Efficacy of Neem (Azadirachta Indica) and Tulsi (Ocimum Sanctum) Leaf Extracts Against Early Blight of Tomato

Author(s):  
B. Dheeba ◽  
R. Niranjana ◽  
P. Sampathkumar ◽  
K. Kannan ◽  
M. Kannan
Author(s):  
Vipul Kumar ◽  
Anurag Chakraborty ◽  
Manpreet Kaur ◽  
Sony Pandey ◽  
Manoj Kumar Jena

Objective: This study was focused on to compare the antimicrobial activity of methanolic leaf extracts of tulsi and neem.Methods: We have chosen tulsi (Ocimum sanctum) and neem (Azadirachta indica) to compare their antimicrobial activity toward Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Phytochemical extracts have been made by the use of methanol as solvent and dried leaf powder by the cold maceration extraction process. Phytochemical analysis for some secondary metabolites has been done using standard protocols. Nutrient agar plates were inoculated with the above-mentioned microorganisms by spreading bacterial inoculum on the surface of the media. Wells (6 mm in diameter) were punched in the agar. The phytochemical extracts of neem and tulsi were allowed to diffuse into the medium, and after incubation of 24 h at 37°C, the zones of inhibition were observed.Results: Statistical analysis showed that tulsi was more effective toward S. aureus while neem was more effective toward E. coli. Minimum inhibitory concentration (MIC) of tulsi for both the microorganisms was 0.4 g/ml, and the MIC of neem for both the microorganisms was 0.2 g/ml. When both the tulsi and neem extract were mixed with each other for every concentration at equal volume, they have shown better effects in comparison to individual neem or tulsi extract and also the MIC got reduced to 0.2 g/ml for both the bacteria.Conclusions: Leaf extracts of both the A. indica and O. sanctum have shown antimicrobial activity against E. coli and S. aureus. A. indica has higher antimicrobial activity against S. aureus, whereas the O. sanctum was found to be more effective against E. coli (indicated by the zone of inhibition). When both the extracts were mixed with each other, they have shown a better effect toward both bacteria.


2017 ◽  
Vol 5 (3) ◽  
pp. 336-341
Author(s):  
V. Jalander ◽  
M. Mamatha

Aqueous and ethanolic leaf extracts of six different medicinal plants such as Adhatoda vasica Nees, Azadirachta indica A. Juss., Catharanthus roseus (L.) G. Don., Cymbopogon citratus (DC.) Stapf., Eucalyptus globules Labill. and Ocimum sanctum L., were tested for their antifungal activity against Fusarium oxysporum f.sp. udum Butler, a wilt pathogen of pigeon pea [Cajanus cajan (L.) Millsp.] and Alternaria solani, a leaf spot pathogen of tomato (Lycopersicon esculentum Mill.). The leaf extracts (aqueous and ethanolic) prepared from six different plant leaves at different level of concentrations i.e. 5,10, 15 & 20% were incorporated in glucose nitrate liquid medium. The results were indicated that the ethanolic leaf extracts found good inhibitory activity than aqueous leaf extracts against plant pathogenic fungi tested. The ethanolic leaf extract prepared from Azadirachta indica was showed better efficacy against wilt pathogenic fungus and extract from E. globules found good inhibitory activity against leaf spot pathogen of tomato.


2012 ◽  
Vol 1 (2) ◽  
pp. 229 ◽  
Author(s):  
P. K. Baidoo ◽  
J. I. Adam

<p>The effects of ethanolic extract of neem, <em>Azadirachta indica</em> (Meliaceae), seeds and petroleum ether extract of <em>Lantana camara</em> leaves (Verbenaceae) on the populations of three cabbage pests, <em>Plutella xylostella</em>, <em>Brevicoryne brassicae</em> and <em>Hellula undalis</em> were studied. The study was conducted between January and April 2008. Extracts of the two plants were sprayed on cabbage plants to control these pests. A standard synthetic chemical insecticide (Mektin) was used as reference product. The experiment was conducted in a randomized complete block design and each treatment was replicated four times. The effects of plant extracts on the population dynamics of the pests’ species, the level of infestation and yield were assessed. Significantly more of the pests infested the control plants than the treated plants (<em>P</em>&lt; 0.01). The mean weight of cabbage heads on the sprayed plots was significantly heavier than that of the control unsprayed plots. The use of <em>A. indica</em> seeds and <em>L. camara</em> leaf extracts increased yield by 37.05% and 25.80%, respectively. Spraying the cabbage plants with the plant extracts significantly reduced the numbers of pests compared with the control plants. The use of these plant extracts can be incorporated into an overall control programme of these pests.</p>


2016 ◽  
Vol 42 (1) ◽  
pp. 41-48
Author(s):  
Trisha Saha ◽  
Shamim Shamsi

Anthracnose and blight were recorded on Houttuynia cordata Thunb. during April 2013 to December 2013. The isolated fungi from the symptomatic plants were identified as Alterneria alternata (Fr.) Keissler and Colletotrichum gloeosporoides (Penz.) Sacc. Ethanol leaf extracts of five plants viz.,Azadirachta indica L., Citrus limon L., Datura metel L., Sennaalata L. and Tagetes erecta L.were evaluated against the pathogenic fungi A. alternata and C. gloeosporoides at 5%, 10% and 20% concentrations in vitro. A. indica recorded as good inhibitor against the test fungi followed by C. limon, S. alata, D. metel and T.erecta. In vivo treatment also showed that A.indica is the most effective in controlling diseases at 10% concentration. The plants treated with A. indica were fresh and healthy up to one month of observation.J. Asiat. Soc. Bangladesh, Sci. 42(1): 41-48, June 2016


2019 ◽  
Vol 9 (2) ◽  
pp. 66-71
Author(s):  
Arfan ◽  
Ratnawati ◽  
Hasmari Noer ◽  
Lisa Indriani ◽  
Asli ◽  
...  

The purposes of this research the examine the effectiveness various concentrations neem leaf ekstract on the intensity of attack Liriomyza spp on shallot Lembah Palu variety . Field research was carried out in Guntarano Village, Tanantovea Subdistrict, Donggala Regency, Central Sulawesi.  This research conducted from February to April 2016 .The experiment was done using Randomized Complete Block  Design with three replications, consists of six  treatments, i.e. P0 : control (without aplication neem leaf ekstract); P1: neem leaf ekstract 10 gr /litre;  P2 : neem leaf ekstract 20 gr / litre; P3 : neem leaf ekstract 30 gr / litre; P4 : neem leaf ekstract 40 gr / litre; P5 : neem leaf ekstract 50 gr / litre. To find out the effect of threatment, the analysis of variance, and the real influence is continued with the standard  ά = 0.05 BNT test.  The results showed that use of seed and leaf extracts of neem plant can control Liriomyza spp attack, only 3,04 percent of attack rate and shallot Lembah Palu variety productivity is 3,02/area.


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