Anti-biofilm activity of Azadirachta indica and Ocimum sanctum Aqueous Extract Combination against MRSA

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

Download Full-text

Effect of Supplementation of Tulsi (Ocimum sanctum) and Neem (Azadirachta indica) Leaf Powder on Diabetic Symptoms, Anthropometric Parameters and Blood Pressure of Non Insulin Dependent Male Diabetics

Studies on Ethno-Medicine ◽

10.31901/24566772.2009/03.01.02 ◽

2009 ◽

Vol 03 (01) ◽

Author(s):

Anita Kochhar

Keyword(s):

Blood Pressure ◽

Azadirachta Indica ◽

Ocimum Sanctum ◽

Anthropometric Parameters ◽

Leaf Powder ◽

Insulin Dependent

Download Full-text

Effect of aqueous extract of Azadirachta indica on rifampicin- induced jaundice in rabbits

World Journal of Advanced Research and Reviews ◽

10.30574/wjarr.2020.5.2.0422 ◽

2020 ◽

Vol 8 (2) ◽

pp. 205-212

Author(s):

Tunji Akande ◽

Timothy Olugbenga Ogundeko ◽

Solomon Oyewale Opajobi ◽

Samaila Garba Yaura ◽

Isiaiah Iorkyase Idyu ◽

...

Keyword(s):

Alkaline Phosphatase ◽

Acid Phosphatase ◽

Aqueous Extract ◽

Azadirachta Indica ◽

Total Bilirubin ◽

Alanine Transaminase ◽

The Body ◽

High Rate ◽

Care Needs ◽

Conjugated Bilirubin

High rate of dependence on medicinal plants for maintenance of health care needs over the decades in communities all over the world especially in Africa and Asia needs adequate scientific verification and authentication. Liver related diseases have however become a global problem. Jaundice was established on 9 of a total population of 12 rabbits of mixed sex, weighing 1.50kg - 2.34kg using an overdose pretreatment of Rifampicin (300mg/ kg bw) P.O. After 3 days, the Animals were administered with various doses of aqueous extract of Azadirachta indica (600, 750, 900mg/kg bw) P.O for each group of rats – A, B, C and D (control ), after which the animals were bled via marginal ear vein at zero hour, 7th and 14th days and the blood used for the following assays: Alkaline Phosphatase, Acid Phosphatase, Total Bilirubin, Conjugated Bilirubin and Alanine Transaminase. Experimental animals exhibited lack of appetite, reduced activity, scratching of the body, emesis, yellow coloration of the sclera, light-colored stool and a little dark urine over time. There was also increase in the levels of the biochemical parameters as the day increased. Aqueous extract of Azadirachta indica (Neem) exhibited increasing elevated levels of Alkaline Phosphatase (ALP), Acid Phosphatase (AP), Total Bilirubin (T/Bil), Conjugated Bilirubin (Conj/Bil), and slightly in Alanine Transaminase (ALT), thus exerted elevated hepatotoxicity on Rifampicin-induced Rabbits. Therefore, the traditional use of Azadirachta indica in management of jaundice should be discontinued.

Download Full-text

COMPARATIVE STUDY ON ANTIMICROBIAL ACTIVITY OF TULSI (OCIMUM SANCTUM) AND NEEM (AZADIRACHTA INDICA) METHANOL EXTRACT

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i12.28756 ◽

2018 ◽

Vol 11 (12) ◽

pp. 514

Author(s):

Vipul Kumar ◽

Anurag Chakraborty ◽

Manpreet Kaur ◽

Sony Pandey ◽

Manoj Kumar Jena

Keyword(s):

Antimicrobial Activity ◽

Azadirachta Indica ◽

Extraction Process ◽

Ocimum Sanctum ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Neem Extract ◽

E Coli ◽

Zones Of Inhibition ◽

Escherichia Coli Bacteria

Objective: This study was focused on to compare the antimicrobial activity of methanolic leaf extracts of tulsi and neem.Methods: We have chosen tulsi (Ocimum sanctum) and neem (Azadirachta indica) to compare their antimicrobial activity toward Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Phytochemical extracts have been made by the use of methanol as solvent and dried leaf powder by the cold maceration extraction process. Phytochemical analysis for some secondary metabolites has been done using standard protocols. Nutrient agar plates were inoculated with the above-mentioned microorganisms by spreading bacterial inoculum on the surface of the media. Wells (6 mm in diameter) were punched in the agar. The phytochemical extracts of neem and tulsi were allowed to diffuse into the medium, and after incubation of 24 h at 37°C, the zones of inhibition were observed.Results: Statistical analysis showed that tulsi was more effective toward S. aureus while neem was more effective toward E. coli. Minimum inhibitory concentration (MIC) of tulsi for both the microorganisms was 0.4 g/ml, and the MIC of neem for both the microorganisms was 0.2 g/ml. When both the tulsi and neem extract were mixed with each other for every concentration at equal volume, they have shown better effects in comparison to individual neem or tulsi extract and also the MIC got reduced to 0.2 g/ml for both the bacteria.Conclusions: Leaf extracts of both the A. indica and O. sanctum have shown antimicrobial activity against E. coli and S. aureus. A. indica has higher antimicrobial activity against S. aureus, whereas the O. sanctum was found to be more effective against E. coli (indicated by the zone of inhibition). When both the extracts were mixed with each other, they have shown a better effect toward both bacteria.

Download Full-text