Endometrial Stroma | ScienceGate

ABSTRACT 65 endometrial biopsies from castrated women who had received either natural or artificial sex hormone therapy were studied microscopically. Attention was paid to various histologic criteria, especially to the number of endometrial granulocytes (»K« cells, KZ). The following was obtained: The »K« cells are completely absent when no hormone substitution therapy is given. They were also lacking when the castrated patients were treated only with oestrogens, even if the dose given was ten-times that found in women during the reproductive ages. In contrast, the »K« cells developed from the endometrial stromal cells only under influence of progesterone, usually appearing first 8–10 days after the administration of the gestagen. The »K« cells were demonstrable in the number corresponding to a normal secretory phase only then, when the oestrogen-progesterone dosage ratio had induced a fully-developed secretory change, as measured by the usual histologic criteria. With an overdosage of oestrogen the »K« cells were either absent or were very sparse. Contrarily, an overdosage of progesterone had no influence on their number. The development of endometrial glands does not always entirely parallel that of the stroma in castrated patients following hormone therapy. A more exact indicator for the proper dose for the production of a secretory phase by hormone therapy seems to be the number of »K« cells in the endometrial stroma.

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COUP-TFII Regulates Human Endometrial Stromal Genes Involved in Inflammation

Molecular Endocrinology ◽

10.1210/me.2013-1191 ◽

2013 ◽

Vol 27 (12) ◽

pp. 2041-2054 ◽

Cited By ~ 32

Author(s):

Xilong Li ◽

Michael J. Large ◽

Chad J. Creighton ◽

Rainer B. Lanz ◽

Jae-Wook Jeong ◽

...

Keyword(s):

Cell Fate ◽

Cell Biology ◽

Embryo Implantation ◽

Orphan Nuclear Receptor ◽

Loss Of Function ◽

Mouse Uterus ◽

Endometrial Stromal Cells ◽

Endometrial Stroma ◽

Stroma Cell

Chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII; NR2F2) is an orphan nuclear receptor involved in cell-fate specification, organogenesis, angiogenesis, and metabolism. Ablation of COUP-TFII in the mouse uterus causes infertility due to defects in embryo attachment and impaired uterine stromal cell decidualization. Although the function of COUP-TFII in uterine decidualization has been described in mice, its role in the human uterus remains unknown. We observed that, as in mice, COUP-TFII is robustly expressed in the endometrial stroma of healthy women, and its expression is reduced in the ectopic lesions of women with endometriosis. To interrogate the role of COUP-TFII in human endometrial function, we used a small interfering RNA-mediated loss of function approach in primary human endometrial stromal cells. Attenuation of COUP-TFII expression did not completely block decidualization; rather it had a selective effect on gene expression. To better elucidate the role of COUP-TFII in endometrial stroma cell biology, the COUP-TFII transcriptome was defined by pairing microarray comparison with chromatin immunoprecipitation followed by deep sequencing. Gene ontology analysis demonstrates that COUP-TFII regulates a subset of genes in endometrial stroma cell decidualization such as those involved in cell adhesion, angiogenesis, and inflammation. Importantly this analysis shows that COUP-TFII plays a role in controlling the expression of inflammatory cytokines. The determination that COUP-TFII plays a role in inflammation may add insight into the role of COUP-TFII in embryo implantation and in endometrial diseases such as endometriosis.

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Endometrial Stromal Hyperplasia: An Underrecognized Condition

Case Reports in Pathology ◽

10.1155/2013/204082 ◽

2013 ◽

Vol 2013 ◽

pp. 1-4

Author(s):

Efthimios Sivridis ◽

Gerasimos Koutsougeras ◽

Alexandra Giatromanolaki

Keyword(s):

Low Grade ◽

Morphological Similarity ◽

Endometrial Stroma

Hyperplasia of the endometrial stroma is a poorly recognized lesion, lacking widespread recognition with most, if not all, such cases sequestrated in the literature as endometrial stromal nodules or low-grade endometrial stromal sarcomas. In this paper, we describe three examples of “endometrial stromal hyperplasia” which have a remarkable morphological similarity with the normally proliferating endometrial stroma and the endometrial stromal neoplasms, but which also possess subtle, but sufficient, differences to justify their taxonomic separation.

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Collagen VI and Laminin as Markers of Differentiation of Endometrial Stroma

Molecular and Cellular Aspects of Periimplantation Processes ◽

10.1007/978-1-4612-2548-5_22 ◽

1995 ◽

pp. 331-351 ◽

Cited By ~ 6

Author(s):

John D. Aplin ◽

Panayiota Mylona ◽

Cay M. Kielty ◽

Stephen Ball ◽

Jason D. L. Williams ◽

...

Keyword(s):

Collagen Vi ◽

Endometrial Stroma

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Blastocysts depict sex-specific signalling of IFNT transcription, translation and activity

Reproduction ◽

10.1530/rep-18-0312 ◽

2018 ◽

Author(s):

Corina Isabel Schanzenbach ◽

Sandra Milena Bernal-Ulloa ◽

Vera Anna van der Weijden ◽

Michael W. Pfaffl ◽

Mathias Büttner ◽

...

Keyword(s):

Biological Activity ◽

Antiviral Activity ◽

Protein Concentration ◽

Limit Of Detection ◽

Activity Level ◽

Enzyme Linked Immunosorbent Assay ◽

Protein Activity ◽

Interferon Tau ◽

Endometrial Stroma ◽

Gene And Protein Expression

Preimplantation bovine blastocyst supernatants exhibit sex-dependent antiviral activity, due to the ruminant pregnancy recognition signal Interferon tau (IFNT). Differing potencies of IFNT variants have been supposed as cause, although evidence remains scarce. Here, we aimed at quantifying the sex-dependent IFNT production on transcriptional, translational, and biological activity level in bovine blastocysts, to elucidate the origin of differences in antiviral activity between male and female blastocysts. Day 8 bovine blastocysts were co-cultured with endometrial stroma cells for 48 hours. The embryonic IFNT mRNA expression was determined by quantitative reverse transcription followed by polymerase chain reaction (RT-qPCR). Additionally, the IFNT protein concentration was determined using a sensitive in-house developed IFNT-specific Enzyme-linked-immunosorbent Assay (ELISA). The biological activity was assessed by quantifying the response of Interferon stimulated gene (ISG) expression in endometrial stroma cells. While the IFNT specific ELISA displayed a limit of detection of 7.3 pg/mL, the stroma cell culture system showed to react to as little as 0.1 pg/mL IFNT in RT-qPCR analysis. The female blastocysts had a significant, 5.6-fold, 3.6-fold, and 5.2-fold higher IFNT production than male blastocysts as determined by transcript abundance, protein concentration and, protein activity, respectively. Additionally, all parameters correlated positively, and therefore, we conclude that female blastocysts most likely have an increased IFNT gene and protein expression rather than expressing more potent IFNT variants.

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