scholarly journals Influence of ecological and geographical features of breeding brown Carpathian rocks on its genetic structure

2019 ◽  
Vol 21 (91) ◽  
pp. 103-107
Author(s):  
V. Y. Bodnaruk ◽  
L. I. Muzyka ◽  
A. J. Zhmur ◽  
T. V. Orikhivskyj
Keyword(s):  
Genetic Structure ◽  
Genetic Analysis ◽  
First Generation ◽  
Molecular Genetic ◽  
Breeding Value ◽  
Population Genetic Analysis ◽  
Geographical Regions ◽  
Biochemical Systems ◽  
Genetically Determined ◽  
F1 Generation

Studying the genetic structure of cattle makes it possible to characterize it by molecular genetic markers, which in the future enable effective breeding work with animals breed to achieve the desired characteristics. Lately is becoming more apparent the need to preserve genetic diversity namely due to the peculiarities of breeding in different ecological and geographical regions. Therefore, we conducted an investigation of the genetic structure of animals of pure-bred Brown Carpathian (BC), Schwyz (S) breeds, their mixtures of the first (F1) generation (1/2BС+1/2S), Brown Carpathian with a share of hereditary breed of Schwyz breed up to 50 and more than 50%, as well as Brown Carpathian, raised in flat and mountain farms. Genetic structure was evaluated by genetically determined polymorphism of groups of genetic-biochemical systems. Of the 14 investigated loci that encode proteins and blood enzymes, polymorphism was identified in five loci, namely: transferrin (Tf), ceruloplasmin (Cr), amylase-1 (Am-1), hemoglobin (Hb), and purine nucleoside phosphorylase (NP). As a result of population-genetic analysis of polymorphic loci of proteins and enzymes of blood in animals of Brown Carpathian breed the following data were obtained:the highest level of heterozygosity (H) was found at the transferrin locus – 95%, the lowest – at the hemoglobin locus – 9.5%.The heterozygosity at the ceruloplasmin locus was 68%. Evaluation of heterozygosity at the purinnucleoside phosphorylase locus was not possible because part of the animals with the NP-H (high activity) phenotype could carry a low activity variant in the heterozygous state. For all polymorphic systems in the Brown Carpathian breed genotype frequency distribution was in a balanced state according to Hardy-Weinberg law, except for the transferrin locus,where a statistically significant deficiency of homozygotes (P > 0.95) in the most common allelic variants is detected. Analysis of the dendrogram showed that groups of animals of Brown Carpathian breed with different proportion of hereditary Schwyz form one cluster that is clearly differentiated from purebred schwyzes. First-generation domestic animals (F1), by genetic structure, are significantly closer to the maternal Brown Carpathian breed than to the Schwyz one. Thus, the data obtained indicate that the specific characteristic of Brown Carpathian breed may be a relatively increased breeding value of a number of allelic interlocal associations, in which the loci of the genetic-biochemical systems are involved in one way or another. Genetic analysis of these systems showed the presence in the breed of its specific features, which are specific to it.

scholarly journals Genofond of some breed of cattle

2017 ◽  
Vol 19 (74) ◽  
Author(s):  
V. Bodnaruk ◽  
Z. Shchebatyj ◽  
L. Muzyka ◽  
A. Zhmur ◽  
T. Orikhivskyj
Keyword(s):  
Genetic Structure ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Structural Features ◽  
Histochemical Staining ◽  
Test Tube ◽  
Genetic Characteristics ◽  
Biochemical Systems ◽  
High Level ◽  
Genetically Determined

Genetic investigations of cattle breeds in Western Ukraine are environmentally necessary, as well as they are the most relevant in connection with the formation of rock associations – for example Carpathian Brown. Addition to the study of genetic characteristics of different breeds to be used in agricultural production - it is an attempt to identify in the herd and genetically determined feature, which occurs most frequently. The choice of such economically valuable features determined by modern methods of agriculture research, and basically are chosen herds with such gene fond in which this feature is most typical. The genetic structure was evaluated for the genetically determined polymorphism groups of genetic and biochemical systems. Experiments were conducted on red blood cells and plasma. The blood of animals was taken from the jugular vein in a test tube with heparin. Polymorphism of proteins and enzymes was evaluated using the method of electrophoretic separation of proteins in 13% of starch gels in horizontal chambers with further histochemical staining. Each species is characterized by its own structural features of the genetic structure. According to locus of  transferrin breeds can be divided into two groups: with the dominance of allelic variant Tf A (Pintshau, Black and Spotted Holstein, Red and Spotted Holstein) and domination of variant Tf D2 (Symentals, Schwyz, Gray Ukrainian). Animals of Brown Carpathian breed have a large number of heterozygotes Tf AD2 (43%). The group of Schwyz has relatively high heterozygosity (49%). Groups of Black-Spotted and Red- Spotted Holstein are similar. When analyzing the genetic structure for the locus AM-1 can be noted the similarity of samples of animal Pintshau, Schwyz, Carpathian of brown rocks. In animals of Gray Ukrainian breed the frequency of allelic variants display of AM-1 was the highest and amounted to – 0.933. For ceruloplasmin locus significant differences were not found, except Gray Ukrainian breed animals where frequency of display of CP A was quite high and amounted to 0.733 and Simentales where is marked the high level of heterozygosity – 84%. At electrophoretic distillation of washed red blood cells of studied groups of animals were found two phenotypes of purine nucleoside phosphorylase: one is associated with high activity (H), the other with low activity (L).

scholarly journals Study of human thyroglobulin gene polymorphism

1996 ◽  
Vol 42 (5) ◽  
pp. 34-36
Author(s):  
D. A. Kadyrova ◽  
B. A. Atakhanova ◽  
M. L. Rakhimova ◽  
G. D. Umarova ◽  
Ya. Kh. Turakulov
Keyword(s):  
Genetic Analysis ◽  
Congenital Hypothyroidism ◽  
Population Genetic ◽  
Molecular Genetic ◽  
Restriction Enzymes ◽  
Healthy Individuals ◽  
Population Genetic Analysis ◽  
Genetic Studies ◽  
Blood Samples ◽  
Thyroglobulin Gene

The main goal of this work was to conduct a population genetic analysis of RFLP detected in the TG gene and to develop a system of molecular genetic studies of hereditary thyroid diseases associated with polynucleotide rearrangements in the structure of TG. A population genetic analysis of RFLP detected in the TG gene was carried out. When splitting blood samples of healthy Tashkent residents with EcoRV and Taql restriction enzymes, 2 pairs of alternative variants of normal RFLP were detected - 13.8 or 8.5 and 5.8 or 6.2 bp respectively. In order to detect RFLP in the TG gene for congenital hypothyroidism, DNA samples from 2 families (mother, father, daughter) with a clinical diagnosis of congenital hypothyroidism were analyzed and the same variants of RFLP found in healthy individuals.

scholarly journals Genetic structure of the Chernivtsi local stock of rainbow trout (Oncorhynchus mykiss) as determined by SSR-markers

2019 ◽  
Vol 25 ◽  
pp. 26-31
Author(s):  
O. Yu. Bielikova ◽  
O. Zaloilo ◽  
S. Тarasjuk ◽  
A. Mruk ◽  
V. Romanenko
Keyword(s):  
Rainbow Trout ◽  
Genetic Structure ◽  
Ssr Markers ◽  
Molecular Genetic ◽  
Individual Identification ◽  
High Tech ◽  
Effective Number ◽  
Population Genetic Analysis ◽  
Mean Values ◽  
Local Herd

Aim. The trend towards the development of high-tech trout farms is observed in Ukraine. The analysis of the genetic structure of rainbow trout by molecular genetic methods is necessary for breeding work. Therefore, the purpose of the study was to study polymorphism at the microsatellite loci of Oncorhynchus mykis of the chernivetsk local herd. Me-thods. The determination of the genetic polymorphism was carried out according to indicators: the effective number of alleles on the locus (ne), the observed (Но) and expected (Hе) heterozygosity, indexes of fixation (Fis) and polymorphism (PIC). Results. Specific features of the structure of the gene pool of the local herd O. mykiss by the SSR loci OMM 1032, 1077, 1088, STR 15, 60, 73 were revealed. The average value of the effective number of alleles per locus was 3.87. The high average values of the heterozygosity of the local herd and the index of fixation were fixed (mean values of H e and F is: 0.73 and -0.14, respectively). А high index of polymorphism was recorded for all used DNA markers and was 0.69. Тhe possibility to analyse the genetic structure of salmon using these SSR-markers was substantiated. Conclusions. An analysis of the genetic structure of trout (O. mykiss) was performed by using of 6 microsatellite. It was shown that the investigated local herd has a high level of heterozygosity. The conducted studies have shown the effectiveness of using selected loci for individual identification and population-genetic analysis. Keywords: Oncorhynchus mykis, SSR-markers, heterozygotes, polymorphism.

scholarly journals Population genetic analysis of the population of Transcarpathian region based on surname data

2019 ◽  
Vol 25 ◽  
pp. 44-48
Author(s):  
M. Yu. Gorpynchenko ◽  
L. A. Atramentova
Keyword(s):  
Genetic Structure ◽  
Genetic Analysis ◽  
Population Genetic ◽  
Population Genetic Analysis ◽  
Migration Index ◽  
Index Of Entropy ◽  
Total List ◽  
Regional Population ◽  
And Migration ◽  
The Way

Aim. The purpose of research is to analyse the populations of Transcarpathian region, finding out, as ability of surnames changes to differentiate the populations of area level as compared with regional. Methods. The base of total list of surnames of the population of Transcarpathian region is used. The way of description of genetic structure of the population offered I. Barrai. Material is analysed with use of methodical approaches developed of Е.В. Balanovskaya. Results. Population genetic ratings for area as a whole - index of isonymy (І=6,1×10-4), index of inbreeding (FST=15,2×10-5), migration index (v=12,8×10-4), rate of names diversity (a=16,4×102), index of entropy (H=12,8) and redundancy of surnames (R=36,7), as well as for thirteen areas of Transcarpathian region are designed. Conclusions. Index isonymy, inbreeding and migration in a few times above in the populations of area level, than regional, the rating of diversity in area populations has more low values, than in regional population. Keywords: quasigenetic markers, population, isonymy, inbreeding, migration.

scholarly journals Polymorphism of Genes of the Protein and Lipid Exchanges in Modern Ukranian Breeds of Cattle Bred for Dairy Productivity

2020 ◽  
Vol 74 (6) ◽  
pp. 373-380
Author(s):  
Yulia V. Gritsienko ◽  
Michael I. Gill ◽  
Liubov Denisyuk ◽  
Igor Yu. Gorbatenko
Keyword(s):  
Transcription Factor ◽  
Genetic Structure ◽  
Restriction Fragment Length Polymorphism ◽  
Genetic Analysis ◽  
Fragment Length Polymorphism ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Chain Reaction ◽  
Lipid Exchange ◽  
Restriction Fragment Length

AbstractGenetic structure of animals of several Ukrainian modern breeds analysed by genes of protein and lipid exchange CSN3 (kappa-casein), BLG (betalactaglobulin), LEP (leptin), Pit-1 (pituitery transcription factor), TG-5 (thyroglobulin) were investigated in cows of three domestic breeds (Ukrainian Red Dairy, Ukrainian Black-speckled Dairy, Ukrainian Red-speckled Dairy). Polymer-ase chain reaction, followed by restriction fragment length polymorphism was used for the molecular-genetic analysis.

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