scholarly journals CYP2E1 Gene

2020 ◽  
Author(s):  
Keyword(s):  
Cyp2e1 Gene

Role of the Genetic Polymorphisms in the 5'-Flanking Region for Transcriptional Regulation of the Human CYP2E1 Gene

2007 ◽  
Vol 31 (s1) ◽  
pp. S36-S42 ◽  
Author(s):  
Takayuki Uchimoto ◽  
Sakae Itoga ◽  
Masahiko Nezu ◽  
Masahiko Sunaga ◽  
Takeshi Tomonaga ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Genetic Polymorphisms ◽  
Cyp2e1 Gene ◽  
Flanking Region

scholarly journals CYP2E1-DEPENDENT VARIATIONS IN HEPATOCYTES DAMAGE DURING TREATMENT OF TUBERCULOSIS

2019 ◽  
Vol 16 (3) ◽  
pp. 20-26
Author(s):  
L.V. Natrus ◽  
L.V. Gayova ◽  
O.O. Gorkunenko ◽  
P.A. Chernovol ◽  
M.V. Zelinska
Keyword(s):  
Gene Polymorphism ◽  
Maintenance Therapy ◽  
Genomic Dna ◽  
Clinical Laboratory ◽  
Intensive Therapy ◽  
Venous Blood ◽  
Control Group ◽  
Drug Induced ◽  
Cyp2e1 Gene ◽  
Tb Treatment

Relevance. Investigation of polymorphism in a locus of CYP2E1 as the prognostic factor of drug-induced hepatotoxicity at anti-TB therapy is significant due to the influence of CYP2E1 on drug metabolism. The objective of the investigation is to analyze the association of rs2070676 СYP2E1 gene polymorphism with drug-induced hepatotoxicity by means of the clinical-laboratory values of serum transaminases at anti-TB treatment. Materials and methods. The study involved 47 patients with drug-susceptible tuberculosis first time discovered. 58 healthy volunteers comprised a control group. Laboratory indices were determined in venous blood three times: before the treatment as baseline; in 2 months of intensive therapy (isoniazid, rifampicin, ethambutol, pyrazinamide), then in 4 months of maintenance therapy (isoniazid, rifampicin). Serum activities of enzymes ALT, AST, and GGT were measured by standard algorithm on automatic analyzer BS-300. Analysis of rs2070676 polymorphism of CYP2E1 gene was performed by polymerase chain reaction using standard PureLink® Genomic DNA Kit for Purification of Genomic DNA; Manufacturer of INVITROGEN (USA). For statistical processing, IBM SPSS Statistics 23 was applied. Results. Investigation of serum ALT and AST in patients with major genotype CYP2E1 (C/C) showed the lower baseline ALT and AST levels comparing to the control group, which might be caused by suppression of hepatocytes functions at the development of the disease. Anti-TB treatment caused an increase in ALT and AST levels comparing to the baseline in patients with major CYP2E1 (C/C) genotype. In the group with C/G polymorphism, the baseline ALT level didn’t differ much from the baseline of the control group; it showed a decrease after intensive therapy and returned back to the initial level at maintenance therapy. This might be related to the certain protective property of СYP2E1 gene polymorphism. The AST level was increased after intensive therapy (to a smaller extent than for the patients with major C/C genotype) and remained on the same level at maintenance therapy. A study of GGT showed a gradual increase regardless of genotype. Conclusion. According to the data of the experiment, the status of hepatocytes in patients with tuberculosis at baseline and during treatment was different depending on the CYP2E1 genotype. The results of the experiment indicate that the CYP2E1 gene polymorphism has a certain protecting role. It reduces the level of drug metabolites and hepatotoxicity which causes mitochondrial dysfunction.

scholarly journals Study of cyp2E1 gene RsaI/PstI polymorphisms in patients with gastric cancer in north of Iran

2016 ◽  
Vol 4 (4) ◽  
pp. 22-27
Author(s):  
Samaneh Kamalipour ◽  
Ali Barzegar ◽  
Novin Nikbakhsh ◽  
Mohamad Shokrzadeh ◽  
◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cyp2e1 Gene ◽  
North Of Iran

Synonymous mutation rs2515641 affects CYP2E1 mRNA and protein expression and susceptibility to drug-induced liver injury

2020 ◽  
Vol 21 (7) ◽  
pp. 459-470
Author(s):  
Keguang Chen ◽  
Ruichen Guo ◽  
Chunmin Wei
Keyword(s):  
Gene Expression ◽  
Drug Disposition ◽  
Lentiviral Vector ◽  
Drug Induced ◽  
Drug Induced Liver Injury ◽  
Cyp2e1 Gene ◽  
Recombinant Cells ◽  
Mrna And Protein Expression ◽  
Cyp2e1 Expression ◽  
And Function

Aim: To evaluate whether the synonymous mutant rs2515641 could affect cytochrome P450 2E1 ( CYP2E1) expression and the response to acetaminophen (APAP) or triptolide (TP) treatment. Materials & methods: HepG2 cells were transfected with lentiviral vector containing either CYP2E1-1263C or CYP2E1-1263T. Some of these recombinant cells were then treated with APAP or TP. CYP2E1 gene expression was detected by PCR and western blot. Results: CYP2E1 gene expression decreased significantly both in mRNA and protein level after rs2515641 mutation, indicating that this polymorphism can affect both transcription and translation. Furthermore, rs2515641 mutation dramatically changes the response of CYP2E1 expression to APAP or TP treatment. Conclusion: Rs2515641 significantly changes CYP2E1 expression and function, which would be expected to affect drug disposition and response.

Effects of environmental factors on extrahepatic Cyp2e1 gene expression in mice

1998 ◽  
Vol 95 ◽  
pp. 101
Author(s):  
E. Sampol ◽  
P.H. Villard ◽  
F. Puyoou ◽  
E.M. Sérée ◽  
H. Point-Scoma ◽  
...  
Keyword(s):  
Gene Expression ◽  
Environmental Factors ◽  
Cyp2e1 Gene

0466 The effect of SNPs in the promoter on expression of CYP2E1 gene and boar taint

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 222-223
Author(s):  
H. E. Archer ◽  
M. Jafarikia ◽  
B. Lillie ◽  
F. Schenkel ◽  
E. J. Squires
Keyword(s):  
Boar Taint ◽  
Cyp2e1 Gene

scholarly journals Genetic Variants of Alcohol Metabolizing Enzymes and Alcohol-Related Liver Cirrhosis Risk

2021 ◽  
Vol 11 (5) ◽  
pp. 409
Author(s):  
Pedro Ayuso ◽  
Elena García-Martín ◽  
José A. Cornejo-García ◽  
José A. G. Agúndez ◽  
José María Ladero
Keyword(s):  
Liver Cirrhosis ◽  
Statistical Significance ◽  
Alcohol Exposure ◽  
Active Role ◽  
Copy Number Variations ◽  
Ethanol Metabolism ◽  
Public Health Issue ◽  
Excessive Alcohol Consumption ◽  
Cyp2e1 Gene ◽  
Wide Range

Alcohol-related liver disease (ARLD) is a major public health issue caused by excessive alcohol consumption. ARLD encompasses a wide range of chronic liver lesions, alcohol-related liver cirrhosis being the most severe and harmful state. Variations in the genes encoding the enzymes, which play an active role in ethanol metabolism, might influence alcohol exposure and hence be considered as risk factors of developing cirrhosis. We conducted a case-control study in which 164 alcohol-related liver cirrhosis patients and 272 healthy controls were genotyped for the following functional single nucleotide variations (SNVs): ADH1B gene, rs1229984, rs1041969, rs6413413, and rs2066702; ADH1C gene, rs35385902, rs283413, rs34195308, rs1693482, and rs35719513; CYP2E1 gene, rs3813867. Furthermore, copy number variations (CNVs) for ADH1A, ADH1B, ADH1C, and CYP2E1 genes were analyzed. A significant protective association with the risk of developing alcohol-related liver cirrhosis was observed between the mutant alleles of SNVs ADH1B rs1229984 (Pcvalue = 0.037) and ADH1C rs283413 (Pc value = 0.037). We identified CNVs in all genes studied, ADH1A gene deletions being more common in alcohol-related liver cirrhosis patients than in control subjects, although the association lost statistical significance after multivariate analyses. Our findings support that susceptibility to alcohol-related liver cirrhosis is related to variations in alcohol metabolism genes.

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