scholarly journals Chemical Constituents and Antioxidant Activity of Melothria scabra Naudin Fruits

2021 ◽  
Vol 4 (4) ◽  
pp. 283-292
Author(s):  
Harni Sartika Kamaruddin ◽  
Megawati Megawati ◽  
Nurliana Nurliana ◽  
Carla Wulandari Sabandar
Keyword(s):  
Ethyl Acetate ◽  
Total Phenolics ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Scientific Information ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Total Flavonoids ◽  
Antioxidant Agents ◽  
Organic Fractions

The fruit of Melothria scabra Naudin is traditionally used by natives of South East Sulawesi and has economic values in the local markets. Nonetheless, little scientific information was gained from this plant to support its development for nutraceutical and pharmaceutical aspects. This study aimed to investigate the phytochemicals contained in the ethanol extract and organic fractions (methanol, ethyl acetate, and hexane) of the fruits using specific reagents and an LC-MS/MS analysis, as well as to evaluate their total phenolics, total flavonoids, and DPPH radical scavenging activity using a dot-blot staining and spectrophotometric assays. Results showed that the fruits of M. scabra contained alkaloids, tannins, flavonoids, terpenoids, and saponins. Six compounds were successfully identified from the ethanol extract of the fruits for the first time that is D-1-[(3-carboxypropyl)amino]-1-deoxyfructose (1), fructose-C3H5NO (2), valine (3), 1β, 3α, 9β-trihydroxyeudesma-5,11(13)-dien-12-oic acid (4), Cucurbitacin B-2-O-α-L-rhamnopyranosyl-β-D-glucopyranoside (5), and 2-heptyl-3-hydroxy-4(H)-quinolone (6). Total phenolics in the extract and organic fractions were in the range of 54.2 ± 2.4 to 259.1 ± 8.4 mg GAE/g, while total flavonoids were in the range of 1.6 ± 0.2 to 22.4 ± 0.2 mg QE/g. The ethanol extract and its organic fractions (methanol and ethyl acetate) were potent radical scavengers with SC50 values ranging from 20.7 to 37.5 µg/mL when compared with ascorbic acid, gallic acid, and quercetin (SC50 of 2.8 to 9.4 µg/mL). This study concludes that M. scabra fruits could be developed as a source of natural antioxidant agents for nutraceuticals and pharmaceuticals purposes.

Investigation of Compounds and Biological Activity of Selected Indonesian Marine Sponges

2020 ◽  
Vol 10 (3) ◽  
pp. 312-321
Author(s):  
Idin Sahidin ◽  
Carla W. Sabandar ◽  
Wahyuni ◽  
Rini Hamsidi ◽  
Sandra Aulia Mardikasari ◽  
...  
Keyword(s):  
Biological Activity ◽  
Antioxidant Activities ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Marine Sponges ◽  
Chemical Screening ◽  
Methanol Extracts ◽  
Ms Analysis ◽  
Antioxidant Agents

Background: Marine sponges provided a great source of natural products with promising biological activity. This study was aimed to investigate the chemical constituents of methanol extracts of selected Indonesian marine sponges (Callyspongia sp., Clathria sp., Melophlus sarasinorum, and Xestospongia sp.), collected from the Saponda Islands, Southeast Sulawesi, Indonesia as well as to evaluate their antimicrobial and antioxidant activities. Methods: LCMS/MS analysis used to identify the compounds. Agar well diffusion and DPPH assays were used to evaluate the antimicrobial and antioxidant activities. Results: Chemical screening reported alkaloids, terpenoids, steroids, and saponins from all investigated sponges. The LC-MS/MS analysis identified various compounds which mainly contained steroids. Antimicrobial activity (against Bacillus subtilis, Escherichia coli, Salmonella enterica, and Candida albicans) was only shown by the Xestospongia sp. extract. Meanwhile, extracts of M. sarasinorum, Xestospongia sp., and Callyspongia sp. exhibited potent radical scavenging activity. Conclusion: The study concluded that the selected sponges could provide various groups of compounds. Methanol extracts of these sponges could be used as sources of antimicrobial and antioxidant agents.

scholarly journals RELATIONSHIP BETWEEN ANTIOXIDANT PROPERTIES AND NUTRITIONAL COMPOSITION OF SOME GALACTOPOIETICS HERBS USED IN INDONESIA: A COMPARATIVE STUDY

2016 ◽  
Vol 8 (12) ◽  
pp. 236 ◽  
Author(s):  
Ade Chandra Iwansyah ◽  
Rizal M. Damanik ◽  
Lilik Kustiyah ◽  
Muhammad Hanafi
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Nutritional Composition ◽  
Total Flavonoids ◽  
Wide Range ◽  
Phenolics Content

<p><strong>Objective: </strong>The objectives of this research were to evaluate and compare the antioxidant activity; phenolics and flavonoids contents; and chemical composition of some galactopoietic herbs used in Indonesia and to find a possible relationship between antioxidant activity; phenolics, flavonoids and chemical composition of these plants.</p><p><strong>Methods: </strong>A wide range of analytical parameters was studied, including yield; pH; water activity (a<sub>w</sub>); total color difference; phenolics and flavonoids content; moisture; ash; protein; fat; carbohydrate; fiber; calcium; zinc; magnesium; potassium; iron content; and the data were analyzed by partial least squares (PLS) regression analysis and principal component analysis (PCA) to evaluate the correlation between the parameters and classification of the plants. Folin–cioceltaeu assay, aluminum chloride assay and DPPH scavenging assay were used to evaluate of total phenolics content and free radical scavenging activity of the ethanolic extract of galactopoietic herbs.</p><p><strong>Results: </strong>All of the galactagogue herbs, <em>viz</em>. <em>Plectranthus amboinicus </em>(Lour.) (PA), <em>Sauropus androgynus</em> (SA), <em>Moringa oleifera</em> (MO), and <em>Carica papaya</em> leaves (CP) exhibited good results of TPC, TFC and antioxidant activity. MO contained the highest average of total phenolics content (53.89 mg GAE/g). SA with a ratio of total flavonoids to total phenolics (TF/TP) which is 1.59 contained the highest average of total flavonoids and antioxidant activity (IC<sub>50</sub>), 43.63 mg QE/g and 126.13 μg/ml, respectively, followed by MO&gt;CA&gt;PA. Based on PLS and PCA analysis, antioxidant activity correlated with fat, protein, phenolics content, flavonoids content and pH, but only the total flavonoids content correlated strongly with antioxidant activity.<strong></strong></p><p><strong>Conclusion: </strong>This provides useful data about the studied relationships and chemical patterns in some galactopoietic herbs used and provides a helpful tool for understanding much more about the interpretation of results from the experiments and the mechanism of biosynthesis in plants.</p>

scholarly journals ANTIOXIDATIVE ACTIVITIES AND CHEMICAL CONSTITUENTS OF THE ETHYL ACETATE EXTRACT FROM JASMINUM UNDULATUM KER-GAWL

2011 ◽  
Vol 14 (2) ◽  
pp. 50-57
Author(s):  
Anh Thi Tran ◽  
Binh Thanh Nguyen ◽  
Hoai Thi Cam Ho ◽  
Huong Dang Thien Bui ◽  
Mai Thi Thanh Nguyen
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Antioxidative Activity ◽  
Chemical Constituents ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

From the total crude ethanol extract of Jasminum undulatum Ker Gawl.’s leaves and stems, five fractionss were obtained by partitioning with petroleum ether, chloroform, ethyl acetate and n-butanol solvents. These five fractions were investigated for antioxidative activity using the DPPH radical scavenging and nitric oxide-inhibitory assay. All the fractions showed antioxidative activity except the petroleum ether fraction. Among the fractionss, the ethyl acetate fraction was the most potent fraction in both assays with the SC50 values of 5.30 μg/ml and 80.90 μg/ml, respectively. Further investigation on the eight sub-fractions isolated and extracted from the ethyl acetate fraction showed that one of these sub-fractions, the TE6 sub-fraction, showed the most significant antioxidative activity with the SC50 values of 3.15 μg/ml and 61.83 μg/ml respectively in the DPPH radical scavenging and nitric oxide-inhibitory assay. From the TE4 and TE6 sub-fractions, three compounds were isolated, including p-tyrosol (1), protocatechuic acid (2) and hydroxytyrosol (3). The structure of those compounds were elucidated by spectrometric methods IR, MS, 1D-NMR, and 2D-NMR.

scholarly journals STUDY ON Hibiscus tiliaceus LEAVES AS ANTIBACTERIAL AND ANTIOXIDANT AGENTS

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Mohamad Syahmie Samsudin ◽  
Yosie Andriani ◽  
Purbowatiningrum Ria Sarjono ◽  
Desy Fitrya Syamsumir
Keyword(s):  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Inhibition Zone ◽  
Klebsiella Pneumonia ◽  
Scavenging Activity ◽  
Cancer Disease ◽  
Antioxidant Agents ◽  
Dpph Method ◽  
Positive Results

This study aimed to evaluate antioxidant and antibacterial activities of methanol, hexane, dichloromethane, ethyl acetate, and water crude sample fractions of H. tiliaceus leaves. Various type of samples play an important role to determining which compound that will give the positive results in antioxidant test (inhibiting free radical scavenging activity) which they probably have potency for preventing the cancer disease. Furthermore, those types of samples were also tested to the antibacterial test as their possibilities to be created as new antibiotics in the future. This research also focused on what compound groups that actually present in H. tiliaceus leaves by using the phytochemistry test. Antioxidant potency of H. tiliaceus leaves extracts for the radical scavenging activity for quantitative assay was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial potency was measured by the Muller Hinton Agar (MHA) by applied the sample chemical crude in the plate. The result showed that all chemical crude of H. tiliaceus leaves has high antioxidant property as methanol crude (MC) 308.416%; hexane crude (HC) 232.837%; dichloromethane crude (DC) 150.837%; ethyl acetate crude (EC) 73.623% and water crude (WC) 71.777% with respected to the readings of  100% from quercetin (Q) as a positive control. From antibacterial result, entire samples had shown the positive results towards both gram positive (Staphylococcus aureus and Bacillus subtilis) and gram negative bacteria (Klebsiella pneumonia and Escherichia coli). The ranges of the inhibition zone were both at 7-10 mm and 10-15 mm. The phytochemistry test determine that the samples actually contained a lot of compounds which were proteins, carbohydrates, phenols/tannins, flavonoid, terpenoids, glycosides and also steroid. It is clearly indicated that H. tiliaceus leaves have potential to be used against the antioxidant and also as new antibacterial agents

scholarly journals TYROSINASE INHIBITION, 2,2-DIPHENYL-1-PICRYLHYDRAZYL RADICAL SCAVENGING ACTIVITY, AND PHYTOCHEMICAL SCREENING OF FRACTIONS AND ETHANOL EXTRACT FROM LEAVES AND STEM BARK OF MATOA (POMETIA PINNATA)

2017 ◽  
Vol 10 (17) ◽  
pp. 85
Author(s):  
Rani Sauriasari ◽  
Nur Azizah ◽  
Katrin Basah
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Stem Bark ◽  
Scavenging Activity ◽  
Tyrosinase Inhibitor ◽  
Active Fraction

Objective: This study aims to investigate the potency of matoa as a tyrosinase inhibitor and antioxidant and also to identify the chemical compounds in the most active fraction and an ethanol extract from the leaves and stem bark of matoa.Methods: The extracts were tested for their tyrosinase inhibitory activity by evaluating the formation of L-dopachrome at 490 nm. Antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The most active extract from liquid-liquid partition analysis was fractionated, and then, the assays were performed.Results: The ethanol extract of leaves and stem bark of matoa showed weak anti-tyrosinase activity (percent inhibition was 24.54±0.22% and 21.93±0.57%, respectively, final concentration 200 μg/mL), but they showed strong DPPH radical scavenging activity (IC50 values were 6.11 μg/mL and 5.47 μg/mL, respectively). The ethyl acetate fraction was the most active fraction with an IC50 value of 5.38 μg/mL. Ethanol extract from the leaves and stem bark of matoa and the ethyl acetate fraction contains flavonoids, tannins, saponins, triterpenoids, and glycosides.Conclusion: Matoa does not have potency as a tyrosinase inhibitor, but it has good antioxidant activity, although still lower than that of quercetin.

scholarly journals Antioxidant Activity and Chemical Composition of Torreya grandis cv. Merrillii Seed

2009 ◽  
Vol 4 (11) ◽  
pp. 1934578X0900401
Author(s):  
Haiming Shi ◽  
Huandi Wang ◽  
Mengyue Wang ◽  
Xiaobo Li
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Heart Diseases ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Content ◽  
Ethanol Extract ◽  
Torreya Grandis

Torreya grandis cv. Merrillii is an endemic tree species in China, seeds of which are used as a popular snack, possessing beneficial effects on preventing angiosclerosis and coronary heart diseases. In this study, antioxidant activity and chemical constituents of T. grandis cv. Merrillii seed (TGMS) were investigated. The antioxidant activity of different fractions and the ethanol extract was evaluated using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity, hydroxyl radical scavenging activity and lipid peroxidation assays. The oil, CH2Cl2 and n-BuOH fractions, and ethanol extract of TGMS all showed antioxidant activities in these models, especially the DPPH one. By GC-MS analysis, twenty-seven constituents were identified from the oil fraction of TGMS. The total content of phenolic compounds in the CH2Cl2 and n-BuOH fractions and ethanol extract was also determined by the Folin-Ciocalteau method as 17.6, 21.6 and 12.9 μg/mg, respectively. In addition, analysis of the CH2Cl2 fraction yielded four phenolic compounds: 4-hydroxybenzaldehyde, 4-methoxy pyrocatechol, coniferyl aldehyde, 4-hydroxy cinnamaldehyde, and two steroids, β-sitosterol and daucosterol. These results provide scientific support for the empirical use of TGMS as a medicinal food for cardiovascular diseases.

Free Radical Scavenging Activity of Some Legumes Hulls Extract and Its Efficacy on Oil Oxidative Stability

2020 ◽  
Author(s):  
Alaa ElDein El-Beltagy ◽  
Salman Alharthi
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Protection Factor

Abstract Background Synthetic antioxidants have toxigenic effects, there is therefore growing interest in substituting them with natural antioxidants. Attention is being focused on extracting them from agricultural industry residuals to minimize costs. Legume seed hulls could be cheap sources of such natural antioxidants. Objective This study aims to unravel potential free radical scavenging activity, antioxidant activity, and total phenolic and flavonoid contents of some legumes’ hulls extracted by different solvents and evaluate their efficacy to enhance sunflower oil stability. Method Legume hulls extracted by different solvents were evaluated for their antioxidant activity coefficient (AAC), free radical scavenging activity [by 2,2-diphenyl-1- picrylhydrazyl (DPPH)], and phenolic and flavonoids contents. The protection factor and induction periods (rancimat test) of the highest activity extracts were evaluated. Results Sunflower seed hull ethyl acetate extract, lupine seed hull ethanol extract, and mung bean hull petroleum ether extract exhibited stronger DPPH scavenging activity, AAC, and protection factor values than other solvents. Ethyl acetate extracts of sunflower seed hulls showed an antioxidant and scavenging activity close (P&gt;0.05) to that detected for α-tocopherol. Conclusions Legume hulls may possess strong free radical scavenging and antioxidant activity. The analogous effect between sunflower hull extract and α-tocopherol make it a potential, cheaper substitute of α-tocopherol in food systems. Highlights The analogous effect between sunflower hull extract and α-tocopherol give it the potency to allow substitution at a concentration of 0.5% of sunflower hull ethyl acetate or 1% of lupine hull ethanol extract instead of 0.5% α-tocopherol to enhance induction periods and protection factors of sunflower oil.

Chemical Constituents and Biological Activities of Senecio aegyptius var. discoideus Boiss

2012 ◽  
Vol 67 (3-4) ◽  
pp. 144-150 ◽  
Author(s):  
Wafaa Hassan ◽  
Amal Gendy ◽  
Hanan Al-youssef ◽  
Assem El-Shazely
Keyword(s):  
Ethyl Acetate ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Alcoholic Extract ◽  
Total Extract ◽  
Chemical Structures

1A new eremophilane sesquiterpene, 1-β-hydroxy-8-oxoeremophila-7,9-dien-12-oic acid (), in addition to two known fl avonol glycosides, rutin (2) and quercetin-3-O-glucoside- 7-O-rutinoside (3), was isolated from the ethyl acetate fraction obtained from the aqueous alcoholic extract of the aerial parts of Senecio aegyptius var. discoideus Boiss. (family Asteraceae). The chemical structures of the isolated compounds were established by 1D and 2D NMR analysis (1H, 13C, COSY, HMQC, HMBC), MS and UV data, and through comparison with the literature. The ethyl acetate fraction and the isolated rutin showed significant cytotoxic activity against colorectal carcinoma (HCT 116) and to less extent against brain (U 251) and breast carcinoma (MCF 7). The ethyl acetate fraction showed a significant level of activity against Klebsiella pneumoniae, while the total extract showed the best antifungal activity against Candida albicans and Saccharomyces cerevisiae. DPPH radical scavenging activity of the ethyl acetate fraction was significant (96.7%) when compared to ascorbic acid. It also showed anti-inflammatory activity but no diuretic effect

scholarly journals EVALUATION OF ANTIOXIDANT AND ANTIFUNGAL PROPERTIES OF PALU SHALLOT (Allium ascalonicum L VAR. Aggregatum)

2021 ◽  
Vol 9 (Spl-2-ICOPMES_2020) ◽  
pp. S215-S221
Author(s):  
Muhammad Sulaiman Zubair ◽  
◽  
Agustinus Widodo ◽  
Mira Fatmasari ◽  
Febriana De’e ◽  
...  
Keyword(s):  
High Performance ◽  
Total Phenolics ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Flavonoids ◽  
Total Flavonoids Content ◽  
Quercetin Concentration

Shallot is one of the typical plants at Palu, Central Sulawesi, Indonesia, famous by local people as fried Shallot. It is used as a cooking spice and traditional medicine for treating various diseases. This study was carried out to assess the phytochemical constituent including total phenolics, total flavonoids, and quercetin content of the Palu shallot (Allium ascalonicum L var. aggregatum), and to determine the antifungal and antioxidant properties of this plant ethanolic extract. Total phenolics/ flavonoids and quercetin concentration were determined by spectrophotometry UV-Vis and Reverse Phase - High-Performance Liquid Chromatography (RP-HPLC) methods. Antifungal activity and antioxidant capacity of the ethanolic extract was assayed by using diffusion agar and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Results of the study revealed that the total flavonoids content of the ethanolic extract was 0.3634 ± 0.018 mg QE/100 mg while total phenolics content was 0.4834 ± 0.003 mg GAE/100 mg. Meanwhile, the quercetin content was 65.46±0.0002 mg/kg. Further, ethanolic extract of Palu shallot also showed the radical scavenging activity with IC50 of 0.1398 mg/mL and growth inhibition on Candida albicans with inhibitory zone diameter range from 7.57 to 16.51 mm. This study confirms the high quality of Palu shallot as it has high total flavonoids, represented by the high quercetin concentration, and it is proposed to be a source for an antioxidant and antifungal medicinal herb.

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