Isolation and Characterization of Lytic Bacteriophages infecting Staphylococcus epidermidis

2016 ◽  
Vol 2 (1) ◽  
pp. 8-11
Author(s):  
Ream Saleem ◽  
Ghanim Aboud Jaber Al-Mola
Keyword(s):  
Staphylococcus Epidermidis ◽  
Plaque Assay ◽  
Sewage Water ◽  
Effect Of Temperature ◽  
Biochemical Tests ◽  
Phage Titer ◽  
Isolation And Characterization ◽  
Bacterial Lawn ◽  
Optimal Ph

The Staphylococcus epidermidis isolates were obtained during a period extended between September 2014 and January 2015, depending on biochemical tests and VITEK 2 system. Several sewage water samples were assayed using a plaque assay of double agar overlay as a source of S. Epidermidis phages. The bacteriophages were described depending on plaques size and shapes. Phage 1 was the most predominant in the bacterial lawn and able to infect other S. species such as S. aureus. Therefore, it was decided to study the effect of temperature on its original titer. The results revealed a gradual decrease in the phage titer with increasing dilution number. Each temperature at several incubation periods, significantly vary depending on phage titer. The optimum temperature was 40 ° C, while the 80 ° C was represented the inhibitor temperature. L.S.D. at level (0.05) for interaction was 39.552. The pH 6.5 – 7.5 were represented the optimal pH for the best phage activity while the phage titer beginning to decline in above and below this range of optimal pH , L.S.D. at level 0.05 was 17.898. In conclusion, our study found that Phage1 was considered as predominant phage because of their ability to infect other Staphylococci species such as S. Aureus.

scholarly journals Prevalence of Vibrio alginolyticus in Sediment Samples of River and Coastal Areas of Bangladesh

2016 ◽  
Vol 29 (1) ◽  
pp. 1-6 ◽  
Author(s):  
R Siddiqui ◽  
MM Alam ◽  
MN Naser ◽  
Y Otomo ◽  
M Yasmin ◽  
...  
Keyword(s):  
Fish Population ◽  
Vibrio Alginolyticus ◽  
Live Cells ◽  
Economic Damage ◽  
Biochemical Tests ◽  
Marine Animals ◽  
Isolation And Characterization ◽  
Vibrio Pathogens ◽  
Positive Results

Vibrio alginolyticus has been thought to be a halophilic marine bacterium that causes diarrhea, otitis media and wound infection through the consumption of raw or inappropriately cooked sea food. It is one of the main Vibrio pathogens affecting marine animals, such as marine fish, shrimp and shellfish which lead to large economic damage. Although there are reports on the presence of this organism in the coastal area of other countries, not so much work has been done on the isolation and characterization of this species in Bangladesh. The present study was, therefore, undertaken to isolate and characterize V. alginolyticus organisms isolated from the rivers (fresh water) and estuaries (brackish water) of Bangladesh. A total of 9 isolates of Vibrio species were obtained from different water bodies (three from Meghna river, two from Shangu river and four from estuary) and provisionally identified as Vibrio alginolyticus following standard biochemical tests. All these 9 strains showed same pattern of antibiotic resistance to ampicillin, streotomycin, penicillin, but sensitive to nalidixic acid. In the virulence properties test, two isolates showed positive results for toxR gene and none of the isolates showed positive results for tdh gene. Challenge experiments in Singhi fish (Heteropneustes fossi) with the live cells and the culture filtrate prepared from the V. alginolyticus showed high mortality of the fish population. All these studies suggest the presence of pathogenic V. alginolyticus strains in the river water and estuarine bodies of Bangladesh and the extracellular toxin(s) of the V. alginolyticus might be one of the causes for fish mortality.Bangladesh J Microbiol, Volume 29, Number 1, June 2012, pp 1-6

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

2015 ◽  
Vol 77 (31) ◽  
Author(s):  
Suganthi Thevarajoo ◽  
Chitra Selvaratnam ◽  
Kian Mau Goh ◽  
Fazilah Abd. Manan ◽  
Zaharah Ibrahim ◽  
...  
Keyword(s):  
16S Rrna ◽  
Marine Environment ◽  
Antibiotic Sensitivity ◽  
Marine Microorganisms ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
16S Rrna Sequence ◽  
Gram Staining ◽  
Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

scholarly journals Isolation and Characterization of Vibrio parahaemolyticus in the hepatopancreas of cultured white pacific shrimp - Litopenaeus vannamei

2020 ◽  
Vol 11 (1) ◽  
pp. 797-805
Author(s):  
Kolli Guna Ranjan ◽  
Girija Sankar G ◽  
Satyanarayana Raju DVV
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Bacterial Flora ◽  
Distance Matrix ◽  
Common Species ◽  
Genetic Identification ◽  
Molecular Technique ◽  
Microbial Culture ◽  
Biochemical Tests ◽  
Isolation And Characterization

Vibrio parahaemolyticus is the most common species among crustaceans, often causing various diseases and significant losses in aquaculture. Acute hepatopancreatic necrosis disease (AHPND) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. This species of bacteria is associated with gastrointestinal illness in humans and has been implicated in foodborne disease. The present study carried out, isolation and characterization of pathogenic bacterial flora isolated from the infected hepatopancreas of vannamei, obtained from various aquafarms in Andhra Pradesh, India, on 11th June 2018. The collected samples were plated on TCBS- (Thiosulfate-Citrate-Bile salt-Sucrose) agar medium and Hi -Chrome vibrio, as described in Bergey's manual of systematic bacteriology. Isolated colonies were subjected to the following tests- microscopic examination, growth at different temperatures, growth at different NaCl concentrations, and biochemical tests. Further purity, maintenance, and propagation of purified cultures were done. The microbial culture was identified using 16s rRNA molecular technique. Phylogenetic Evolutionary analyses and distance matrix were conducted in MEGA7.In the present study, different samples were screened, a total of three green colonies (V44, V45, V46) were isolated, identified by biochemical tests and genetic identification as Vibrio parahaemolyticus. A systematic methodology has been developed to isolate and characterize Vibrio sp. from diseased shrimp and identify them by genetic analysis

scholarly journals Isolation and Characterization of Photorhabdus Spp. (Enterobacteriaceae) Isolated From Heterorhabditis Bacteriophora in Kashere, Nigeria

2021 ◽  
Vol 25 (8) ◽  
pp. 1345-1348
Author(s):  
H.U. Aliyu ◽  
F. Tahir ◽  
E.B. Agbo ◽  
S.L. Kela
Keyword(s):  
Biological Control ◽  
Bacterial Isolate ◽  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Heterorhabditis Bacteriophora ◽  
Biochemical Tests ◽  
Susceptible Host ◽  
Isolation And Characterization ◽  
Biological Control Potential

Photorhabdus bacteria are symbiotically associated with the infective juveniles of entomopathogenic nematodes of the genus Heterorhabditis. These bacteria after infecting a susceptible host, produces a host of lethal toxins that killthe insect hosts within 24 to 72 hours. Consequently they have emerged and are trending as excellent biological control agents against insect pests of agricultural crops. Therefore, this study aim to isolate, identify and test the pathogenicity of Photorhabdus bacteria isolated from Heterorhabditis bacteriophora, entomopathogenic nematode from Kashere, Gombe State, Nigeria. To this end phenotypic and biochemical tests were conducted. The tests conducted showed that the isolate exhibited characteristics similar to those of Photorhabdus bacteria that have been identified. Last instar larvae of G. mellonella exposed to different concentrations of the bacterial isolate showed some level of susceptibility of the larvae to the bacterial isolate confirming its biological control potential.

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

scholarly journals Molecular characterization of Nocardiopsis species from Didwana dry salt lake of Rajasthan, India

2021 ◽  
Vol 13 (1) ◽  
pp. 396-401
Author(s):  
Khushbu Parihar ◽  
Alkesh Tak ◽  
Praveen Gehlot ◽  
Rakesh Pathak ◽  
Sunil Kumar Singh
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bioactive Compounds ◽  
Salt Lake ◽  
Bioactive Compound ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Multiple Sequence ◽  
Isolation And Characterization

The genus Nocardiopsis is well known to produce secondary metabolites especially antibacterial bioactive compound. Isolation and characterization of bioactive compounds producing novel isolates from unusual habitats are crucial. The present study was aimed to explore Didwana dry salt lake of Rajasthan state in India for the isolation and characterization of actinomycetes. The isolated actinomycetes isolates were characterized based on culture characteristics, biochemical tests and 16S rRNA gene sequencing. The 16S rRNA gene sequence analysis revealed that all the five isolates inhabiting soil of the said dry salt lake of Didwana, Rajasthan belonged to four species of Nocardiopsis viz., N. synnemataformans, N. potens, N. prasina and N. dassonvillei subsp. albirubida. The molecular identification based on 16S rRNA gene sequences was found accurate and robust. The phylogram generated through multiple sequence alignment of all the test isolates of Nocardiopsis revealed that the isolates aroused from a single branch and validated monophyletic association. The present study is the first report of exploring Nocardiopsis isolates from the dry salt lake. These characterized Nocardiopsis isolates isolated from Didwana dry salt lake habitat are novel stains and can be of significance in the detection and utilization of novel bioactive compounds.

Isolation and Characterization of a Highly Specific Serine Endopeptidase from an Oral Strain of Staphylococcus epidermidis

2001 ◽  
Vol 382 (7) ◽  
Author(s):  
Jonathan L. Moon ◽  
Agnieszka Banbula ◽  
Aneta Oleksy ◽  
John A. Mayo ◽  
James Travis
Keyword(s):  
Staphylococcus Epidermidis ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of Acetobacter aceti from rotten papaya

2016 ◽  
Vol 13 (2) ◽  
pp. 299-306 ◽  
Author(s):  
J Kowser ◽  
MG Aziz ◽  
MB Uddin
Keyword(s):  
Cell Mass ◽  
Single Pair ◽  
Acetobacter Aceti ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
The Media ◽  
Durham Tube ◽  
Successive Subculture ◽  
Dry Cell

The present study was concerned with the isolation and characterization of Acetobacter aceti from rotten papaya for vinegar production. The samples were inoculated in sterilized GYC standard media and then incubated at 30°C for 48 hours. Successive subculture was performed to screen out the strains. In Gram’s staining, the morphology of the isolated bacteria exhibited pink, small rod shaped single, pair and chain in arrangement, in the hanging drops technique, all the isolates revealed motile. Biochemical tests were performed by fermentation of five basic sugars by producing both acid and gas bubbles in Durham tube. All of the isolates were Indole, Voges-Proskauer (VP) and Oxidase negative, Methyl Red (MR) and Catalase positive. The growth rate of isolated strain was optimized by weighing dry cell and turbidity at 600 nm at different concentrations of dextrose (1%, 5% and 10%). Ten (10) percent dextrose solution showed rapid growth and higher cell mass than 5% and 1% solution respectively. Acidity of the media gradually increased from 0.102% to 2.18% from day 0 to day 7 and pH of the media decreased from 6.8 to 5.5 during the period. This protocol was successful for enriching Acetobacter aceti, which was essential for vinegar production.J. Bangladesh Agril. Univ. 13(2): 299-306, December 2015

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