scholarly journals Effect of the red light on the photosynthesis and phenolic accumulation in leaves of Hedyotis corymbosa (L.) Lam

2019 ◽  
Vol 3 (2) ◽  
pp. 128-135
Author(s):  
Le Anh Tuan ◽  
Phan Ngo Hoang ◽  
Seon-Ki Kim ◽  
Do Thuong Kiet
Keyword(s):  
Fluorescence Quenching ◽  
Light Exposure ◽  
Stem Elongation ◽  
Sugar Content ◽  
Fluorescent Light ◽  
Hill Reaction ◽  
Ex Vitro ◽  
Ps Ii ◽  
Red Led

Hedyotis corymbosa (L.) Lam a native herbaceous species containing many phenolic compounds is used in traditional medicine and medicinal technology. Phenolic acid, as well as many other secondary metabolites are photosynthetic-derived products. In this research, red LEDs (660 nm) and white fluorescent light were used to investigate the effects of different light sources on the photosynthesis and leaf phenolic compound accumulation of in vitro and ex vitro plants. Red LED (50 umol/m2/sec) promoted the stem elongation without changing plant biomass of in vitro plants. Increasing red LED intensities (from 50 to 100 or 150 umol/m2/sec) decrease maximum photochemical quantum yield of PS II (Fv/Fm) and coefficient of photochemical fluorescence quenching (qP), but stabilized electron transfer (ETR) and coefficient of non-photochemical fluorescence quenching (qN) of in vitro leaves. Under 100 umol/m2/sec of red LED, ex vitro leaf area, carotenoid contents, isolated chloroplast. Hill reaction and total sugar content were significantly reduced in comparison to those parameters from control plants under white light. Ex vitro plants' total carbohydrate contents were not statistically different the total leaf phenolic content of ex vitro plants under red LED light exposure was much higher than that the of control.  

scholarly journals Virulence of Aerial Conidia of Beauveria bassiana Produced under LED Light to Ctenocephalides felis (Cat Flea)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Sarayut Pittarate ◽  
Malee Thungrabeab ◽  
Supamit Mekchay ◽  
Patcharin Krutmuang
Keyword(s):  
Beauveria Bassiana ◽  
Adult Mortality ◽  
Ctenocephalides Felis ◽  
Fluorescent Light ◽  
Malt Extract ◽  
Led Light ◽  
Red Led ◽  
Aerial Conidia ◽  
Different Color

Ctenocephalides felis is an ectoparasitic flea species commonly found on dogs and cats. The current study verified the in vitro virulence of conidia of the entomopathogenic fungus Beauveria bassiana produced under different color LED light (red, blue, purple, green, yellow, and white) to adults of C. felis. The fungal isolates were cultivated on malt extract agar (MEA). Bioassay treatments used aerial conidia in test tubes. Adult fleas were obtained from a house cat in Chiang Mai province, Thailand. The experiments were composed of one control and eleven treatment groups. All of the treatments with B. bassiana conidia caused adult mortality after an exposure of 12 h. Among the conditions used in this study, B. bassiana cultured under red LED and fluorescent light were the most effective in causing mortality (100 %) in adult fleas after 36 h. The experimental results indicate that these aerial conidia of B. bassiana have promising potential for use in control of C. felis adult stages.

scholarly journals An RNA-Seq Analysis of Grape Plantlets Grown in vitro Reveals Different Responses to Blue, Green, Red LED Light, and White Fluorescent Light

2017 ◽  
Vol 8 ◽  
Author(s):  
Chun-Xia Li ◽  
Zhi-Gang Xu ◽  
Rui-Qi Dong ◽  
Sheng-Xin Chang ◽  
Lian-Zhen Wang ◽  
...  
Keyword(s):  
Fluorescent Light ◽  
Rna Seq ◽  
Led Light ◽  
Red Led

scholarly journals Effect of thidiazuron and monochromatic light on shoot development in vitro culture of Polygonum cuspidatum Sieb et Zucc

2020 ◽  
Vol 4 (2) ◽  
pp. First
Author(s):  
Doan Phan Phuong Thao ◽  
Tuan Anh Le ◽  
Phan Ngo Hoang
Keyword(s):  
Blue Light ◽  
Phenolic Content ◽  
Monochromatic Light ◽  
Total Phenolic ◽  
Fluorescent Light ◽  
Polygonum Cuspidatum ◽  
Blue Led ◽  
Red Led ◽  
Number Of Shoots

Polygonum cuspidatum Sieb. et Zucc, a medicinal plant, contains many phenolic compounds such as resveratrol. It has antioxidant, antibacterial, anti-cancer, HIV, and neuron protective properties. In this study, stem segments having an auxiliary bud were cultured in vitro on MS medium supplemented with TDZ at 0.1 mg/L for obtaining the highest number of shoots (8–9 shoots/cluster after eight weeks). The bud shoots were originated from the differentiation in the periphery of the lateral meristem. After eight weeks, the shoots cultured under the monochromatic light showed that the plant height, fresh and dry weight was higher than those grown under the fluorescent light at the same intensity of 50 µmol photon/m2/sec. Blue LED reduced the number of shoots/cluster, and respiration intensity of the inoculum compared to the red LED or fluorescent light conditions. However, after eight weeks, the total sugar and phenolic content in leaves and stems, as well as the resveratrol content of shoots under blue light, were always higher than under red LED or fluorescent light. Moreover, when samples were exposed under the one-week blue light condition at a double intensity (100 µmol photon/m2/sec), the shoot clusters growing under blue LED also had a remarkably high total phenolic content and significantly high resveratrol levels compared to the two other treatments. The roles of endogenous growth regulators in shoot clusters under different lighting conditions were also analyzed and discussed.

scholarly journals Evaluation of the effects of agricultural LED lighting on in vitro propagation of Cordyceps militaris (Link.) Fries

2020 ◽  
Vol 17 (3) ◽  
pp. 473-481
Author(s):  
Do Hong Gam ◽  
Duong Huong Huynh ◽  
Phan Thi Lan Anh ◽  
Nguyen Hoang Duong ◽  
Do Thi Kim Hoa
Keyword(s):  
Growth And Development ◽  
In Vitro Propagation ◽  
Positive Impact ◽  
The Other ◽  
Fluorescent Light ◽  
Led Lighting ◽  
Fresh Biomass ◽  
Red Led ◽  
Led Lights

In this study, the effects of various agricultural LED lights (LED NN), including single red LED (R), single blue LED (B), and four combinations of blue, red, and warm white (W) LED (BR, BRW1, BRW2, BRW3) on the growth and development of C. militaris (Link.) Fries were evaluated in vitro. After 7 days, samples subjected to LED NN showed shorter sporocarp sprouting time and higher sprouting ratio than the control, which was subjected to T5 fluorescent light. After 2 months, LED lights with high red ratio, such as single red LED and LED BR, had suppressing effect on the growth and development of C. militaris (Link.) Fries. On the other hand, combinations of red, blue, and warm white such as LED BRW1, LED BRW2, and LED BRW3 had the positive impact on the growth and development of this fungus. Notably, samples subjected to LED BRW2 reached 5.79 cm in height, fresh biomass of 3.67 g/20 samples. Cordycepin and Adenosine levels were 64.2 and 6.37 mg/100 g fresh mass, respectively. All of studied  indicators were the higher compared to those of the control and other LED lighting schemes. Therefore, it can be conlcuded that LED lighting combination with BRW2 ratio of 1:5:1 and luminous intensity of 45±2 µmol.m-2.s-1 (511,59 Lux) was suitable for the growth and development of C. militaris (Link.) Friesand a potential replacement of fluorescent light for C. militaris (Link.) Friesin vitro propagation.

scholarly journals Energy-Saving LED Light Affects the Efficiency of the Photosynthetic Apparatus and Carbohydrate Content in Gerbera jamesonii Bolus ex Hook. f. Axillary Shoots Multiplied In Vitro

Biology ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 1035
Author(s):  
Monika Cioć ◽  
Krzysztof Tokarz ◽  
Michał Dziurka ◽  
Bożena Pawłowska
Keyword(s):  
Energy Saving ◽  
Sugar Content ◽  
Photosynthetic Apparatus ◽  
Photochemical Quenching ◽  
Gerbera Jamesonii ◽  
Axillary Shoots ◽  
Glucose Content ◽  
Ps Ii ◽  
Led Light

An energy-saving light emitting diode (LED) system allows for adjustment of light quality, which affects plant development and metabolic processes in in vitro cultures. The study investigated the content of endogenous carbohydrates and the condition of the photosynthetic apparatus of Gerbera jamesonii Bolus ex Hook. f. Our aim was to analyze the effects of different LED light qualities—100% red light (R LED), 100% blue (B LED), a mixture of red and blue (7:3) (RB LED), and a fluorescent lamp as a control (Fl)—during the multiplication of axillary shoots. After 40 days, the culture measurements were performed using a non-invasive pulse amplitude modulation (PAM) fluorimeter. Sugar content was assessed with high performance liquid chromatography (HPLC). Two forms of free monosaccharides (glucose and fructose), two sugar alcohol derivatives (inositol and glycerol), and seven forms of free oligosaccharides were identified. Of those, glucose content was the highest. LEDs did not disturb the sugar metabolism in multiplied shoots. Their monosaccharides were three times more abundant than oligosaccharides; the same results were found in plants grown under control light. R light depleted the performance of the photosynthetic apparatus and caused its permanent damage. The RB LED spectrum ensured the most efficient non-photochemical quenching of the photosystem II (PS II) excitation state and high shoot quality.

scholarly journals In vitro seed germination and seedling growth of Calanthe discolor Lindl

2015 ◽  
Vol 71 (1) ◽  
pp. 109-119 ◽  
Author(s):  
Kee Hwa Bae ◽  
Kyoung Hee Oh ◽  
Soo-Young Kim
Keyword(s):  
Seedling Growth ◽  
Culture Media ◽  
Suitable Condition ◽  
Fluorescent Light ◽  
Immature Seeds ◽  
Red Led ◽  
Led Lights ◽  
Protocorm Formation ◽  
Germination And Growth

Abstract We investigated the effects of sodium hypochlorite (NaOCl) and culture medium on embryo swelling and germination of Calanthe discolor Lindl., and established a method for determining the swelling and protocorm formation of C. discolor seeds via in vitro examination of immature seeds. Treatment of immature seeds with NaOCl greatly enhanced the extent of embryo swelling and protocorm formation of immature zygote embryos compared to seeds without NaOCl treatment. The effects of the culture media were also evaluated with regard to embryo swelling and protocorm formation of in vitro cultured seeds with and without NaOCl treatment. Additionally, the effects of white fluorescent light and red and blue LED lights on seedling growth in in vitro culture were examined. The most suitable condition for seedling growth after 12 weeks of culture was the red LED light with POM medium. These results show effective asymbiotic germination and growth of C. discolor.

FLUORESCENT LIGHT (FL), RED LED AND BLUE LED SPECTRUMS EFFECTS ON IN VITRO SHOOTS MULTIPLICATION

2016 ◽  
Vol 78 (6-6) ◽  
Author(s):  
Nurul Syahirah Azmi ◽  
Robiah Ahmad ◽  
Rusli Ibrahim
Keyword(s):  
Large Scale ◽  
Ornamental Plants ◽  
Fruit Production ◽  
Fluorescent Light ◽  
Alternative Source ◽  
Light Emitting ◽  
Blue Led ◽  
In Vitro Shoots ◽  
Red Led

Tissue culture in ornamental plants is one of the relevant factors that beat production of vegetables and fruit production worldwide. It has been recognized as an effective tool to enhance large scale of plant multiplication. However, the conventional lighting system may contain unnecessary wavelength that are low quality to promote growth. In this study, experiment was conducted by using Light Emitting Diodes (LED) as an alternative source of lighting. Red and blue LEDs along with fluorescent light (FL) were applied to determine the best source of light in multiplication of rose. Under the same media regimes which are MS media basal and BAP shoot hormone, blue LED had shown more shoots and leaves. 

scholarly journals INFLUENCE OF LIGHT ON IN VITRO ROOT CHARACTER FOR WOODY PLANT MICROCUTTINGS.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1117a-1117
Author(s):  
Renee Timmermann ◽  
M.A.L. Smith
Keyword(s):  
Root System ◽  
Light Exposure ◽  
Root Hairs ◽  
Woody Species ◽  
Major Influence ◽  
Root Initiation ◽  
Ex Vitro ◽  
Wide Range ◽  
Root Character

The structure and quality of the adventitious root system formed on a microcutting stem is crucial to the successful acclimation, survival, and ultimate performance of micropropagated plants. Despite increasing evidence that the rooting method impacts on the character of the framework root system, very little research has elucidated the consequences of standard rooting methods on plant quality and performance. Root initiation on microcuttings is handled by a wide range of strategies in commercial practice. In comparison to in vitro roots, ex vitro roots have smaller root diameters, larger vascular diameters, greater length, more branch root development, and root hairs. One important microenvironmental parameter - light - was investigated to determine its contribution to root character. Typically, in vitro root systems are exposed to light throughout the root initiation period. Parallel treatments were established of in vitro light and dark rooting and ex vitro rooting of four woody species. Regardless of light exposure, the overall diameter of adventitious roots was larger for in vitro treatments than the ex vitro treatment. Vascular development was significantly more advanced ex vitro. These results suggest that light is not a major influence contributing to the differences between ex vitro and in vitro root character.

scholarly journals Morphophysiological development of Tabeluia serratifolia Vahl Nich. seeds

2008 ◽  
Vol 65 (6) ◽  
pp. 643-651 ◽  
Author(s):  
Maria Laene Moreira de Carvalho ◽  
Marcela Carlota Nery ◽  
Luciana Magda de Oliveira ◽  
Henk W. M. Hilhorst ◽  
Renato Mendes Guimarães
Keyword(s):  
Moisture Content ◽  
Dry Matter ◽  
Sugar Content ◽  
Dry Matter Accumulation ◽  
Seed Color ◽  
Maturation Process ◽  
Ex Vitro ◽  
Physiological Maturity ◽  
Heat Resistant

Tabebuia serratifolia is used for the reforestation of degraded areas. Despite protection by law for permanent preservation, the species is in danger of extinction due to improper exploitation. With the objective to aid preservation and long term storage of the species we evaluated morphophysiological alterations of T. serratifolia seeds during the maturation process in order to identify markers that can be used for harvesting and storage. Fruits were collected at anthesis and seven developmental stages from trees growing in Lavras, state of Minas Gerais, Brazil. At each harvest, fruits and seeds were evaluated for color and size, moisture content, dry matter, internal morphology (by X-ray analysis), germination parameters (in vitro and ex vitro), as well as sugar and polyphenol content and heat resistant proteins. During the maturation process the initially green fruits changed to a brownish color and grew from a length of 7 to 18 cm; cracks appeared at the beginning of seed dispersal. The seed color varied from leaf-green to brownish and the length from 1 to 3 cm. The first indicatior of physiological maturity should be observed at 39 days post-anthesis, when variations the color and size of both fruits and seeds were observed. Increase in the moisture content, dry matter and germination, percentage of seeds and embryos in vitro, as well as a reduction in sugar content and LEA proteins were also observed. The physiological maturity of T. serratifolia seeds was reached 53 days after anthesis, coinciding with a maximum of dry matter accumulation and germination (and index of germination speed ex vitro), decrease in phenol levels, higher intensity of heat-resistant protein bands and the beginning of fruit opening.

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