Changes in the morpho-physiological indicators of seed germination of wheat under the influence of ultrasonic exposure

The work is devoted to the study of the influence of ultrasound on the morphological and physiological-biochemical indicators of seed germination in wheat. The seeds were sonicated at the swelling stage, immediately after soaking and at the germination stage, 24 hours after the addition of water. The treatment was carried out for 5, 10 and 20 minutes, the controls were soaked, but not sonicated seeds. In the seeds and sprouts, the intensity of respiration, the activity of catalase and peroxidase, the length of the roots, the number of roots, the length of the shoots, the germination capacity, and the rate of germination were measured. A partial dependence on the effect of the investigated factor and the corresponding parameters studied is shown. Ultrasonic treatment had either a stimulating effect or an inhibitory effect. A statistically significant increase in the catalase activity was observed with 20 minutes of seed treatment at the swelling stage and at 10 and 20 minutes treatment at the germination stage. The five-minute action activated the activity of peroxidases. The intensity of respiration was higher in seeds treated during 10 and 20 minutes at the stage of swelling, and 5 minutes at the germination stage, a decrease in the intensity of respiration was observed in seeds treated with ultrasound for 20 minutes at the germination stage. The germination rate was enhanced by 10 minutes of action by ultrasound at the swelling stage. The analysis of morphometric indices revealed a positive dynamics for seeds treated with ultrasound for 10 minutes at the stage of swelling, negative values were recorded during seed treatment for 10 and 20 minutes at the germination stage. The point of stimulating action of ultrasound on wheat seeds in the region of 10 minutes at the initial stage of swelling was established.

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The Effect of High Concentrations of Ethylene on Seed Germination of Douglas Fir (Pseudotsugamenziesii (Mirb.) Franco)

Canadian Journal of Forest Research ◽

10.1139/x75-058 ◽

1975 ◽

Vol 5 (3) ◽

pp. 419-423 ◽

Cited By ~ 4

Author(s):

Carey Borno ◽

Iain E. P. Taylor

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Inhibitory Effect ◽

Germination Percentage ◽

Douglas Fir ◽

Control Treatment ◽

Continuous Exposure ◽

Short Term ◽

Short Term Exposure ◽

High Concentrations

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.

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Germination of dimorphic seeds of Suaeda moquinii under high salinity stress

Australian Journal of Botany ◽

10.1071/bt00020 ◽

2001 ◽

Vol 49 (2) ◽

pp. 185 ◽

Cited By ~ 36

Author(s):

M. Ajmal Khan ◽

Bilquees Gul ◽

Darrell J. Weber

Keyword(s):

Seed Germination ◽

Salt Marshes ◽

High Salinity ◽

Germination Rate ◽

Western United States ◽

Regression Analyses ◽

Salt Tolerant ◽

The Family ◽

Black Seeds ◽

Germination Stage

Suaeda moquinii (Torrey) Greene (desert blite), a succulent shrub in the family Chenopodiaceae, is widely distributed in salt marshes of the western United States. Suaeda moquinii produces dimorphic seeds (soft brown and hard black). Both types of seeds were collected from a salt marsh in Faust, Utah. Experiments were conducted to determine the seed germination responses of the black and brown seeds to salinity and temperature. Brown seeds were found to be one of the most salt tolerant at the germination stage when compared to other halophytes. Brown seeds germinated (30%) at 1000 mM NaCl, but only a few black seeds germinated (8%) at 600 mM NaCl. Seed germination occurred in most saline treatments at the lowest thermoperiod (5–15˚C) tested. In some salinity treatments (600, 800, 1000 mM), further increases in temperature resulted in progressively decreased seed germination. Brown seeds germinated better and had a higher germination rate (germination velocity) than black seeds at all thermoperiods. The highest rate of germination of black seeds occurred at the lowest thermoperiod (5–15˚C). Recovery of germination for black seeds when transferred to distilled water after being in various salinity treatments for 20 days was nearly complete (82–100%) at the lowest thermoperiod (5–15˚C) but decreased with increase in the temperature. Brown seeds recovered substantially (59–97%) from salinity at all thermoperiods. Regression analyses indicated significant differences between the germination recovery of the black and brown seeds.

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Allelopathy and Preliminary Separation of Allelopathic Substance in the Extracts of Wheat Seed Germination

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.468-471.565 ◽

2012 ◽

Vol 468-471 ◽

pp. 565-568 ◽

Cited By ~ 2

Author(s):

Jing Wen Bai ◽

Jing Wang ◽

Bo Tao ◽

Chun Hong Teng

Keyword(s):

Seed Germination ◽

Germination Rate ◽

Petri Dish ◽

Inhibitory Effect ◽

Chloroform Extract ◽

Wheat Seed ◽

Cucumis Sativus L ◽

Chloroform Phase ◽

Allelopathic Effects ◽

Allelopathic Substance

One variety of durum wheat (Triticum durum L.), ‘Longfu 30’, was choosen to study the allelopathic effects of seed germination extracts on other plants. The allelopathic effects of different polarity extracts were measured in terms of germination rate and germination speed on a test cucumber variety ‘Jinyou No.2’ (Cucumis sativus L.), and the allelopathic substance in chloroform extract was preliminarily separated with HSCCC and determined by GC-MS. The results showed that the allelopathy of wheat seed germination extracts varied with the polarity of solvent, and the substance in chloroform phase was the most active and showed a significantly inhibitory effect on seed germination of cucumber by 80.7% under Petri dish culture. The major allelopathic substance in wheat seed germination extracts were phenol, amine, piperazine, alcohols, esters and ketones.

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GERMINATION OF STORED SEEDS OF FOUR TREE SPECIES FROM THE TROPICAL DRY FOREST OF MORELOS, MEXICO

Botanical Sciences ◽

10.17129/botsci.96 ◽

2014 ◽

Vol 92 (2) ◽

pp. 281 ◽

Cited By ~ 3

Author(s):

Martha Cervantes ◽

Eliane Ceccon ◽

Consuelo Bonfil

Keyword(s):

Seed Germination ◽

Seed Size ◽

Storage Time ◽

Germination Rate ◽

Tropical Dry Forest ◽

Dry Forest ◽

Tropical Dry Forests ◽

Germination Capacity ◽

Dry Forests ◽

And Storage

Studies on propagation of trees of Tropical Dry Forests are scarce in Mexico, besides, the provenance of seeds used in reforestation programs is generally unknown or poorly addressed. Knowledge on seed germination patterns of different provenances, and how they change through time, is useful to identify adequate sources of seeds and to develop seed collection and storage programs under the low-tech conditions prevailing in most rural nurseries. We evaluated seed size variation and germination of stored seeds from three different provenances per species in Acacia bilimekii, Haematoxylum brasiletto, Lysiloma acapulcense, and L. divaricatum. Seeds were collected in four sites in the Tropical Dry Forest of Morelos, Mexico, and were stored at room temperature; seed size was estimated through the volume of 75 seeds per provenance/species. Seed germination tests were made periodically from six to 24 months after storage and the effects of provenance and storage time on germination were analyzed using Anovas. There were significant differences in seed size among provenances in all species, while the effect of provenance on germination rate was significant in three of them. Germination rate changed with storage time among species and provenances. After 24 months, germination capacity was still ≥ 50% in all provenances of the two Lysiloma species, but in A. bilimekii there were large differences among provenances. Mean germination capacity was low in H. brasiletto after six months. More research on seed germination and storage of a larger set of species and provenances is needed to restore the Mexican tropical dry forests.

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DAYA KECAMBAH BENIH ROTAN JERNANG (Daemonorops draco Blume) DENGAN BERBAGAI PERLAKUAN PERENDAMAN DALAM AIR

Jurnal Hutan Tropis ◽

10.20527/jht.v5i2.4365 ◽

2018 ◽

Vol 5 (2) ◽

pp. 120

Author(s):

Emmy Winarni ◽

Adistina Fitriani ◽

Purnomo Purnomo ◽

Sudin Panjaitan Panjaitan

Keyword(s):

Seed Germination ◽

Cold Water ◽

Germination Rate ◽

Water Immersion ◽

Preliminary Treatment ◽

Germination Capacity ◽

Soaking Time ◽

Dormancy Period ◽

Good Germination

Good germination of rattan seed (DaemonoropsdracoBlume) will increase the germination rate, germination rate, and germination rate. However, to germinate undergoes any obstacles because the seed of jernangrattan has a period of dormancy. The immersion in cold water or at normal temperature (28o - 32o C) is one way that can be used to break the dormancy period of the seed. This study aims to determine the long immersion to the value of germination of rattan seed jernang (D. dracoBlume). This study used 3 treatments and 1 replication. The treatment in this research is soaking time without immersion (control), soaking for 24 hours, and 48 hours. The result research is that the long immersion has the effect on germination of rattan seed jernang (D. dracoBlume). The results showed that the best percentage of rattan seed germination (D.dracoBlume) got the preliminary treatment with water immersion for 24 hours with a percentage of 80%. The best seed germination rate is at 48 hours of water immersion treatment at 33.89% / day. The highest germination rate was found in seeds treated by water immersion for 48 hours with an average of 0.0122% / day.Keywords : germination capacity; rotan jernangPerkecambahan benih rotan jernang (Daemonorops draco Blume) yang baik akan meningkatkan daya kecambah, laju perkecambahan,dan nilai perkecambahan. Namun demikian untuk mengecambahkan masih terdapat kendala, karena benih rotan jernang memiliki masa dormansi. Perendam dalam air dingin atau pada suhu normal (28o - 32o C) merupakan salah satu cara yang dapat digunakan untuk mematahkan masa dormansi benih. Penelitian ini bertujuan untuk mengetahui lama perendaman terhadap nilai perkecambahan benih rotan jernang (D. draco Blume ). Penelitian menggunakan 3 perlakuan dan 1 ulangan. Perlakuan pada penelitian ini adalah waktu perendaman yaitu tanpa perendaman (kontrol), perendaman selama 24 jam, dan 48 jam. Hasil penelitian yang didapat adalah lama perendaman berpengaruh terhadap perkecambahan benih rotan jernang ( D. draco Blume ). Hasil penelitian menunjukkan bahwa persentase perkecambahan benih rotan jernan ( D.draco Blume ) yang terbaik yaitu yang diberikan perlakuan pendahuluan dengan perendaman air selama 24 jam dengan persentase sebesar 80%. Laju perkecambahan benih yang terbaik adalah pada perlakuan perendaman air selama 48 jam yaitu sebesar 33.89 %/hari. Nilai perkecambahan tertinggi terdapat pada benih yang diberikan perlakuan perendaman air selama 48 jam dengan rata – rata sebesar 0.0122 %/hari.Kata kunci : daya perkecambahan; rotan jernang

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Germination Behavior of Rauvolfia serpentine (L.) Seeds with the use of Different Seed Treatments

Indian Journal of Agricultural Research ◽

10.18805/ijare.a-5624 ◽

2021 ◽

Author(s):

Amit Kumar Tiwari ◽

Prawal P.S. Verma ◽

Dipender Kumar ◽

Sonveer Singh ◽

Rakesh Kumar ◽

...

Keyword(s):

Seed Germination ◽

Seedling Growth ◽

Seed Treatment ◽

Inhibitory Effect ◽

Germination Percentage ◽

Hot Water ◽

Cotton Cloth ◽

Cow Dung ◽

Seed Treatments ◽

Seed Propagation

Background: Sarpagandha is an indigenous medicinal herb of Indian continent. It has many medicinal properties. Due to increasing demand of Sarpagandha, its exploitation is increasing continuously but for lack of cultivation, this plant has been listed in endangered category. Commercially, it is propagated through seeds but the main barrier of seed propagation is its irregular and low germination. Hence, the current study was undertaken to study the effect of growing media and seed treatment methods on seed germination and seedling growth of Sarpagandha to eliminate the inhibitory effect of the hard stony endocarp.Methods: Seed germination and seedling growth experiments of Sarpagandha were carried out with following different seed treatments, T1 (Control), T2 (Water soaking for 24 hours), T3 (water soaking for 48 hours), T4 (Water soaking for 24 hours + cotton cloth wrapping for 24 hours), T5 (GA3 200 ppm), T6 (FYM treatment for 24 hours), T7 (FYM treatment for 48 hours), T8 (Hot water treatment) and T9 (Cow dung treatment for 24 hours). Experiment was laid out in CRD. The data recorded on the various parameters were analyzed at 5% level of significance by using ANOVA.Result: Results indicated highest germination percentage (51), lowest mortality percent (49), minimum days for initiation of germination (23), minimum days taken for germination completion (33), highest speed of germination (3.77), maximum vigour index (1441.44), highest root (11.27 cm) and highest shoot length (17 cm) in T5 (GA3 200 ppm) followed by T3 (water soaking for 48 hours). Indicating, T5 (GA3 200 ppm) to be best seed treatment for higher germination and subsequent seedling growth of Sarpagandha (Rauvolfia serpentine L.).

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The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage

Jurnal Wasian ◽

10.20886/jwas.v8i2.5997 ◽

2021 ◽

Vol 8 (2) ◽

pp. 133-143

Author(s):

Iskandar Siregar ◽

◽

Riki Ramdhani ◽

Evayusvita Rustam ◽

Dede Sudrajat ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Germination Rate ◽

Seed Viability ◽

Germination Capacity ◽

Peg 6000 ◽

Vigor Index ◽

Fine Bubble ◽

Initial Seed ◽

Environmental Air

Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 Mpa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62%, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.

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Effect of light quality on Tabebuia rosea seed germination

Universitas Scientiarum ◽

10.11144/javeriana.sc20-2.elqt ◽

2014 ◽

Vol 20 (2) ◽

pp. 191

Author(s):

Sofía Basto ◽

Claudia Ramírez

Keyword(s):

Seed Germination ◽

Light Quality ◽

Germination Rate ◽

Germination Capacity ◽

Germination Speed ◽

Tabebuia Rosea ◽

Germination Value ◽

Positive Effect ◽

Effect Of Light ◽

Peak Value

Tabebuia rosea germination capacity and rate have been examined under light and dark conditions. However, seeds responses to light quality, in particular to short and mid-wavelengths, are not well known. We hypothesized that short wavelengths would have a positive effect on rosea germination. The effects of short, mid and long-wavelengths on germination capacity, rate (germination rate index, germination rate R50, a devised index of germination rate R50´ and peak value), mean daily germination and germination value were evaluated. Light quality had little effect on germination capacity; it was reduced only in darkness. Seeds under short and mid-wavelengths germinated faster (germination rate) than under any other light treatments. We concluded that althoughlt T. rosea seeds respond to all light quality treatments, the short and mid-wavelengths are the cue for increasing germination speed. For synchronizing germination, short and mid-wavelengths transmitted by red cellophane paper can be used as a pre-germinative treatment for restoration and silvicultural purposes.

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Effects of Osmotic Stress Induced by Polyethylene Glycol (Peg) 6000 and Mannitol on Seed Germination and Seedling Growth of Durum Wheat

Journal of Bioresource Management ◽

10.35691/jbm.1202.0195 ◽

2021 ◽

Vol 8 (3) ◽

pp. 57-66

Author(s):

Ratiba Bousba ◽

Rabah Bounar ◽

Narimene Sedrati ◽

Randa Lekhal ◽

Chourouk Hamla ◽

...

Keyword(s):

Polyethylene Glycol ◽

Seed Germination ◽

Durum Wheat ◽

Crop Production ◽

Germination Rate ◽

Inhibitory Effect ◽

Wheat Genotype ◽

Peg 6000 ◽

Germination Parameters

Seed germination is generally the critical step in seed establishment and thus the determination of successful crop production. This study was focused at examination of the biochemical and germination parameters effected by low water potential which was generated by polyethylene glycol (PEG) 6000 and mannitol, related to drought stress and growth of Waha durum wheat genotype. Two tests were carried out in a growth chamber; the first comprises seed germination into Petri dishes in the presence of different concentrations of the two osmoticums (0, 5, 10, 15 and 20 % of PEG6000 and mannitol). The second test was carried out in nutrient solution BD medium. Our results shows that Both PEG -6000 and mannitol reduced germination. Therefore, a rapid increase was observed in the rate of germination both for the control plants and the plants subjected to a concentration of 5 g/L and 10 g/L and changes in proportion to the time. For the concentration of 15 g/L and 20 g/L, this phase is very short, which explains the reduced germination rate due to the inhibitory effect of the two osmoticums on germination. In this study, PEG-6000 treatments resulted in an increase of some proteins and a decrease of others. Waha displayed 12 bands for control plants, 40 bands for PEG-6000 stressed plants (all treatments) and 35 bands for mannitol treatments.

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Salinity Effect on Seed Germination and Growth of Two Warm-season Native Grass Species

HortScience ◽

10.21273/hortsci.47.4.527 ◽

2012 ◽

Vol 47 (4) ◽

pp. 527-530 ◽

Cited By ~ 9

Author(s):

Qi Zhang ◽

Kevin Rue ◽

Sheng Wang

Keyword(s):

Seed Germination ◽

Salinity Tolerance ◽

Vegetative Growth ◽

Germination Rate ◽

Dry Weight ◽

Mature Stage ◽

Salt Tolerant ◽

Blue Grama ◽

Salinity Levels ◽

Germination Stage

Salinity tolerance of five buffalograss [Buchloe dactyloides (Nutt.) Englem.] cultivars (Texoka, Cody, Bison, Sharp's Improved II, and Bowie) and three blue grama [Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths] ecotypes (‘Lovington’, ‘Hachita’, and ‘Bad River’) was determined during in vitro seed germination and vegetative growth in a hydroponic system. Seeds were germinated on 0.6% agar medium supplemented with NaCl at 0, 5, 10, 15, and 20 g·L−1. Salinity reduced the final germination rate (FGR) and daily germination rate (DGR). Similarly, shoot dry weight (SDW), longest root length (LRL), and percentage of green tissue (PGT) of mature grasses declined with increasing salinity levels (NaCl = 0, 2.5, 5, 7.5, and 10 g·L−1). However, root dry weight (RDW) was not significantly affected by salinity. Blue grama exhibited a lower reduction in FGR and DGR than buffalograss at salinity levels lower than 10 g·L−1. Germination of all buffalograss cultivars and ‘Hachita’ blue grama was inhibited at salinity levels of 15 and 20 g·L−1 NaCl. However, buffalograss was more salt-tolerant than blue grama at the vegetative growth stage. Variations of salinity tolerance were observed within buffalograss cultivars and blue grama ecotypes, especially during the seed germination stage. Overall, buffalograss appeared to be salt-sensitive during germination but moderately salt-tolerant at the mature stage. However, blue grama was more salt-tolerant at the germination stage than the mature stage. Noticeable differences in salinity tolerance were observed between different germplasms. Therefore, salt tolerance of buffalograss and blue grama may be improved through turfgrass breeding efforts.

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