TROMBOSIT TERENDAH, KENAIKAN HEMATOKRIT DAN KADAR TNF-α SEBAGAI INDIKASI KEPARAHAN INFEKSI VIRUS DENGUE PADA PASIEN DD DAN DBD

2019 ◽  
Vol 10 (01) ◽  
pp. 13-22
Author(s):  
A.S. Siskayani ◽  
I Made Sumarya ◽  
N.L.P Kartika Sari
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Hemorrhagic Fever ◽  
Hemorrhagic Diathesis ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Blood Samples ◽  
Tnf Α ◽  
The Mean

Clinical manifestations of dengue virus infection vary from unspecified fever, dengue fever (DF) and dengue hemorrhagic fever (DHF). The fundamental difference in the diagnosis between DF and DHF is plasma leakage, hypotension, thrombocytopenia and hemorrhagic diathesis in DHF. The objectives of the study were to determine the lowest platelet, haematocrit increase, and TNF-α levels in DF and DHF patients as an indication of severity of dengue virus infection. The cross-sectional study was conducted by taking 54 blood samples of patients aged 26-45 years who were infected by dengue virus and grouped into two groups: DF patient group (27 blood samples) and DHF group (27 blood samples). The lowest platelets and hematocrit rise were determined by Hematology Analyzer and the TNF-α levels were determined by ELISA. The data obtained were statistically analyzed by independent Mann-Whitney test. The results showed that the lowest mean platelet counts of DF patients were significantly (p <0.01) higher than those of DHF patients. The mean hematocrit increase in DF patients was significantly (p <0.01) lower than that of the DHF patients and the mean TNF-α levels of DF patients were significantly (p <0.01) lower than the DHF patients. Based on the results of this study it can be concluded that the lowest platelets, hematocrit increase and TNF-α levels are an indication of the severity of dengue virus infection.

scholarly journals The Role Activity of Complement, TNFα & IL12 in Pathogenesis Dengue Virus

2010 ◽  
Vol 1 (1) ◽  
pp. 44
Author(s):  
Soegeng Soegijanto ◽  
Dian Dwi Sary ◽  
Budi Setiawan ◽  
Atsushi Yamanaka
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Dengue Fever ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
Study Groups ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Complement Activity ◽  
Grade Iii

Dengue Virus infection is always found in some part of the world especially South East Asia including Indonesia. The pathogenesis of Dengue Virus infection is still controversial. The aim of this study is to analyze the role complement activity, TNFα & IL12 in Dengue Virus infection especially in pathogenesis of Dengue Virus infection. Cross sectional study had been done since February 2009 in Dr. Soetomo Hospital Surabaya. Blood Sera of Dengue Virus infection were collected from Dengue Fever, and Dengue Hemorrhagic Fever patient who had been care in Paediatric. Dengue patients and time schedule for taking blood sample for examination CH50, TNFα & IL12 as follow: on the first day on admission, the second day, the third day. Study groups of patients as follow: Dengue Fever, 36; Dengue Hemorrhagic Fever grade I, 37; Dengue Hemorrhagic Fever grade II, 10; Dengue Hemorrhagic Fever grade III, 18; Dengue Hemorrhagic Fever grade IV, 6. In this study found that the higher activity complement which lower level CH50 was more identified on Dengue Shock Syndrome and Dengue Hemorrhagic Fever grade III than Dengue Fever cases. A concept of our study was focusing on manifestation of vascular leakage, measurement of complement activity CH50, TNFα & IL12 and clinical manifestation Dengue Hemorrhagic Fever. The examination of TNFα & IL12 in our study supported the role the activity complement. The conclusion are measurement CH50, TNFα & IL12 can be used as a predictive factor of the degree of Dengue Virus infection

scholarly journals LIMFOSIT PLASMA BIRU DAN JUMLAH LEUKOSIT PADA PASIEN ANAK INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Rosnatalia D. Arruan ◽  
Glady Rambert ◽  
Firginia Manoppo
Keyword(s):  
Informed Consent ◽  
Virus Infection ◽  
Dengue Virus ◽  
Laboratory Test ◽  
Dengue Hemorrhagic Fever ◽  
Blood Test ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Routine Blood

Abstract: Dengue is an arboviral disease that is widely spread across the world by Aedes mosquitoes. The early onset of dengue virus infection is difficult to identify due to indistinctive clinical symptoms. Therefore, simple laboratory test is required in the early diagnosis of dengue virus infection. Laboratory test that include routine blood test and blue plasma lymphocyte are important as rapid and relatively cheap diagnostic tool. Blue plasma lymphocytes derived from lymphoid which acts as an immune response that can be found in 4% of peripheral blood on 98% dengue hemorrhagic fever cases. Besides the increases in blue plasma lymphocytes, dengue virus infection can also causes leucopenia. Leucopenia in Dengue Hemorrhagic Fever (DHF) is characterized by the decrease of neutrophile count that happened during the early critical phase (third day of fever). This is a cross-sectional study using samples of 37 children who are infected with dengue virus in RS Advent Manado, RS TNI AD Wolter Monginsidi, RS Pancaran Kasih Manado during December 2014 to January 2015. Inclusion criteria are children below age 15, who have NS1 or IgG/IgM positive, who are on the third until seventh day of fever, whose parents or guardian have signed the informed consent. Conclusion: Leucopenia (leukocyte count below 4000/mm3) was found in children with dengue virus infection who had their routine blood test. Blue plasma lymphocytes are not always found in patients with NS1-AG, IgG/IgM positive.Keywords: dengue virus infection, leucocyte, blue plasma lymphocyteAbstrak: Dengue adalah penyakit arboviral yang paling banyak tersebar di dunia yang ditularkan nyamuk Aedes. Awal infeksi virus dengue sulit untuk dikenali sebab tidak menunjukkan gejala klinis khas. Karena itu, pemeriksaan laboratorium sederhana dapat berperan dalam pengenalan infeksi virus, adalah pemeriksaan darah rutin dan limfosit plasma biru yang cepat dan murah. Limfosit plasma biru sebagai respon imun dengan persentase 4% di darah tepi pada 98% kasus demam beradarah dengue. Selain peningkatan limfosit plasma biru, infeksi virus dengue juga dapat menyebabkan leukopenia. Leukopenia pada demam berdarah dengue didominasi penurunan jumlah neutrofil yang terjadi pada awal fase kritis (hari ketiga demam). Penelitian ini menggunakan desain cross sectional dengan sampel penelitian 37 pasien anak yang terinfeksi virus dengue di RS Advent Manado, RS TNI AD Wolter Mongisidi, RS Pancaran Kasih Manado pada bulan Desember 2014 sampai Januari 2015. Kriteria masukan adalah Anak usia kurang dari 15 tahun, hari ketiga sampai ketujuh demam, NS1 atau IgG/IgM positif, Orangtua atau wali bersedia menandatangani informed consent. Simpulan: Pada pasien anak dengan infeksi virus dengue yang dilakukan pemeriksaan darah rutin ditemukan adanya leukopenia dengan jumlah leukosit dibawah 4000/mm3.Kata kunci: infeksi virus dengue, leukosit, limfosit plasma biru

scholarly journals UPDATE MANAGEMENT OF DENGUE COMPLICATION IN PEDIATRIC

2015 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Soegeng Soegijanto
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Performance ◽  
Kidney Injury ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Liver Involvement ◽  
Dengue Virus Infection ◽  
Important Health ◽  
Grade Iii

Dengue virus infection is one of the important health problems in Indonesia, although the mortality rate has been decreased but many dengue shock syndrome cases is very difficult to be solving handled. It might be due to nature course of dengue virus infection is very difficult to predict of the earlier time of severity occur. THE AIM To get idea to make update management of dengue complication in pediatric. MATERIAL AND METHOD Data were compiled from Dr. Soetomo Hospital Surabaya in 2009. The diagnosis of all cases was based on criteria WHO 1997 and PCR examination in Institute Tropical Disease for identified serotype of dengue virus infection. The unusual cases of dengue virus infection were treated following the new WHO protocol in 2009. RESULT There were only 3 cases with serotype DEN 1, consisted 2 cases had age 1–4 years and 1 had age 5–14 years. 2 cases showed a severe clinical performance as dengue shock syndrome and 1 case showed as unusual case of dengue virus infection. Three report cases of: a. Dengue hemorrhagic fever grade III which liver involvement and had bilateral pleural effusion; b. Dengue hemorrhagic grade III with liver involvement and encephalopathy; c. Dengue hemorrhagic grade III with liver involvement acute kidney injury, myocardial involvement and encephalopathy. All the patients were treated according to new edition WHO protocol and all of the involving organ recovered along with the improvement of the disease. CONCLUSION Update management of dengue complication pediatric should be learned carefully used for helping unusual cases of dengue virus infection.

scholarly journals Roles for Endothelial Cells in Dengue Virus Infection

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Nadine A. Dalrymple ◽  
Erich R. Mackow
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Permeability ◽  
Virus Disease ◽  
Dengue Shock Syndrome ◽  
Hemorrhagic Fever ◽  
Dengue Virus Infection ◽  
Barrier Functions ◽  
Dengue Disease ◽  
Primary Fluid

Dengue viruses cause two severe diseases that alter vascular fluid barrier functions, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The endothelium is the primary fluid barrier of the vasculature and ultimately the effects of dengue virus infection that cause capillary leakage impact endothelial cell (EC) barrier functions. The ability of dengue virus to infect the endothelium provides a direct means for dengue to alter capillary permeability, permit virus replication, and induce responses that recruit immune cells to the endothelium. Recent studies focused on dengue virus infection of primary ECs have demonstrated that ECs are efficiently infected, rapidly produce viral progeny, and elicit immune enhancing cytokine responses that may contribute to pathogenesis. Furthermore, infected ECs have also been implicated in enhancing viremia and immunopathogenesis within murine dengue disease models. Thus dengue-infected ECs have the potential to directly contribute to immune enhancement, capillary permeability, viremia, and immune targeting of the endothelium. These effects implicate responses of the infected endothelium in dengue pathogenesis and rationalize therapeutic targeting of the endothelium and EC responses as a means of reducing the severity of dengue virus disease.

scholarly journals GAMBARAN NILAI HEMATOKRIT DAN LAJU ENDAP DARAH PADA ANAK DENGAN INFEKSI VIRUS DENGUE DI MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Sitti S. P. Kamuh ◽  
Arthur E. Mongan ◽  
Maya F. Memah
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Clinical Symptoms ◽  
Clinical Manifestations ◽  
Public Health Problem ◽  
Major Public Health Problem ◽  
Dengue Virus Infection ◽  
Erythrocyte Sedimentation

Abstract: Dengue is a major public health problem that can be found throughout the tropical and subtropical countries around the world. Spectrum of clinical manifestations of dengue virus infection varies greatly. In addition to clinical symptoms, the diagnosis of dengue virus infection need to be supported by blood tests such as hematocrit and erythrocyte sedimentation rate. This study aimed to determine the hematocrit value and erythrocyte sedimentation rate (ESR) in children with dengue virus infection in Manado. This study used a cross-sectional design and was conducted from Desember 2014 to January 2015 at GMIM Pancaran Kasih, Advent, and Robert Wolter Mongisidi hospitals in Manado. There were 37 patients that fulfilled the inclusion criteria, consisted of 17 males and 20 females. The results showed that of 37 patients, there were 36 with hematocrit within normal limits. Moreover, of 37 patients only 6 patients performed the ESR examination; only 1 child had a rapid ESR result (> 15mm/h). Conclusion: In this study, most of the pediatric patients with dengue virus infection in Manado had normal hematocrit. Of 6 patients who had performed ESR test, only 1 had rapid ESR.Keywords: dengue, hematocrit, erythrocyte sedimentation rateAbstrak: Dengue merupakan masalah kesehatan masyarakat yang utama dan dapat ditemui diseluruh daerah tropis dan subtropis di seluruh dunia. Spektrum manifestasi klinis infeksi virus dengue sangat bervariasi. Selain gejala klinis, diagnosis infeksi virus dengue perlu ditunjang hasil uji darah di laboratorium antara lain hematokrit dan laju endap darah (LED). Penelitian ini bertujuan untuk mengetahui nilai hematokrit dan LED pada anak dengan infeksi virus dengue di Manado. Penelitian ini menggunakan desain potong lintang, dilakukan pada bulan Desember 2014 sampai Januari 2015 di RSU GMIM Pancaran Kasih Manado, RS Advent Manado, dan RSAD Robert Wolter Mongisidi Teling17 anak laki-laki dan 20 anak perempuan. Hasil penelitian memperlihatkan 36 dari 37 pasien mempunyai hematokrit normal. Dasri 37 pasien, hanya 6 anak yang menjalani pemeriksaan LED; hanya 1 anak dengan LED cepat (> 15mm/jam). Simpulan: Pada studi ini, sebagian besar pasien anak dengan infeksi virus dengue di Manado mempunyai nilai hematokrit normal. Dari 6 pasien yang dilakukan pemeriksaan LED, 1 anak mempunyai hasil LED cepat.Kata kunci: dengue, hematrokrit, laju endap darah.

scholarly journals Upaya Pencegahan dan Pengendalian Demam Berdarah Dengue dalam Bentuk Peta Tematik di Kecamatan Rappocini

bionature ◽  
2019 ◽  
Vol 19 (2) ◽  
Author(s):  
Suci Wulandhani ◽  
A. Bida Purnamasari ◽  
Ryan Humardani Syam Pratomo
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Hemorrhagic Fever ◽  
Viral Disease ◽  
High Rate ◽  
Dengue Virus Infection ◽  
Dengue Disease ◽  
Keywords Prevention ◽  
Short Time ◽  
And Control

Abstract. Dengue hemorrhagic fever (DHF) is a disease caused by dengue virus infection. One symptom of dengue virus infection is high fever and headache. Dengue virus is a virus from the genus Flavivirus, family of Flaviviridae. This dengue fever is a dangerous viral disease because it can cause sufferers to die in a very short time / several days. The existence and population density are often associated with transmission, endemicity and Extraordinary Events (EE) of DHF. Disease mapping by utilizing digital technology to support epidemiological investigations and also as a tool to monitor regional conditions for dengue disease. The purpose of this study was to determine efforts to prevent and control dengue disease by mapping. The results of the study show that mapping can be seen as increasing and decreasing the number of cases of dengue disease so that it can assist in data management and reporting of information to monitor areas at risk of contracting dengue disease. Suggestions given need to do further research using other variables that are the cause of the high rate of dengue cases, so that it can be utilized by related agencies as the basis of the information system supporting the decision on preventive measures to combat dengue. Keywords: prevention and control, DHF, mapping.

scholarly journals Correlation of Serotype-Specific Dengue Virus Infection with Clinical Manifestations

2012 ◽  
Vol 6 (5) ◽  
pp. e1638 ◽  
Author(s):  
Eric S. Halsey ◽  
Morgan A. Marks ◽  
Eduardo Gotuzzo ◽  
Victor Fiestas ◽  
Luis Suarez ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Clinical Manifestations ◽  
Dengue Virus Infection

scholarly journals Seroprevalence 0f Dengue Virus Infection in Nepal

2013 ◽  
Vol 1 (4) ◽  
pp. 224-227 ◽  
Author(s):  
B P Gupta ◽  
S K Mishra ◽  
K D Manandhar ◽  
R Malla ◽  
C S Tamarakar ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Health Problem ◽  
Public Health Problem ◽  
Cross Sectional Study ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Cross Sectional ◽  
Positive Rate ◽  
History Of

Dengue Virus infection is an emerging mosquito-borne disease. It is a global health problem and its expanding endemicity towards new territories is a serious concern. Relatively a new disease in Nepalese context, dengue abruptly appeared as massive outbreak in 2010, merely four years after its first introduction. It is a nagging public health problem in the low lands of Terai, expanding to new areas of Nepal in recent years.  A cross-sectional study was conducted to determine anti-Dengue IgM positive rate in Lumbini, Dhading and Chitwan district. The study was carried from June 2012 to November 2012. The total number of Serum samples was collected from 275 patients visiting hospitals with history of fever, headache and suspected DF. The samples were examined by ELISA. The anti-Dengue IgM positivity was found to be 29.09 %. The positive rate was highest in Dhading (70.37%) followed by Bharatpur (37.6%) and Lumbini (11.38%). The Dengue positive cases were higher in males (32.5 %) than female (24.8 %). The highest positive cases (41.6%) were from age group less than 15 years. Dengue has substantial expansion in Western and Far Western Terai region of Nepal which was limited to the middle Terai region in the past and mostly infects older people.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9135  Int J Appl Sci Biotechnol, Vol. 1(4): 224-227

scholarly journals Use of Capillary Blood Samples as a New Approach for Diagnosis of Dengue Virus Infection

2007 ◽  
Vol 45 (3) ◽  
pp. 887-890 ◽  
Author(s):  
S. Matheus ◽  
J.-B. Meynard ◽  
V. Lacoste ◽  
J. Morvan ◽  
X. Deparis
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Capillary Blood ◽  
Dengue Virus Infection ◽  
New Approach ◽  
Blood Samples
Sign in / Sign up

Export Citation Format

Share Document