scholarly journals THE EFFECT OF PHOTODYNAMIC THERAPY AND PLATELET-ENRICHED PLASMA ON THE HEALING OF SKIN RADIATION ULCERS INFECTED BY STAPHYLOCOCCUS AUREUS

2020 ◽  
Vol 25 ◽  
pp. 338-352
Author(s):  
M. Krasnoselsky ◽  
◽  
O. Pushkar ◽  
L. Simonova ◽  
M. Myroshnychenko ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Radiation Ulcer ◽  
Group 3 ◽  
Group 2 ◽  
Radiation Ulcers ◽  
Infected Skin ◽  
Group 1

Objective. To determine in experiment the quality of healing of skin radiation ulcers infected with Staphylococcus aureus (S. aureus) under the photodynamic therapy (PDT) administration and the use of platelet-rich plasma (PRP). Materials and methods. The experiment was performed on 95 male WAG rats of 6 months of age, which were divided into three groups. Group 1 included animals in whom a skin radiation ulcers in the thigh area was simulated, followed by application of a 0.2 ml suspension of reference strain of S. aureus to its surface on the 7th day after irradiation. Group 2 included animals with S. aureus-infected skin radiation ulcers, in whom the PET was administered a day after infection contamination. Group 3 included animals with S. aureus-infected skin radiation ulcers, in whom the PDT was administered a day after infection in the morning, and the PRP was manifold injected in periwound area in the afternoon. The skin with underlying soft tissues from the area of radiation damage were the material for morphological examination. The hematoxylin and eosin, picrofuxin according to van Gizon, Mallory staining were applied to micropreparations. A morphometric study was conducted. Results. In animals with skin radiation ulcers, in whom the PDT was administered upon infection with S. aureus (group 2), compared with animals with simulated infected skin radiation ulcers without treatment (group 1), The activation (i.e. accelerating) of the healing occurred for the period from the 14th to the 52nd day of experiment due to the active processes of wound cleansing from necrotized tissues, less pronounced inflammatory changes in the lesion, and active of appearance and maturation of granulation tissue, less pronounced hemodynamic, ischemic and alternative disorders in the dermis, hypodermis, muscle tissue surrounding the wound cavity, activation of proliferative processes in epithelial layer localized in the marginal parts of the wound. Formation of pathological (hypertrophic or keloid) scar of the skin was the result of healing of skin radiation ulcer infected with S. aureus. In animals with radiation ulcers infected with S. aureus, in the case of PDT and PRP (group 3) the regenerative process was directed not only at accelerating the rate of healing, but also on restoration of original structure of the lost parts of the skin compared with only PDT administration (group 2). Acceleration of the healing of the infected skin radiation ulcer in animals of groups 2 and 3 was due to similar processes. Conclusions. Photodynamic therapy activates and accelerates the healing process of skin radiation ulcers infected with S. aureus and leads to formation of a pathological scar (hypertrophic or keloid). Healing of the infected S. aureus radiation ulcers occurs more actively upon the photodynamic therapy administration in combination with multiple periwound injections of the platelet-enriched plasma, compared with only photodynamic therapy administration, and finishes with an organotypic regeneration and almost complete skin recovery. Key words: photodynamic therapy, platelet-rich plasma, radiation skin ulcer, Staphylococcus aureus, morphology.

scholarly journals Histopathological effect of platelet-rich plasma on cranial dura mater defects

2021 ◽  
Vol 38 (4) ◽  
pp. 525-528
Author(s):  
Kemal PAKSOY ◽  
Kerameddin AYDIN
Keyword(s):  
Dura Mater ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Female Rats ◽  
Wound Healing Process ◽  
Surface Areas ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

There are many new studies in the selection of materials used in dura material repair. Platelet has important function in hemostasis and coagulation. Also, activated platelets initiate the wound healing process. They provide regeneration of tissue with the appropriate type of tissue. In this experimental study, we used platelet-rich plasma (PRP) on dura mater defect due to these properties. Materials and method: Our study started after we got the approval of Ondokuz Mayıs University Animal Experiments Local Ethics Committee. Thirty Wistar Albino female rats were used. The rats were divided into three groups, each one includes ten. Results: No significant difference was observed in the thickness and surface areas of dura mater in group 1 and group 2. Significant increase was observed in the thickness and surface areas of dura mater in the PRP applied group (Group 3) compared to other groups. New bone areas and new vasculature were observed more frequently in the PRP rooms group (Group 3). Statistical analysis of the data was done by using SPSS 21.0 for Mac (IBM Corporation) statistical package program and based on 0.05 significance level. A significant difference was observed between Group 1 and Group 3 (p> 0.05), a significant difference was observed between both Group 1 and Group 3 and between Group 2 and Group 3 (p <0.05). Conclusion: Providing the regeneration of dura mater in defect situations will contribute to the protection of the barrier feature. In line with this idea, it is thought that PRP can be beneficial for us to reach this goal.

scholarly journals STUDY OF THE PLATELET-RICH PLASMA CLINICAL EFFICACY IN THE TREATMENT OF KNEE OSTEOARTHRITIS

2016 ◽  
Vol 7 (3) ◽  
pp. 54-60
Author(s):  
G D Lazishvili ◽  
К A Egiazaryan ◽  
A A Akhpashev ◽  
M A Danilov ◽  
M A Strakhov ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Knee Osteoarthritis ◽  
Clinical Efficacy ◽  
Detection Rate ◽  
Platelet Rich Plasma ◽  
Analog Scale ◽  
Womac Index ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The study of platelet-rich plasma efficacy in the treatment of patients with knee osteoarthritis (OA) was performed. 188 patients were included and divided into three groups. Group 1 - 82 patients with OA, where hyaluronic acid was used for treatment, Group 2 - 36 patients, and Platelet Rich Plasma (PRP) and hyaluronic acid were used, Group 3 - 70 patients, only PRP was used. It`s been found that the use of PRP for treatment is characterized by a pronounced clinical efficacy compared with use of hyaluronic acid, which manifests in reduced detection rate of edema, hyperthermia and hyperemia. The use of PRP leads to pain reduction in the knee joint and increase the functionality of patients, that confirmed by more pronounced declining of visual analog scale indicators, Leken index and the WOMAC index values increase. The presented results of study are consistent with authors who believe that the lack of side effects and complications when using PRP indicates the safety of its use in clinical practice.

scholarly journals Reduced Mortality of Staphylococcus aureus Bacteremia in a Retrospective Cohort Study of 2139 Patients: 2007–2015

2019 ◽  
Vol 70 (8) ◽  
pp. 1666-1674 ◽  
Author(s):  
Eloise D Austin ◽  
Sean S Sullivan ◽  
Nenad Macesic ◽  
Monica Mehta ◽  
Benjamin A Miko ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cohort Study ◽  
Medical Center ◽  
Academic Medical Center ◽  
Rapid Diagnostics ◽  
Staphylococcus Aureus Bacteremia ◽  
Group 3 ◽  
Group 2 ◽  
Mssa Infection ◽  
Group 1

Abstract Background Understanding the changing epidemiology of Staphylococcus aureus bacteremia, as well as the variables associated with poor outcomes, can yield insight into potential interventions. Methods This study was a retrospective, observational cohort study of adult patients at an academic medical center in New York City who had S. aureus bloodstream infections between 1 January 2007 and 31 December 2015. Participants were divided into 3 periods: group 1 (2007–2009), group 2 (2010–2012), and group 3 (2013–2015) for trend analysis. All clinical strains were genotyped (spa.). The main outcome was 30-day all-cause mortality. Results There were 1264 episodes of methicillin-susceptible S. aureus (MSSA) and 875 episodes of methicillin-resistant S. aureus (MRSA) bacteremia, with a rising proportion due to MSSA (55% group 1; 59% group 2; 63% group 3; P = .03.) There were no significant changes in average age, gender, Charlson score, and distribution of strain genotypes. Mortality in MRSA infection was unchanged (25% group 1; 25% group 2; 26% group 3), while mortality in MSSA infection significantly declined (18% group 1; 18% group 2; 13% group 3). The average time to antistaphylococcal therapy (AST) in MSSA infection declined during the study (3.7 days group 1; 3.5 group 2; 2.2 group 3). In multivariate analysis, AST within 7 days of initial positive MSSA culture was associated with survival. Conclusions Mortality in MSSA bloodstream infection is declining, associated with a decrease in time to targeted therapy. These results emphasize the potential for rapid diagnostics and early optimization of treatment to impact outcomes in MSSA bacteremia.

scholarly journals Clinical and Microbiological Outcomes of Topical Aloe Vera Gel vs. Photochemotherapy as an Adjunct to Non-surgical Periodontal Treatment in Periodontitis

2020 ◽  
Vol 07 (03) ◽  
pp. e100-e105
Author(s):  
Mohamed Farouk Elsadek ◽  
Badreldin Mohamed Ahmed ◽  
Rayan M. Eskandrani ◽  
Tasneem Sobhy Fahmy
Keyword(s):  
Photodynamic Therapy ◽  
Aloe Vera ◽  
Antimicrobial Photodynamic Therapy ◽  
Clinical Attachment Level ◽  
Probing Depth ◽  
Aloe Vera Gel ◽  
Group 3 ◽  
Group 2 ◽  
Attachment Level ◽  
Group 1

AbstractThe present clinical trial aimed to assess the effectiveness of antimicrobial photodynamic therapy versus Aloe vera gel as an adjunct to scaling and root planing on periodontal and microbial outcomes in patients with periodontitis. Eligible patients undergoing nonsurgical periodontal treatment were divided into 3 groups: group 1: antimicrobial photodynamic therapy; group 2: Aloe vera gel application; and group 3: scaling and root planing only. Clinical periodontal variables included the assessment of plaque scores, bleeding on probing, probing depth, and clinical attachment level gain. Plaque samples were collected to estimate microbial counts of Tannerella forsythia (T. forsythia) and Porphyromonas gingivalis (P. gingivalis). All measurements were recorded at baseline, 3 mo, and 6 mo. Statistical analysis of the given data was performed using the chi-squared test and ANOVA for clinical periodontal and microbiological data. Eighty-seven patients completed the trial. Bleeding on probing showed a significant reduction in group 2 compared with the other groups (p < 0.001). Group 1 showed a statistically significant reduction in probing depth and gain in clinical attachment level when compared to group 2 and group 3 (p < 0.05). Group 1 showed a statistically significant reduction in the counts of T. forsythia and P. gingivalis over a period of 3 mo (p < 0.05). The reduction was seen for T. forsythia only following 6 mo (p < 0.05). Group 2 showed a significant reduction for only T. forsythia at 3 mo (p < 0.05). Both antimicrobial photodynamic therapy and Aloe vera gel helped in reducing periodontal inflammation. Aloe vera gel showed additional benefit in reducing bleeding scores, while antimicrobial photodynamic therapy showed additional enhanced activity against periodontal pathogens and periodontal attachment level gain.

scholarly journals Osteocalcin expression of platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) added with hydroxyapatite (HA) in rabbit’s post extraction tooth sockets

2020 ◽  
Vol 32 (3) ◽  
pp. 243
Author(s):  
Meta Maulida Damayanti ◽  
Bethy Suryawathy Hernowo ◽  
Susi Susanah
Keyword(s):  
Bone Tissue ◽  
Program Analysis ◽  
Alveolar Bone ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Average Score ◽  
Platelet Rich Fibrin ◽  
Treatment Groups ◽  
Group 2 ◽  
Group 1

Introduction: Platelets play an important role in wound healing because it is a reservoir for growth factors and cytokines which is important in bone regeneration and soft tissue healing. The purpose of this study was to compare the use of scaffolds in platelet-rich fibrin (PRF) and platelet-rich plasma (PRP) which added with hydroxyapatite (HA) in the socket healing process after tooth extraction in the value of regenerating an alveolar bone tissue. Methods: The research was conducted at biomedical laboratory Bandung Islamic University. Eighteen rabbits (Oryctolagus cuniculus) with extracted anterior and inferior teeth were divided into 2 treatment groups and 3 observation times. The tooth socket is filled with PRF (Group 1) and PRP + HA (Group 2). The observation was conducted on Day 3, Day 7 and Day 14. Immunoexpression Osteocalcin was performed to assess the healing process of alveolar bone. Data was analyzed with the SPSS software program. Analysis of normality data by Shapiro-Wilk test, homogeneity of variance with Levene's test and comparison between treatment groups with the Chi-square test. Results: Group 1 shown the average score was higher than in Group 2 with a strong category of 72.2% for Group 1 and 56.6% for Group 2. Based on statistically,  there was no difference in osteocalcin immunoexpression between Group 1 and Group 2 with the p-value>0.05. Conclusion: Regeneration of rabbit’s alveolar bone tissue by application of PRF and PRP plus HA as scaffolds have results was similar. The use of PRF in post-extraction wound recovery is a better choice because it has an easy procedure and lower cost.

scholarly journals 1041. Staphylococcus aureus Bacteremia Bundle Adherence Pre- and Post-Implementation of Mandatory Infectious Diseases Consultation and Antimicrobial Stewardship Pharmacist Intervention

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S366-S367
Author(s):  
Kellie Arensman ◽  
Jennifer Dela-Pena ◽  
Jessica Miller ◽  
Erik LaChance ◽  
Maya Beganovic ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Infectious Diseases ◽  
Antimicrobial Stewardship ◽  
Source Control ◽  
Care Bundle ◽  
Review Group ◽  
Staphylococcus Aureus Bacteremia ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Abstract Background Infectious diseases consult (IDC) and antimicrobial stewardship (AMS) intervention independently demonstrate improved management of Staphylococcus aureus bacteremia (SAB). However, data supporting utilizing both strategies is limited. The objective of the current study is to assess evidence-based bundle adherence for SAB in the presence and absence of mandatory IDC and AMS pharmacist review in a multi-site health system. Methods This retrospective study included adult inpatients with SAB from January 2016 to December 2018 at seven hospitals. Outcomes were compared between three groups: pre-mandatory IDC and AMS review (group 1), post-mandatory IDC and pre-AMS review (group 2), and post-mandatory IDC and AMS review (group 3). The primary outcome was bundle adherence defined as: appropriate intravenous antimicrobial therapy, appropriate duration of therapy, 24–48-hour surveillance cultures until documented clearance, echocardiography, and removal of indwelling intravenous catheters, if applicable. Secondary endpoints included individual bundle components, source control, length of stay (LOS), 30-day bacteremia-related readmission, and in-hospital all-cause mortality. Results A total of 579 patients met the final inclusion criteria for analysis. Complete bundle adherence was achieved in 65% of patients for group 1 (n = 371), 54% for group 2 (n = 87), and 76% for group 3 (n = 121). Adherence to bundle elements was significantly higher in group 3 when compared with group 1 (odds ratio [OR] 0.58, 95% confidence interval [CI] 0.37–0.93), and group 2 (OR 0.37, 95% CI 0.20 – 0.67). No difference in bundle adherence was noted between groups 1 and 2. When comparing groups 1, 2 and 3, significant differences were seen in obtaining echocardiography (91% vs. 83% vs. 100%; P = 0.0378), and hospital LOS (10.5 vs. 8.85 vs. 12.0 days; P = 0.0149), respectively. Increased hospital LOS in group 3 may be due to nonsignificant higher rates of complicated bacteremia compared with groups 2 and 1 (32% vs. 44% vs. 43%, P = 0.09), respectively. No differences were noted for readmission or mortality. Conclusion The addition of AMS pharmacist review to mandatory IDC significantly improved quality care bundle adherence. Disclosures All authors: No reported disclosures.

scholarly journals Role of Biofilm-Associated Protein Bap in the Pathogenesis of Bovine Staphylococcus aureus

2004 ◽  
Vol 72 (4) ◽  
pp. 2177-2185 ◽  
Author(s):  
Carme Cucarella ◽  
M. Ángeles Tormo ◽  
Carles Úbeda ◽  
M. Pilar Trotonda ◽  
Marta Monzón ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Bovine Mastitis ◽  
Serum Samples ◽  
Group 3 ◽  
Group 2 ◽  
Vitro Analysis ◽  
Group 1

ABSTRACT Staphylococcus aureus is a common cause of intramammary infections, which frequently become chronic, associated with the ability of the bacteria to produce biofilm. Here, we report a relationship between the ability to produce chronic bovine mastitis and biofilm formation. We have classified bovine mastitis S. aureus isolates into three groups based on the presence of particular genetic elements required for biofilm formation: group 1 (ica + bap +), group 2 (ica +, bap negative), and group 3 (ica negative, bap negative). Overall, animals naturally infected with group 1 and 2 isolates had a lower milk somatic cell count than those infected with isolates of group 3. In addition, Bap-positive isolates were significantly more able to colonize and persist in the bovine mammary gland in vivo and were less susceptible to antibiotic treatments when forming biofilms in vitro. Analysis of the structural bap gene revealed the existence of alternate forms of expression of the Bap protein in S. aureus isolates obtained under field conditions throughout the animal's life. The presence of anti-Bap antibodies in serum samples taken from animals with confirmed S. aureus infections indicated the production of Bap during infection. Furthermore, disruption of the ica operon in a bap-positive strain had no effect on in vitro biofilm formation, a finding which strongly suggested that Bap could compensate for the deficiency of the PIA/PNAG product (a biofilm matrix polysaccharide). Altogether, these results demonstrate that, in the bovine intramammary gland, the presence of Bap may facilitate a biofilm formation connected with the persistence of S. aureus.

scholarly journals The Effects of Leukocyte- and Platelet-Rich Plasma (L-Prp) and Pure Platelet-Rich Plasma (P-Prp) an a Rat Endometriosis Model

2019 ◽  
Author(s):  
Ali Doğukan Anğın ◽  
İsmet Gün ◽  
Önder Sakin ◽  
Muzaffer Seyhan Çıkman ◽  
Zehra Meltem Pirioğlu ◽  
...  
Keyword(s):  
Platelet Rich Plasma ◽  
Saline Group ◽  
Group 4 ◽  
Vascular Congestion ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
The Relationship ◽  
Volume Difference ◽  
Group 1

AbstractOur aim was to investigate the effect of platelet-rich plasma (PRP) derivatives, which can be produced from the patient’s own blood and have minimal side effects, on endometriosis. To the best of our knowledge, this is the first study in the literature that studies the relationship between PRP and endometriosis. Endometriosis foci were created in the first operation. In the second operation (30th day) groups were formed. Group 1 (n= 8) was administered saline, group 2 (n= 7) leukocyte- and platelet-rich plasma (L-PRP), and group 3 (n= 8) pure platelet-rich plasma (P-PRP). Group 4 (n= 10) was used to obtain PRP. In the last operation (60th day), the endometriotic foci were measured, and then excised. There was no statistically significant difference between the pre and post volumes of the endometriotic foci, between their volume differences and volume difference rates (p > .05). However, it was observed that existing implant volumes in all groups decreased statistically significantly within their own groups by the end of the experiment compared to the previous volumes (p < .05). When the implants were assessed through histopathological scoring in terms of edema, vascular congestion, inflammatory cell infiltration, hemorrhage, epithelial line, and hemosiderin accumulation and immunohistochemical staining in terms of VEGF, there was no significant difference in the comparison between the groups. Although L-PRP and P-PRP generated more reduction in the endometriosis foci, they did not create any statistical differences.

scholarly journals What is better for rotator cuff tendinopathy: dextrose prolotherapy, platelet-rich plasma, or corticosteroid injections? A randomized controlled study

2020 ◽  
Vol 47 (1) ◽  
Author(s):  
Hala M. Abd Elsabour Sabaah ◽  
Mary A. Nassif
Keyword(s):  
Rotator Cuff ◽  
Platelet Rich Plasma ◽  
Tendon Healing ◽  
Controlled Study ◽  
Clinical Trial Registry ◽  
Western Ontario Rotator Cuff ◽  
Group 3 ◽  
Group 2 ◽  
Rotator Cuff Tendinopathy ◽  
Group 1

Abstract Background Rotator cuff tendinopathy (RCT) is a leading cause of shoulder pain and disability. Management is mainly conservative, but the limited ability of tendons to regenerate is the main cause of unsatisfactory results. So, we conducted our study to compare the efficacy of deep prolotherapy (glucose 25%), platelet-rich plasma (PRP), and betamethasone corticosteroid for treatment of RCT to find the most effective one based on clinical, functional, and radiological assessment. Results Regarding visual analog scale (VAS), it was significantly (p < 0.001) improved after injection among group 1 (prolotherapy group) and group 3 (steroid group) patients, while no significant improvement was noted among group 2 (PRP group) (p = 0.212) patients. The Western Ontario Rotator Cuff (WORC) Index significantly improved among the studied groups (p < 0.001, p = 0.049, and p < 0.001, respectively) after injection. Regarding the range of motion (ROM), a significant improvement (p = 0.029) was achieved in group 1 after injection but no significant improvements were noted among group 2 and 3 patients (p = 0.529 and 0.121, respectively). There was a significant improvement among group 1 and 2 patients (p < 0.001 and p = 0.020, respectively) regarding the grade of tendon lesions but no improvement occurred among group 3 patients (p = 0.470). Conclusion Prolotherapy injections improve shoulder ROM, VAS, WORC index, and rotator cuff tendon healing while PRP injections improve WORC index and tendon healing but steroid injection has no effect on healing. Trial registration PACTR202005610509496. Retrospective registration on May 25, 2020, Pan African Clinical Trial Registry.

PHOTODYNAMIC THERAPY IN COMPLEX TREATMENT OF PHLEGMON OF THE MAXILLOFACIAL AREA AND NECK

2021 ◽  
Vol 19 (4) ◽  
pp. 397-403
Author(s):  
L. A. Сherniak ◽  
Keyword(s):  
Photodynamic Therapy ◽  
Topical Treatment ◽  
Control Group ◽  
Present Moment ◽  
Complex Treatment ◽  
Traditional Treatment ◽  
Purulent Wound ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background. The treatment of phlegmon of the maxillofacial area and neck at the present moment remains difficult and insufficiently effective. Aim. To develop a method of using photodynamic therapy (PDT) for the treatment of phlegmon of the maxillofacial area and neck and to evaluate its effectiveness. Material and methods. A total of 92 patients with phlegmons of the maxillofacial area and neck were under observation. They were divided into 3 groups: group 1 – “control group 1” (30 patients) – where traditional treatment was used, group 2 – “control group 2” (30 patients) – in which low-intensity laser radiation was used in addition to topical treatment and group 3 – “experimental” (32 patients) – where PDT was used for topical treatment. Results. The data of clinical, cytological and bacteriological studies show that the local application of PDT has an anti-inflammatory effect and stimulates the reparative processes in the wound, and shortens the treatment period for patients. Conclusion. PDT improves the results of complex treatment of patients with phlegmons of the maxillofacial area and neck. Its application leads to the purification of a purulent wound on the 3rd (3;4) day, allows to apply secondary sutures on the 7th day and to shorten the period of inpatient treatment in comparison with the control groups from 3 to 9 days.

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