scholarly journals CORRELATION BETWEEN ENROVIRUS INFECTION AND ACUTE STROKE ON THE GROUND OF VIROLOGICAL, MOLECULAR GENETIC AND SEROLOGICAL METHODS OF DIAGNOSIS

2018 ◽  
Vol 13 (3-4) ◽  
pp. 38-45
Author(s):  
N.G. Andriushkova ◽  
N.S. Turchina ◽  
V.V. Melnуk ◽  
L.V. Dolinchuk ◽  
V.A. Poniatovskyi ◽  
...  
Keyword(s):  
Blood Serum ◽  
Acute Stroke ◽  
Main Group ◽  
Trigger Factor ◽  
Vascular Pathology ◽  
Enzyme Linked Immunosorbent Assay ◽  
Enterovirus Infection ◽  
Serum Samples ◽  
Isolation And Identification ◽  
Ig G

Relevance. Numerous virological studies prove the importance of enteroviruses in human somatic pathology. However, the etiopathogenetic role of enterovirus infection in patients with acute cerebrovascular disorder (GVMK) is not sufficiently highlighted. Objective: to establish the value of enterovirus infection as a trigger factor in the pathogenesis of acute stroke. Materials and methods. The pear blood serum of 72 patients with acute stroke (main group) and 35 patients with neurological pathology without vascular pathology (group of comparison) were screened for presence of enteroviruses using the virological method, detection of enterovirus genomes using a polymerase chain reaction and the presence of specific Ig M and Ig G to enteroviruses in the enzyme-linked immunosorbent assay (ELISA). Results. The enterovirus genomes were isolated from blood serum in 23,6±5,9 % of patients with acute stroke, that is significantly higher than in patients of the comparison group – 2,9±2,8 % (p <0,05). The enteroviruses were isolated in 11 cases of 17 PCR-positive blood serum samples of the main group. These viruses were identified as Coxsackie B viruses (serotypes 2, 3, 4) and ECHO viruses (serotypes 6, 9, 27 (two strains), 29), three strains of viruses could not be identified. The presence of specific Ig M and Ig G in blood serum of 4 patients with HPMC, as well as enterovirus genomes, has been established. It suggest that they have a recent enterovirus infection, or can indicate a recent enterovirus infection or exacerbation of chronic enterovirus infection. Only specific Ig G in the absence of Ig M were detected in blood serum of 4 PCR positive patients, that can indicate chronic enterovirus infection. Only Ig M in the absence of Ig G was detected in blood serum of 6 PCR-positive patients, that can indicate acute enterovirus infection. No Ig M or Ig G in serum from three PCR-positive patients were detected, possibly due to the presence of latent enterovirus infection. Conclusions. Acute and chronic persistent enterovirus infection plays a possible trigger role in the development of acute stroke.  The combination of PCR to detect genomes of enteroviruses, virological for the isolation and identification of viruses, and ELISA for the detection of specific Ig M and Ig G to enteroviruses should be recommended for the diagnosis of persistent enterovirus infection in patients with acute stroke.

scholarly journals Development of the ELISA Test System for Quantitative Determination of IgG to the Varizella zoster virus in Human Serum and Assessment of its Diagnostic Efficiency

2022 ◽  
Vol 20 (6) ◽  
pp. 72-80
Author(s):  
L. N. Lukhverchyk ◽  
G. L. Alatortseva ◽  
L. N. Nesterenko ◽  
V. Y. Kabargina ◽  
V. V. Dotsenko ◽  
...  
Keyword(s):  
Herpes Zoster ◽  
Blood Serum ◽  
Human Serum ◽  
Immunosorbent Assay ◽  
Test System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diagnostic Efficiency ◽  
Functional Characteristics ◽  
Serum Samples ◽  
Population Immunity

Relevance. The introduction of Varicella vaccine prophylaxis explains the need to develop a methodology for monitoring the vaccination effectiveness and the intensity of population immunity. This problem can be solved using quantitative immunoassay methods. Aim. Development of an enzyme-linked immunosorbent assay for the concentration of class G immunoglobulins (AB) to Varicella zoster virus (VZV) determining and assessing its functional characteristics and diagnostic efficiency. Materials and methods. Recombinant antigen GE VZV. WHO International Standard for Antibodies to VZV W1044. Blood serum samples from healthy people and patients with Chickenpox and Herpes zoster, blood serum samples containing IgG antibodies to herpes simplex viruses of the first and second types, cytomegalovirus, Epstein-Barr virus. Anti-VZV ELISA (IgG) reagent kit (Euroimmun, Germany). Indirect enzyme-linked immunosorbent assay. Immunization of animals with recombinant antigen GE, isolation, and purification of specific antibodies. Conjugation of monoclonal antibodies to human IgG with antibodies to antigen GE and with horseradish peroxidase. Results. An enzyme-linked immunosorbent assay in «an indirect» format has been developed to determine the specific antibodies to VZV concentration (IU/ml) in human serum/plasma. An artificial calibrator for determining the concentration of AB-VZV had been synthesized and standardized according to the International WHO-standard W1044. The main functional characteristics of the developed enzyme-linked immunosorbent assay are determined in accordance with GOST 51352-2013. The diagnostic kit was tested on blood serum samples from children with chickenpox (n = 43), adults with Herpes zoster (n = 158), healthy individuals (n = 781). The diagnostic sensitivity of the test system was 85%, the diagnostic specificity was 87% according to the ROC analysis. The absence of cross-reactivity of the test system was shown on samples with serological markers of other herpesvirus infections (n = 94). Comparative trials of the developed test system and its commercial analog, the Anti-VZV ELISA (IgG) reagent kit, did not reveal statistically significant differences between their functional characteristics. Conclusions. The developed test system for determining of the AB-VZV concentration in human serum/plasma in terms of its functional characteristics meets the GOST requirements, is characterized by high diagnostic efficiency, can be used to monitor the effectiveness of vaccine prophylaxis and strength of population immunity, as well as to assess the immune response in chickenpox and Herpes zoster.

scholarly journals A study of Neospora caninum antibody seroprevalence ın dairy cows in Turkey

2020 ◽  
Vol 71 (1) ◽  
pp. 2018
Author(s):  
S. KASAP ◽  
S. ERTUNC ◽  
E. M. TEMIZEL ◽  
S. SENTURK
Keyword(s):  
Blood Serum ◽  
Dairy Cows ◽  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Total Sample ◽  
Enzyme Linked Immunosorbent Assay ◽  
Marmara Region ◽  
Serum Samples ◽  
Caninum Antibody ◽  
Intracellular Protozoan Parasite

Neospora caninum is a intracellular protozoan parasite and is one of the major causes of repeated abortions, foetal malformations, pre-term deliveries, stillbirth and possible loss of milk yield in livestock. The presence of specific antibodies against N. caninum in the blood serum of dairy cows is investigated in the present study. A total of 184 blood serum samples of dairy cows were examined in Bursa province in the Marmara Region. N. caninum antibodies were measured using an indirect enzyme-linked immunosorbent assay (ELISA) (The Svanovir Neospora-Ab ELISA). From the total sample, antibodies to N. caninum were detected in 62 of the 184 examined cows (33.3%) and neurological findings were seen in a calf.

scholarly journals Conventional and molecular diagnosis of Campylobacteriosis associated with bovine abortion

2021 ◽  
Vol 52 (2) ◽  
Author(s):  
Aiswarya N ◽  
Binu K. Mani ◽  
Surya Sankar ◽  
Mini M ◽  
Unnikrishnan M. P.
Keyword(s):  
Control Method ◽  
Stomach Contents ◽  
Economic Loss ◽  
Clinical Samples ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Isolation And Identification ◽  
Livestock Sector ◽  
History Of ◽  
Tract Infections

Campylobacteriosis is responsible for genital tract infections of beef and dairy herds, causing a significant economic loss in livestock sector. Campylobacter foetus species is one of the important pathogens because of its potential impact in Veterinary and Human health. This study was designed to determine the regional incidence of C. foetus infection in Kerala, India by isolation, detection of C. foetus in clinical samples by Polymerase Chain Reaction (PCR), real time PCR (qPCR), and Enzyme Linked Immunosorbent Assay (ELISA) for the detection of C. foetus antibodies in sera of bovines with the history of abortion/infertility. Clinical samples (aborted materials (50), serum (50), Cervico-Vaginal Mucus (CVM) (30) and semen samples (30)) from a total of 160 cattle and buffaloes with the history of abortion and infertility were collected. Aborted materials including placenta, foetal membranes, liver, lungs and stomach contents of the aborted foetus, semen and CVM samples were processed and subjected to isolation and identification of Campylobacter foetus subsp. foetus (Cff) and Campylobacter foetus subsp. venerealis (Cfv) and molecular confirmation by PCR and qPCR respectively. Serum samples from aborted dams were tested using indirect ELISA. All the suspected clinical samples were found negative for Cff and Cfv on both culturing and PCR. All the serum samples tested were negative by ELISA as well. Conclusively the study indicated the infection of C. foetus spp. responsible for abortion in bovine are rare in the location where the study was conducted, which might be due to insignificant endemic levels. As per the breeding policy, only artificial insemination is practiced in Kerala in bovines, which is often considered as a simple control method for Bovine Genital Campylobacteriosis (BGC) and might be one of the factors that prevented extensive spread of C. foetus spp. infection.

Prevalence of antibodies to Bovine Viral Diarrhea Virus (BVDV) in blood and milk serum in dairy cattle in Kars district of Turkey

2016 ◽  
Author(s):  
Volkan Yilmaz
Keyword(s):  
Blood Serum ◽  
Bovine Viral Diarrhea Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Viral Diarrhea ◽  
Dairy Herds ◽  
Serum Samples ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Milk Serum ◽  
Viral Diarrhea Virus

The purpose of this study was to detect of antibodies against Bovine Viral Diarrhea Virus (BVDV) in blood and milk serum samples. With this aim, a total of 192 blood and milk samples were collected from unvaccinated Holstein cows in Kars district of Turkey. Blood and milk serum samples were tested to determine the presence of antibodies against BVDV by commercial indirect enzyme-linked immunosorbent assay (ELISA). In this study, while 172 of blood serum (89.58%) were found to be positive for the presence of BVDV antibodies, 161 of milk serum samples (83.85%) were positive. In addition, 150 (78.12%) both blood and milk serum samples of same cattle were positive for BVDV antibodies. Only 22 (11.45%) blood serum of cattle was detected positive for BVDV, while only 11 (5.73%) milk serum was seropositive. Data obtained from the study showed the presence of BVDV infection in dairy herds in the Kars region and demonstrate that blood serum and milk serum samples might be consistent with one another in the determination of BVDV seroprevalence.

scholarly journals Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays

2018 ◽  
Vol 69 (3) ◽  
pp. 1088
Author(s):  
A. GAVRILOVIĆ ◽  
P. GAVRILOVIĆ ◽  
S. RADOJIČIĆ ◽  
D. KRNJAIĆ
Keyword(s):  
Blood Serum ◽  
Immunosorbent Assay ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Response ◽  
Rapid Diagnostics ◽  
Serum Samples ◽  
Perfect Agreement ◽  
First Time

Bluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohen’s kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.

scholarly journals Evaluation of epizootic situation on arthritis-encephalitis of goats in novosibirsk region

2020 ◽  
Vol 49 (6) ◽  
pp. 104-108
Author(s):  
I. S. Onishchenko ◽  
I. N. Pen’kova ◽  
N. Yu. Balybina ◽  
M. A Leonova ◽  
V. Yu. Koptev
Keyword(s):  
Blood Serum ◽  
High Titer ◽  
Encephalitis Virus ◽  
Diagnostic Methods ◽  
Farm Households ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Novosibirsk Region ◽  
Private Farm

The analysis of the epizootic situation for viral arthritis-encephalitis of goats in the territory of Novosibirsk region was carried out. No specifi  c prophylaxis for this disease has been developed, so the earliest diagnostic methods, as well as the study of its epizootology, are relevant. The studies were conducted in 2019. To study the distribution of goats that are positively responsive to viral arthritis-encephalitis, 198 blood serum samples were taken from goats of various genders, breeds and ages in private farm households and farm enterprises located on the territory of Novosibirsky, Iskitimsky, Ordynsky, Kochenevsky, Moshkovsky and Maslyaninsky districts of Novosibirsk region. In order to study the presence of antibodies to goat arthrit-isencephalitis virus in diagnostic titers, an indirect enzyme-linked immunosorbent assay was used with the antibody detection kit against MVV / CAEV in goat serum (ID Screen® MVV / CAEV Indirect Screening test). Of the 198 animals examined, 86 were found to have diagnostically signifi  cant titers of antibodies to the goat arthritis- encephalitis virus, which was 43.4% of the studied population. The result for two goats was uncertain, which amounted to 1%. The remaining animals (55.6%) had no antibodies to goat arthritis-encephalitis virus in their blood serum. The maximum number of positively reacting animals was noted in Novosibirsky district – 66.7%. The Maslyaninsky district was second according to the degree of virus carrying, whereby 47.5% of blood serum samples of goats showed a high titer of antibodies to the goat arthritis-encephalitis virus. The data obtained indicate that at least fi  ve districts of the Novosibirsk Region have foci of goat arthritis-encephalitis virus.

scholarly journals COMPARISON OF RESULTS OBTAINED BY ELISA AND NEUTRALIZATION TEST IN ASSESSING THE PROTECTION OF POPULATION FROM TICK-BORNE ENCEPHALITIS

2018 ◽  
Vol 63 (1) ◽  
pp. 36-40 ◽  
Author(s):  
L. L. Chernokhaeva ◽  
G. B. Maikova ◽  
Yu. V. Rogova ◽  
V. V. Romanenko ◽  
A. V. Ankudinova ◽  
...  
Keyword(s):  
Blood Serum ◽  
Neutralizing Antibodies ◽  
Neutralization Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Protection Level ◽  
Tick Borne Encephalitis ◽  
Inactivated Vaccines ◽  
Total Pool ◽  
The Difference

The enzyme-linked immunosorbent assay (ELISA) and the neutralization test (NT) are often used to determine the level of seropositive population and to evaluate the immunogenicity of vaccines. ELISA provides information on the total pool of antiviral antibodies, while NT allows the antiviral protection level of a person to be estimated. It is assumed that the 1:100 titer in ELISA and the 1:10 titer in NT are protective. Obviously, the ratio of the total pool and virus neutralizing antibodies can vary as a result of natural immunization or vaccination. In this study, two methods were used to study the blood serum samples taken in a group of inhabitants of the Sverdlovsk region aged from 1 to 60 years. The samples were collected before immunization and 30 days after two immunizations with inactivated vaccines against tick-borne encephalitis of different manufacturers. Immunizations were performed either according to a standard scheme (30-day interval between immunizations), or according to an emergency scheme (14-day interval). It was shown that the data on the presence of antiviral antibodies in protective titers obtained by ELISA and NT were consistent in more than 85% of cases. The discrepancies between the data are due, in the first place, to the difference in the sensitivities of the two methods. The proportion of seropositive people according to NT data is always greater than that according to the results of ELISA. Nevertheless, among 174 children, about 5% of recipients after a double immunization were seropositive according to ELISA, but did not have neutralizing antibodies in protective titers.

scholarly journals Serological Studied On Toxoplasma Gondii Infection in Sheep and Goats in Ismailia Province

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Toxoplasma Gondii ◽  
Blood Serum ◽  
Elisa Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Serum Samples ◽  
Sheep And Goats ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Blood serum samples from 100 sheep and 100 goats were collected and examined for Toxoplasma gondii antibodies by Enzyme Linked Immunosorbent Assay and Modified Agglutination Test. The seroprevalences of Toxoplasma gondii in sheep were 34% and 33% and in goats were 32%, 31% by ELISA and MAT respectively. The prevalence in the females of sheep and goats were higher than males. The seroprevalences were higher in adult animals than young in both sheep and goats. Using the MAT as a reference test, the sensitivity and specificity of the ELISA Test were 100% and 98.5% respectively

Serum Feline Pancreatic Lipase Immunoreactivity Concentration and Seroprevalences of Antibodies Against Toxoplasma Gondii and Bartonella Species in Client-Owned Cats

2009 ◽  
Vol 11 (8) ◽  
pp. 663-667 ◽  
Author(s):  
Danielle B. Bayliss ◽  
Jörg M. Steiner ◽  
Jan S. Sucholdolski ◽  
Steven V. Radecki ◽  
Melissa M. Brewer ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Pancreatic Lipase ◽  
Clinical Findings ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Bartonella Species ◽  
Serum Samples ◽  
Serological Tests ◽  
Ig G

Feline pancreatitis is a commonly suspected illness and it has been proposed that some cases of feline pancreatitis may be caused by infection with Toxoplasma gondii or Bartonella species. Feline pancreatic lipase immunoreactivity (fPLI) is a test performed on serum that is commonly combined with other clinical findings as an indirect aid in the diagnosis of pancreatitis. The purpose of this study was to determine if there are associations between fPLI concentration and the presence of serum antibodies against T gondii or Bartonella species. Serum samples from 458 cats, for which serum fPLI concentrations had already been determined, were assayed by enzyme-linked immunosorbent assay for the presence of T gondii immunoglobulin (Ig) G (IgG) and IgM antibodies, and Bartonella species IgG antibodies. The association between fPLI concentration and T gondii or Bartonella species antibodies was determined. No statistically significant association was found between fPLI concentration and T gondii or Bartonella species antibodies, suggesting that serological tests for the organisms are not useful in cases with increased fPLI concentration.

TBE in Sweden

2020 ◽  
Keyword(s):  
Genetic Characterization ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Second Phase ◽  
Serum Samples ◽  
Tick Borne Encephalitis ◽  
Specific Immunoglobulin ◽  
Tick Borne Encephalitis Virus ◽  
First Time

Tick-borne encephalitis virus (TBEV) was isolated for the first time in Sweden in 1958 (from ticks and from 1 tick-borne encephalitis [TBE] patient).1 In 2003, Haglund and colleagues reported the isolation and antigenic and genetic characterization of 14 TBEV strains from Swedish patients (samples collected 1991–1994).2 The first serum sample, from which TBEV was isolated, was obtained 2–10 days after onset of disease and found to be negative for anti-TBEV immunoglobulin M (IgM) by enzyme-linked immunosorbent assay (ELISA), whereas TBEV-specific IgM (and TBEV-specific immunoglobulin G/cerebrospinal fluid [IgG/CSF] activity) was demonstrated in later serum samples taken during the second phase of the disease.

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