scholarly journals Production and Partial Characterization of Cellulase from Pseudomonas Isolates Obtained from Cow Dung and Municipal Solid Wastes

2016 ◽  
Vol 30 (1-2) ◽  
pp. 11-16
Author(s):  
Md Mehadi Hasan Sohag ◽  
Md Mahbub Hasan ◽  
Jahed Ahmed ◽  
Syed Nur Ahmed Daud ◽  
Md Khurshid Alam ◽  
...  
Keyword(s):  
Cellulase Activity ◽  
Basal Medium ◽  
Cellulase Production ◽  
Solid Wastes ◽  
Cow Dung ◽  
Optimum Level ◽  
Bacterial Isolates ◽  
Municipal Solid Wastes ◽  
Enrichment Technique ◽  
Transparent Zone

Cellulase producing bacteria were isolated from cow dung and municipal solid wastes using enrichment technique. Cellulase activity was determined on carboxymethyl cellulose (CMC) agar medium supplemented with 1% CMC. Production of clear zones by the bacterial isolates on CMC agar was considered as indicative of extracellular cellulase activity. The greater size of transparent zone diameter was found proportional to the higher amount of cellulase production. Two bacterial isolates producing significant clear zone were identified as Pseudomonas spp based on morphological, cultural and biochemical characteristics. Fermentation was carried out under shake flask conditions for production of cellulase from Pseudomonas isolates in a basal medium containing CMC, KH2PO4, K2HPO4, MgSO4, NH4SO4, CaCl2 and FeSO4 at pH 7.0. The assay of cellulase viz. endoglucanase and exoglucanase in terms of CMCase and FPase, respectively was done by measuring the release of the reducing sugar. Some parameters influencing the production of cellulase by these bacterial isolates were investigated. Optimum level of cellulase was produced after 48 h of fermentation at 37°C with 5% inoculums size under continuous agitation at 120 rpm in the growth medium of pH 7.0. Optimum temperature and pH for the activity of cellulase from these bacterial isolates was 40°C and 7.0, respectively. Cellulase from these isolates was found almost stable at 25-40°C and pH 6.0-8.0 for 1 h. Furthermore, the cellulase activity was stimulated by Ca2+ and K+ but inhibited by Hg2+ and Zn2+, whereas, Mg2+ showed very low effect. Results reported herein indicated that the Pseudomonas isolates can be used as producer of extracellular cellulase.Bangladesh J Microbiol, Volume 30, Number 1-2,June-Dec 2013, pp 11-16

scholarly journals Vermicomposting of Vegetable Wastes Using Cow Dung

2008 ◽  
Vol 5 (4) ◽  
pp. 810-813 ◽  
Author(s):  
K. Muthukumaravel ◽  
A. Amsath ◽  
M. Sukumaran
Keyword(s):  
Organic Matter ◽  
Solid Wastes ◽  
Cow Dung ◽  
Toxic Substances ◽  
Vegetable Waste ◽  
Municipal Solid Wastes ◽  
Vegetable Wastes ◽  
Land Filling ◽  
Plastic Trays

Municipal solid wastes are mainly from domestic and commercial areas containing recyclable toxic substances, compostable organic matter and others. With rapid increase in population, the generation of municipal solid wastes has increased several folds during last few years. Disposal of solid wastes can be done by methods like land filling, incineration, recycling, conversion into biogas, disposal into sea and composting. Vermicomposting is one of the recycling technologies which will improve the quality of the products. The present study aims to find out the possibility of utilization of vegetable wastes for vermiculture. EarthwormMegascolex mauritiicultured in plastic trays (45 x 30 x 30 cm) containing soil alone (control) (T1), soil + cow dung (T2), soil + vegetable waste (T3) and soil + vegetable waste + cow dung (T4) for 60 days. Nutrient values were determined from the compost and compared with that of the control. From these results, it was found that NPK values were maximum in compost obtained from vegetable waste with the use of cow dung.

CLEAN UTILIZATION OF MUNICIPAL SOLID WASTES AND ALTERNATIVE FUELS DERIVED FROM IT

2020 ◽  
Vol 5 (4) ◽  
pp. 202-209
Author(s):  
Alexander Topal ◽  
◽  
Iryna Holenko ◽  
Luidmyla Haponych ◽  
◽  
...  
Keyword(s):  
Thermal Treatment ◽  
Municipal Solid Waste ◽  
Solid Waste ◽  
Alternative Fuels ◽  
Solid Wastes ◽  
Municipal Solid Wastes ◽  
Local Conditions ◽  
Ecological Requirements ◽  
Clean Technologies

For the municipal solid waste (MSW) to be used in a proper way, it is necessary to implement clean technologies capable of thermal treatment of MSW and RDF in order to produce heat and electricity while meeting current ecological requirements. Nowadays, a number of technologies for MSW/RDF thermal treating are being used worldwide. Among them, the most proven technologies, applicable for industrial introduction, have been considered while analyzing their advantages/ disadvantages accounting for local conditions of Ukraine.

Conditioning of oily sludges with municipal solid wastes incinerator fly ash

1997 ◽  
Vol 35 (8) ◽  
pp. 231-238 ◽  
Author(s):  
Tay Joo Hwa ◽  
S. Jeyaseelan
Keyword(s):  
Heavy Metals ◽  
Fly Ash ◽  
Specific Resistance ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Solid Wastes ◽  
Municipal Solid Wastes ◽  
Capillary Suction ◽  
Optimum Dosage

Conditioning of sludges improves dewatering characteristics and reduces the quantity of sludge to be handled. Anaerobic digested sludge collected from a sewage treatment plant contained 1.8% to 8% oil. The increase of specific resistance and capillary suction time (CST) with increasing oil content observed in these samples indicates the interference of oil in dewatering. It has been found that addition of municipal solid wastes incinerator fly ash decreases the specific resistances and capillary suction times of oily sludges rapidly up to 3% dosage. Beyond 3% fly ash, the decrease is less significant and the solids content in the sludge cake increases. This optimum dosage remains the same for sludges with varying oil contents from 1.8% to 12%. The total suspended solids of filtrate decreases with fly ash dosage but the toxic concentrations of heavy metals increases considerably. However at the optimum dosage of 3%, concentrations of heavy metals are within the limits for discharging into the sewers. The correlations of CST with the dewatering characteristics such as specific resistance, filter yield and corrected filter yield are established. These correlations can be used to obtain a quick prediction on dewaterability.

scholarly journals Municipal Solid Waste Characterization and Landfill Gas Generation in Kakia Landfill, Makkah

2021 ◽  
Vol 13 (3) ◽  
pp. 1462
Author(s):  
Faisal A. Osra ◽  
Huseyin Kurtulus Ozcan ◽  
Jaber S. Alzahrani ◽  
Mohammad S. Alsoufi
Keyword(s):  
Solid Waste ◽  
Energy Recovery ◽  
Solid Waste Management ◽  
Landfill Gas ◽  
Drainage System ◽  
Solid Wastes ◽  
Generation Model ◽  
Gas Generation ◽  
Municipal Solid Wastes ◽  
Makkah City

In many countries, open dumping is considered the simplest, cheapest, and most cost-effective way of managing solid wastes. Thus, in underdeveloped economies, Municipal Solid Wastes (MSW) are openly dumped. Improper waste disposal causes air, water, and soil pollution, impairing soil permeability and blockage of the drainage system. Solid Waste Management (SWM) can be enhanced by operating a well-engineered site with the capacity to reduce, reuse, and recover MSW. Makkah city is one of the holiest cities in the world. It harbors a dozen of holy places. Millions of people across the globe visit the place every year to perform Hajj, Umrah, and tourism. In the present study, MSW characterization and energy recovery from MSW of Makkah was determined. The average composition of solid waste in Makkah city is organic matter (48%), plastics (25%), paper and cardboard (20%), metals (4%), glass (2%), textiles (1%), and wood (1%). In order to evaluate energy recovery potential from solid waste in Kakia open dumpsite landfill, the Gas Generation Model (LandGEM) was used. According to LandGEM results, landfill gas (methane and carbon dioxide) generation potential and capacity were determined. Kakia open dump has a methane potential of 83.52 m3 per ton of waste.

Numerical and experimental analysis of municipal solid wastes gasification process

2015 ◽  
Vol 78 ◽  
pp. 185-195 ◽  
Author(s):  
Nuno Couto ◽  
Valter Silva ◽  
Eliseu Monteiro ◽  
Sandra Teixeira ◽  
Ricardo Chacartegui ◽  
...  
Keyword(s):  
Experimental Analysis ◽  
Solid Wastes ◽  
Municipal Solid Wastes ◽  
Gasification Process
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