Production, Extraction, and Characterization of Green Biodegradable Bioplastics Polyhydroxybutyrates (PHB) for Biomedical Applications

10.32920/ryerson.14640033 ◽

2021 ◽

Author(s):

Jehan Yousif

Keyword(s):

Carbon Footprint ◽

Environmental Conditions ◽

Biomedical Applications ◽

Fossil Fuel ◽

Ralstonia Eutropha ◽

Enzyme Hydrolysis ◽

Chemical Bonds ◽

Hemp Fibers ◽

Simple Sugar

Biodegradable plastics are that kind of plastics that will decompose naturally, when environmental microorganisms metabolize and break down the chemical bonds present in the structure of biopolymer. Bio-plastics offer an advantage to earth by reducing carbon footprint and use of fossil fuel. Bio-plastics are completely biodegradable and can be recycled. The focus of the study was to produce green bioplastics by utilizing the sugar hydrolysate of Hemp fibers. Producing of Bioplastic was pursued in fermentation experiments, where HEMP was utilized as feedstock to produce PHB. Hemp fibers were initially pretreated, and then enzymatically hydrolyzed to produce PHB. Hemp can produce PHB (poly(3-hydroxybutyrate) when the environmental conditions are controlled. The objective of the study was to optimize simple sugar availability from hemp for Ralstonia eutropha. Powedered hemp with the contration of 5% NaOH, and 1.5g/ L of enzyme addition was found to yield the best restluts in the production process of Bioplastics as the fractional insoluble solids (FIS) of ≃ 61 % was significantly better compared to other combinations of pre-treatments studied. The enzyme hydrolysis yield of 10.9 % and PHB yield of ≃ 43 % were also found in this study.

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Production Of Bioplastics From Renewable And Sustainable Feedstock Resources

10.32920/ryerson.14649030 ◽

2021 ◽

Author(s):

Ahmad Chaudhry

Keyword(s):

Ralstonia Eutropha ◽

Ph Control ◽

Enzyme Hydrolysis ◽

Treatment Methods ◽

Hydrolysis Yield ◽

Pre Treatment ◽

Enzyme Dosage ◽

Hydrolysis Conditions ◽

Simple Sugar ◽

Potential Opportunity

This study illustrates the potential opportunity for the utilization of hemp to produce PHB (poly(3-hydroxybutyrate). The objective of the study was to optimize simple sugar availability from hemp for Ralstonia eutropha. The use of three pre-treatment methods (grinded – 5% NaOH – Autoclave at 121 oC for 60 minutes) was able to provide a better fractional insoluble solids (FIS) of ≃ 61 % that was significantly better compared to other combinations of pre-treatments studied. Optimum enzyme dosage was also determined by comparing different enzyme concentrations and found that three enzymes should contain a dose of 1.5 g /L. The optimum pretreatment and hydrolysis conditions resulted in a better enzyme hydrolysis yield of 10.9 % and PHB yield of ≃ 43 %. Results also demonstrate that sonification did not improve PHB recovery, while pH control increased PHB recovery. Keywords: Hemp, Ralstonia eutropha, PHB, Pre-treatment, Enzyme Hydrolysis

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Production Of Bioplastics From Renewable And Sustainable Feedstock Resources

10.32920/ryerson.14649030.v1 ◽

2021 ◽

Author(s):

Ahmad Chaudhry

Keyword(s):

Ralstonia Eutropha ◽

Ph Control ◽

Enzyme Hydrolysis ◽

Treatment Methods ◽

Hydrolysis Yield ◽

Pre Treatment ◽

Enzyme Dosage ◽

Hydrolysis Conditions ◽

Simple Sugar ◽

Potential Opportunity

This study illustrates the potential opportunity for the utilization of hemp to produce PHB (poly(3-hydroxybutyrate). The objective of the study was to optimize simple sugar availability from hemp for Ralstonia eutropha. The use of three pre-treatment methods (grinded – 5% NaOH – Autoclave at 121 oC for 60 minutes) was able to provide a better fractional insoluble solids (FIS) of ≃ 61 % that was significantly better compared to other combinations of pre-treatments studied. Optimum enzyme dosage was also determined by comparing different enzyme concentrations and found that three enzymes should contain a dose of 1.5 g /L. The optimum pretreatment and hydrolysis conditions resulted in a better enzyme hydrolysis yield of 10.9 % and PHB yield of ≃ 43 %. Results also demonstrate that sonification did not improve PHB recovery, while pH control increased PHB recovery. Keywords: Hemp, Ralstonia eutropha, PHB, Pre-treatment, Enzyme Hydrolysis

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P.1-6 Synthesis and structural properties characterization of Ap/MgO nanocomposites for biomedical applications.

10.35841/biology-medicine.1000101 ◽

2017 ◽

Vol 01 (01) ◽

Author(s):

Vijayalakshmi V ◽

Dhanasekaran P

Keyword(s):

Structural Properties ◽

Biomedical Applications ◽

Properties Characterization

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Synthesis and structural properties characterization of Ap/MgO nanocomposites for biomedical applications.

10.35841/biology-medicine.1.1.1-6 ◽

2017 ◽

Vol 01 (01) ◽

Author(s):

Vijayalakshmi V ◽

Dhanasekaran P

Keyword(s):

Structural Properties ◽

Biomedical Applications ◽

Properties Characterization

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Optimization and characterization of flavonoids extracted from Cannabis sativa fibers

Textile Research Journal ◽

10.1177/00405175211027796 ◽

2021 ◽

pp. 004051752110277

Author(s):

Qilu Cui ◽

Jiawei Li ◽

Chongwen Yu

Keyword(s):

Chemical Structure ◽

Cannabis Sativa ◽

Ethanol Concentration ◽

Extraction Process ◽

Test Results ◽

Hemp Fiber ◽

Hemp Fibers ◽

Structure Surface ◽

Degumming Process

In this paper, the extraction process of flavonoids from hemp fibers was studied. Response surface methodology (RSM) analysis of the extraction parameters indicated that optimized results would be ethanol concentration 76 vol.%, bath ratio 1:50, and reaction time 139 min; therefore, an optimal extraction rate of flavonoids of 0.2275% can be obtained. The chemical structure, surface morphology and element composition of flavonoid extracts were analyzed. The test results indicated that hemp extract contains flavonoids, which can be used to extract flavonoids from hemp fiber, so as to comprehensively develop hemp fiber and reduce the discharge of waste liquid in the traditional degumming process.

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Preparation and Characterization of Chitosan-Based Scaffolds for Biomedical Applications

Materials Science Forum ◽

10.4028/www.scientific.net/msf.514-516.1005 ◽

2006 ◽

Vol 514-516 ◽

pp. 1005-1009 ◽

Cited By ~ 2

Author(s):

José V. Araújo ◽

J.A. Lopes da Silva ◽

Margarida M. Almeida ◽

Maria Elisabete V. Costa

Keyword(s):

Biomedical Applications ◽

Freeze Drying ◽

Average Pore Size ◽

Composite Scaffolds ◽

Average Pore ◽

Chitosan Matrix ◽

Freeze Dried ◽

Porous Chitosan ◽

Interconnected Structure

Porous chitosan/brushite composite scaffolds were prepared by a freeze-drying technique, starting from brushite suspensions in chitosan solutions. The obtained scaffolds showed a regular macroporous and interconnected structure with brushite particles uniformly distributed in the chitosan matrix. The variation of the brushite concentration affected the microstructure of the final freeze-dried scaffold, in particular, its porosity and its average pore size. The yield strengths of the composite scaffolds could also be improved by the increase of the brushite content.

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Synthesis and characterization of novel protein nanodots as drug delivery carriers with an enhanced biological efficacy of melatonin in breast cancer cells

RSC Advances ◽

10.1039/d0ra08959a ◽

2021 ◽

Vol 11 (16) ◽

pp. 9076-9085

Author(s):

Kanchan Yadav ◽

Megha Das ◽

Nurul Hassan ◽

Archana Mishra ◽

Jayeeta Lahiri ◽

...

Keyword(s):

Breast Cancer ◽

Drug Delivery ◽

Cancer Cells ◽

Live Cell Imaging ◽

Biomedical Applications ◽

Breast Cancer Cells ◽

Cell Imaging ◽

Synthesis And Characterization ◽

Novel Protein

A novel nanodot-using protein has been synthesized for the live cell imaging and drug delivery of melatonin in breast cancer cells. Its unique properties hold potential for various biomedical applications in the field of bioimaging and drug delivery.

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