scholarly journals Study of cyp2E1 gene RsaI/PstI polymorphisms in patients with gastric cancer in north of Iran

2016 ◽  
Vol 4 (4) ◽  
pp. 22-27
Author(s):  
Samaneh Kamalipour ◽  
Ali Barzegar ◽  
Novin Nikbakhsh ◽  
Mohamad Shokrzadeh ◽  
◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cyp2e1 Gene ◽  
North Of Iran

scholarly journals Correlation between the Expression Levels of miR-21 and miR-192 and Clinicopatological Features in Plasma of Patients with Gastric Cancer in Iran

2021 ◽  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Area Under The Curve ◽  
Tumor Stage ◽  
Expression Levels ◽  
The North ◽  
Study Results ◽  
Noninvasive Biomarker ◽  
North Of Iran ◽  
Gastric Cancer Patients

Background: Gastric cancer (GC) is the most prevalent malignancy worldwide and a common cause of death in Iran. Studies have proved that a variety of dysregulated microRNAs is involved in the development and progression of gastric cancer. The present study aimed to evaluate the expression levels of plasma circulating oncogenic miR-21 and miR-192 and their association with clinical phenotypes of patients with gastric cancer in the north of Iran. Material and Methods: Clinico-pathological analysis was conducted using a standard protocol and pathological tests. The expression levels of miR-21 and miR-192 were measured using quantitative reverse transcription-polymerase chain reaction in the plasma of twenty pre/post-operative gastric cancer patients and twenty healthy subjects. The receiver operating characteristic (ROC) curve of these microRNAs was analyzed to investigate their diagnosis properties. Results: The study results indicated that plasma miR-21 expression was significantly associated with tumor stage and helicobacter pylori infection status (P=0.024, P=0.0004, respectively). However, no association was observed between clinic-pathological characteristics and miR-192 expression. The results showed that the plasma levels of miR-21 (P=0.0001) and miR-192 (P=0.0007) were significantly higher in GC patients compared to those in healthy individuals. Furthermore, the ROC analyses yielded the area under the curve (AUC) values of 0.9525±0.03 (P<0.0001) and 0.5925±0.09 (P=0.316) for miR-21and miR-192, respectively. Pearson regression analysis showed that there was no significant correlation between the expression of miR-21 and miR-192 (P=0.1507). Conclusion: Based on the obtained results, the expression of the plasma level of miR-21 was significantly higher in gastric cancer patients compared to that in the healthy group. Furthermore, the higher levels of AUC in miR-21 indicated the potential role of miR-21 as a noninvasive biomarker for the prognosis of gastric cancer in the population of the north of Iran.

scholarly journals Correlation of –160C > A and –347GA > G polymorphisms in E-cadherin gene and gastric cancer in north of Iran

2021 ◽  
Vol 26 (1) ◽  
pp. 3
Author(s):  
MohammadBagher Hashemi-Soteh ◽  
Ramin Shekarriz ◽  
Reza Alikhani ◽  
Mohaddeseh Ghasemi ◽  
RezaAlizadeh Navaei
Keyword(s):  
Gastric Cancer ◽  
North Of Iran ◽  
E Cadherin

scholarly journals Determining the Effective Factors on Gastric Cancer Using Frailty Model in South-East and North of Iran

2017 ◽  
Vol 5 (3) ◽  
pp. 35-48 ◽  
Author(s):  
Roja Nikaeen ◽  
Alireza Khalilian ◽  
Abbas Bahrampour ◽  
◽  
◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Frailty Model ◽  
Effective Factors ◽  
North Of Iran

scholarly journals Evaluation of NOS2 Polymorphism in Gastric Adenocarcinoma Patients in Mazandaran Province

2021 ◽  
Author(s):  
Mahshad Hooshyar ◽  
Saeed Abedian-Kenari ◽  
Abbas Mohammadpour ◽  
Habibeh Mirmajidi ◽  
Majid Jafari-Sabet ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gastric Cancer ◽  
Control Group ◽  
Polymorphism Analysis ◽  
Mazandaran Province ◽  
Risk Area ◽  
Chi Square ◽  
High Risk Area ◽  
North Of Iran ◽  
Oxide Synthase

Introduction: Gastric cancer is the fourth most common cancer and the second leading cause of cancer death worldwide. North of Iran is a high-risk area for gastric cancer. Nitric oxide (NO), mainly synthesized by inducible nitric oxide synthase (NOS2) in pathological conditions, plays an important role in cytotoxicity, inflammation and fibrosis. In this research we studied the effect of (rs1137933) T>C genotype on gastric cancer. Methods: This analysis was performed on 93 patients with gastric cancer who were referred to endoscopy Tuba Clinic in 2015 and 93 healthy individuals as controls. DNA extracted from peripheral blood samples was applied in PCR-RFLP (Polymerase chain reaction- restriction fragment length polymorphism) analysis to determine (rs1137933) T>C genotype. The association of the (rs1137933) T>C genotype and gastric cancer risk were analyzed by MedCalc software and (X2) Chi-square and (OR) Odds Ratio exams. Results: Frequency of TT, CT, and CC genotypes in cases was 12.61, 51.35 and 36% and 38.7, 34.4, and 26.8% in the control group. Significant association was found between (rs1137933) T>C genotype with gastric cancer chance, P<0.05, OR=2/04, 95% CI (1/37 to 3/03). Conclusion: The results of the study show that the presence of CC+CT genotypes may increase the risk of gastric cancer.  P < 0.0001, OR=4.37 (2.17 to 8.80).Therefore, investigating the (rs1137933) T>C single nucleotide polymorphism of NOS2 gene could be an appropriate molecular marker that could be used to determine individual sensitivity to gastric cancer and for designing cancer prevention programs.

scholarly journals Demographic and clinical characteristics of gastric cancer patients in north of Iran, Mazandaran province, 2008-2014

2015 ◽  
Vol 1 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Seyed Khalil Mousavi ◽  
Ghasem Janbabai ◽  
Bizhan Kouchaki ◽  
Hanieh Borhani ◽  
Masoumeh Rashidi ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Patients ◽  
Clinical Characteristics ◽  
Mazandaran Province ◽  
North Of Iran ◽  
Gastric Cancer Patients

scholarly journals Evaluation of the Expression of Circulating miR-16 and miR-26a in the plasma of Gastric Cancer Patients in Guilan province, North of Iran

2021 ◽  
Keyword(s):  
Gastric Cancer ◽  
Smoking Status ◽  
Regulation Of Gene Expression ◽  
Expression Level ◽  
Real Time Quantitative Pcr ◽  
Non Invasive ◽  
Study Results ◽  
Significant Difference ◽  
North Of Iran ◽  
Gastric Cancer Patients

Background and Aims: Gastric cancer (GC) is a global health problem and the second deadly type of cancer worldwide with 1000 deaths per year. Poor prognosis in the early stages is one of the burdens in the treatment of GC. MicroRNAs are 18-22 nucleotide non-coding RNAs which play critical roles in the regulation of gene expression. Nowadays, miRNAs are widely known as non-invasive biomarkers for various kinds of cancers. This study aimed to evaluate the expression level of circulating miR-16 and miR-26a in GC patients and investigate the potential prognostic role of these miRNAs. Material and Methods: Initially, 20 plasma samples were obtained from pre-and post-operative GC patients, and the expression of miR-16 and -26a were compared with that of 20 healthy controls. The miRNAs expression was investigated using Real-Time quantitative PCR. The association between the expression levels of these miRNAs and clinicopathological features was also investigated in this study. Results: MiR-16 was down-regulated in GC patients; however, miR-26a expression revealed no significant difference between patients and controls in this regard. Furthermore, the expression of two miRNAs showed no association with the grade, TNM stage, and smoking status of the patients. Eventually, decreased expression of miR-16 was not correlated with the expression level of miR-26a. Conclusion: The downregulation of circulating miR-16 introduces this microRNA as a candidate biomarker for the non-invasive early prognosis of GC.

scholarly journals Gastric Precancerous Lesions in First Degree Relatives of Patients with Known Gastric Cancer: a Cross-Sectional Prospective Study in Guilan Province, North of Iran

2012 ◽  
Vol 13 (5) ◽  
pp. 1779-1782 ◽  
Author(s):  
Fariborz Mansour-Ghanaei ◽  
Farahnaz Joukar ◽  
Seyed Mohammad Baghaei ◽  
Mahmood Yousefi-Mashhoor ◽  
Mohammad Reza Naghipour ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Prospective Study ◽  
Precancerous Lesions ◽  
Cross Sectional ◽  
First Degree Relatives ◽  
North Of Iran ◽  
Guilan Province

Ultrastructural Cytochemical Study of Human Gastric Cancer: Observation of AKP ase,ACP ase,G6P ase,TPP ase and CO ase

1990 ◽  
Vol 48 (3) ◽  
pp. 254-255
Author(s):  
Dong Yuming ◽  
Yang Guanglin ◽  
Du Wei Dong ◽  
Xu Ai Liam
Keyword(s):  
Gastric Cancer ◽  
Gastric Epithelium ◽  
Human Gastric Cancer ◽  
Electron Microscopic ◽  
Cytochemical Study ◽  
Electron Microscopic Cytochemistry ◽  
Cytochemical Reaction ◽  
Set Up ◽  
Cancer Tissues ◽  
Cytochemical Technique

The activities and distributions of AKPase ,ACPase,G6Pase,TPPase and COase in human normal gastric mucosa and gastric cancer tissues were studied histochemically at light microscopic level. These enzymes are the marker enzymes of cell membrane lysosome endoplasmic reticulum, Golgi apparatus and mitochondrion objectively. On the basis of the research we set up a special ultrastructural cytochemical technique and first researched into gastric cancer domesticly. Ultrastructural cytochemistry is also called electron microscopic cytochemistry. This new technique possesses both the sensitivity of cytochemical reaction andi the high resolution of electron microscope. It is characterized by direct observation,exact localization and the combination morphology with function.The distributions of AKPase,ACPase,G6Pase,TPPase and COase in 14 cases of gastric cancer and 1 case of gastric Denign lesion were studied ultrastructurally. The results showed: 1. normal gastric epithelium had no AKPase reaction. The reaction of ACPase,G6Pase,TPPase and Coase were found in the corresponding organella, which were consistent with their function.

Ultrastructural Localization Study of Carcinoembryonic Antigen (CEA) in Gastric Cancer: Immunoelectron Microscopic Observation

1990 ◽  
Vol 48 (3) ◽  
pp. 252-253
Author(s):  
Dong Yuming ◽  
Yang Guanglin ◽  
Wu Jifeng ◽  
Chen Xiaolin
Keyword(s):  
Gastric Cancer ◽  
Intestinal Metaplasia ◽  
Cancer Cells ◽  
Microscopic Observation ◽  
Biological Significance ◽  
Ultrastructural Localization ◽  
Mucinous Carcinoma ◽  
Surface Glycoprotein ◽  
Tubular Adenocarcinoma ◽  
Pap Method

On the basis of light microscopic observation, the ultrastructural localization of CEA in gastric cancer was studied by immunoelectron microscopic technique. The distribution of CEA in gastric cancer and its biological significance and the mechanism of abnormal distribution of CEA were further discussed.Among 104 surgically resected specimens of gastric cancer with PAP method at light microscopic level, the incidence of CEA(+) was 85.58%. All of mucinous carcinoma exhibited CEA(+). In tubular adenocarcinoma the incidence of CEA(+) showed a tendency to rising with the increase of degree of differentiation. In normal epithelia and intestinal metaplasia CEA was faintly present and was found only in the luminal surface. The CEA staining patterns in cancer cells were of three types--- cytoplasmic, membranous and weak reactive type. The ultrastructural localization of CEA in 14 cases of gastric cancer was studied by immunoelectron microscopic technique.There was a little or no CEA in the microvilli of normal epithelia. In intestinal metaplasia CEA was found on the microvilli of absorptive cells and among the mucus particles of goblet cells. In gastric cancer CEA was also distributed on the lateral and basal surface or even over the entire surface of cancer cells and lost their polarity completely. Many studies had proved that the alterations in surface glycoprotein were characteristic changes of tumor cells. The antigenic determinant of CEA was glycoprotein, so the alterations of tumor-associated surface glycoprotein opened up a new way for the diagnosis of tumors.

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