0466 The effect of SNPs in the promoter on expression of CYP2E1 gene and boar taint

AbstractBoar taint is an unpleasant odor in male pig meat, mainly caused by androstenone, skatole, and indole, which are deposited in the fat tissue. Piglet castration is the most common practice to prevent boar taint. However, castration is likely to be banished in a few years due to animal welfare concerns. Alternatives to castration, such as genetic selection, have been assessed. Androstenone and skatole have moderate to high heritability, which makes it feasible to select against these compounds. This review presents the latest results obtained on genetic selection against boar taint, on correlation with other traits, on differences in breeds, and on candidate genes related to boar taint. QTLs for androstenone and skatole have been reported mainly on chromosomes 6, 7, and 14. These chromosomes were reported to contain genes responsible for synthesis and degradation of androstenone and skatole. A myriad of work has been done to find markers or genes that can be used to select animals with lower boar taint. The selection against boar taint could decrease performance of some reproduction traits. However, a favorable response on production traits has been observed by selecting against boar taint. Selection results have shown that it is possible to reduce boar taint in few generations. In addition, modifications in diet and environment conditions could be associated with genetic selection to reduce boar taint. Nevertheless, costs to measure and select against boar taint should be rewarded with incentives from the market; otherwise, it would be difficult to implement genetic selection.

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Role of the Genetic Polymorphisms in the 5'-Flanking Region for Transcriptional Regulation of the Human CYP2E1 Gene

Alcoholism Clinical and Experimental Research ◽

10.1111/j.1530-0277.2006.00284.x ◽

2007 ◽

Vol 31 (s1) ◽

pp. S36-S42 ◽

Cited By ~ 14

Author(s):

Takayuki Uchimoto ◽

Sakae Itoga ◽

Masahiko Nezu ◽

Masahiko Sunaga ◽

Takeshi Tomonaga ◽

...

Keyword(s):

Transcriptional Regulation ◽

Genetic Polymorphisms ◽

Cyp2e1 Gene ◽

Flanking Region

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Endocrine Fertility Parameters—Genomic Background and Their Genetic Relationship to Boar Taint in German Landrace and Large White

Animals ◽

10.3390/ani11010231 ◽

2021 ◽

Vol 11 (1) ◽

pp. 231

Author(s):

Ines Brinke ◽

Christine Große-Brinkhaus ◽

Katharina Roth ◽

Maren Julia Pröll-Cornelissen ◽

Sebastian Klein ◽

...

Keyword(s):

Genetic Relatedness ◽

Genetic Relationships ◽

Young Male ◽

Boar Taint ◽

Genetic Progress ◽

Large White ◽

Genome Wide ◽

A Genome ◽

Different Origins ◽

Fertility Traits

The surgical castration of young male piglets without anesthesia is no longer allowed in Germany from 2021. One alternative is breeding against boar taint, but shared synthesis pathways of androstenone (AND) and several endocrine fertility parameters (EFP) indicate a risk of decreasing fertility. The objective of this study was to investigate the genetic background between AND, skatole (SKA), and six EFP in purebred Landrace (LR) and Large White (LW) populations. The animals were clustered according to their genetic relatedness because of their different origins. Estimated heritabilities (h2) of AND and SKA ranged between 0.52 and 0.34 in LR and LW. For EFP, h2 differed between the breeds except for follicle-stimulating hormone (FSH) (h2: 0.28–0.37). Both of the breeds showed unfavorable relationships between AND and testosterone, 17-β estradiol, and FSH. The genetic relationships (rg) between SKA and EFP differed between the breeds. A genome-wide association analysis revealed 48 significant associations and confirmed a region for SKA on Sus Scrofa chromosome (SSC) 14. For EFP, the results differed between the clusters. In conclusion, rg partly confirmed physiologically expected antagonisms between AND and EFP. Particular attention should be spent on fertility traits that are based on EFP when breeding against boar taint to balance the genetic progress in both of the trait complexes.

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Meta-analysis of the effect of immunocastration on production performance, reproductive organs and boar taint compounds in pigs

animal ◽

10.1017/s1751731112000146 ◽

2012 ◽

Vol 6 (8) ◽

pp. 1330-1338 ◽

Cited By ~ 63

Author(s):

N. Batorek ◽

M. Čandek-Potokar ◽

M. Bonneau ◽

J. Van Milgen

Keyword(s):

Meta Analysis ◽

Reproductive Organs ◽

Production Performance ◽

Boar Taint

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Revealing genetic relationships between compounds affecting boar taint and reproduction in pigs1

Journal of Animal Science ◽

10.2527/jas.2010-3290 ◽

2011 ◽

Vol 89 (3) ◽

pp. 680-692 ◽

Cited By ~ 39

Author(s):

E. Grindflek ◽

T. H. E. Meuwissen ◽

T. Aasmundstad ◽

H. Hamland ◽

M. H. S. Hansen ◽

...

Keyword(s):

Genetic Relationships ◽

Boar Taint

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CYP2E1-DEPENDENT VARIATIONS IN HEPATOCYTES DAMAGE DURING TREATMENT OF TUBERCULOSIS

Medical Science of Ukraine (MSU) ◽

10.32345/2664-4738.3.2020.4 ◽

2019 ◽

Vol 16 (3) ◽

pp. 20-26

Author(s):

L.V. Natrus ◽

L.V. Gayova ◽

O.O. Gorkunenko ◽

P.A. Chernovol ◽

M.V. Zelinska

Keyword(s):

Gene Polymorphism ◽

Maintenance Therapy ◽

Genomic Dna ◽

Clinical Laboratory ◽

Intensive Therapy ◽

Venous Blood ◽

Control Group ◽

Drug Induced ◽

Cyp2e1 Gene ◽

Tb Treatment

Relevance. Investigation of polymorphism in a locus of CYP2E1 as the prognostic factor of drug-induced hepatotoxicity at anti-TB therapy is significant due to the influence of CYP2E1 on drug metabolism. The objective of the investigation is to analyze the association of rs2070676 СYP2E1 gene polymorphism with drug-induced hepatotoxicity by means of the clinical-laboratory values of serum transaminases at anti-TB treatment. Materials and methods. The study involved 47 patients with drug-susceptible tuberculosis first time discovered. 58 healthy volunteers comprised a control group. Laboratory indices were determined in venous blood three times: before the treatment as baseline; in 2 months of intensive therapy (isoniazid, rifampicin, ethambutol, pyrazinamide), then in 4 months of maintenance therapy (isoniazid, rifampicin). Serum activities of enzymes ALT, AST, and GGT were measured by standard algorithm on automatic analyzer BS-300. Analysis of rs2070676 polymorphism of CYP2E1 gene was performed by polymerase chain reaction using standard PureLink® Genomic DNA Kit for Purification of Genomic DNA; Manufacturer of INVITROGEN (USA). For statistical processing, IBM SPSS Statistics 23 was applied. Results. Investigation of serum ALT and AST in patients with major genotype CYP2E1 (C/C) showed the lower baseline ALT and AST levels comparing to the control group, which might be caused by suppression of hepatocytes functions at the development of the disease. Anti-TB treatment caused an increase in ALT and AST levels comparing to the baseline in patients with major CYP2E1 (C/C) genotype. In the group with C/G polymorphism, the baseline ALT level didn’t differ much from the baseline of the control group; it showed a decrease after intensive therapy and returned back to the initial level at maintenance therapy. This might be related to the certain protective property of СYP2E1 gene polymorphism. The AST level was increased after intensive therapy (to a smaller extent than for the patients with major C/C genotype) and remained on the same level at maintenance therapy. A study of GGT showed a gradual increase regardless of genotype. Conclusion. According to the data of the experiment, the status of hepatocytes in patients with tuberculosis at baseline and during treatment was different depending on the CYP2E1 genotype. The results of the experiment indicate that the CYP2E1 gene polymorphism has a certain protecting role. It reduces the level of drug metabolites and hepatotoxicity which causes mitochondrial dysfunction.

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