scholarly journals Larvicidal Activity of Bacillus sphaericus Strain SI-1 and Its Toxins against Culex quinquefasciantus Larvae

1970 ◽  
Vol 24 (2) ◽  
pp. 125-128
Author(s):  
MA Motalib Hossain ◽  
Alamgir Z Chowdhury ◽  
Abdullah Al-Mahin ◽  
Mustafizur Rahman ◽  
Khandakar Abdur Rahim

A local isolate of Bacillus sphaericus strain SI-1 had been investigated for its larvicidal activity against Culex quinquefasciantus mosquito. Wet spores of 72-h grown culture of the strain were sufficiently potent as mosquitocidal agents 3rd instar larvae of C. quinquefasciantus. After lyophilization the toxic potency of the strain increased sharply (165 times). The toxic crystal proteins had been solubilized in a hydroxyl-chloride buffer (pH 12.0) by three successive extractions. The majority of the proteins were found to be solubilized in water under these conditions. Increase of pH in the media by addition of buffer and its effect on the mortality of C. quinquefasciantus larvae had been checked. The extraction buffer was found to be non-lethal although significant rise in pH up to 10.44 was observed. The lethal concentration fifty (LC50) value of the crude protein was found to be 62.0 μg/ml. Keywords: Bacillus sphaericus SI-1, Biopesticide, Culex quinquefasciantus, Larvicidal activity, LyophilizationDOI: http://dx.doi.org/10.3329/bjm.v24i2.1256 Bangladesh J Microbiol, Volume 24, Number 2, December 2007, pp 125-128

2008 ◽  
Vol 38 (2) ◽  
pp. 255-262 ◽  
Author(s):  
Eleilza de Castro Litaiff ◽  
Wanderli Pedro Tadei ◽  
Jorge Ivan Rebelo Porto ◽  
Ila Maria de Aguiar Oliveira

Bioassays under laboratory conditions aiming to determine the larvicidal activity of Bacillus sphaericus were carried out on Anopheles darlingi and Culex quinquefasciatus. In order to estimate the toxicity through median lethal concentration (LC50) and the relative potency of the strains to B. sphaericus standard strain 2362, probit analysis was performed utilizing the POLO-PC program. The findings of LC50 pointed out high effectiveness on strains IB15 (0.040 ppm), IB19 and S1116 (0.048 ppm), IB16 (0.052 ppm) and S265 (0.057 ppm). Strain IB15 presented nearly 50% more potency than strain 2362 in bioassays conducted on A. darlingi. It was observed that IB16 and S1116 strains were the most powerful against C. quinquefasciatus, showing to be about 300-400% stronger than 2362 strain. The results show that laboratory conditioned evaluation can be an important way to select promising bacteria with entomopathogenic action on biolarvicides production for use on mosquitoes breeding sites.


2021 ◽  
Vol 8 (3) ◽  
pp. 202-211
Author(s):  
Ase Lestari ◽  
T.H Wahyuni ◽  
T.H Wahyuni ◽  
E. Mirwandhono ◽  
N. Ginting

Maggot black soldier fly (Hermetia illucens) is an insect that is environmentally friendly and can be mass produced and does not compete with humans. In addition, insect cultivation can reduce organic waste that has the potential to pollute the environment. Maggot black soldier fly (Hermetia illucens) has a high crude protein content of 42.1%. The length of the life cycle and the high and low nutrient content of the maggot are influenced by the maggot growing media used. In this study using EM4 as a bio-activator for fermentation of rice bran, coconut dregs, tofu dregs and palm kernel cake. The function of this fermentation is to increase the nutrients contained in this material which will later be used as a growing medium for maggot and maggot is one of the alternatives to feed protein sources from fish meal. The study was conducted experimentally using a completely randomized design (CRD) 5 treatments and 4 replications, with the weight of each experimental unit the media content is 1 kg and filled with 1 g of BSF eggs and the media size is 37 cm long, 26 cm wide and 10 cm high. The parameters of this study consisted of maggot production and maggot nutritional content, namely moisture content, ash content, crude protein, crude fat and carbohydrates. Based on research results P0 (100% fermented rice bran) obtained an average maggot production of 525g, KA maggot 8,16%, KAb maggot 7,83%, PK maggot 44,82%, LK maggot 21,62% and 17.58% maggot carbohydrates respectively. P1 (50% fermented rice bran + 50% fermented coconut dregs) obtained an average maggot production of 467.5g, KA maggot 9,57%, KAb maggot 9,03%, PK maggot 44,68%, LK maggot 20,51% and 16.20% maggot carbohydrates respectively. P2 (50% fermented rice bran + 50% fermented tofu dregs) obtained an average maggot production of 545.25g, KA maggot 9,02%, KAb maggot 8,32%, PK maggot 44,74%, LK maggot 18,57% and 20.09% maggot carbohydrates respectively. P3 (50% fermented rice bran + 50% palm kernel cake) obtained an average maggot production of 542g, KA maggot 9,16%, KAb maggot 9,22%, PK maggot 43,72%, LK maggot 19,87% and 18.02% maggot carbohydrates respectively. P4 (25% fermented rice bran + 25% fermented tofu dregs + 25% fermented coconut dregs + 25% palm kernel cake) obtained an average maggot production of 831.5g, KA maggot 9,08%, KAb maggot 9,35%, PK maggot 44,38%, LK maggot 17,01% and 20.18% maggot carbohydrates respectively. From the research results, it can be concluded that the use of culture media with a combination of 25% fermented rice bran + 25% fermented coconut dregs + 25% fermented tofu dregs + 25% fermented palm kernel meal is the best combination where the production reaches 831.5 g bsf and 20 carbohydrates. , 18% but could not increase moisture content, ash content, crude protein and crude fat.


1998 ◽  
Vol 93 (4) ◽  
pp. 441-444 ◽  
Author(s):  
Iléa Brandão Rodrigues ◽  
Waderli Pedro Tadei ◽  
José Manoel C Silva Dias

1976 ◽  
Vol 33 (5) ◽  
pp. 1208-1212 ◽  
Author(s):  
Alan V. Nebeker

Daphnia magna, the crayfish Pacifastacus leniusculus, and nymphs of the stoneflies, Acroneuria californica, A. pacifica, and Pteronarcys californica were tested in the laboratory to determine their survival in different concentrations of air-supersaturated water. The mean 96-h LC50 value (lethal concentration, 50% mortality) for D. magna was 122.5% total air saturation. The 7-day LC50 was 120% and the 10-day LC50 was 117.5%. The mean 96-h LC50 for crayfish was 147% total air saturation. The 7-day LC50 was 145% and the 10-day LC50 was 133%. The 10-day EC50 values (effective concentration, 50% immobilized) for stoneflies were 135% for A. californica and greater than 125% for A. pacifica and P. californica. Bubbles (emboli) were observed in body fluids and tissues, and general body distention occurred before death in Daphnia, crayfish, and stoneflies. Lethal threshold concentrations for Daphnia and crayfish were near 111 and 127%, respectively.


1983 ◽  
Vol 29 (6) ◽  
pp. 704-709 ◽  
Author(s):  
Jason A. N. Obeta ◽  
Nduka Okafor

Five media, formulated from dried cow blood, mineral salts, and seeds from four species of legumes, were assessed for growth, sporulation, and insecticidal properties of Bacillus sphaericus strain 1593. Bacterial powders, prepared from broth, were assayed against Culex quinquefasciatus, Anopheles gambiae, and Aedes aegypti. Good growth and sporulation were obtained with all the media. The highest number of viable cells and spores per millitre (8.6 × 108 and 8.1 × 108) were obtained in media containing ground seeds of Vignia unguiculata, Voandzeia subterranean, and Arachis hypogea. All powders were effective against C. quinquefasciatus and A. gambiae. Powders from media containing Arachis hypogea were the most effective with LC50's of 4.344 × 10−3 ± 1.650 × 10−4 and 0.193 ± 1.376 × 10−2 μg/mL for C. quinquefasciatus and A. gambiae, respectively. Aedes aegypti larvae were only slightly susceptible to the powders. This investigation shows that these media can be used for the production of B. sphaericus 1593 primary powder.


2019 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Rohendi Rohendi ◽  
Danar Dono ◽  
Andang Purnama

Barringtonia asiatica is one of the plants that the seeds extracts has insecticidal properties. However, the bioactivity of this seed extract has not been tested against Chilo sacchariphagus larvae which is the main pests in sugar cane. The treatment was carried out at several levels of concentration of methanolic seed extract of B. asiatica. The 15 cm of fresh sugarcane shoot dipping into the extract solution in some second. After the solution of extract on the sugarcane shoot dried, the first instar of larvae infested to the sugarcane shoot and then put into 20 cm test tube with 3 cm of diameter.  The duration of feeding on treated sugarcane shoot was two days and on the following day the larvae were fed on sugar cane shoots until the survived larvae reached the 5th instar. The results of the experiment indicated that B. asiatica seed extract was toxic to C. sacchariphagus with an LC50 value of 0.398% at 16 days after treatment. Besides toxic, B. asiatica seed extract also has atifeedant activity, and inhibits the growth of immature of C. sacchariphagus in all tested concentrations.Keyword: Toxicity, antifeedant, lethal concentration, Barringtonia asiatica, Chilo sachariphagus


2003 ◽  
Vol 69 (2) ◽  
pp. 1331-1334 ◽  
Author(s):  
Hyun-Woo Park ◽  
Dennis K. Bideshi ◽  
Brian A. Federici

ABSTRACT A novel recombinant Bacillus thuringiensis subsp. israelensis strain that produces the B. sphaericus binary toxin, Cyt1Aa, and Cry11Ba is described. The toxicity of this strain (50% lethal concentration [LC50] = 1.7 ng/ml) against fourth-instar Culex quinquefasciatus was higher than that of B. thuringiensis subsp. israelensis IPS-82 (LC50 = 7.9 ng/ml) or B. sphaericus 2362 (LC50 = 12.6 ng/ml).


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