scholarly journals Stimulation of seed germination by humic substances: on the nature of the phenomenon

2019 ◽  
Vol 487 (3) ◽  
pp. 342-345
Author(s):  
S. A. Shoba ◽  
O. A. Salimgareeva ◽  
I. V. Gorepekin ◽  
G. N. Fedotov ◽  
A. L. Stepanov
Keyword(s):  
Seed Germination ◽  
Humic Substances ◽  
Seed Development ◽  
Seed Treatment ◽  
Podzolic Soil ◽  
Russian Plain ◽  
Wheat Seeds ◽  
Stimulation Of

The effect of soil compared to sand on the germination of wheat seeds and the development of their seedlings, as well as the effect of seed treatment with humic substances on their development in sand and soils was studied. For a number of the Russian Plain soils, it was shown that the soils inhibit seed germination significantly. It was established that pre-sowing treatment of seeds with humic preparations has a noticeable stimulating effect when sowing treated seeds in sod-podzolic soil and has no effect practically when sowing these seeds in sand. On the basis of the known phenomenon of soil allelotoxicity, it was suggested that the stimulating effect of humic substances on seed development is to limit the influence of allelotoxins on their development.

The effect of humic substances on germination and early growth of Lamb’s Quarters (Chenopodium album agg.)

Biologia ◽  
2011 ◽  
Vol 66 (3) ◽  
Author(s):  
Božena Šerá ◽  
František Novák
Keyword(s):  
Seed Germination ◽  
Humic Substances ◽  
Seed Development ◽  
Initial Phase ◽  
Chenopodium Album ◽  
Early Growth ◽  
Germination Test ◽  
Diverse Origin ◽  
Lamb’S Quarters ◽  
Germination And Growth

AbstractIn order to understand the biological activity of humic substances (HS), the effect of four humic acids (HA) and one fulvic acid (FA) on seed germination and early growth of cosmopolitan weed Chenopodium album agg. were tested. Humic substances of diverse origin were used, namely purified commercial HA, HA isolated from lignite, cambisol and podzol, and FA from mountain spruce forest soil. Data processing by two-way ANOVA has shown that type of the tested substances was a more important factor on seed development than used concentration. The major differences in germination and length of shoots were found in the first days of the experiment. Commercial and lignite HA stimulated the seed germination and growth, while podzol HA inhibited them. After this initial phase, all humic substances stimulated the seed development, but these stimulations were not significant (P < 0.05). At the end of germination test, the greatest stimulation effect (up to 20%) was achieved with the lignite HA.

scholarly journals THE EFFECT OF PURIFIED AMOUNT OF TRITERPENE GLYCOSIDES AND THEIR ENRICHED EXTRACT FROM LEAVES OF SILPHIUM PERFOLIATUM L. ON THE GERMINATION OF SEEDS OF WINTER WHEAT AND THE ACTIVITY OF CATALASE IN THEM

2021 ◽  
pp. 353-360
Author(s):  
Eleonora Sergeyevna Davidyants
Keyword(s):  
Seed Germination ◽  
Winter Wheat ◽  
Catalase Activity ◽  
Seed Treatment ◽  
Triterpene Glycosides ◽  
Growth Promoters ◽  
Oleanolic Acid Glycosides ◽  
Germinating Seeds ◽  
First Time ◽  
Wheat Seeds

The effect of purified amount of triterpene glycosides (PATG) containing, as major components, oleanolic acid glycosides – sylphiosides B, C, E, G and extract (E) enriched with them from Silphium perfoliatum L. (Asteraceae) leaves on seed germination and catalase activity (EC 1.11.1.6) in them on two varieties of winter wheat (Tritium aestivum L.) was studied. It has been shown that, the treatment of seeds with PATG solutions at concentrations of 0.0005 and 0.001% and E at concentrations of 0.2 and 0.4% increases the intensity of their swelling within 48 hours after soaking by 3.1–5.2% compared to the control, which leads to an earlier achievement of the threshold levels necessary for the activation of metabolic processes. As a result of a study of changes in catalase activity in germinating seeds of winter wheat after 1, 3 and 7 days after soaking, it was revealed that the greatest effect of seed treatment with TG preparations in manifested after 1 and 7 days of observation, its value in relation to the control respectively is 25–35% and 35–55% depending on the variety. The stimulating effect of TG preparations on catalase activity in germinating seeds of winter wheat was established for the first time. Under the influence of treatment with TG preparations the germination energy and laboratory germination of seeds increase by 3–8 and 3–6%, respectively, depending on the variety. The data obtained make it possible to consider the total preparation of TG isolated from leaves of S. perfoliatum as promising growth promoters for pre-sowing treatment of winter wheat seeds.

scholarly journals Changes in the morpho-physiological indicators of seed germination of wheat under the influence of ultrasonic exposure

2019 ◽  
pp. 38-42
Author(s):  
Sergey Sergeevich Tarasov ◽  
Alexander Pavlovich Veselov ◽  
Elena Konstantinovna Krutova
Keyword(s):  
Seed Germination ◽  
Seed Treatment ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Germination Capacity ◽  
Physiological Indicators ◽  
Initial Stage ◽  
Partial Dependence ◽  
Germination Stage ◽  
Wheat Seeds

The work is devoted to the study of the influence of ultrasound on the morphological and physiological-biochemical indicators of seed germination in wheat. The seeds were sonicated at the swelling stage, immediately after soaking and at the germination stage, 24 hours after the addition of water. The treatment was carried out for 5, 10 and 20 minutes, the controls were soaked, but not sonicated seeds. In the seeds and sprouts, the intensity of respiration, the activity of catalase and peroxidase, the length of the roots, the number of roots, the length of the shoots, the germination capacity, and the rate of germination were measured. A partial dependence on the effect of the investigated factor and the corresponding parameters studied is shown. Ultrasonic treatment had either a stimulating effect or an inhibitory effect. A statistically significant increase in the catalase activity was observed with 20 minutes of seed treatment at the swelling stage and at 10 and 20 minutes treatment at the germination stage. The five-minute action activated the activity of peroxidases. The intensity of respiration was higher in seeds treated during 10 and 20 minutes at the stage of swelling, and 5 minutes at the germination stage, a decrease in the intensity of respiration was observed in seeds treated with ultrasound for 20 minutes at the germination stage. The germination rate was enhanced by 10 minutes of action by ultrasound at the swelling stage. The analysis of morphometric indices revealed a positive dynamics for seeds treated with ultrasound for 10 minutes at the stage of swelling, negative values were recorded during seed treatment for 10 and 20 minutes at the germination stage. The point of stimulating action of ultrasound on wheat seeds in the region of 10 minutes at the initial stage of swelling was established.

scholarly journals Effect of pre-sowing treatment of spring wheat seeds with silicon-containing chelate microfertilizer on the growth and development of seedlings

2020 ◽  
Author(s):  
L.M. Anikina ◽  
◽  
O.R. Udalova ◽  
G.G. Panova ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Spring Wheat ◽  
Growth And Development ◽  
Seed Treatment ◽  
Wheat Varieties ◽  
Varietal Differences ◽  
Germination Energy ◽  
Sowing Seed ◽  
Wheat Seeds

The influence of pre-sowing seed treatment of spring wheat varieties ‘Ester’ and ‘Leningradskaya 6’ with silicon-containing chelate microfertilizer (SCM) on the seedlings’ growth and development was studied. Varietal differences of spring wheat in response to pre-sowing seed treatment with SCM were revealed. For both wheat varieties, this fertilizer stimulates the germination energy and germination itself. Thus, after SCM treatment at a concentration of 5 mg/l, there was a significant increase in ‘Ester’ seed germination energy (by 19 %) compared to control, as well as by 20–27 % in ‘Leningradskaya 6’ variety. More pronounced stimulating effect of SCM was observed for ‘Ester’ at a concentration of 1.0 mg/l; the same for ‘Leningradskaya 6’ – at a concentration of 3.0 and 5.0 mg/l.

Stimulation of Seed Germination by Humic Substances: On the Nature of the Phenomenon

2019 ◽  
Vol 487 (1) ◽  
pp. 105-107 ◽  
Author(s):  
S. A. Shoba ◽  
O. A. Salimgareeva ◽  
I. V. Gorepekin ◽  
G. N. Fedotov ◽  
A. L. Stepanov
Keyword(s):  
Seed Germination ◽  
Humic Substances ◽  
Stimulation Of

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

scholarly journals Non-TZF Protein AtC3H59/ZFWD3 Is Involved in Seed Germination, Seedling Development, and Seed Development, Interacting with PPPDE Family Protein Desi1 in Arabidopsis

2021 ◽  
Vol 22 (9) ◽  
pp. 4738
Author(s):  
Hye-Yeon Seok ◽  
Hyungjoon Bae ◽  
Taehyoung Kim ◽  
Syed Muhammad Muntazir Mehdi ◽  
Linh Vu Nguyen ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Zinc Finger ◽  
Root Length ◽  
Primary Root ◽  
Seedling Development ◽  
Ccch Zinc Finger ◽  
Family Protein ◽  
Wd40 Domain ◽  
Primary Root Length

Despite increasing reports on the function of CCCH zinc finger proteins in plant development and stress response, the functions and molecular aspects of many non-tandem CCCH zinc finger (non-TZF) proteins remain uncharacterized. AtC3H59/ZFWD3 is an Arabidopsis non-TZF protein and belongs to the ZFWD subfamily harboring a CCCH zinc finger motif and a WD40 domain. In this study, we characterized the biological and molecular functions of AtC3H59, which is subcellularly localized in the nucleus. The seeds of AtC3H59-overexpressing transgenic plants (OXs) germinated faster than those of wild type (WT), whereas atc3h59 mutant seeds germinated slower than WT seeds. AtC3H59 OX seedlings were larger and heavier than WT seedlings, whereas atc3h59 mutant seedlings were smaller and lighter than WT seedlings. Moreover, AtC3H59 OX seedlings had longer primary root length than WT seedlings, whereas atc3h59 mutant seedlings had shorter primary root length than WT seedlings, owing to altered cell division activity in the root meristem. During seed development, AtC3H59 OXs formed larger and heavier seeds than WT. Using yeast two-hybrid screening, we isolated Desi1, a PPPDE family protein, as an interacting partner of AtC3H59. AtC3H59 and Desi1 interacted via their WD40 domain and C-terminal region, respectively, in the nucleus. Taken together, our results indicate that AtC3H59 has pleiotropic effects on seed germination, seedling development, and seed development, and interacts with Desi1 in the nucleus via its entire WD40 domain. To our knowledge, this is the first report to describe the biological functions of the ZFWD protein and Desi1 in Arabidopsis.

scholarly journals Effect of seed treatment and foliar spray with fungicides in controlling black point disease of wheat

1970 ◽  
Vol 34 (3) ◽  
pp. 425-434 ◽  
Author(s):  
PK Malaker ◽  
IH Mian
Keyword(s):  
Economic Analysis ◽  
Grain Yield ◽  
Key Words ◽  
Seed Weight ◽  
Seed Treatment ◽  
Foliar Spray ◽  
The Other ◽  
Gross Margin ◽  
Black Point ◽  
Wheat Seeds

The efficacy of seed treatment and foliar spray with fungicides in controlling black point incidence of wheat seeds was evaluated in the field. Two seed treating fungicides, namely Vitavax-200 and Homai-80WP were used @ 0.25% of dry seed weight and foliar spray with Tilt-250EC (0.05%) was applied in six different schedules. Untreated and unsprayed controls were also maintained. Seed treatment with either Vitavax-200 or Homai-80WP significantly increased plant population and grain yield, but none of them was found effective in reducing black point incidence. On the other hand, foliar sprays with Tilt-250EC under all the spray schedules except spraying at 70 and 90 DAS significantly minimized the disease severity over unsprayed control. Among the different spray schedules, spraying at 30, 40, 50, 60, 70, 80, and 90 DAS appeared to be most effective, which was similar to spraying at 30, 45, 60, 75, and 90 DAS in reducing black point incidence and increasing grain yield. Economic analysis on yield advantage showed that the highest additional gross margin of Tk. 6120/ha with BCR 2.57 was obtained from five sprays applied at 30, 45, 60, 75, and 90 DAS. Key Words: Seed treatment, foliar spray, black point, wheat. DOI: 10.3329/bjar.v34i3.3968 Bangladesh J. Agril. Res. 34(3) : 425-434, September 2009

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