Isolation of rare Genera of actinomycetes — antibiotic producers from soils using Aloe Arborescens juice

The search for new antibiotics is an urgent problem due to the spread of resistance to existing antibacterial drugs in pathogenic microorganisms. Actinomycetes are producers of a large number of antibiotics used in medicine. Most antibiotics are isolated from actinomycetes of the Streptomyces genus, while rare genera of actinomycetes can be the producers of new antibiotics.The aim of the study is to investigate the effect of the biological substances complex present in aloe juice on the growth stimulation of rare genera of actinomycetes.Material and methods. Objects: samples of sod-podzolic soil and chernozem. The standard method of sowing soil suspensions on oat agar and Gause medium No. 2 was used to isolate actinomycetes. Chemotaxonomic properties were determined using the methods of ascending thin-layer chromatography on a cellulose layer. The generic identity of cultures was determined using Bergey’s manual and materials comparing the composition of cell walls of actinobacteria. DNA PCR with standard 27f and 1492r primers, as well as Sanger sequencing, were performed to study genosystematic features. Antibiotic activity was determined against the test microorganisms: Staphylococcus aureus ИНА 00985 (FDA 209P), Staphylococcus aureus ИНА 00761 (MRSA), Staphylococcus aureus ИНА 00762 (УФ- 2), Micrococcus luteus ATCC 9341, Bacillus subtilis ATCC 6633, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Saccharomyces cerevisiae ИНА 01042.Results. A total of 527 actinomycete cultures were isolated from samples of sod-podzolic soil and chernozem with the addition of aloe juice; their phylogenetic position was determined. The dominant actinomycetes in the studied soil samples are the representatives of the genus Streptomyces. Bacteria of the genus Micromonospora take the second place by the number of isolated cultures. Rare genera of actinomycetes have also been identified: Nonomuraea, Streptosporangium, Nocardia, Actinomadura, Actinocorallia, Pseudonocardia, Amycolatopsis, Saccharomonospora, Saccharopolyspora, Promicromonospora, Kribbella. It was determined that the isolated cultures possess antibiotic activity against test microorganisms.Conclusion. It is advisable to use aloe juice after subjecting the leaves to biostimulation to isolate actinomycetes from the soil and identify their biodiversity.

AGROENERGY EFFICIENCY OF IMPROVED TECHNOLOGIES FOR CREATION AND USE OF ALFALFA-CEREAL HAYMAKING IN THE NON-CHERNOZEM ZONE

On the basis of field experience in the creation of alfalfa-grass stands on sod-podzolic soil of the Non-Chernozem zone, a high agroenergetic efficiency of improved technologies has been established, including two zoned varieties of alfalfa changeable (Lugovaya 67 and Pastbishchnaya 88) in combination with agrotechnical methods (change in the composition of leguminous species in the previous period and pre-sowing inoculation of alfalfa seeds with complementary strains of Sinorhizobium meliloti). As a result of the application of the agro-energy method for assessing the production of exchangeable energy and total anthropogenic costs in uniform indicators according to the international SI system (GJ/ha), their payback was established — 8–11 times. This was achieved due to a high share of natural factors (88–91% of total costs), additional nitrogen input into the production process due to increased symbiotic fixation (141–171 kg/ha per year) and long-term use — for 7 years. The productivity of 1 hectare was 62–64 GJ/ha metabolic energy, the collection of protein was 956–1120 kg/ha, the saving of anthropogenic costs due to the exclusion of the use of nitrogen fertilizers was 17–21 GJ/ha.

THE RESULTS OF THE STUDY OF THE AFTEREFFECT OF THE USE OF BIOMELIORANTS TO INCREASE THE PRODUCTIVITY AND BIOLOGICAL ACTIVITY OF DEGRADED SOD - PODZOLIC SOIL

The paper evaluates the effectiveness of the aftereffect of the use of biomeliorants (effluent and biocompost) to increase the productivity of soils in degraded reclaimed lands, carried out under the conditions of a lysimetric experiment on a stationary site, when growing annual grasses (vetch-oat mixture). It was experimentally established that effluent and biocompost based on sewage sludge and manure had a positive effect (in the first year of aftereffect) on the productivity and biological activity of soddy-podzolic sandy loam soil of fallow reclaimed agricultural lands.

Organic matter of sod-podzolic soil after transition to a fallow state

Soil plays a crucial role in carbon sequestration in terrestrial ecosystems. It is known that the strengthening of carbon sequestration processes occurs with a decrease in the intensity of soil treatments. The study of changes in organic matter and physical properties of sod-podzolic soil 16 years after the transition from arable soils to a fallow state against the background of weak water erosion was carried out. A significant increase in the content and reserves of total carbon in fallow soil compared to arable soil was found, mainly due to carbon of the light fraction. On arable soil, the content of the light fraction in the lower part of the field was significantly higher than in the upper part, due to the washing away of light particles as a result of erosion, these differences were smoothed out on fallow soil. There are no significant changes in the density, density of the solid phase and total porosity in fallow soil at this stage of succession, compared with arable soil. In fallow soil, the content of macro-aggregates (including water-bearing ones) was noticeably higher, and the share of micro-aggregates was lower than in arable soil.

Effect of Different Doses of Ash from Biomass Combustion on the Development of Diatom Assemblages on Podzolic Soil under Oilseed Rape Cultivation

The aim of the study was to determine the effect of fertilization with various doses of ash from biomass combustion (balanced to the amount of K2O introduced into the soil) on the growth of representatives of soil microorganisms, i.e., diatoms. In a one-factor field experiment (control, NPK, 100, 200, 300, 400, 500 kg/ha), soil samples were collected from the 0–5 cm layer of podzolic soil under the cultivation of winter oilseed rape (Brassica napus L. var. napus) at the end of August 2019 and 2020. The biomass combustion ash used for soil fertilization was characterized by an alkaline reaction (pH = 12.83 ± 0.68) and high levels of basic macroelements required for proper plant growth and development. The particle size distribution in each plot was identified as loamy silt (pgl). Before the experiment (autumn 2018), the soil exhibited an acidic reaction (pHH2O = 5.8), low conductivity (EC = 68 µS), and 19.09% moisture at the 0–5 cm level. In total, 23 diatom species were identified in the material collected from the topsoil in all variants of the experiment. Hantzschia amphioxys, Mayamaea atomus, Mayamaea permitis, Nitzschia pusilla, Pinnularia obscura, Pinnularia schoenfelderi, and Stauroneis thermicola were the most abundant populations.

Chicken droppings influence on sod-podzolic soil fertility change studying experience

The aim of the research was to study the effect of fresh chicken droppings on the dynamics of the physical-chemical and agrochemical properties of sod-podzolic soil and the yield of grain crops. In the sod-podzolic light loamy soil of the Yaroslavl region in the layer of 0-0.20 m in May, fresh chicken droppings were embedded at the dose of 120 t/ha. By autumn, in the 0-0.40 m layer of pHKc1 increased from 4.53-4.99 to 5.38-7.09, the content of mobile phosphorus and exchangeable potassium increased by 2.0-2.4 times, nitrate and ammonium nitrogen - 3-5 times. The content of nitrates in the soil has exceeded the Maximum Allowable Concentration level. The grain yield of the spring wheat studied varieties, triticale and barley varied from 6.7 to 9.5 t/ha, chaffy oats - from 5.7 to 5.9, hulless oats - from 4.4 to 4.6 t/ha. At the beginning of the growing season of crops in the 0-0.40 m layer, the stocks of nitrate nitrogen were close to the stocks of the autumn observation period of 2019, and ammonium nitrogen decreased by 285 kg/ha.

Fluxes of 15N, soil nitrogen in sod-podzolic soil in spring wheat crops under inoculation with Rhyzoagrin

In a microfield experiment using a labeled nitrogen 15N fertilizer on sod-podzolic light loamy soil for 11 years during the cultivation of spring wheat and the use of a biological product based on a strain belonging to the genus Agrobakterium radiobakter (strain 204), nitrogen flows in the fertilizer – soil – plant system were studied. The utilization rate of spring wheat labeled with 15N ammonium nitrate averaged 40% of the amount applied. The absolute size of the immobilization in the structure of the labeled nitrogen balance of the mineral fertilizer was 27-30%. Inoculation with Rhyzoagrin does not significantly affect the nitrogen immobilization of the mineral fertilizer. The gaseous losses of labeled nitrogen of the mineral fertilizer were 33% of those applied to the background of the phosphorus and potash fertilizers and decreased with inoculation with Rhyzoagrin to 29%. The use of an integral assessment showed that the agroecosystem functioned in the resistance mode on sod-podzolic light-loamy soil when using mineral nitrogen fertilizer. The dependence of the indicator RI: M, % of the HTC (hydrothermal coefficient) expressed by the equation y = -24, 506x2 + 43,462 x + 15,251 at R2 = 0.7416 is established.

New methods of cleaning soil from heavy metals

The purpose of the work was to study new methods of cleaning soil from heavy metals. In this work, it was proposed to extract heavy metals Cd, Zn, Fe, Cu, Mn, Pb from soils using the method of biological purification. For this, we recommended to carry out preliminary treatment of soils with complexones and to carry out additional introduction of biophilic elements into complex compounds. The possibility of using electromechanical treatment for removing heavy metals from soils after a preliminary increase in the mobility of ions by acidification and the use of complexation reactions is shown. The experiments were carried out on the plants of sowing oat Avena sativa L. variety Yakov when grown in laboratory conditions on sod-podzolic soil with the introduction of soluble salts of heavy metals into the soil. It was noted that the largest amount of heavy metals is concentrated in the root system of plants. When growing oats in the field, the concentration of metals in the grain was assessed: Mn - 30-35 ppm, Fe - 55-65 ppm, Cu - 4-5 ppm, Zn - 30-35 ppm, Cd, Pb < 3 ppm.