scholarly journals Serological Studied On Toxoplasma Gondii Infection in Sheep and Goats in Ismailia Province

2018 ◽  
Vol 2 (2) ◽  
Keyword(s):  
Toxoplasma Gondii ◽  
Blood Serum ◽  
Elisa Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Reference Test ◽  
Serum Samples ◽  
Sheep And Goats ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Blood serum samples from 100 sheep and 100 goats were collected and examined for Toxoplasma gondii antibodies by Enzyme Linked Immunosorbent Assay and Modified Agglutination Test. The seroprevalences of Toxoplasma gondii in sheep were 34% and 33% and in goats were 32%, 31% by ELISA and MAT respectively. The prevalence in the females of sheep and goats were higher than males. The seroprevalences were higher in adult animals than young in both sheep and goats. Using the MAT as a reference test, the sensitivity and specificity of the ELISA Test were 100% and 98.5% respectively

scholarly journals Seroprevalence of Toxoplasma gondii infection in sheep in Inner Mongolia Province, China

Parasite ◽  
2020 ◽  
Vol 27 ◽  
pp. 11 ◽  
Author(s):  
Xinlei Yan ◽  
Wenying Han ◽  
Yang Wang ◽  
Hongbo Zhang ◽  
Zhihui Gao
Keyword(s):  
Toxoplasma Gondii ◽  
Inner Mongolia ◽  
Animal Husbandry ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Zoonotic Parasite ◽  
Health And Disease ◽  
Toxoplasma Gondii Infection ◽  
Almost All

Toxoplasma gondii is an important zoonotic parasite that can infect almost all warm-blooded animals, including humans, and infection may result in many adverse effects on animal husbandry production. Animal husbandry in Inner Mongolia is well developed, but data on T. gondii infection in sheep are lacking. In this study, we determined the seroprevalence and risk factors associated with the seroprevalence of T. gondii using an indirect enzyme-linked immunosorbent assay (ELISA) test. A total of 1853 serum samples were collected from 29 counties of Xilin Gol League (n = 624), Hohhot City (n = 225), Ordos City (n = 158), Wulanchabu City (n = 144), Bayan Nur City (n = 114) and Hulunbeir City (n = 588). The overall seroprevalence of T. gondii was 15.43%. Risk factor analysis showed that seroprevalence was higher in sheep ≥12 months of age (21.85%) than that in sheep <12 months of age (10.20%) (p < 0.01). Seroprevalence was higher in male sheep (18.76%) than females (12.80%) (p < 0.01). Barn-feeding sheep (23.13%) had higher prevalence than grazing sheep (10.94%) (p < 0.01). The seroprevalence was significantly different in different districts (p < 0.01). This study shows that sheep are exposed to T. gondii in Inner Mongolia, and provides a data reference for public health and disease control.

scholarly journals Potential linkage between Toxoplasma gondii Infection and physical education scores of College Students

2020 ◽  
Author(s):  
Zhijin Sheng ◽  
Yu Jin ◽  
Yinan Du ◽  
Xinlei Yan ◽  
Yong Yao
Keyword(s):  
College Students ◽  
Physical Education ◽  
Toxoplasma Gondii ◽  
Protozoan Parasite ◽  
Anhui Province ◽  
Behavioral Changes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
School Students ◽  
Toxoplasma Gondii Infection

ABSTRACTObjectiveToxoplasma gondii is a worldwide protozoan parasite that could infect virtually all warm-blooded animals, including humans. Our study aimed to investigate the prevalence of T. gondii infection in college students at Anhui province, China. Moreover, growing studies demonstrated the association between T. gondii infection and host behavioral changes. We also studied the linkage between T. gondii and scores of college students.Methods2704 serum samples of medical school students attending physical education lessons were collected from September 2017 to September 2019 and evaluated for T. gondii IgG antibodies using an enzyme-linked immunosorbent assay (ELISA). We also analysed PE scores of T. gondii infected students and T. gondii uninfected students.ResultsThe overall seroprevalence of T. gondii was 11.5%. The main risk factors related to T. gondii infections were cat in the household and gardening or agriculture activity. Furthermore, in basketball group and football group, scores of T. gondii seropositive students were significantly higher than that of seronegative students, while in other sports there is no difference between scores of T. gondii infected students and T. gondii uninfected students.ConclusionThis is the first report of T. gondii seroprevalence in college students in Anhui province, China.

scholarly journals Seroprevalence of Toxoplasma gondii and Encephalitozoon cuniculi among domestic rabbits in central China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 9 ◽  
Author(s):  
Shuai Wang ◽  
Zhijun Yao ◽  
Lingjuan Li ◽  
Yaoqian Pan ◽  
Pengju Li ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Central China ◽  
Enzyme Linked Immunosorbent Assay ◽  
Meat Production ◽  
Health Issues ◽  
Encephalitozoon Cuniculi ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Domestic Rabbits

Rabbits (Oryctolagus cuniculus) are frequently reared for meat production in China. The aim of this study was to assess the seroprevalence of Toxoplasma gondii and Encephalitozoon cuniculi, and risk factors of infection in domestic rabbits raised in Henan province, central China. 1,213 serum samples of domestic rabbits were collected and tested for anti-T. gondii and anti-E. cuniculi antibodies using a modified agglutination test (MAT) and an enzyme-linked immunosorbent assay (ELISA), respectively. The serum positive rates of T. gondii and E. cuniculi were 128/1,213 (10.55%) and 235/1,213 (19.37%), respectively. Co-infection of T. gondii and E. cuniculi was demonstrated in 84 specimens; 44 rabbits were seropositive for T. gondii alone, while 151 rabbits were seropositive for E. cuniculi alone. The main risk factors simultaneously associated with T. gondii and E. cuniculi infection were the age of the rabbit, the type of food, and the rabbit rearing system. Serum positive rates of T. gondii and E. cuniculi among domestic rabbits were high, indicating the possibility of public health issues.

Cross-sectional survey of Toxoplasma gondii infection in colony cats from urban Florence (Italy)

2010 ◽  
Vol 12 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Francesca Mancianti ◽  
Simona Nardoni ◽  
Gaetano Ariti ◽  
Dario Parlanti ◽  
Giovanna Giuliani ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Serum Samples ◽  
Cross Sectional Survey ◽  
Nested Polymerase Chain Reaction ◽  
Cross Sectional ◽  
Modified Agglutination Test ◽  
Faecal Samples ◽  
Key Species ◽  
Polymerase Chain ◽  
Toxoplasma Gondii Infection

Cats are the key species in the epidemiology of Toxoplasma gondii infection, even if the proportion of subjects excreting oocysts is low. The aim of the present paper was to obtain information about seroprevalence, oocyst shedding rate and presence of T gondii DNA in faeces collected from an urban population of colony cats in Florence (Tuscany). Fifty European shorthair feral cats were examined for anti- T gondii specific antibodies by a modified agglutination test (MAT), and for oocysts by microscopic examination and for faecal protozoal DNA, by means of a nested polymerase chain reaction (n-PCR) protocol. Twenty-two out of 50 serum samples (44%) were MAT positive. T gondii oocysts were not detected in any of the examined faecal samples. Eight out of 50 faecal specimens (16%) were n-PCR positive and sequencing of the bands was specific for T gondii. Detection by combination of the two methods was higher than single techniques and enhanced the detection of T gondii up to 48%. Our results suggest that the use of MAT plus PCR in faeces may be the best choice for diagnosis of feline toxoplasmosis. Further studies to ascertain the real infectivity of the copro-PCR positive subjects are required.

Seroprevalence and correlates of Toxoplasma gondii infection in dairy cattle in northwest China

2015 ◽  
Vol 60 (4) ◽  
Author(s):  
Qi-Dong Tan ◽  
Xiao-Yu Yang ◽  
Ming-Yang Yin ◽  
Ling-Ying Hu ◽  
Si-Yuan Qin ◽  
...  
Keyword(s):  
Risk Factors ◽  
Toxoplasma Gondii ◽  
Dairy Cattle ◽  
Epidemiological Data ◽  
Northwest China ◽  
Gansu Province ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Significant Difference ◽  
Toxoplasma Gondii Infection

AbstractPrevalence of antibodies to Toxoplasma gondii and risk factors with infection were assessed in dairy cattle from Gansu Province and Ningxia Hui Autonomous Region (NXHAR), northwest China. In total, 1657 serum samples were collected and assayed by the modified agglutination test. The overall seroprevalence was 4.83% at a 1:100 cut-off, with titers of 1:100 in 72, 1:200 in 4, 1:400 in 4. Among the risk factors examined, no statistically significant difference was observed between T. gondii seroprevalence and regions or age of dairy cattle in the logistic regression analysis (P>0.05) and left out of the final model. However, numbers of pregnancies of dairy cattle was considered as main risk factor associated with T. gondii infection. Dairy cattle in nulliparity group (8.89%) had 6 times (OR=6.31, 95% CI, 2.69-14.83, P<0.001) higher risk of being seropositive compared to dairy cattle in 3 or above 3 pregnancies group (1.52%), followed by 1 pregnancy group (4.27%) had nearly 3 times (OR=2.89, 95% CI, 1.11-7.52, P = 0.03) higher risk of being seropositive compared to dairy cattle in 3 or above 3 pregnancies group, although no statistical difference was found between 2 pregnancies group and 3 or above 3 pregnancies group (P = 0.70). The results of this survey indicated the presence of T. gondii infection in dairy cattle in Gansu Province and NXHAR, which enriches the epidemiological data of T. gondii infection in dairy cattle in China, and is helpful to strengthen prevention and control of T. gondii infection in dairy cattle in these two regions.

scholarly journals SURVEI SEROLOGIS TOXOPLASMOSIS PADA TERNAK SAPI DI KABUPATEN PRINGSEWU MENGGUNAKAN METODE TOXOPLASMA MODIFIED AGGLUTINATION TEST (To-MAT)

2019 ◽  
Vol 7 (3) ◽  
pp. 270
Author(s):  
Galang B. ◽  
Emantis R. ◽  
Gina Dania P. ◽  
Endah S.
Keyword(s):  
Toxoplasma Gondii ◽  
Agglutination Test ◽  
Test Method ◽  
Cattle Population ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

The cattle population in Lampung Province has a relatively high number when compared to several other provinces. Pringsewu is one of the districts in Lampung Province having the supplier potential of beef in Lampung. The higher the population and production, could cause a potential infection of toxoplasmosis to livestock and human. So far the prevalence of toxoplasmosis in Pringsewu District. This study was conducted to determine the level of toxoplasmosis infection in cattle in Pringsewu district. This study used the To-MAT (Toxoplasma Modified Agglutination Test) method to test Toxoplasma gondii infection in blood of cows samples. The result of the study found that at a chronic level there were 23/24 cows positively infected with T.gondii. Whereas in acute level there are 8/24 cows positively infected with T. gondii. In general, the prevalence of toxoplasmosis is 95.8%. Keywords : Toxoplasmosis, Cow, To-MAT (Toxoplasma Modified Agglutination Test) method, Infection

scholarly journals First report on seroprevalence and risk factors of Toxoplasma gondii infection in sheep and goats in North Lebanon

2019 ◽  
Vol 13 (09) ◽  
pp. 831-836 ◽  
Author(s):  
Dima El Safadi ◽  
Dany Abi Chahine ◽  
Alissar Al Tarraf ◽  
Omar Raii ◽  
Karim Mesto ◽  
...  
Keyword(s):  
Risk Factors ◽  
Risk Factor ◽  
Toxoplasma Gondii ◽  
Immunoglobulin G ◽  
Agglutination Test ◽  
Economic Losses ◽  
Transplacental Transmission ◽  
Igg Antibodies ◽  
Modified Agglutination Test ◽  
Toxoplasma Gondii Infection

Introduction: Toxoplasmosis is of dual importance in both public and veterinary health due to the respective risk of transplacental transmission in primo-infected pregnant women and economic losses caused by abortions in mammals. One of the main routes of Toxoplasma gondii transmission to humans is the consumption of raw or undercooked meats containing parasitic cysts. Here, we performed the first epidemiological study to determine the seroprevalence and the risk factors of toxoplasmosis in livestock in Lebanon. Methodology: Using a modified agglutination test with a cut-off of 1:40, we tested the positivity rate of Immunoglobulin G antibodies in the sera of 100 sheep and 80 goats collected from 18 different livestock farms located in North Lebanon between March and June 2018. Results: Anti-Toxoplasma gondii IgG antibodies were detected in 42% of sheep and 34% of goats. Adults (> 1 year) were significantly more infected by T. gondii than the lambs (< 1 year) in both species (p < 0.05). Conclusions: These findings indicated that food animals are highly exposed to T. gondii in Lebanon and could be potentially a major risk factor of T. gondii infection to humans. Consequently, national prophylactic strategies should be implemented to control and to prevent T. gondii transmission between animals and humans.

scholarly journals Seroprevalence of Toxoplasma gondii and Leishmania spp. in domestic donkeys from Portugal

2019 ◽  
Vol 28 (1) ◽  
pp. 172-176 ◽  
Author(s):  
Filipa Teixeira Rodrigues ◽  
Catarina Pereira ◽  
Jitender Prakash Dubey ◽  
Miguel Nóvoa ◽  
Miguel Quaresma ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Leishmania Infantum ◽  
Agglutination Test ◽  
Direct Agglutination Test ◽  
Serum Samples ◽  
Modified Agglutination Test ◽  
Leishmania Spp

Abstract Toxoplasma gondii and Leishmania infantum are zoonotic protozoal parasites. Serum samples were obtained from 186 donkeys (Equus africanus asinus) from Portugal and assessed for antibodies to T. gondii by the modified agglutination test (MAT). For titration of antibodies to Leishmania spp. the direct agglutination test was used (DAT). Eleven donkeys were seropositive for T. gondii with titres of 20 (n = 7), 80 (n = 2), 640 (n = 1) and ≥ 2560 (n = 1). One donkey was seropositive for Leishmania spp. (titre of 800). Donkeys in Portugal are exposed to and can be infected with T. gondii and Leishmania spp.

scholarly journals Seroepidemiology of Toxoplasma gondii infection in patients with liver disease in eastern China

2017 ◽  
Vol 145 (11) ◽  
pp. 2296-2302 ◽  
Author(s):  
A. L. TIAN ◽  
G. X. LI ◽  
H. M. ELSHEIKHA ◽  
D. S. GARDNER ◽  
X. Y. ZHANG ◽  
...  
Keyword(s):  
Risk Factors ◽  
Liver Disease ◽  
Toxoplasma Gondii ◽  
Eastern China ◽  
Multivariate Logistic Regression Analysis ◽  
Protozoan Parasite ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Toxoplasma Gondii Infection

SUMMARYThe role of the protozoan parasite Toxoplasma gondii in the pathogenesis of liver disease has recently gained much interest. The aim of this study was to determine the prevalence and risk factors associated with T. gondii infection in patients with liver disease from three cities in Shandong and Henan provinces, China. A case–control study was conducted from December 2014 to November 2015 and included 1142 patients with liver disease and 1142 healthy controls. Serum samples were collected from all individuals and were examined with enzyme-linked immunosorbent assay for the presence of anti-T. gondii IgG and IgM antibodies. Information on the demographics, clinical, and lifestyle characteristics of the participants was collected from the medical records and by the use of a questionnaire. The prevalence of anti-T. gondii IgG was 19·7% in patients with liver disease compared with 12·17% in the controls. Only 13 patients had anti-T. gondii IgM antibodies compared with 12 control individuals (1·14% vs. 1·05%, respectively). The highest seroprevalence was detected in patients with liver cancer (22·13%), followed by hepatitis patients (20·86%), liver cirrhosis patients (20·42%), and steatosis patients (20%). Multivariate logistic regression analysis indicated that consumption of raw meat (odds ratio (OR) = 1·32; 95% confidence interval (CI) 1·01–1·71; P = 0·03) and source of drinking water from wells (OR = 1·56; 95% CI 1·08–2·27; P = 0·01) were independent risk factors for T. gondii infection in liver disease patients. These findings indicate that T. gondii infection is more likely to be present in patients with liver disease. Therefore, efforts should be directed toward health education of populations at high risk of T. gondii infection and measures should be taken to protect vulnerable patients with liver disease.

scholarly journals Use of MAG1 Recombinant Antigen for Diagnosis of Toxoplasma gondii Infection in Humans

2007 ◽  
Vol 14 (3) ◽  
pp. 220-225 ◽  
Author(s):  
Lucyna Holec ◽  
Elżbieta Hiszczyńska-Sawicka ◽  
Artur Gąsior ◽  
Anna Brillowska-Dąbrowska ◽  
Józef Kur
Keyword(s):  
Toxoplasma Gondii ◽  
Diagnostic Tests ◽  
Expression System ◽  
Recombinant Antigen ◽  
Acute Stage ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Acute Toxoplasmosis ◽  
Toxoplasma Gondii Infection ◽  
Potential Use

ABSTRACT This paper describes the cloning, purification, and serological applications of matrix antigen MAG1 of Toxoplasma gondii. The expression system used allows the production of a large amount of T. gondii recombinant protein, which was assessed for its potential use in an enzyme-linked immunosorbent assay (ELISA) for detection of T. gondii infection in humans. Serum samples from 117 patients with different stages of infection, along with 10 serum samples from seronegative patients obtained for routine diagnostic tests, were used. The results were compared with those of an ELISA that uses a native T. gondii antigen extract. The MAG1 antigen detected antibodies more frequently from the acute stage (97.3%) than from the chronic stage (7.5%) of toxoplasmosis. Hence, this antigen may be used as a tool for detection of T. gondii immunoglobulin G antibodies in persons with acute toxoplasmosis.

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