scholarly journals The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA

2021 ◽  
Vol 22 (2) ◽  
Author(s):  
Qonita Gina Fadhilah ◽  
Iman Santoso ◽  
Yasman YASMAN
Keyword(s):  
Growth Inhibition ◽  
Incubation Period ◽  
Culture Method ◽  
Mangrove Ecosystem ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Marine Actinomycetes ◽  
Mangrove Ecosystems ◽  
Incubation Periods

Abstract. Fadhilah QG, Santoso I, Yasman. 2021. The antagonistic activity of marine actinomycetes from mangrove ecosystem against phytopathogenic fungi Colletotrichum sp. KA. Biodiversitas 22: 642-649.  Marine actinomycetes from mangrove ecosystems are known to be potential antifungal-producing isolates against phytopathogenic fungi. The aim of this research was to obtain potential marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum sp. KA. Screening of 15 marine actinomycetes isolates using a dual culture method with a plug technique showed that 80% of isolates have antagonistic activity, represented as a percentage of growth inhibition range from 47.96% to 84.94%. Among 12 potential isolates, six isolates (SM4, SM11, SM14, SM15, SM18, and SM20) were evaluated for delayed antagonistic activity with incubation periods of 6, 9, and 12 days using the plug and streak techniques. The results showed that the percentage of growth inhibition of selected isolates inclined to increase along with the incubation period prior to inoculation of Colletotrichum sp. KA. Delayed antagonist assays using the streak technique resulted in higher inhibition results compared to the plug technique. Furthermore, the non-delayed assays of the two selected isolates, SM11 and SM15, also inhibited Colletotrichum sp. KA 57.99% and 59.88%, respectively. The delayed antagonist assay with a shorter incubation period of the two selected isolates also showed an increased percentage of growth inhibition of Colletotrichum sp. KA. According to our research, the delayed antagonistic assay of marine actinomycetes isolates with a 12-day incubation period using a plug technique was representative to evaluate the percentage of growth inhibition.

scholarly journals Actinomycetes of Juniperus excelsa Bield. rhizosphere – antagonists of phytopathogenic microbiota

2018 ◽  
Vol 23 ◽  
pp. 340-345
Author(s):  
S. I. Tistechok ◽  
V. Ya. Syrvatka ◽  
V. O. Fedorenko ◽  
O. M. Gromyko
Keyword(s):  
Pathogenic Bacteria ◽  
Culture Method ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Productivity Losses ◽  
Microbiological Methods ◽  
Juniperus Excelsa ◽  
Bacteria And Fungi ◽  
Phytopathogenic Microorganisms

Aim. Phytopathogenic microorganisms are one of the main causes of agricultural productivity losses. Thereby, the goal of this study was to evaluate actinomycetes strains, isolated from Juniperus excelsa Bield. rhizosphere, antagonistic activity against plant pathogenic bacteria and fungi. Methods. In this study we used microbiological methods for isolation actinomycetes from rhizosphere. Antagonistic activity was evaluated by using the dual culture method. Results. 372 actinomycete stains were isolated from J. excelsa Bield. rhizosphere. More than 60 % actinomyces isolates showed antibacterial activity against to lest one of the tested phytopathogenic bacteria genus Pseudomonas, Pectobacterium, Agrobacterium, Erwinia, Xanthomonas and 20.5 % of the tested phytopathogenic fungi genus Aspergillus, Alternaria, Fusarium, Botrytis. Only 2 strains had antagonistic activity to the all of the tested microorganisms and 62 strains, which had antagonistic activity to the one test-microorganism. Conclusions. Actinomicetes of J. excelsa Bield. rhizosphere are source for bioactive compounds against phytopatogenic microorganisms and showed good biotechnology potential. These results are the first step to the screening new biopesticides for controlling phytopatogenic diseases in plan. Keywords: actynomicetes, phytopathogens, biocontrol.

scholarly journals Evaluation of the antifungal activity of marine actinomycetes isolates against the phytopathogenic fungi Colletotrichum siamense KA: A preliminary study for new antifungal compound discovery

Pharmacia ◽  
2021 ◽  
Vol 68 (4) ◽  
pp. 837-843
Author(s):  
Qonita Gina Fadhilah ◽  
Iman Santoso ◽  
Andi Eko Maryanto ◽  
Sarini Abdullah ◽  
Yasman Yasman
Keyword(s):  
Antifungal Activity ◽  
Mangrove Ecosystem ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Antifungal Compound ◽  
Antifungal Compounds ◽  
Marine Actinomycetes ◽  
Colletotrichum Siamense ◽  
Preliminary Study ◽  
Different Strains

Marine actinomycetes are being explored to discover potential actinomycetes that produce antifungal compounds. In a previous study, marine actinomycetes isolates from the mangrove ecosystem were found to inhibit growth of the phytopathogenic fungi Colletotrichum siamense KA. In this study, the three of these isolates with the highest antagonistic activity—SM11, SM14, and SM15—were evaluated for their antifungal activity using antibiosis assay. The fermentation was performed in SCB:PDB medium (1:1) for 6, 9, and 12 days. The results showed that SM14 was the strongest potential isolate; it inhibited the growth of C. siamense KA on average up to 64.90% for 12 days on PDA filtrate medium. Molecular identification showed SM14 was closely related to Streptomyces sanyensis, but had differences in morphological and biochemical characteristics compared to SM11 or SM15. This indicated that the three isolates were different strains and may challenge further research on identifying and analyzing their antifungal compounds.

scholarly journals Penapisan Cendawan Trichoderma spp. untuk Pengendalian Phytophthora infestans secara in vitro

2016 ◽  
Vol 12 (3) ◽  
pp. 96
Author(s):  
Susiana Purwantisari ◽  
Achmadi Priyatmojo ◽  
Retno Peni Sancayaningsih ◽  
Rina Sri Kasiamdari
Keyword(s):  
Growth Inhibition ◽  
Phytophthora Infestans ◽  
Pathogenic Fungus ◽  
Culture Method ◽  
Inhibition Zone ◽  
Dual Culture ◽  
Trichoderma Spp ◽  
Suppressive Soil ◽  
Blight Disease

Late blight disease on potato caused by a plant pathogenic fungus (Phytophthora infestans)  is the most important disease in Indonesia.  The use of antagonist fungi Trichoderma is an environmentally friendly technology to control the potato disease. The purpose of this study was to determine the ability of Trichoderma spp. to control P. infestans in vitro. Trichoderma spp. have been isolated from suppressive soil at central potato areas in district of Lembang, Bandung, West Java Province. To determine the inhibition ability of Trichoderma spp against P. infestans, a dual culture method was performed.  Variable observed was inhibition zone of Trichoderma spp. against P. infestans. The results showed that 9 isolates of Trichoderma were successfuly isolated from suppressive soil, i.e. T. viride (2 isolates), T. atroviride (1 isolate), T. harzianum (1 isolate) and T. aureoviride (5 isolates). All the Trichoderma isolates revealed growth inhibition abality against P. infestans and. The highest growth inhibition (68.6%) was observed by T. viride isolate ( Ti 9).

scholarly journals Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 891
Author(s):  
Mila Santos ◽  
Fernando Diánez ◽  
Alejandro Moreno-Gavíra ◽  
Brenda Sánchez-Montesinos ◽  
Francisco J. Gea
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

scholarly journals Growth Inhibition of Phytopathogenic Fungi and Oomycetes by Basidiomycete Irpex lacteus and Identification of its Antimicrobial Extracellular Metabolites

2021 ◽  
Vol 70 (1) ◽  
pp. 131-136
Author(s):  
DAISY PINEDA-SUAZO ◽  
JOSAPHAT MIGUEL MONTERO-VARGAS ◽  
JOSÉ JUAN ORDAZ-ORTIZ ◽  
GERARDO VÁZQUEZ-MARRUFO
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Inhibitory Effect ◽  
Phytopathogenic Fungi ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Irpex Lacteus ◽  
Phytophthora Spp ◽  
Extracellular Metabolites ◽  
Colletotrichum Spp

In dual culture confrontation assays, basidiomycete Irpex lacteus efficiently antagonized Fusarium spp., Colletotrichum spp., and Phytophthora spp. phytopathogenic strains, with growth inhibition percentages between 16.7–46.3%. Antibiosis assays evaluating the inhibitory effect of soluble extracellular metabolites indicated I. lacteus strain inhibited phytopathogens growth between 32.0–86.7%. Metabolites in the extracellular broth filtrate, identified by UPLC-QTOF mass spectrometer, included nine terpenes, two aldehydes, and derivatives ofa polyketide, a quinazoline, and a xanthone, several of which had antifungal activity. I. lacteus strain and its extracellular metabolites might be valuable tools for phytopathogenic fungi and oomycete biocontrol of agricultural relevance.

scholarly journals ANTAGONISTIC TEST OF RIAU LOCAL FUNGAL ISOLATES AGAINST SOME PATHOGENIC IN CULTIVATED PLANTS

2019 ◽  
Vol 12 (2) ◽  
pp. 124-132
Author(s):  
Nia Safitri ◽  
Atria Martina ◽  
Rodesia Mustika Roza
Keyword(s):  
Fusarium Oxysporum ◽  
Crop Production ◽  
Plant Pathogens ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Cultivated Plants ◽  
Dual Culture ◽  
Trichoderma Sp

Tanaman budi daya merupakan tanaman yang sering diserang oleh cendawan pathogen, sehingga mengakibatkan penurunan populasi dan produksi tanaman. Pengendalian hayati dengan cendawan antagonis merupakan salah satu metode yang paling efektif dan lebih ramah lingkungan dalam menekan pertumbuhan patogen tanaman. Penelitian ini bertujuan untuk menguji aktivitas antagonis cendawan isolat lokal Riau terhadap beberapa cendawan patogen pada tanaman budi daya. Uji antagonis dilakukan secara in vitro dengan metode dual culture menggunakan lima belas cendawan isolat lokal Riau terhadap Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus dan Colletotrichum sansevieria. Hasil penelitian menunjukkan bahwa Trichoderma sp. PNE 4 memiliki aktivitas antagonis tertinggi dan isolat FER C1 serta isolat LLB07 hanya memiliki aktivitas antagonis yang tinggi dalam menekan pertumbuhan cendawan patogen. Trichoderma sp. PNE 4 mampu menghambat pertumbuhan miselium F. oxysporum sebesar 85,30%, G. Philippii (100%), G. boninense (100%), dan C. sansevieria (100%). Isolat FER C1 hanya menghambat R. Microporus (50,39%) dan isolat LLB07 menghambat G. philippii (52,20%). Trichoderma sp. PNE 4 merupakan cendawan uji yang terpilih sebagai cendawan antagonis, karena memiliki kemampuan daya hambat  >70%.Abstract Cultivated plants are often attacked by pathogenic fungi resulting in a decline of population and crop production. Biocontrol with antagonistic fungi is one of the most effective and environmentally friendly methods in suppressing the growth of plant pathogens. This study aims to examine the antagonistic activity of local isolates fungi Riau against some pathogenic fungi on cultivated plants. The antagonistic test was performed in vitro by dual culture method using fifteen local isolates fungal Riau against Fusarium oxysporum f.sp. lycopersici, Ganoderma philippii, G. boninense, Rigidoporus microporus and Colletotrichum sansevieria. The results showed that Trichoderma sp. PNE 4 isolate exhibited highest activites and  FER C1 and LLB07 isolates exhibited high activities suppressed the growth of the fungal pathogen. Trichoderma sp. PNE 4 isolate inhibited mycelial growth F. oxysporum (85.30%), G. philippii (100%), G. boninense (100%) and C. sansevieria (100%). FER C1 isolate only inhibited R. microporus (50.39%), and LLB07 isolate inhibited G. philippii (52.20%). Trichoderma sp. PNE 4 isolate is test isolates as fungal antagonistic.

scholarly journals Bioactivity of Trichoderma harzianum isolates against the fungal root rot pathogens with special reference to Macrophomina phaseolina causing dry root rot of mungbean

2020 ◽  
Vol 73 (4) ◽  
pp. 787-792
Author(s):  
Alyssa Swehla ◽  
Abhay K. Pandey ◽  
Ramakrishnan M. Nair
Keyword(s):  
Growth Inhibition ◽  
Trichoderma Harzianum ◽  
Root Rot ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Macrophomina Phaseolina ◽  
Antagonistic Activity ◽  
Culture Technique ◽  
Dual Culture ◽  
Fallow System

AbstractIn the rice-fallow system, dry root rot (DRR) is an emerging disease of mungbean (Vigna radiata (L.) R. Wilczek var. radiata) caused by the necrotrophic fungus Macrophomina phaseolina. The pathogen causes extensive production losses. In this study, the bioactivity of four Trichoderma harzianum isolates, namely Th-Dharwad, Th-Raichur, Th-Niphm, and Th-Udaipur procured from the Indian research institutes were evaluated against M. phaseolina of mungbean by the dual culture technique. The efficacy of these T. harzianum isolates were also compared with the effective fungicides such as thiram and carbendazim by the poison food method. Results showed that among the T. harzianum isolates, isolate of Th-Raichur was most effective, exhibiting 76.96% mycelial growth inhibition of the test pathogen. As compared to the thiram, carbendazim was more effective, and exhibited 100% mycelial growth inhibition of the test pathogen. In addition, carbendazim was also more effective than the isolate of Th-Raichur. In the sick pot experiment, mungbean seeds treated with Th-Raichur isolate showed a lower percent incidence of DRR (20%) than the untreated seeds (86.6%). The biological spectrum of Th-Raichur isolate was examined against M. phaseolina isolated from the different hosts such as urdbean and vegetable soybean, alongwith two other root pathogens, namely Fusarium solani of mungbean, and Sclerotium rolfsii of urdbean. The isolate of Th-Raichur showed maximum antagonistic activity against the pathogens M. phaseolina and F. solani of mungbean. Thus, Th-Raichur isolate can be used as a potential fungal biocontrol agent for the reduction of DRR in mungbean.

scholarly journals Evaluation of Antifungal Activity of Antagonistic Bacteria against Butt Rot Disease Pathogen of Pineapple

2017 ◽  
Vol 15 (4) ◽  
pp. 283-293 ◽  
Author(s):  
Mat Nawi NURNADIRAH ◽  
Kartini Che Mohd Ramli NENI ◽  
Mohd Yunus Nor YUZIAH
Keyword(s):  
Antifungal Activity ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Butt Rot ◽  
Additional Control ◽  
Inhibitory Potential ◽  
Rot Disease

Butt rot disease, caused by Thielaviopsis paradoxa (De Seynes) Hohn., is one of the major diseases in pineapple cultivation in Malaysia. The objectives of this study were to evaluate the antifungal effect of antagonist bacteria against T. paradoxa, a causal agent of butt rot disease, and to observe the mechanism of antifungal activity of tested antagonist bacteria microscopically. In this study, in vitro antifungal potential of 5 antagonist bacteria, namely B1, B2, B3, B4, and B5, were isolated from infected and non-infected soil samples and evaluated using dual culture method against T. paradoxa. The mechanisms of antifungal activities of antagonist bacteria against the pathogen were microscopically observed. All of the bacteria showed inhibitory effects against the pathogenic fungi. B1 bacteria showed the highest inhibitory potential, with 73 % inhibition, followed by B2, B3, B4, and B5, with 71, 57, 56, and 48 % of inhibition compared to control, respectively. The results also showed that B2, B3, and B4 bacteria exhibited positive inhibition towards the pathogen, with more than 50 % percentage inhibition. The development of a new product for use as a biocontrol agent, used as an additional control or used in combination with existing ones, may reduce dependency on chemical control and increase antagonistic activity efficiency.

scholarly journals Soils from HNV agriculture systems as source of microorganisms with antifungal activity

2018 ◽  
Vol 2 (4) ◽  
pp. 196-199
Author(s):  
Matei Sorin ◽  
Matei Gabi-Mirela ◽  
Dumitrașcu Monica
Keyword(s):  
Antifungal Activity ◽  
Plant Pathogens ◽  
Culture Media ◽  
Trichoderma Viride ◽  
Culture Method ◽  
Sole Source ◽  
Fungal Species ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Agriculture Systems

Abstract Soils from rural zones with high natural value (HNV) agriculture systems are an important source of beneficial microbial species that can be useful for various biotechnological purposes, such as transfer of suppressiveness against plant pathogens from suppressive to disease-inducing soils by using inoculation with antagonistic selected strains. The main goal of the paper was to present the results of the research carried out on strains isolated from soil microbial populations in HNV agriculture system (Mureș county, Romania) responsible for specific suppressiveness against soil-borne phytopathogens. The dual culture method was used for assessing the mechanisms involved in antagonism against a plant pathogenic strain from genus Fusarium. The global microbial activity measured as soil respiration was intense. Total counts of bacteria and fungi estimated by dilution plate were also high. The community of heterotrophic aerobic bacteria included 13 species. Associations of fluorescent pseudomonads and actinomycetes were dominant and presented antagonistic activity against Fusarium. Twenty fungal species presented cellulolytic capability evidenced by growth on culture media with cellulose as sole source of carbon. Over cellulolytic capacity, the selected isolate of Trichoderma viride presented antagonistic activity against pathogenic Fusarium strain. Both biochemical mechanism and hyperparasitism were evidenced as involved in its antifungal activity.

scholarly journals Antagonistic screening of Trichoderma spp. isolated from patchouli rhizosphere

2022 ◽  
Vol 951 (1) ◽  
pp. 012021
Author(s):  
Muzakir ◽  
Hifnalisa ◽  
J. Jauharlina ◽  
Rina Sriwati
Keyword(s):  
Plant Pathology ◽  
Culture Method ◽  
Antagonistic Activity ◽  
Potato Dextrose Agar ◽  
Dual Culture ◽  
Fusarium Spp ◽  
Trichoderma Spp ◽  
Pogostemon Cablin

Abstract The objective of this research was to determine the antagonistic activity of Trichoderma spp. isolated from patchouli rhizosphere (Pogostemon cablin Benth.). Another objective was to perform antagonistic screening of these fungi to inhibit the growth of the wilted pathogen Fusarium spp. In vitro research was conducted in the Laboratory of Plant Pathology, Universitas Syiah Kuala, from January to June 2020. The study used a completely randomised design with five treatments and three replications. The antagonistic screening was carried out by using the dual culture method of Trichoderma spp. against Fusarium spp. with the medium of Potato Dextrose Agar (PDA). The result showed that five isolates of Trichoderma have different antagonistic percentages in inhibiting the Fusarium. The highest antagonistic activity was found from isolate 2 and the lowest value was shown by isolate 3.

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