scholarly journals Combining Stem Cells and Biomaterial Scaffolds for Constructing Tissues and Cell Delivery

StemJournal ◽  
2019 ◽  
Vol 1 (1) ◽  
pp. 1-25 ◽  
Author(s):  
Stephanie M. Willerth ◽  
Shelly E. Sakiyama-Elbert
Keyword(s):  
Stem Cells ◽  
Cell Delivery ◽  
Biomaterial Scaffolds

scholarly journals Cancer Stem Cell Microenvironment Models with Biomaterial Scaffolds In Vitro

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 45
Author(s):  
Ghmkin Hassan ◽  
Said M. Afify ◽  
Shiro Kitano ◽  
Akimasa Seno ◽  
Hiroko Ishii ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cancer Biology ◽  
Advanced Technology ◽  
Cancer Microenvironment ◽  
Critical Factors ◽  
Self Renewal ◽  
Cell Microenvironment ◽  
Biological Phenomena ◽  
Biomaterial Scaffolds

Defined by its potential for self-renewal, differentiation and tumorigenicity, cancer stem cells (CSCs) are considered responsible for drug resistance and relapse. To understand the behavior of CSC, the effects of the microenvironment in each tissue are a matter of great concerns for scientists in cancer biology. However, there are many complicated obstacles in the mimicking the microenvironment of CSCs even with current advanced technology. In this context, novel biomaterials have widely been assessed as in vitro platforms for their ability to mimic cancer microenvironment. These efforts should be successful to identify and characterize various CSCs specific in each type of cancer. Therefore, extracellular matrix scaffolds made of biomaterial will modulate the interactions and facilitate the investigation of CSC associated with biological phenomena simplifying the complexity of the microenvironment. In this review, we summarize latest advances in biomaterial scaffolds, which are exploited to mimic CSC microenvironment, and their chemical and biological requirements with discussion. The discussion includes the possible effects on both cells in tumors and microenvironment to propose what the critical factors are in controlling the CSC microenvironment focusing the future investigation. Our insights on their availability in drug screening will also follow the discussion.

Development of a cell delivery system using alginate microbeads for tissue regeneration

2016 ◽  
Vol 4 (20) ◽  
pp. 3515-3525 ◽  
Author(s):  
Shirae K. Leslie ◽  
Anthony M. Nicolini ◽  
Gobalakrishnan Sundaresan ◽  
Jamal Zweit ◽  
Barbara D. Boyan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Delivery System ◽  
Tissue Regeneration ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Viable Cells ◽  
A Cell

Alginate microbeads incorporating adipose-derived stem cells (ASCs) have potential for delivering viable cells capable of facilitating tissue regeneration.

scholarly journals Multi-modal imaging probe for assessing the efficiency of stem cell delivery to orthotopic breast tumours

Nanoscale ◽  
2020 ◽  
Vol 12 (31) ◽  
pp. 16570-16585 ◽  
Author(s):  
May Zaw Thin ◽  
Helen Allan ◽  
Robin Bofinger ◽  
Tomas D. Kostelec ◽  
Simon Guillaume ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Delivery ◽  
Adipose Derived Stem Cells ◽  
Breast Tumours ◽  
Imaging Probe ◽  
Stem Cell Delivery

Illustration of adipose-derived stem cells with tri-modal imaging capabilities for evaluating the efficiency of cell delivery to tumours.

scholarly journals Placenta Derived Mesenchymal Stem Cells Hosted on RKKP Glass-Ceramic: A Tissue Engineering Strategy for Bone Regenerative Medicine Applications

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Mario Ledda ◽  
Marco Fosca ◽  
Angela De Bonis ◽  
Mariangela Curcio ◽  
Roberto Teghil ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Regenerative Medicine ◽  
Glass Ceramic ◽  
Phase Distribution ◽  
Sol Gel ◽  
Pcr Analysis ◽  
Cycle Phase ◽  
Cell Delivery ◽  
Limiting Factor

In tissue engineering protocols, the survival of transplanted stem cells is a limiting factor that could be overcome using a cell delivery matrix able to support cell proliferation and differentiation. With this aim, we studied the cell-friendly and biocompatible behavior of RKKP glass-ceramic coated Titanium (Ti) surface seeded with human amniotic mesenchymal stromal cells (hAMSCs) from placenta. The sol-gel synthesis procedure was used to prepare the RKKP glass-ceramic material, which was then deposited onto the Ti surface by Pulsed Laser Deposition method. The cell metabolic activity and proliferation rate, the cytoskeletal actin organization, and the cell cycle phase distribution in hAMSCs seeded on the RKKP coated Ti surface revealed no significant differences when compared to the cells grown on the treated plastic Petri dish. The health of of hAMSCs was also analysed studying the mRNA expressions of MSC key genes and the osteogenic commitment capability using qRT-PCR analysis which resulted in being unchanged in both substrates. In this study, the combination of the hAMSCs’ properties together with the bioactive characteristics of RKKP glass-ceramics was investigated and the results obtained indicate its possible use as a new and interesting cell delivery system for bone tissue engineering and regenerative medicine applications.

scholarly journals Intracarotid Injection of Fluorescence Activated Cell-Sorted CD49d-Positive Neural Stem Cells Improves Targeted Cell Delivery and Behavior After Stroke in a Mouse Stroke Model

Stroke ◽  
2008 ◽  
Vol 39 (4) ◽  
pp. 1300-1306 ◽  
Author(s):  
Raphael Guzman ◽  
Alejandro De Los Angeles ◽  
Samuel Cheshier ◽  
Raymond Choi ◽  
Stanley Hoang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Cell Delivery ◽  
Stroke Model ◽  
And Behavior

Stem cell–mediated regeneration of the intervertebral disc: cellular and molecular challenges

2008 ◽  
Vol 24 (3-4) ◽  
pp. E21 ◽  
Author(s):  
Rahul Jandial ◽  
Henry E. Aryan ◽  
John Park ◽  
William T. Taylor ◽  
Evan Y. Snyder
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Back Pain ◽  
Intervertebral Disc ◽  
Great Promise ◽  
Cell Delivery ◽  
Hostile Environment ◽  
Cell Type ◽  
Scientific Communities ◽  
Therapeutic Implications

✓ Regenerative medicine and stem cells hold great promise for intervertebral disc (IVD) disease. The therapeutic implications of utilizing stem cells to repair degenerated discs and treat back pain are highly anticipated by both the clinical and scientific communities. Although the avascular environment of the IVD poses a challenge for stem cell–mediated regeneration, neuroprogenitor cells have been discovered within degenerated discs, allowing scientists to revisit the hostile environment of the IVD as a target for stem cell therapy. Issues now under investigation include the timing of cell delivery and manipulation of stem cells to make them more efficient and adaptive in the IVD niche. This review covers the mechanisms underlying disc degeneration as well as the molecular and cellular challenges involved in directing stem cells to the desired cell type for intradiscal transplantation.

Human amnion as a novel cell delivery vehicle for chondrogenic mesenchymal stem cells

2009 ◽  
Vol 12 (1) ◽  
pp. 59-70 ◽  
Author(s):  
Sik-Loo Tan ◽  
Sofiah Sulaiman ◽  
Belinda Pingguan-Murphy ◽  
L. Selvaratnam ◽  
Cheh-Chin Tai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Delivery ◽  
Delivery Vehicle ◽  
Human Amnion

Engineered Cell Sheets for the Effective Delivery of Adipose-Derived Stem Cells for Tendon-to-Bone Healing

2020 ◽  
Vol 48 (13) ◽  
pp. 3347-3358
Author(s):  
Myung Jin Shin ◽  
In Kyong Shim ◽  
Dong Min Kim ◽  
Jae Hee Choi ◽  
Yu Na Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Bone Healing ◽  
Cell Sheet ◽  
The Other ◽  
Cell Delivery ◽  
Micro Ct ◽  
Stem Cell Delivery ◽  
Effective Delivery ◽  
Repair Group

Background: Efforts are being made to treat rotator cuff tears (RCTs) that exhibit poor healing and high retear rates. Tendon-to-bone healing using mesenchymal stem cells is being explored, but research is needed to establish effective delivery options. Purpose: To evaluate the effects of an adipose-derived stem cell (ADSC) sheet on mesenchymal stem cell delivery for tendon-to-bone healing of a chronic RCT in rats and to demonstrate that ADSC sheets enhance tendon-to-bone healing. Study Design: Controlled laboratory study. Methods: Mesenchymal stem cells were obtained from rat adipose tissue, and a cell sheet was prepared using a temperature-responsive dish. To evaluate the efficacy of stem cells produced in a sheet for the lesion, the experiment was conducted with 3 groups: repair group, cell sheet transplantation after repair group, and cell sheet–only group. Histological, biomechanical, and micro–computed tomography (micro-CT) results were compared among the groups. Results: Hematoxylin and eosin staining for histomorphological analysis revealed that the cell sheet transplantation after repair group (5.75 ± 0.95) showed statistically significant higher scores than the repair (2.75 ± 0.50) and cell sheet–only (3.25 ± 0.50) groups ( P < .001). On safranin O staining, the cell sheet transplantation after repair group (0.51 ± 0.04 mm2) had a larger fibrocartilage area than the repair (0.31 ± 0.06 mm2) and cell sheet–only (0.32 ± 0.03 mm2) groups ( P = .001). On micro-CT, bone volume/total volume values were significantly higher in the cell sheet transplantation after repair group (23.98% ± 1.75%) than in the other groups ( P < .039); there was no significant difference in the other values. On the biomechanical test, the cell sheet transplantation after repair group (4 weeks after repair) showed significantly higher results than the other groups ( P < .005). Conclusion: Our study shows that engineered stem cells are a clinically feasible stem cell delivery tool for rotator cuff repair. Clinical Relevance: This laboratory study provides evidence that ADSCs are effective in repairing RCTs, which are common sports injuries.

Multi-modal Cell Labelling for Quantification and Optimization of Stem Cell Delivery to Orthotopic Breast Tumors

2019 ◽  
Author(s):  
May Zaw Thin ◽  
Robin Bofinger ◽  
Tomas D. Kostelec ◽  
John Connell ◽  
P. Stephen Patrick ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Viability ◽  
Photon Emission ◽  
Breast Tumors ◽  
Whole Body ◽  
Cell Delivery ◽  
Cell Labelling ◽  
Cell Localization ◽  
Stem Cell Delivery

Stem cells have been utilized as anti-cancer agents due to their ability to home to and integrate within tumors. Methods to augment stem cell homing to tumors are being investigated with the goal of enhancing treatment efficacy. However, it is currently not possible to evaluate both cell localization and cell viability after engraftment, hindering optimization of therapy. In this study, luciferase expressing human adipocyte derived stem cells (ADSCs) were labelled with superparamagnetic iron oxide nanoparticles (SPIONs) conjugated to Indium-111 to produce cells with tri-modal imaging capabilities. ADSCs were administered intravenously (IV) or intracardially (IC) to mice bearing 4T1 orthotopic breast tumors. Cell fate was monitored serially using bioluminescence imaging (BLI) as a measure of cell viability, magnetic resonance imaging (MRI) for cell localization and single photon emission computer tomography (SPECT) for cell quantification. BLI/MRI/SPECT imaging revealed differences in whole body cell distribution between injection routes. BLI showed the presence of viable ADSCs within tumors as early as 1-hour post IC injection compared to 3 days post IV injection. SPECT data showed the percentage of ADSCs within tumors to be 2-fold higher after IC than IV at 5-hour post injection. Whereas, MRI confirmed the localization of SPION labelled cells in tumors after IC injection but not IV. Finally, histological analysis was used to validate engraftment of ADSC within tumor tissue. These findings demonstrate that multi-modal imaging can be used to evaluate the efficiency of stem cell delivery to tumors and that IC cell administration is more effective for tumor targeting.<br>

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