scholarly journals Enhancing Downstream Processing Of Algal-Based Biofuel Using Novel Fused Bacteria And Green Solvents

2021 ◽  
Author(s):  
Asma Fiayaz

The present study investigated the utilization of algal biomass to produce bio-oil and acetone, butanol, and ethanol (ABE) products. Novel Clostridia fusants (C. beijernickii + C. thermocellum-CbCt and C. acetobutylicum + C. thermocellocum-CaCt) were developed using protoplast fusion technique and subsequently subjected to UV radiation for strain enhancement. Resultant mutated fusants showed improvement in thermal stability and higher resistance to biobutanol toxicity. Algal biomass was initially subjected to various hydrolysis treatments prior to fermentation. Combination treatment of thermal, chemical, and enzymatic resulted in maximum sugar release of 27.78 g/L. Maximum biobutanol concentration from fermentation using CbCt resulted in 7.98 g/L. Fermentation using CaCt produced a concentration of 7.39 g/L. Oil extraction from virgin algae investigated a green, bio-based approach using terpenes with ultrasonication and a modified, Bligh and Dyer method, separately. Combination method, ultrasonication followed by the modified Bligh and Dyer, resulted in oil yield of 46.27% (dlimonene) and 39.85% (p-cymene). Oil extraction was also produced from an algae sample following fermentation. Combined extraction method using fermentation sample resulted in oil yield of 65.04%.

2021 ◽  
Author(s):  
Asma Fiayaz

The present study investigated the utilization of algal biomass to produce bio-oil and acetone, butanol, and ethanol (ABE) products. Novel Clostridia fusants (C. beijernickii + C. thermocellum-CbCt and C. acetobutylicum + C. thermocellocum-CaCt) were developed using protoplast fusion technique and subsequently subjected to UV radiation for strain enhancement. Resultant mutated fusants showed improvement in thermal stability and higher resistance to biobutanol toxicity. Algal biomass was initially subjected to various hydrolysis treatments prior to fermentation. Combination treatment of thermal, chemical, and enzymatic resulted in maximum sugar release of 27.78 g/L. Maximum biobutanol concentration from fermentation using CbCt resulted in 7.98 g/L. Fermentation using CaCt produced a concentration of 7.39 g/L. Oil extraction from virgin algae investigated a green, bio-based approach using terpenes with ultrasonication and a modified, Bligh and Dyer method, separately. Combination method, ultrasonication followed by the modified Bligh and Dyer, resulted in oil yield of 46.27% (dlimonene) and 39.85% (p-cymene). Oil extraction was also produced from an algae sample following fermentation. Combined extraction method using fermentation sample resulted in oil yield of 65.04%.


Author(s):  
G. Baskar ◽  
G. Kalavathy ◽  
R. Aiswarya ◽  
I. Abarnaebenezer Selvakumari

Forests ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 212 ◽  
Author(s):  
Yun-Qi Zhang ◽  
Lin Jin ◽  
Jie Duan ◽  
Guo-Chun Zhao ◽  
Yuan-Yuan Xu ◽  
...  

Biodiesel has many advantages, yet its high price has become the main obstacle to market acceptance. Selecting non-edible woody oil plant resources and optimizing the oil extraction process will contribute to the effective utilization of raw materials and development of the related biodiesel industry. This study presents a detailed evaluation of two Sapindus species (Sapindus delavayi (Franch.) Radlk. and Sapindus mukorossi Gaertn.) as promising feedstocks for biodiesel production. As ultrasonic-assisted extraction (UAE) is considered a green and efficient oil extraction method, the process was optimized by response surface methodology (RSM) using a Box–Behnken design (BBD) in our study. The kernel oil yield of S. delavayi was up to 43.67% ± 0.16% under the optimized extraction conditions (the ultrasonic power was 109W, extracting at 65 °C for 25 min, and the liquid–solid ratio was 9 mL·g−1). The kernel oil yield of S. mukorossi was as high as 45.96% ± 0.21% under the optimized extraction conditions (the ultrasonic power was 114W, extracting at 68 °C for 26 min, and the liquid–solid ratio was 9 mL·g−1). The fatty acid profiles of S. delavayi and S. mukorossi kernel oils showed a high percentage of monounsaturated fatty acids (74.91% and 76.32%, respectively) and a low percentage of polyunsaturated fatty acids (11.11% and 7.83%, respectively) and saturated fatty acids (13.98% and 15.85%, respectively). Most of the properties of the two biodiesels conformed to EN 14214:2014, ASTM D6751–2018 and GB 25199–2017 standards, except for oxidation stability. In general, the results provided the optimized extraction method using ultrasound for the two species oil extraction and proved that the two kernel oils are potentially useful feedstocks for high-quality and low-cost biodiesel production.


2020 ◽  
Author(s):  
Eko K. Sitepu ◽  
Andy Chandra ◽  
Emma F. Zaidar ◽  
Annur Vika ◽  
Firman Sebayang ◽  
...  

Abstract Even though the mechanical extraction process offers a simple and environmentally friendly process, the recovery of oil is relatively low. Thermal pre-treating the oilseed increases the oil yield but produces unwanted oil colour. A new method which combines grinding and extraction using green solvents was developed to extract palm kernel oil. The performance of six different green solvents such as water, ethanol, isopropyl alcohol, dimethyl carbonate, ethyl acetate, and d-limonene in extraction palm kernel oil was determined using a controllable blender extractor (CBE), new extraction equipment modified from a household blender appliance. Further, ethyl acetate, which produced the maximum oil yield, was used to study the effect of the operating parameters of the CBE. The oil yield of 34.2 ± 0.02% was obtained in the extraction condition of the ratio of palm kernel to ethyl acetate of 1:7, rotational speed of 5000 rpm and 10 minutes extraction time. Compared to other green extraction methods, the CBE-intensified palm kernel oil extraction could save >70% energy consumption. In terms of extraction time, the CBE-intensified could extract palm kernel oil faster than existing extraction methods.


2020 ◽  
Vol 849 ◽  
pp. 47-52
Author(s):  
Siti Jamilatun ◽  
Aster Rahayu ◽  
Yano Surya Pradana ◽  
Budhijanto ◽  
Rochmadi ◽  
...  

Nowadays, energy consumption has increased as a population increases with socio-economic developments and improved living standards. Therefore, it is necessary to find a replacement for fossil energy with renewable energy sources, and the potential to develop is biofuels. Bio-oil, water phase, gas, and char products will be produced by utilizing Spirulina platensis (SPR) microalgae extraction residue as pyrolysis raw material. The purpose of this study is to characterize pyrolysis products and bio-oil analysis with GC-MS. Quality fuel is good if O/C is low, H/C is high, HHV is high, and oxygenate compounds are low, but aliphatic and aromatic are high. Pyrolysis was carried out at a temperature of 300-600°C with a feed of 50 grams in atmospheric conditions with a heating rate of 5-35°C/min, the equipment used was a fixed-bed reactor. The higher the pyrolysis temperature, the higher the bio-oil yield will be to an optimum temperature, then lower. The optimum temperature of pyrolysis is 550°C with a bio-oil yield of 23.99 wt%. The higher the pyrolysis temperature, the higher the H/C, the lower O/C. The optimum condition was reached at a temperature of 500°C with the values of H/C, and O/C is 1.17 and 0.47. With an increase in temperature of 300-600°C, HHV increased from 11.64 MJ/kg to 20.63 MJ/kg, the oxygenate compound decreased from 85.26 to 37.55 wt%. Aliphatics and aromatics increased, respectively, from 5.76 to 36.72 wt% and 1.67 to 6.67 wt%.


Author(s):  
Thaithat Sudsuansee ◽  
Narong Wichapa ◽  
Amin Lawong ◽  
Nuanchai Khotsaeng

In citronella oil extraction process by steam distillation, inefficient use of steam is the main cause of excessive energy consumption that affects energy cost and oil yield. This research is aimed to reduce the energy cost and increase the oil yield by studying the steam used in the process. The proposed method is the three-stage extraction model combined with the Data Envelopment Analysis developed by Charnes, Cooper and Rhodes (DEA-CCR model). Although the three-stage extraction model has been widely used, there is no research integrate this model with DEA-CCR model. It is well known that DEA-CCR model is an effective tool to evaluate efficiency of decision making units/alternatives. The advantages of this research were presented as the calculation of the optimum distillation conditions, including the steam flow rate and the distillation time, were achieved as discussed in this article. The study was comprised of 3 parts. Firstly, the three-stage extraction model for citronella oil was formulated. Secondly, the results of the proposed model were calculated under different conditions, classified by steam flow rates from 5,000 to 60,000 cm3/min for the distillation period of 15–180 min. Finally, the DEA-CCR model was utilized to evaluate and rank alternatives. The results expressed that the best condition for producing citronella oil was at the steam flow rate of 40,000 cm3/min and the distillation time of 60 min. The optimal energy cost and percentage of oil yield were equal to 0.440 kWh/mL and 0.7%, respectively. When comparing to the experimental results, the percentage error of optimal energy cost and oil yield were slightly different, with a value of 0.98% and 0.85%, respectively. Moreover, the energy consumption was also reduced by 34.6% compared to the traditional operating conditions.


2021 ◽  
Vol 25 (8) ◽  
pp. 1461-1463
Author(s):  
O.E. Ameh ◽  
J.I. Achika ◽  
N.M. Bello ◽  
A.J. Owolaja

This work aimed to extract perfume from the leaves of Cymbopogon citratus using three extraction methods viz: distillation, solvent extraction and expression or effleurage. About 150 g of dried lemons grass were extracted using ethanol as the solvent to obtain essential oils required for the formulation of perfumes. The result of the hydrodistillation process showed that 1.23 g of essential oil per 130g of dry lemongrass produce 0.95% oil at 78 °C, while the effleurage method was 2.55 g of essential oil per 130 g of dry lemongrass sample thereby producing 1.96% oil yield. The solvent extraction method gave 2.7 g of essential oil per 130 g of dry lemongrass sample. This gives about 2.08 % yield of essential oil at a temperature of 78°C i.e. the boiling point of ethanol. The solvent extraction method yielded 2.08%, the effleuragemethod yielded 1.96% and the hydrodistillation method yielded 0.95% essential oil respectively. In conclusion, solvent extraction gave the highest yield because of less exposure to air and heat, which is highly recommended as the most suitable method for the extraction of essential oil.


2020 ◽  
Vol 58 (5) ◽  
pp. 604
Author(s):  
Hong Nam Nguyen ◽  
Bùi Văn Đức ◽  
Ngoc Linh Vu ◽  
Hong Nam Nguyen ◽  
Thi Thu Ha Vu ◽  
...  

Despite its prominent potential, the use of rubber wood (Hevea brasiliensis) for bio-oil production has not been fully investigated. This study reported experimental results of the bio-oil production and upgrading from rubber wood using fast pyrolysis technology. The effects of catalyst nature (vermiculite and dolomite), upgrading temperature and bio-oil/catalyst ratio on the product quality were deeply investigated. The results showed that dolomite was suitable to be used as a catalyst for bio-oil upgrading. At 600 °C and a bio-oil/catalyst ratio of 1:1, the bio-oil yield was maximized, while at 400 °C and a ratio of 1:3, the bio-oil heating value was maximized. Depending on usage purposes, a yield-oriented, heating value-oriented or in-between bio-oil upgrading solution could be considered.


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