scholarly journals Growth Inhibition of Phytopathogenic Fungi and Oomycetes by Basidiomycete Irpex lacteus and Identification of its Antimicrobial Extracellular Metabolites

2021 ◽  
Vol 70 (1) ◽  
pp. 131-136
Author(s):  
DAISY PINEDA-SUAZO ◽  
JOSAPHAT MIGUEL MONTERO-VARGAS ◽  
JOSÉ JUAN ORDAZ-ORTIZ ◽  
GERARDO VÁZQUEZ-MARRUFO
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Irpex Lacteus ◽  
Phytophthora Spp ◽  
Extracellular Metabolites ◽  
Colletotrichum Spp

In dual culture confrontation assays, basidiomycete Irpex lacteus efficiently antagonized Fusarium spp., Colletotrichum spp., and Phytophthora spp. phytopathogenic strains, with growth inhibition percentages between 16.7–46.3%. Antibiosis assays evaluating the inhibitory effect of soluble extracellular metabolites indicated I. lacteus strain inhibited phytopathogens growth between 32.0–86.7%. Metabolites in the extracellular broth filtrate, identified by UPLC-QTOF mass spectrometer, included nine terpenes, two aldehydes, and derivatives ofa polyketide, a quinazoline, and a xanthone, several of which had antifungal activity. I. lacteus strain and its extracellular metabolites might be valuable tools for phytopathogenic fungi and oomycete biocontrol of agricultural relevance.

scholarly journals Characterization of the antifungal activity of three rhizobacterial strains against Rhizoctonia solani

2019 ◽  
Vol 16 (4) ◽  
pp. e1011
Author(s):  
Deisy Y. Pineda-Mendoza ◽  
Apolinar González-Mancilla ◽  
Juan J. Almaraz ◽  
María P. Rodríguez-Guzmán ◽  
Oscar García-Barradas ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Rhizoctonia Solani ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Dual Culture ◽  
Potato Dextrose Broth ◽  
Potato Dextrose Agar Medium ◽  
Fungal Inhibition ◽  
The One

 In this study, three rhizobacterial strains were characterized by their ability to inhibit Rhizoctonia solani and tested in chili (Capsicum annuum L.) seedlings. Strains A46 and P61 were identified as Pseudomonas tolaasii, and S108 as Rhanella aquatilis. In the dual culture tests, all the strains inhibited the radial growth of R. solani. None of the three strains produced chitinases or volatile compounds, but they were found to produce siderophores. However, this last characteristic was not responsible for the rhizobacterial inhibitory effect on the growth of R. solani, other bacterial metabolites were possibly involved. The bacterial filtrates added to the potato dextrose agar medium (PDA) in a 3:7 ratio had significant antifungal activity, being the filtrate from strain S108 the one that showed the highest effect, with 56% fungal inhibition. The co-culture of strain A46 with the phytopathogenic fungi in potato dextrose broth (PDB) increased the antifungal activity of the rhizobacterial filtrate. The application of the rhizobacterial strains to Serrano chili decreased the R. solani-related mortality rate in seedlings; particularly, S108 had the greatest effect, which was similar to the fungicide effect. This study showed that the S108 strain has potential as a biofungicide to control R. solani in chili seedlings.

scholarly journals Growth inhibition of some Eurotium and Aspergillus species with spice extracts

2007 ◽  
pp. 63-70 ◽  
Author(s):  
Gordana Dimic ◽  
Suncica Kocic-Tanackov ◽  
Dragana Karalic
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Aspergillus Flavus ◽  
Inhibitory Effect ◽  
Seed Extract ◽  
Aspergillus Species ◽  
Micellar Growth ◽  
Test Cultures

The antifungal activity of spice extracts obtained from caraway seed, garlic and origanum was tested against antifungal activity of Eurotium herbariorum E. amstelodami, Aspergillus flavus and A. sydowii. Caraway seed extract has been proved to possess the highest inhibitory effect on all investigated mould species. The concentrations of caraway extract, sufficient to inhibit the growth completely were: 0,5% for E. herbariorum, E. amstelodami and A. sydowii, and 1% for A. flavus; of garlic, 1% for Eurotium spp. and 2% for A. sydowii, and of origanum, 1% for E. herbariorum and 2% for E. amstelodami. The results of colonies diameter measuring showed that garlic and origanum extracts have no significant suppressing ability on micellar growth of A. flavus, while garlic was more efficient in other test cultures.

Antifungal Activity of Oosporein from an Antagonistic Fungus against Phytophthora infestans

2004 ◽  
Vol 59 (3-4) ◽  
pp. 302-304 ◽  
Author(s):  
Toshinori Nagaoka ◽  
Kengo Nakata ◽  
Kenji Kouno
Keyword(s):  
Antifungal Activity ◽  
Phytophthora Infestans ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Growth Inhibitory Effect ◽  
Antifungal Metabolite ◽  
Antagonistic Effects ◽  
Significant Growth ◽  
Growth Inhibitory

An antifungal metabolite, oosporein, was isolated from the culture of Verticillium psalliotae that produced the antagonistic effects on Phytophthora infestans. Oosporein exhibited a significant growth-inhibitory effect on P. infestans in comparison with other phytopathogenic fungi.

scholarly journals The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Qonita Gina Fadhilah ◽  
Iman Santoso ◽  
Yasman YASMAN
Keyword(s):  
Growth Inhibition ◽  
Incubation Period ◽  
Culture Method ◽  
Mangrove Ecosystem ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Marine Actinomycetes ◽  
Mangrove Ecosystems ◽  
Incubation Periods

Abstract. Fadhilah QG, Santoso I, Yasman. 2021. The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA. Biodiversitas 22: 642-649.  Marine actinomycetes from mangrove ecosystems are known to be potential antifungal-producing isolates against phytopathogenic fungi. The aim of this research was to obtain potential marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum sp. KA. Screening of 15 marine actinomycetes isolates using a dual culture method with a plug technique showed that 80% of isolates have antagonistic activity, represented as a percentage of growth inhibition range from 47.96% to 84.94%. Among 12 potential isolates, six isolates (SM4, SM11, SM14, SM15, SM18, and SM20) were evaluated for delayed antagonistic activity with incubation periods of 6, 9, and 12 days using the plug and streak techniques. The results showed that the percentage of growth inhibition of selected isolates inclined to increase along with the incubation period prior to inoculation of Colletotrichum sp. KA. Delayed antagonist assays using the streak technique resulted in higher inhibition results compared to the plug technique. Furthermore, the non-delayed assays of the two selected isolates, SM11 and SM15, also inhibited Colletotrichum sp. KA 57.99% and 59.88%, respectively. The delayed antagonist assay with a shorter incubation period of the two selected isolates also showed an increased percentage of growth inhibition of Colletotrichum sp. KA. According to our research, the delayed antagonistic assay of marine actinomycetes isolates with a 12-day incubation period using a plug technique was representative to evaluate the percentage of growth inhibition.

scholarly journals Antifungal activity of some botanical extracts on Fusarium oxysporum

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Domenico Rongai ◽  
Patrizio Pulcini ◽  
Barbara Pesce ◽  
Filomena Milano
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Titratable Acidity ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Conidia Germination

AbstractThe present paper describes the antifungal activity of some plant extracts on the development of Fusarium oxysporum f.sp. lycopersici. The best extracts were selected to be tested as a phytofungicide to control crop diseases, with the ultimate goal of developing a green alternative to synthetic fungicides. Using the conidia germination assay, of the 24 plant extracts tested, 15 reduced conidia germination and 6 completely inhibited germination. Extracts of Rivina humulis, Brassica carinata, Brunfelsia calyicina, Salvia guaranitica and Punica granatum showed good antifungal activity. The relationship between total phenolic content (TPC) in each plant extract tested and the percentage of mycelial growth inhibition showed a significant correlation (R2 = 0.69), while no correlation was found between total flavonoid content (TFC) and percentage mycelial growth inhibition. Among all extracts tested, Punica granatum and Salvia guaranitica showed the best inhibitory effect against Fusarium oxysporum f.sp. lycopersici . Our results indicate that plant extracts with a good antifungal activity generally had a high level of total polyphenolic content and titratable acidity, and low values of pH.

scholarly journals Chemical composition and in vitroantifungal activity of turmeric oilfrom Curcuma longa L. rhizomeagainst Colletotrichum spp.

2021 ◽  
Vol 63 (6) ◽  
pp. 6-10
Author(s):  
Trung Hieu Pham ◽  
◽  
Dai Lam Tran ◽  
Thi Nam Pham ◽  
Dinh Hoang Vu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Colletotrichum Gloeosporioides ◽  
Curcuma Longa ◽  
Inhibitory Effect ◽  
Volatile Components ◽  
In Vitro Growth ◽  
Strong Inhibition ◽  
Colletotrichum Spp ◽  
Poisoned Food Technique

The production of curcumin produces large quantities of the spent turmeric oleoresin as a by-product, which often pollutes the environment. This study was conducted to obtain turmeric oil from this by-product and evaluate its antifungal activity. Turmeric oil afforded by partitioning the turmeric oleoresin from the production of curcumin (Curcuma longa L.) with n-hexane showed a strong inhibition for Colletotrichum species. The volatile components of turmeric oil were determined by GC-MS. Then, 23 volatile compounds were identified from this oil, of which four main compounds were α-zingiberene, α-turmerone, ar-turmerone, and β-sesquiphellandrene. Besides, the authors also isolated Colletotrichum gloeosporioides from lychee fruits, which were harvested in Luc Ngan district, Bac Giang province. By poisoned food technique, the antifungal activity of turmeric oil against C. gloeosporioides, C. orbiculare, C. acutatum, Phytophthora infestans, and Fusarium oxysporum was demonstrated. The results showed that at a concentration of 1 mg/ml, turmeric oil showed the highest inhibitory effect on C. gloeosporioides (67.9%). As for other fungi, in vitro growth inhibition varied in the range of 41-62%.

scholarly journals Antifungal Activity of Earthworm Coelomic Fluid Obtained from Eisenia andrei, Dendrobaena veneta and Allolobophora chlorotica on Six Species of Phytopathogenic Fungi

Environments ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 102
Author(s):  
Sandra Ečimović ◽  
Karolina Vrandečić ◽  
Martina Kujavec ◽  
Martina Žulj ◽  
Jasenka Ćosić ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Crop Protection ◽  
Macrophomina Phaseolina ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Coelomic Fluid ◽  
Economic Damage ◽  
Eisenia Andrei ◽  
Earthworm Species ◽  
Allolobophora Chlorotica

The functioning of soil ecosystems greatly depends on the interactions occurring between soil biota communities. It is well known that earthworms are an important soil component that substantially affects its function, including their meaningful impact on the development of different phytopathogenic soil fungi. Phytopathogenic fungi are responsible for crop disease and cause great economic damage. It has previously been established that earthworms’ coelomic fluid can suppress the growth of phytopathogenic fungi, but the exact molecular mechanism is unknown. The present study aimed at broadening the proof of this observed phenomenon by investigating the effects of the coelomic fluid extract of three different earthworm species (Eisenia andrei, Dendrobaena veneta and Allolobophora chlorotica) on the growth of six different phytopathogenic fungi species (Berkeleyomyces basicola, Fusarium culmorum, Globisporangium irregulare, Rhizoctonia solani, Macrophomina phaseolina, and Sclerotinia sclerotiorum). Coelomic fluid extract was obtained by electrostimulation or usage of extraction buffer (only in case of A. chlorotica) and prepared in three different concentrations by diluting the obtained coelomic fluid with physiological saline. The coelomic fluid extract of the three investigated earthworm species had an inhibitory effect on the growth of all six phytopathogenic fungi species. The greatest inhibitory effect was achieved with the E. andrei coelomic fluid extract reducing the growth of R. solani fungi. The findings of this research confirm the antifungal activity of coelomic fluid obtained from earthworm species belonging to different ecological categories and may be of potential use in crop protection against phytopathogenic fungi.

Characteristics and Function of the Chitin Binding Protein from Xenorhabdus nematophila

2019 ◽  
Vol 26 (6) ◽  
pp. 414-422
Author(s):  
Jia Liu ◽  
Ping Song ◽  
Jie Zhang ◽  
Ziyan Nangong ◽  
Xiaobei Liu ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Insecticidal Activity ◽  
Spore Germination ◽  
Binding Protein ◽  
Colloidal Chitin ◽  
Inhibition Rate ◽  
Xenorhabdus Nematophila ◽  
Chitin Binding Protein ◽  
And Function

Background: Genome sequence analysis (GenBank access No.: FN667742.1) shows that Xenorhabdus nematophila ATCC19061 contains one gene (Xn-cbp) encoding chitin binding protein (Xn-CBP). Objective: The present work aims to clarify the characteristics and function of Xn-CBP from X. nematophila HB310. Methods: In this study, the Xn-cbp gene was cloned and expressed in Escherichia coli BL21 (DE3). Substrate binding assays were performed to explain the ability of Xn-CBP combined with the polysaccharide. The insecticidal toxicity of Xn-CBP against the second-instar larvae of Helicoverpa armigera was determined by feeding method. Besides, the antifungal activity of Xn-CBP against Coniothyrium diplodiella, Verticillium dahlia, and Fusarium oxysporum was tested by spore germination assay and hyphal extension assay. Results: Xn-CBP encoded 199 amino acids with a calculated mass of 28 kDa, which contained a signal peptide and a chitin binding domain. The Bmax and Kd values of Xn-CBP to colloidal chitin were 2.46 and 4.08, respectively. Xn-CBP had insecticidal activity against the H. armigera with a growth inhibition rate of 84.08%. Xn-CBP had the highest spore germination inhibitory effect on C. diplodiella with the inhibition rate of 83.11%. The hyphal growth inhibition rate of Xn-CBP to F. oxysporum, 41.52%, was higher than the other two fungi. Conclusion: The Xn-CBP had the highest binding ability to colloidal chitin and it showed insecticidal activity and antifungal activity. The present study laid a foundation for further exploitation and utilization of X. nematophila.

scholarly journals Stage-dependent effect of deferoxamine on growth of Plasmodium falciparum in vitro

Blood ◽  
1990 ◽  
Vol 76 (6) ◽  
pp. 1250-1255 ◽  
Author(s):  
S Whitehead ◽  
TE Peto
Keyword(s):  
Plasmodium Falciparum ◽  
Growth Inhibition ◽  
Antimalarial Activity ◽  
Clinical Malaria ◽  
Inhibitory Effect ◽  
Parasite Development ◽  
And Control ◽  
Speed Of Onset

Abstract Deferoxamine (DF) has antimalarial activity that can be demonstrated in vitro and in vivo. This study is designed to examine the speed of onset and stage dependency of growth inhibition by DF and to determine whether its antimalarial activity is cytostatic or cytocidal. Growth inhibition was assessed by suppression of hypoxanthine incorporation and differences in morphologic appearance between treated and control parasites. Using synchronized in vitro cultures of Plasmodium falciparum, growth inhibition by DF was detected within a single parasite cycle. Ring and nonpigmented trophozoite stages were sensitive to the inhibitory effect of DF but cytostatic antimalarial activity was suggested by evidence of parasite recovery in later cycles. However, profound growth inhibition, with no evidence of subsequent recovery, occurred when pigmented trophozoites and early schizonts were exposed to DF. At this stage in parasite development, the activity of DF was cytocidal and furthermore, the critical period of exposure may be as short as 6 hours. These observations suggest that iron chelators may have a role in the treatment of clinical malaria.

Sign in / Sign up

Export Citation Format

Share Document