scholarly journals Potential Use of Lipid Waste Industrial Residues for Lipase, Phospholipase and Bio surfactant Production Using a Newly Isolated Bacillus Safensis Strain

2020 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Surface Tension ◽  
Soap Stock ◽  
Culture Medium ◽  
Lipolytic Activity ◽  
Oil Refining ◽  
Soap Industry ◽  
Bacillus Safensis ◽  
Surfactant Production ◽  
By Products ◽  
Potential Use

The aim of this work was to produce lipases by a newly bacterial strain using as substrate, reach lipid waste by products from oil refining or commercially soap industry. For the fermentation tests, two substrates were investigated to produce lipases: soap stock (solid lipid waste from an oil refining industry) and glycerin (liquid lipid waste from soap industry). The higher level lipases production was obtained with the soap stock as the sole carbon source. Different parameters such as pH, temperature of the medium and incubation time were optimized. A correlation was also obtained between detected lipolytic activity and reduction of surface tension in the culture medium. The surface tension decreased from 50 to 25.7 mN/m indicating that biosurfactants were produced in the culture medium. As soap stock contains phospholipids molecules, this by product also enhances phospholipases production by the newly Bacillus safensis strain.

scholarly journals Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

ScientiaTec ◽  
2020 ◽  
Vol 7 (03) ◽  
Author(s):  
Giandra Volpato ◽  
Victória Furtado Migliavacca ◽  
Bruna Coelho de Andrade ◽  
Júlio Xandro Heck ◽  
Marco Antônio Záchia Ayub
Keyword(s):  
Organic Synthesis ◽  
Culture Medium ◽  
Lipolytic Activity ◽  
Gum Arabic ◽  
Lipase Production ◽  
Culture Conditions ◽  
Search And Selection ◽  
Triton X ◽  
Potential Use ◽  
Selection Of

The industrial application of lipolytic enzymes has been studied mainly due to the ability of these enzymes in catalyze reactions of synthesis and their stability in various organic solvents. One possibility is the use of lipase the organic synthesis, taking advantage as the generation of waste and difficult recovery of sub bioproducts. In this work, we carried out a selection of eighty-four isolates of Bacillus amazonian for lipase production, of which 30 strains showed lipolytic activity. The study of the culture conditions was performed through a Plackett-Burman experimental design using the strain that presented the highest lipolytic activity in a culture medium using glycerol as substrate.  The studied conditions were: concentration of soybean oil, olive oil, triton X-100, gum arabic, glycerol, and (NH4)2SO4, pH, temperature and concentration of inoculums. The best result obtained were 27 U/L in 48 h of cultivation by Bacillus circulans BL53. This work shows that the search and selection of microorganism with lipolytic activities can facilitate the discovery of new lipases, with potential use as by-product surplus.

scholarly journals Characterization and optimization of extracellular enzymes production by Aspergillus niger strains isolated from date by-products

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Reda Bellaouchi ◽  
Houssam Abouloifa ◽  
Yahya Rokni ◽  
Amina Hasnaoui ◽  
Nabil Ghabbour ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Culture Medium ◽  
Extracellular Enzymes ◽  
Enzyme Expression ◽  
Specific Enzyme ◽  
Enzymatic Production ◽  
High Production Rate ◽  
High Production ◽  
Incubation Temperatures ◽  
By Products

Abstract Background This work aims to study the optimal conditions of the fermentation culture medium used for the production of extracellular enzymes (amylase, cellulase, lipase, and protease) from previously isolated Aspergillus niger strains in date by-products. Results The five most powerful isolates selected based on the zone of degradation formed on Petri plates by the substrate were subjected to the quantitative evaluation of their enzymatic production. All five strains showed almost similar API-ZYM profiles, with minor variations observed at the level of some specific enzyme expression. The production of cellulase and amylase was depending on pH and incubation temperatures. ASP2 strain demonstrated the high production rate of amylase (at pH 5 and 30 °C) and cellulase (at pH 6 and 30 °C) for 96 h of incubation. Conclusion The A. niger showed the ability to produce several extracellular enzymes and can be used in the valorization of different agroindustrial residues.

scholarly journals Composition and Nutritional Value of Acid Oils and Fatty Acid Distillates Used in Animal Feeding

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 196
Author(s):  
Elisa Varona ◽  
Alba Tres ◽  
Magdalena Rafecas ◽  
Stefania Vichi ◽  
Ana C. Barroeta ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Nutritional Value ◽  
Oil Refining ◽  
Analytical Control ◽  
Dietary Energy ◽  
Feed Ingredients ◽  
Animal Feeding ◽  
By Products

Acid oils (AO) and fatty acid distillates (FAD) are oil refining by-products rich in free fatty acids. The objective of this study is their characterization and the identification of their sources of variability so that they can be standardized to improve their use as feed ingredients. Samples (n=92) were collected from the Spanish market and the MIU value (sum of moisture, insoluble impurities, and unsaponifiable matter), lipid classes, fatty acid composition, and tocol content were analyzed. Their composition was highly variable even between batches from the same producer. As FAD originated from a distillation step, they showed higher free fatty acid amounts (82.5 vs 57.0 g/100 g, median values), whereas AO maintained higher proportions of moisture, polymers, tri-, di-, and monoacylglycerols. Overall, the MIU value was higher in AO (2.60–18.50 g/100 g in AO vs 0.63-10.44 g/100 g in FAD), with most of the contents of insoluble impurities being higher than those in the guidelines. Tocol and fatty acid composition were influenced by the crude oil’s botanical origin. The calculated dietary energy values were, in general, higher for AO and decreased when a MIU correction factor was applied. The analytical control and standardization of these by-products is of the outmost importance to revalorize them as feed ingredients.

scholarly journals Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

2013 ◽  
Vol 33 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Cintia Anabela Mazzucotelli ◽  
Alejandra Graciela Ponce ◽  
Catalina Elena Kotlar ◽  
María del Rosario Moreira
Keyword(s):  
Bacterial Strains ◽  
Isolation And Characterization ◽  
By Products ◽  
Potential Use

scholarly journals Co-valorization of agro-industry by-products: effect of citrus oil on the quality of soap derived from palm fatty acid distillate and spent bleaching clay

2019 ◽  
Vol 8 (3) ◽  
pp. 571-575
Author(s):  
Teerasak Punvichai ◽  
Daniel Pioch
Keyword(s):  
Fatty Acid ◽  
Oil Refining ◽  
Bleaching Clay ◽  
Microbial Count ◽  
Basic Solution ◽  
Soap Content ◽  
Palm Fatty Acid Distillate ◽  
Spent Bleaching Clay ◽  
By Products ◽  
Fatty Acid Distillate

This study deals with the co-valorization of spent bleaching clay (SBC) and palm fatty acid distillate (PFAD) –by-products of palm oil refining plants- through soap manufacture. Obtained SBC and PFAD samples show differing acidity and saponification values depending on fatty acids and acylglycerols content. Soaps are prepared using the stoichiometric amount of NaOH, under the varying proportion of water introduced through the basic solution. The mixing SBC and PFAD (ratio 1:3), the reaction completion (92.5%) is surprisingly higher than expected, indicating a synergistic effect on the course of the saponification reaction. The water is also a critical parameter, 30% w/w of added water allowing the highest yield. When testing for cleaning efficiency the products having the highest soap content, those from individual by-products give a low microbial count reduction after hand-washing (30-37%). But a much better score (74%) is obtained when using SBC:PFAD soap mixtures. This improvement could be due to abrasive and absorption effects of the clay, combined with the high soap content. The acceptability through a panel test is good for all soaps when formulated with citrus oil. The most active product corresponds to a SBC:PFAD ratio close to the production one in refining plants. Therefore these results provide an easy way for co-valorising these by-products, after further optimizing the saponification reaction in this complex triphasic medium (aqueous solution, oil, clay).

Potential Use of Plant‐Based By‐Products and Waste to Improve the Quality of Gluten‐Free Foods

2021 ◽  
Author(s):  
Graziana Difonzo ◽  
Giuditta Gennaro ◽  
Antonella Pasqualone ◽  
Francesco Caponio
Keyword(s):  
Gluten Free ◽  
By Products ◽  
Potential Use

scholarly journals Evaluation of the Use of a Coffee Industry By-Product in a Cereal-Based Extruded Food Product

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1008
Author(s):  
Elisa A. Beltrán-Medina ◽  
Guadalupe M. Guatemala-Morales ◽  
Eduardo Padilla-Camberos ◽  
Rosa I. Corona-González ◽  
Pedro M. Mondragón-Cortez ◽  
...  
Keyword(s):  
Food Industry ◽  
Food Product ◽  
Toxicity Tests ◽  
High Fiber ◽  
Fiber Protein ◽  
Roasting Process ◽  
Extruded Product ◽  
Acute Toxicity Tests ◽  
By Products ◽  
Potential Use

The evaluation of by-products to be added to food products is complex, as the residues must be analyzed to demonstrate their potential use as safe foods, as well as to propose the appropriate process and product for recycling. Since coffee is a very popular beverage worldwide, the coffee industry is responsible for generating large amounts of by-products, which include the coffee silverskin (CS), the only by-product of the roasting process. In this work, its characterization and food safety were evaluated by chemical composition assays, microbiological determinations, aflatoxin measurements and acute toxicity tests. The results showed that CS is safe for use in food, in addition to providing dietary fiber, protein and bioactive compounds. An extruded cereal-based ready-to-eat food product was developed through an extreme vertices mixture design, producing an extruded food product being a source of protein and with a high fiber content. Up to 15% of CS was incorporated in the extruded product. This work contributes to the establishment of routes for the valorization of CS; nevertheless, further research is necessary to demonstrate the sustainability of this food industry by-product.

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