scholarly journals Anti-Cancer Effect of Angelica Sinensis on Women’s Reproductive Cancer

2012 ◽  
Vol 2 (6) ◽  
pp. 242 ◽  
Author(s):  
Hong-Hong Zhu ◽  
Guo-Hui Huang ◽  
Patricia L. Tate ◽  
Lyndon L. Larcom
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Uv Light ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Dose Effect ◽  
Angelica Sinensis ◽  
Reproductive Disorders ◽  
Anti Cancer ◽  
Mcf 7

Objective: Danggui, the root of Angelica Sinensis, has traditionally been used for the treatment of women’s reproductive disorders in China for thousands of years. This study was to determine whether Danggui have potential anti-cancer effect on women’s cancer and its potential mechanism. Methods: Danggui was extracted by ethanol. The Cell Titer 96® Aqueous Non-Radioactive Cell Proliferation Assay was used to compare the effects of Danggui on human breast (MCF-7 and 7368) and cervical (CaSki and SiHa) cancer cells with its effects on normal fibroblasts (HTB-125). A revised Ames test was used to test for antimutagenicity. The standard strains of Salmonella typhimarium (TA) 100 and 102 were used in the test. Methyl methane sulfonate (MMS) and UV light were used as positive mutagen controls and ethanol and double distilled water (DDW) as controls. The SAS statistical software was used to analyze the data. Results: Danggui was found to be much more toxic to all cancer cell lines tested than to normal fibroblasts. There was a significant negative dose-effect relationship between Danggui and cancer cell viability. Average viability of MCF-7 was 69.5%, 18.4%, 5.7%, 5.7%, and 5.0% of control for Danggui doses 0.07, 0.14, 0.21, 0.32, and 0.64 ug/ul, respectively, with a Ptrend < 0.0001. Half maximal inhibitory dose (ID50) of Danggui for cancer cell lines MCF-7, CaSki, SiHa and CRL-7368 was 0.10, 0.09, 0.10 and 0.07 ug/ul, respectively. For the normal fibroblasts, ID50 was 0.58 ug/ul. At a dose of 0.32 ug/ul, Danggui killed over 90% of the cells in each cancer cell line, but at the same dose, only 12.3 % of the normal HTB-125 cells were killed. Revertants per plate of TA 100 decreased with the introduction of increasing doses of Danggui extracts with a Ptrend < 0.0001 when UV light was used as a mutagen. There was no difference in revertants per plate between ethanol and DDW control groups. Conclusions: Danggui could be used as a safe and effective adjuvant therapy to prevent and treat breast and cervical cancers. Anti-cancer effects may be due to its anti-mutagenicity. Danggui should be investigated as a potential adjuvant anti-cancer therapy for women’s cancer treatment and prevention of recurrence. Key words: Angelica Sinensis, Danggui, cancer, women’s reproductive disorders

scholarly journals 8-Hydroxycudraxanthone G Suppresses IL-8 Production in SP-C1 Tongue Cancer Cells

2014 ◽  
Vol 9 (1) ◽  
pp. 1934578X1400900
Author(s):  
Arlette S. Setiawan ◽  
Roosje R. Oewen ◽  
Supriatno ◽  
Willyanti Soewondo ◽  
Sidik ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Cancer Cell ◽  
Therapeutic Intervention ◽  
Tongue Cancer ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Garcinia Mangostana ◽  
Anti Cancer ◽  
Cancer Activity

Production of IL-8 primarily promotes angiogenic responses in cancer cells, which lead to favorable disease progression. Suppressing this production may, therefore, be a significant therapeutic intervention in targeting tumor angiogenesis. This study aimed to evaluate the reduction effects of xanthones in cancer cell lines. Nine known prenylated xanthones (1–9), isolated from the pericarp of Garcinia mangostana Linn (GML), were tested for their ability to suppress IL-8 (interleukin-8) of the SP-C1 (Supri's Clone 1) tongue cancer cell line. Of these compounds, 8-hydroxycudraxanthone-G (4) suppressed IL-8 within 48 hours. This is the first report of 8-hydroxycudraxanthone G suppressing the production of IL-8 (45% at 15.7 μg/mL in 48 hours). These results suggest that the prolonged suppression of IL-8 production by cancer cell lines is concerned in the anti-cancer activity of 8-hydroxycudraxanthone.

Anti-cancer activity of pegylated liposomal trans-anethole on breast cancer cell lines MCF-7 and T47D

2015 ◽  
Vol 37 (7) ◽  
pp. 1355-1359 ◽  
Author(s):  
Shahedeh Shahbazian ◽  
Azim Akbarzadeh ◽  
Sepideh Torabi ◽  
Mansour Omidi
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Anti Cancer ◽  
Cancer Activity ◽  
Mcf 7

Study of anti-cancer properties of green silver nanoparticles against MCF-7 breast cancer cell lines

2016 ◽  
Vol 5 (2) ◽  
Author(s):  
Shweta Rajawat ◽  
Rajnish Kurchania ◽  
Katherukamen Rajukumar ◽  
Shreyas Pitale ◽  
Sonali Saha ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Cell Lines ◽  
Cancer Cell ◽  
Black Tea ◽  
Cancer Cell Lines ◽  
Electrolytic Deposition ◽  
Anti Cancer ◽  
Average Size ◽  
Tea Leaf ◽  
Mcf 7

AbstractIn the present work, silver nanoparticles were synthesized using an easy, simple, and environment-friendly method based on principles of green chemistry in the absence of a sophisticated laboratory, and their anti-cancer properties were studied. Silver nanoparticles were synthesized using electrolytic deposition. As-synthesized nanoparticles were capped using black tea leaf extract. MTT assay was used to investigate anti-cancer activity. X-ray diffraction graphs show highly pure as-synthesized silver nanoparticles. Transmission electron microscopy images show well-dispersed spherical nanoparticles, with an average size of 9 and 15 nm, corresponding to different values of parameters used in the synthesis. For the MCF-7 cancer cell lines, 100% growth inhibition is obtained. The 50% growth inhibition concentration values against MCF-7 cancer cell lines were obtained at 70- and 30-fold dilutions of colloidal silver of almost the same concentration, 178 μg/ml, for both configurations. Silver nanoparticles can be synthesized, and their morphology can be tuned using the electrolytic deposition method with black tea leaf extract as capping agent. Silver nanoparticles with an average size of 9 nm are more effective those with an average size of 15 nm. The synthesis method is faster, cheaper, and environment friendly and renders a treatment option that can have high accessibility, reduced harmful side effects, and increased economic benefits.

Guide for Selection of Relevant Cell Lines During the Evaluation of new Anti-Cancer Compounds

2018 ◽  
Vol 18 (8) ◽  
pp. 1072-1081
Author(s):  
Angel J. Ruiz-Moreno ◽  
Patricia Torres-Barrera ◽  
Mireya Velázquez-Paniagua ◽  
Alexander Dömling ◽  
Marco A. Velasco-Velázquez
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Activity Analysis ◽  
Anti Cancer ◽  
Line Selection ◽  
Cell Line Selection ◽  
Selection Of

Background: Human cancer cell lines are valuable models for anti-cancer drug development. Although all cancer cells share common biological features, each cancer cell line has unique genotypic/ phenotypic characteristics that affect drug response. Thus, the information obtained with a specific cancer cell line cannot be easily extrapolated to other cancer cells. Consequently, cell line selection during experimental design is critical for providing proper and clinically relevant structure-activity analysis. Methods: Herein, we critically review the use of cancer cell lines as tools for activity analysis by comparing two different scenarios: i) the use of multiple cancer cell lines, with the NCI-60 Program as the most representative example; and, ii) the selection of a single cell line with specific biological characteristics that match the rationale of compound design. Results: Considering that most laboratories evaluate the activity of new compounds using few cell lines, we provide a systematic strategy for selection based on the expression levels and genetic status of the target and the effectiveness of target inhibition or silencing. We exemplify the use of public databases for data retrieval and analysis as well as the critical comparison of such information with published results. Conclusion: This approach refines cell line selection, avoiding the perpetuation of published poor selection and enhancing the relevance of the results.

scholarly journals Synthesis, Anti-Cancer and Anti-Migratory Evaluation of 3,6-Dibromocarbazole and 5-Bromoindole Derivatives

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2686 ◽  
Author(s):  
Krystal M. Butler-Fernández ◽  
Zulma Ramos ◽  
Adela M. Francis-Malavé ◽  
Joseph Bloom ◽  
Suranganie Dharmawardhane ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Migration Activity ◽  
Antiproliferative Effects ◽  
Metastatic Cell Line ◽  
Anti Cancer ◽  
Mcf 7

In this study, a new series of N-alkyl-3,6-dibromocarbazole and N-alkyl-5-bromoindole derivatives have been synthesized and evaluated in vitro as anti-cancer and anti-migration agents. Cytotoxic and anti-migratory effects of these compounds were evaluated in MCF-7 and MDA-MB-231 breast cancer cell lines and an insight on the structure-activity relationship was developed. Preliminary investigations of their anti-cancer activity demonstrated that several compounds have moderate antiproliferative effects on cancer cell lines with GI50 values in the range of 4.7–32.2 µM. Moreover, carbazole derivatives 10, 14, 15, 23, and 24 inhibit migration activity of metastatic cell line MDA-MB-231 in the range of 18–20%. The effect of compounds 10, 14, and 15 in extension of invadopodia and filopodia was evaluated by fluorescence microscopy and results demonstrated a reduction in actin-based cell extensions by compounds 10 and 15.

scholarly journals Evaluation of Anti-cancer and Pro-apoptotic Activities of Iranian Green Tea Extract Against A549,PC3, and MCF-7 Cancer Cell Lines

2019 ◽  
Vol 4 (3) ◽  
pp. 113-118
Author(s):  
Fatemeh Safari ◽  
Masomeh Rabieepor ◽  
Fatemeh Jamalomidi ◽  
Zahra Baghaeifar ◽  
Leila Khodaei
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Green Tea ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Apoptotic Protein ◽  
Anti Cancer ◽  
Tea Extract ◽  
Anti Tumor Activity ◽  
Mcf 7

Introduction: Green tea contains active polyphenols including catechins. The goal of the current study was to evaluate anti-cancer effects of Iranian green tea extract (IGTE) on 3 human cancer cell lines including A549, PC3, and MCF-7. Methods: First, Camellia sinensis was obtained from Lahijan, a city in the north of Iran and then IGTE was prepared. Next, catechins of IGTE were determined using high-performance liquid chromatography (HPLC). Finally, the cell viability of different cancer cells was evaluated by treatment with IGTE at concentration between 100 and 1000 µg/mL for 72 hours using MTT assay. Cell death of treated cancer cells was assessed by DAPI staining and RT-PCR method. Results: Our results demonstrated the potential anti-tumor activity of IGTE on MCF-7 cells (IC50= 400 µM), A549 cells (IC50= 500 µM), and PC3 cells (IC50= 600 µM), respectively. Chromatin damages within the nucleus of the treated cancer cells were shown. In addition, we found that IGTE induced apoptosis by up-regulation of Bax (a pro-apoptotic protein) and down-regulation of Bcl2 (an anti-apoptotic protein). Conclusion: Herein, we showed that IGTE is a potent natural product with anti-tumor activity on breast, lung, and prostate cancer cells. The efficacy of current therapies against cancer is limited by a range of adverse effects, toxicity, and drug resistance; therefore, new therapeutic strategies and more effective agents, particularly with natural origin, are desired and green tea may be a potent candidate in the field of cancer therapy

scholarly journals Diorganotin Complexes of a Thiosemicarbazone, Synthesis: Properties, X-Ray Crystal Structure, and Antiproliferative Activity of Diorganotin Complexes

2010 ◽  
Vol 2010 ◽  
pp. 1-9 ◽  
Author(s):  
Joanna Wiecek ◽  
Dimitra Kovala-Demertzi ◽  
Zbigniew Ciunik ◽  
Maria Zervou ◽  
Mavroudis A. Demertzis
Keyword(s):  
Hydrogen Bonds ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Bladder Cancer Cell Line ◽  
Intermolecular Hydrogen Bonds ◽  
X Ray ◽  
Mcf 7

The synthesis and spectral characterization of novel diorganotin complexes with 3-hydroxypyridine-2-carbaldehyde thiosemicarbazone,(1), [(L)](2), [(L)](3), and [(L)](4)are reported. The single-crystal X-ray structure of complex [(L)(DMSO)](5)shows that the ligand is doubly deprotonated and is coordinated as tridentate ligand. The six coordination number is completed by two carbon atoms of phenyl groups. There are two similar monomers5a(Sn1) and5b(Sn51) in the asymmetric unit. The monomers5aand5bare linked through intermolecular hydrogen bonds of N–H–O and C–H–S type. , intermolecular interactions, intra- and intermolecular hydrogen bonds stabilize this structure and leads to aggregation and a supramolecular assembly. The IR and NMR (, and ) spectroscopic data of the complexes are reported. The in vitro cytotoxic activity has been evaluated against the cells of three human cancer cell lines: MCF-7 (human breast cancer cell line), T-24 (bladder cancer cell line), A-549 (nonsmall cell lung carcinoma) and a mouse L-929 (a fibroblast-like cell line cloned from strain L). Compounds1,3, and4were found active against all four cell lines. Selectivity was observed for complexes3and4which were found especially active against MCF-7 and T-24 cancer cell lines.

scholarly journals Synthesis and Cytotoxic Evaluation of 3-(4-Fluorophenyl)-4,5-dihydro-5-(3,4,5-trimethoxy/4-nitrophenyl)-N-(substituted-phenyl)pyrazole-1-carboxamide Analogues

SynOpen ◽  
2018 ◽  
Vol 02 (02) ◽  
pp. 0114-0121
Author(s):  
Mohamed Ahsan ◽  
Bhawani Kumawat ◽  
Sonu Kumawat ◽  
Piush Sharma ◽  
Mohammad Bakht ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Srb Assay ◽  
Standard Drug ◽  
Sulforhodamine B ◽  
Structure Activity ◽  
Mcf 7

A novel series of 3-(4-fluorophenyl)-4,5-dihydro-5-(3,4,5-trimethoxy/4-nitro phenyl)-N-(substituted-phenyl)pyrazole-1-carboxamide analogues 4a–n was synthesized in two steps from 4-fluoroacetophenone. The pyrazoline analogues were evaluated for cytotoxicity against two breast cancer cell lines (MCF-7 and MBA-MD-231) by the sulforhodamine B (SRB) assay. N-(4-Chlorophenyl)-3-(4-fluorophenyl)-5-(4-nitrophenyl)-4,5-dihydro-1H-pyrazole-1-carboxamide (4b) showed the most promising cytotoxicity among the series, with GI50 <0.1 and 45.8 μM against the cancer cell lines, MCF-7 and MDA-MB-231, respectively. The anticancer activity of 4b was found to be comparable to that of the standard drug adriamycin (GI50 <0.1) against the MCF-7 cancer cell line. Structure activity relationships (SAR) are also considered.

Anticancer Evaluation of Novel Quinazolinone Acetamides: Synthesis and Characterization

2021 ◽  
Vol 21 ◽  
Author(s):  
Farhana Hakima ◽  
Roshan Salfi ◽  
Darna Bhikshapathi ◽  
Abdullah Khan
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Mouse Skin ◽  
Cancer Cell Lines ◽  
Chemotherapeutic Agents ◽  
Standard Drug ◽  
Anti Cancer ◽  
Normal Human ◽  
Cancer Activity ◽  
Mcf 7

Background: According to the global cancer report of 2019, the burden of cancer will exceed more than 18 million becoming one of the major causes of global mortality rate. There is a pressing need to establish novel drug candidates for cancer treatment, though many anticancer agents are available in the market owing to their adverse effects. In recent years, quinazoline and its derivatives have been considered as a novel class of cancer chemotherapeutic agents that show promising activity against different tumors. Objective: The objective of this study is to evaluate the anti-cancer potential of the novel class of quinazoline tethered acetamide derivatives against six different cancer cell lines. Method: A novel series of various substituted quinazolinone acetamides were synthesized through a feasible scheme. The synthetic scheme involves the conversion of benzoxazinone (from anthranilic acid and benzoyl chloride) intermediate to 3-amino quinazoline-4-one which is further converted to the final amide by tethering with the propionyl chloride employing Schotten-Baumann Reaction conditions. All the synthesized derivatives characterized by IR, 1HNMR and MASS spectral methods and anti-cancer activity evaluated by employing MTT assay for six cancer cell lines and one normal human cell line. Results: All the synthesized compounds were screened for anti-cancer activity against six cancer cell lines, including A 549 (lung), DU 145 (prostate), HT 29 (colon), MCF-7 (breast), SiHA (cervical), B16F10 (mouse skin melanoma) and one normal human fibroblast cell lines. All the compounds displayed a decent cytotoxicity profile when compared with the standard drug, doxorubicin. Among the synthesized compounds (5a to 5n) tested, two compounds, 5f and 5g have demonstrated excellent cytotoxicity against SiHA and MCF-7 cancer cell lines. Conclusion: Comparatively, most of the compounds displayed decent cytotoxicity potential relative to the standard drug, doxorubicin. Further investigations are needed to establish the detailed mechanism of action of the developed novel quinazolinone acetamides.

Design, Synthesis and In vitro Cytotoxic Evaluation of Novel Hybrids of Artemisinin and Quinazolinone

2021 ◽  
Vol 18 ◽  
Author(s):  
Tran Khac Vu ◽  
Bach Xuan Nguyen ◽  
Linh Nguyen Pham Duy ◽  
Thuc Bao Nguyen Truong ◽  
Anh Tuan Phung ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Human Cancer Cell ◽  
Human Cancer Cell Lines ◽  
Anti Cancer ◽  
Ic50 Values ◽  
Mcf 7

Background: In this study, two novel hybrid series of artemisinin and quinazolinones were synthesized and evaluated in vitro cytotoxicity against two human cancer cell lines, including SKLu-1 (lung cancer), MCF- 7 (breast cancer). The bio-assay results indicated that most of the target compounds exhibited cytotoxic activities against both human cancer cell lines tested, and seemed to be more cytotoxic toward the breast (MCF-7) cancer cells than lung (SKLu-1) cancer cells. Among the synthesized artemisinin hybrids, the compound 13d containing a quinazolinone conjugated system exhibited the most potent cytotoxicity against the SKLu-1 and MCF-7 cell lines with IC50 values of 1.62 and 0.77 µM, respectively. Objective: This study aims at developing novel hybrids of artemisinin and quinazolinones as anti-cancer agents. Method: A series of novel hybrids were designed, synthesized and evaluated for cytotoxicity against two human cancer cell lines, including SKLu-1 and MCF-7 using SRB method. Results : All thirteen hybrids of artemisinin with quinazolinone exhibited cytotoxic activity against two tested cancer cell lines, in which the compound 13d exhibited the most potent cytotoxicity against the SKLu-1 and MCF-7 cell lines with IC50 values of 1.62 and 0.77 µM, respectively. Conclusion: The research results suggest that some compounds could be considered as leads for future design of hybrids and have the potential for further studies in the field of anti-cancer agent development.

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