 (6) ◽  
pp. 445-449 ◽  
Author(s):  
Sudharani A Pyati ◽  
R Naveen Kumar ◽  
Vinod Kumar ◽  
N H Praveen Kumar ◽  
K M Parveen Reddy

Objectives: To measure and compare the levels of salivary flow rate, pH, buffering capacity, total protein, malondialdehyde (MDA) and total antioxidant capacity (TAC) between caries active and caries free children and to study the correlation between the DMFS/dfs score and above salivary parameters in caries active children. Study design: 50 caries active (DMFS/dfs ≥ 5) and 50 caries free (DMFS/dfs = 0) children aged between 6 to 12 years were included in the study. From all the children, unstimulated, mid-morning saliva samples were collected and salivary flow rate was calculated. Salivary pH, buffering capacity, total protein, MDA and TAC were measured. Results: The mean levels of salivary flow rate, pH, buffering capacity were significantly decreased (p < 0.05) and total protein, MDA and TAC were significantly increased (p < 0.05) in caries active children when compared to caries free controls. There was a proportionate decrease (p < 0.05) in salivary flow rate, pH and buffering capacity and proportionate increase (p > 0.05) in salivary total protein, MDA and TAC as DMFS/dfs score increased in caries active children. Conclusions: Significant alteration in the levels of salivary flow rate, pH, total proteins, MDA and TAC and their correlation with DMFS/dfs score in caries active children suggest, the levels of these physico-chemical properties of saliva can act as strong indicators of caries status in children.


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