Comparative Study on Production of Antibody in Broilers Vaccinated with Four Lyophilized Newcastle Disease Vaccine

1970 ◽  
Vol 27 (1) ◽  
pp. 1-5
Author(s):  
S Kamrunnahar ◽  
MM Alim ◽  
AHM Taslima ◽  
MA Islam ◽  
NC Paul

A comparative efficacy of four lyophilized Newcastle disease vaccine, namely, Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® in regard to the production of Himagglutination Inhibition (HI)- antibody was accomplished. For this, a total of 75 chicks were equally divided into five experimental groups such as, A, B, C, D and E. The HI antibody titers of group A vaccinated on 5 and 21 days of age with Medivac NDLaSota ® fluctuated among the Mean ± SD of 89.60 ± 33.05, 102.40 ± 33.05 and 192 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. As regards, group B administered with BCRDV® on the same days, the HI titers varied with the Mean ± SD of 83.20 ± 30.91, 102.40 ± 33.04 and 204.80 ± 66.09 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. When considered the HI antibody titers of group C inoculated with Izovac B1 Hitchner® on days 5 and 21 of age, HI titer exhibited the Mean ± SD of 80.00 ± 43.33, 96.00 ± 33.73 and 192.00 ± 67.46 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. Elucidation of HI antibody titers of group D birds receiving Cevac Vitapest-L® on same days were recorded to be of Mean ± SD of 96.00 ± 33.73, 115.20 ± 26.98 and 320.00 ± 173.31 at 15 (10 DPV), 19 (14 DPV) and 31 (26 DPV) days of age respectively. In birds of unvaccinated control group E, HI titers were found to be ranged from 32- 64, 16-32, 8-16, 4-8 and 2-4 with Mean ± SD of 48.00 ± 16.87, 24.00 ± 8.43, 11.20 ± 4.13, 5.20 ± 1.93 and 3.40 ± 0.97 on 3, 15, 17, 19 and 31 days of age respectively. Thus, it was found that birds of group A, B, C and D vaccinated with Medivac ND-LaSota®, BCRDV®, Izovac B1 Hitchner® and Cevac Vitapest-L® induced slightly higher level of HI antibody titers than that of BCRDV. Maternally derived antibody (MDA) persisted to a minimal level until the age of chicks of day 17 and later on MDA declined. Keywords: Vaccine; Antibody Titer; Newcastle Disease VirusDOI: http://dx.doi.org/10.3329/bjm.v27i1.9159 BJM 2010; 27(1): 1-5

2020 ◽  
Author(s):  
Obeagu Emmanuel Ifeanyi

The hematological features of Gongronema latifolium, aqueous leaves extract was evaluated using standard methods. After 10 days of consecutive infusions into 9 experimental animals (rabbits). The rabbits were monitored and the following parameters determined; hemoglobin (HB), PCV, Platelet, WBC, Differential White Blood Cell. The Rabbits were grouped into 4, one consisting of control (group A), group B was fed with 0.5 mg/kg, group C with 1.0 mg/kg, and Group D with 1.5mg/kg of the aqueous extract of Gongronemalatifolium. The mean values obtained for hemoglobin estimation for the control group is 5.9 ± 4.1 g/dl, 9.1 ± 2.9 g/dl for group B 10.2 ± 1.8 g/dl for group C and 12.8 ± 0.1 g/dl for group D with no significant increase on the PCV estimation, the mean value for the control (group A) is 17.7 ± 12.3%, 27.3 ± 8.7% for group B, group C (30.6 ± 5.4%) and D (28.4 ± 0.3) show increase that statistically significant (p > 0.01). the platelet counts of group C (600 ± 0 x 109/L) and D(600 ± 0 x 109/L) show significant increase (p > 0.01) when compared with the control (600 ± 00). But the platelet value of group B (550 ± 50 x 109/L) shows no difference. No significant changes were observed in the White Blood Counts of the test groups B (3.5 ± 0.5 x 109/L), C (1.9 + 2.1 x 109/L) and D(3.6 ± 0.4) when compared with the control group (2.9 ± 1.9). The values obtained from the differential White Blood Counts (Neutrophils, Lymphocytes, Eosinophils and Monocytes) were not significant. Therefore, Gongronemalatifolium, when properly taken as a nutritional diet, causes beneficial changes on hemoglobin, packed cell volumes and platelet counts of consumers.


2013 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Sreebas Chandra Sarkar ◽  
Sukumar Saha ◽  
Md Mansurul Amin ◽  
Md Golzar Hossain

The study was conducted to investigate the efficacy of Baby chick Ranikhet Disease Vaccine (BCRDV) and Ranikhet Disease Vaccine (RDV) produced by the Livestock Research Institute (LRI), Mohakhali, Dhaka. For this experiment, 100 day-old-chick was purchased from Phinex Hatchery Ltd., Gazipur. The chicks (n=100) were divided into two groups. In group A (n=50), vaccination was performed twice with BCRDV at 2 and 21 days of age through intraocular route (i/o) followed by once with RDV at 60-day of age through intramuscular (i/m) route. Group B (n=50) was kept as unvaccinated control. The immunogenicity of the vaccine was evaluated by measuring the serum HI antibody titers at 1-, 20-, 36-, and 76-day of age, while the vaccine efficacy was examined by a challenge infection experiment with a velogenic field isolate of NDV as well as passive protection test. It was observed that the maternal antibody titers of the unvaccinated control group B gradually declined from day 1 to day 76 of age. Conversely, after primary and secondary vaccination with BCRDV, the levels of serum HI titer slightly increased in vaccinated group A compared with those in control group B. Finally administration of RDV resulted in a sharp increase in HI titer, leading to protection from challenge infection with virulent field virus as well as passive protection test. These results clearly demonstrated that a prime-booster immunization with BCRDV and RDV, both produced by LRI, is effective to protect chicken against Newcastle disease (ND).DOI: http://dx.doi.org/10.3329/mh.v1i1.13706 Microbes and Health Vol.1(1) June 2012 pp.9-13


Author(s):  
MS Mahmud ◽  
MT Hossain ◽  
P Monoura ◽  
MM Amin

A study was conducted for the detection of persistence of maternal antibody as well as the comparative efficacy of Avinew (VG/GA Strain) and BCRDV (F Strain) vaccines against Newcastle disease during the period from January to April 2006 using 100 day-old broiler chicks divided into three groups namely A, B and C. Birds of groups A and B consisting each of 40 were primarily vaccinated intraocularly (IO) with Avinew and BCRDV respectively at the age of day three (3) and secondarily with the same vaccines, as the cases were, on day 20. Sera samples were obtained from 10 randomly selected birds on each occasion of day 1, 10, 17, 27, 30, 34 and 44 of age of birds. Birds of group C were maintained as unvaccinated control for the determination of existence of maternal antibody parallel to the day of vaccination and challenge. It was observed that following primary vaccination in case of group A, the HI titres with a mean ± SD on day 10 and 17 were 121.6 ± 19.2 and 60.8 ± 9.60 respectively as against at mean titres ± SD of 57.6 ± 12.8 and 30.4 ± 4.80 in case group B respectively. On the other hand, subsequent to secondary vaccination, sera samples obtained from group A on day 27 had a mean ± SD of HI titres 128 ± 0 and in case of group B, the performance of Avinew in respect of such titre was 57.6 ± 12.8 on day 27. Thus it was indicated that performance of Aninew in respect of elucidation of HI antibody was comparatively better than that of BCRDV. In case of unvaccinated control birds of group C, the mean of existence of maternal antibody with ± SD were 512 ± 0, 54.4 ± 14.66, 24 ± 8, 12.8 ± 8.54, ≤ 4, ≤ 4 and ≤ 4 at the age of day 1, 10, 17, 27, 30, 34 and 44 respectively. One half of vaccinated birds of groups A and B were subjected to challenge test with a virulent isolate of NDV on each of two occasion of day 30 and 44 of age of birds where it was observed that 100% of both the groups of A and B were refractory to each test whereas 95% and 85% of the remaining half of birds of groups A and B resisted the challenge exposure. It was found that maternal antibody against NDV in chicks persisted to a minimal until the age of day 27 and none at day 30 or 34. The analysis of HI titres by Student's t-test revealed that Avinew vaccinated group maintained significantly higher HI titres following primary and secondary vaccination as well as during first challenge than that of BCRDV vaccinated group. Key words: Avinew, BCRDV, efficacy, vaccine, broiler chicks   DOI = 10.3329/bjvm.v5i1.1304 Bangl. J. Vet. Med. (2007). 5 (1 & 2): 19-23


2015 ◽  
Vol 2 (1) ◽  
pp. 117-124 ◽  
Author(s):  
Bayzer Rahman ◽  
Tahmina Begum ◽  
Yousuf Ali Sarker ◽  
Md Quamrul Hassan ◽  
Sukumar Saha ◽  
...  

The present study is undertaken to compare the efficacy of alcoholic extracts of Black Peppers (Piper nigrum ) and Chutra leaves (Urtica dioica ) with a patent drug Esb3 against coccidiosis in chicken. 16 Fayoumi breed chickens were collected from a local farm and divided into four groups; A, B, C and D, each consisting of four chickens, Group A (control), Group B (alcoholic extracts of Black Peppers @ 9ml/kg bd wt.), Group C (alcoholic extracts of Chutra leaves @ 9ml/kg bd wt.) and Group D ( Esb3 @ 1 ml/ liter drinking water). All the treated chickens were kept under close observation for 18 days and data was collected at 3 days interval. In group B, two chickens died within 4-7 days of treatment and in group C one chicken died on 5th day of medication. All the chickens of control group died within 5-7 days of medication. Oocyst was counted for per gram of feces in all groups. Biochemical parameters like SGPT and haematological parameters like Total erythrocyte count (TEC), Haemoglobin count (Hb), Packed cell volume (PCV), Erythrocyte sedimentation rate (ESR) were determined. There were significant decrease in oocyst count in group B and C in compared to control and very few oocysts were present in faeces of 6 days onward of medication. All the chickens were survived in group D and oocyst started to disappear in the faeces from 3rd day onward of medication. Our study suggests Both Black Peppers and Chutra are effective against coccidiosis in chicken and Chutra is more effective than Black peppers.Res. Agric., Livest. Fish.2(1): 117-124, April 2015


1970 ◽  
Vol 7 (1) ◽  
pp. 275-280 ◽  
Author(s):  
M. M. Rahman ◽  
M. Mostofa ◽  
V. G. Barman ◽  
R. Mian ◽  
S.A. Mamun

The experiment was conducted to investigate the comparative efficacy of indigenous medicinal plants, dadmardan (Cassia alata) and neem (Azadiracta indica) and ivermectin against skin lesions in calves and goats. Twelve calves of 8- 12 months and twelve goats of 1-2 years of age irrespective of sex having skin lesions (ringworm, scabies, humpsore, wounds) were used in the experiment. Selected calves and goats were divided into four equals groups (group A,BC and D), each group consists of 3 calves and 3goats. The group A kept as control group. The group B was treated with ivermectin (Cevamec 1%(R) @200 ug/kg body weight subcutaneously ,group C with dadmardan ointment (30%) ointment topically  and group D with 30% combined ointment(dadmardan and neem leaves) topically. Calves and goats treated with ivermectin were almost cured within18 days and the rate of healing was 98.36% & 99.03% respectively whereas animals treated with dadmardan ointment cured at 30thdays of treatment  in calves and at 27thdays of treatment in goats and  the rate of healing was 99.80% & 99.88%  respectively and those treated with  combined ointments of dadmardan and neem leaves cured at 27thdays of treatment  in calves and at 24th day of treatment in goats  and the rate of healing was 99.94% & 99.35% respectively. Ivermectin treatment was more effective in comparison to other treatments considering the time required for healing. On the other hand, treatment with the onitmemts of indigenous medicinals plants were more effective considering the rate of healing. It may be concluded that the ointments of indigenous medicinals plants (dadmardan ointment and the combined ointment of dadmardan and neem leaves) may be used as alternative drugs for the treatment of skin lesions in calves and goats.


2012 ◽  
Vol 2 (1) ◽  
pp. 21-25
Author(s):  
MA Rahman ◽  
M Jahan ◽  
S Karmaker ◽  
MK Islam

The effects of some selected fatty acids supplement on hematobiochemical parameters were studied in rat. A total of twenty rats were randomly assigned into four groups. Group A was considered as control and fed with rat pellet. Group B was treated with 20 mg Soybean oil / kg feed, group C with 20 mg Mustard oil/ kg feed and group D with 20 mg Ghee /kg feed. The mean body weight of group B, C and D of 2nd and 3rd observation (30 and 60 days, respectively) was significantly (P> 0.01) higher compared to group A (Control). The highest body weight was observed in 3rd observation and it was 185.00 ± 2.76 g in group D and the lowest was in control group. TLC value of group D increased significantly (P<0.01) compared to control and was also found higher for group of B and C than that of control. Similar results were found for PCV value. DLC value was significant for none of the groups. The serum cholesterol level of group D increased significantly (P<0.01) but it was also found higher for the groups B and C than that of control. Similar results were found for blood glucose level and Serum Glutamic Pyruvic Transaminase (SGPT) where the values of group D increased significantly (P<0.01) compared to control and the values were also found higher for the groups B and C than that of control. DOI: http://dx.doi.org/10.3329/ijns.v2i1.10879 International Journal of Natural Sciences (2012), 2(1): 21-25


2015 ◽  
Vol 35 (4) ◽  
pp. 398-403 ◽  
Author(s):  
Y Alcaraz-Contreras ◽  
RP Mendoza-Lozano ◽  
ER Martínez-Alcaraz ◽  
M Martínez-Alfaro ◽  
MA Gallegos-Corona ◽  
...  

We studied the effect of silymarin and dimercaptosuccinic acid (DMSA), a chelating agent that was administered individually or in combination against lead (Pb) toxicity in rats. Wistar rats (200 ± 20) were randomly divided into five groups. Group A served as a control. Groups B–E were exposed to 2000 ppm of lead acetate in drinking water for 8 weeks. Group B served as a positive control. Group C received silymarin (100 mg kg−1 orally) for 8 weeks. Group D received DMSA (75 mg kg−1 orally) once daily for the last 5 days of treatment. Group E received DMSA and silymarin as groups C and D, respectively. The effect of Pb was evaluated and accordingly the treatments on blood lead levels (BLLs), renal system, and genotoxic effects were calculated using comet assay. The BLLs were significantly increased following the exposition of lead acetate. The administration of silymarin and DMSA provided reduction in BLLs. Silymarin and DMSA provided significant protection on the genotoxic effect of Pb. The toxic effect of Pb on kidneys was also studied. Our data suggest that silymarin and DMSA improve the renal histopathological lesions.


2021 ◽  
Vol 10 (2) ◽  
pp. 024-030
Author(s):  
Godwin Chinedu Uloneme ◽  
Demian Nnabuihe Ezejindu ◽  
Darlington Cyprian Akukwu ◽  
Amadi Chibundu Chiekezie

Background: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).ound: The extract of Corchorus olitorius has a reasonable content of vitamins A and C, calcium, iron and fibre, and therefore enjoys a universal application in the treatment of some disease conditions, even as the whole leaf is a very important component of food in so many cultures. Purpose: The study was designed to investigate the effect of Corchorus olitorius extracts on the testis of adult Wistar rats. Method: A total number of thirty two adult Wistar rats weighing between 180 and 200 grammes separated into four groups labeled A,B,C and D respectively were used for the study. Animals in group A which served as the control group were fed with the normal rat chow and water only. The group B rats were administered 100mg/kg body weight of aqueous extract of Corchorus olitorus; while those in group C were administered 500mg/kg body weight of the extract. The group D rats received 1000mg/kg body weight of the extract. For a period of four weeks, the different experimental animal groups received the respective aforementioned treatments once daily, around nine- o’clock in the morning through oral intubation. At the end of the 28 day treatment, the animals were sacrificed and the testes harvested for histological, investigation, and through cardiac puncture, blood samples for some hormonal studies was also collected and investigated using standard laboratory standards. Results: Observations made showed that the extract produced no histological distortions, degenerative or defective effects on the testicular tissues. The testosterone levels of group B, C, and D rats were observed to be significantly higher (P<0.005) than that of the group A (control group).


2021 ◽  
Vol 14 (10) ◽  
pp. 1508-1517
Author(s):  
Shan-Na Chen ◽  
◽  
Ying-Xue Ma ◽  
Song Chen ◽  
Guang-Hui He ◽  
...  

AIM: To investigate the protective effect of human umbilical cord mesenchymal stem cells (hUCMSCs) modified by the LIF gene on the retinal function of diabetic model rats and preliminarily explore the possible mechanism. METHODS: A stably transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor (LIF) was constructed. Overexpression was verified by fluorescent quantitative polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley rats were randomly divided into a normal control group (group A), streptozotocin-induced diabetic control group (group B), diabetic rats at 3mo injected with empty vector-transfected hUCMSCs (group C) or injected with LIF-hUCMSCs (group D). Four weeks after the intravitreal injection, analyses in all groups included retinal function using flash electroretinogram (F-ERG), retinal blood vessel examination of retinal flat mounts perfused with fluorescein isothiocyanate-dextran (FITC-dextran), and retinal structure examination of sections using hematoxylin and eosin staining. Expression levels of adiponectin (APN), high-sensitivity C-reactive protein (hs-CRP), and neurotrophin-4 (NT-4) in each group was detected using immunohistochemistry, PCR, Western blotting, and ELISA, respectively. RESULTS: A stable transgenic cell line of LIF-hUCMSCs was constructed. F-ERG and FITC-dextran examinations revealed no abnormalities of retinal structure and function in group A, severe damage of the retinal blood vessels and function in group B, and improved retinal structure and function in group C and especially group D. qPCR, ELISA, and Western blot analyses revealed progressively higher APN and NT-4 expression levels in groups B, C, and D than in group A. hs-CRP expression was significantly higher in group B than in groups A, C, and D, and was significantly higher in group C than in group D (P<0.05). CONCLUSION: LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 expression and downregulating hs-CRP expression in the retina.


2013 ◽  
Vol 21 (2) ◽  
pp. 84-89 ◽  
Author(s):  
Md Manjurul Karim ◽  
Md Abdul Wahab ◽  
Lubna Khondoker ◽  
Md Shirajul Islam Khan

A case-control study conducted in the Department of Dermatology and Venereology of Bangabandhu Sheikh Mujib Medical University, Dhaka from July 2008- December 2010. A total 60 female patients of telogen effluvium attending in outpatient department (OPD) were enrolled in the study with 30 having hair loss considered as case (Group-A) and another 30 females had no history of hair loss were considered as control (Group-B). The study included 60 patients and the mean age were 25.4±7 years ranging from 18 to 42 years in group A and 24.8±5.6 years ranging from 17 to 36 years in group B. Maximum number was found in the age group of 21- 30 years in both groups and nearly three fourth (73.4%) patients were unmarried in group A and 18(60.0%) in group B. Most of the patients were student in both groups, which were 21(70.0%) and 17(56.7%) in group A and group B respectively and majority of the the patients were HSC level in both groups, 15(50.0%) and 14(46.7%) in Group A and Group B respectively. Most of the patients came from middle class, which were 20(66.7%) and 16(53.3%) in group A and group B respectively. The mean serum ferritin were 18.8±8.1 g/L ranging from 4.5 to 36.54 g/L and 36.6±9.9 mg/L ranging from 18.46 to 56.3 mg/ L in group A and group B respectively. The mean Hb level was 11.5±1.4 gm/dl ranging from 8.5 to 14.0 gm/dl in group A and 12.8±1.1 gm/dl ranging from 10.5 to 14.5 gm/dl in group B. The mean difference of Hb level was statistically significant (p<0.05) between two groups in unpaired t-test. It can be concluded that low iron level is associated with telogen effluvium in women. DOI: http://dx.doi.org/10.3329/bjmed.v21i2.13617 Bangladesh J Medicine 2010; 21: 84-89


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