scholarly journals Mutagenic properties of modified hydrothermal nanotitania extract

2019 ◽  
Vol 19 (1) ◽  
pp. 159-162
Author(s):  
Ahmad Mukifza Harun ◽  
Noor Baizura Ab Ghani ◽  
Nor Farid Mohd Noor ◽  
Razif Abas ◽  
Mohammad Khursheed Alam
Keyword(s):  
Incubation Period ◽  
Rat Liver ◽  
Ames Test ◽  
Medical Science ◽  
Metabolic Activation ◽  
Mutagenic Effect ◽  
Distilled Water ◽  
Standard Assay ◽  
Serial Dilutions

Backgrounds: The mutagenic properties of modified hydrothermal nanotitania extract were carried out using the Ames test (genotoxicity). Materials and methods: The Ames test was performed on Salmonella strains (TA98, TA100, TA1535, TA1537 and TA 102) which contain mutations in several genes with and without S9 metabolic activation from rat liver using the standard assay. The materials were extracted in distilled water and the serial dilutions of concentration ranging from 313 to 5000 μg/mLwere used after the incubation period of 24 h at 37° C. Results: These results suggested that all tested concentrations of the material extracts did not produce mutagenic effect in all the strains tested. Conclusions: Findings from this study showed that the modified hydrothermal nanotitania extract was non-mutagenic under present conditions. Bangladesh Journal of Medical Science Vol.19(1) 2020 p.159-162

scholarly journals Evaluation of mutagenic activity of the pesticides: actara, sencor, mospilan, pencozeb, fastac in the Ames test

2008 ◽  
Vol 6 (4) ◽  
pp. 29-33 ◽  
Author(s):  
Nazira S Karamova ◽  
Alexandra P Denisova ◽  
Zenon Stasevski
Keyword(s):  
Salmonella Typhimurium ◽  
Rat Liver ◽  
Potato Plant ◽  
Ames Test ◽  
Mutagenic Activity ◽  
Metabolic Activation ◽  
Mutagenic Effect ◽  
Toxic Concentrations ◽  
S9 Fraction

The mutagenic activity of five pesticides actara, sencor, mospilan, pencozeb, fastac widely used for treatment of potato plant lands in Tatarstan was tested in the Ames test. The non toxic concentrations of the pesticides determined in preliminary cytotoxicty test were used in the Ames assay. Pesticides actara, mospilan, pencozeb, fastac did not show mutagenic effect in Salmonella typhimurium TA 100 without rat liver S9 fraction. The weak mutagenic effect of herbicide sencor was established at concentration 1 ug/plate. Metabolic activation in vitro using rat liver S9 fraction decreased the mutagenic activity of sencor and did not alter the mutagenicity rate of the pesticides actara, mospilan, pencozeb and fastac.

scholarly journals Sensitivity of plant and bacterial test systems in detecting of mutagenic effects of oil pollution

2006 ◽  
Vol 4 (1) ◽  
pp. 22-27
Author(s):  
Petimat M Djambetova ◽  
Nina V Reutova
Keyword(s):  
Glycine Max ◽  
Ames Test ◽  
Oil Pollution ◽  
Mutagenic Effect ◽  
Test System ◽  
Test Systems ◽  
Glycine Max L ◽  
Plant Test ◽  
Mutagenic Effects ◽  
By Products

The plant test system soybean (Glycine max. (L.) Merill) line T219 turned out to be more sensitive than standart Ames test for evaluation of the mutagenic effect of soil, contaminated by products of combustion and domestic cottage processing of oil. It is preferable to use plant test systems for such investigations because they are more sensitive, simple and inexpensive in comparison with microbial ones.

Trenbolone growth promotant: covalent DNA binding in rat liver and inSalmonella typhimurium, and mutagenicity in the Ames test

1988 ◽  
Vol 62 (2-3) ◽  
pp. 103-109 ◽  
Author(s):  
W. K. Lutz ◽  
R. Deuber ◽  
M. Caviezel ◽  
P. Sagelsdorff ◽  
U. Friederich ◽  
...  
Keyword(s):  
Dna Binding ◽  
Rat Liver ◽  
Ames Test

Metabolic Activation of Procarcinogens to Genotoxic Products in Cultured Rat Liver Cells

Pathobiology ◽  
1984 ◽  
Vol 52 (6) ◽  
pp. 355-360
Author(s):  
G L. Murison ◽  
S D. Hall ◽  
A M. Lee
Keyword(s):  
Rat Liver ◽  
Metabolic Activation ◽  
Liver Cells ◽  
Rat Liver Cells

scholarly journals Bioactive Sesquiterpene Obtained from Schinus areira L. (Anacardiaceae) Essential Oil

Proceedings ◽  
2019 ◽  
Vol 41 (1) ◽  
pp. 85
Author(s):  
Silvana Rodriguez ◽  
Rosa Ana Sueiro ◽  
Ana Paula Murray ◽  
José Manuel Leiro
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Ex Vivo ◽  
Metabolic Activation ◽  
Mutagenic Effect ◽  
13C Nmr Spectroscopy ◽  
Reversed Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Trolox Equivalent

The essential oils (EOs) from the leaves of Schinus areira and one of its components, globulol, were studied for their antioxidant, antimutagenic and antipromutagenic activities. The chemical composition of the EOs obtained using hydrodistillation was determined using gas chromatography-mass spectrometry (GC-MS), and fractionated using reversed phase high performance liquid chromatography (RP-HPLC). The active compound (16.61%) isolated was identified by comparison of its 1H and 13C NMR spectroscopy with those reported in the literature. The antioxidant activity of the EOs and globulol were determined using two methods: crocin bleaching inhibition (Trolox Equivalent Value, TEV Krel = 1.16 ± 0.11 vs. 1.24 ± 0.22) and scavenging of the DPPH radical (IC50 = 38.75 ± 2.5 μg/mL vs. 5.60 ± 0.9 μg/mL). The antimutagenic and antipromutagenic activities were evaluated in vitro and ex vivo, using the Ames assay with five strains of Salmonella typhimurium with and without exogenous metabolic activation (rat liver fraction S9), against different mutagens. The result determined that globulol and EOs of S. areira at the applied doses do not exhibit any mutagenic effect and showed the highest antioxidant activity.

scholarly journals Antimutagenic activity of raw materials and by-products by production of grape wines

2009 ◽  
Vol 26 (Special Issue) ◽  
pp. S55-S59
Author(s):  
J. Totušek ◽  
D. Lefnerová ◽  
M. Kyseláková ◽  
J. Balík ◽  
J. Veverka ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Ames Test ◽  
Raw Materials ◽  
Experimental System ◽  
Metabolic Activation ◽  
Antimutagenic Activity ◽  
Bacterial Strains ◽  
Methanolic Extracts ◽  
Increased Risk ◽  
By Products

The inhibition of mutagenicity was assessed by Ames test by bacterial strains <i>Salmonella typhimurium</i> TA98 and TA100 using two mutagens and methanolic extracts of healthy fresh berries of blue grapevine varieties – St. Laurent, Portugal, André and white varieties – Chardonnay, Welschriesling, Pinot Gris and berries infected with <i>Botrytis cinerea</i> fungus. As model mutagens, two compounds whose presence in food is real, 2-amino-3-methyl-3H-imidazo-(4.5-f-)-quinoline (IQ), arising from certain heat treatments of meat and acting as indirect mutagen after metabolic activation, and N-nitroso-N-methylurea (MNU) acting as a direct mutagen, were applied. An increased risk of MNU is due to its possible endogenous formation. Fermentation sediment after vinification of the varieties Chardonnay, Welschriesling and André was tested by similar experimental system. All extracts showed strong positive inhibition of mutagenicity, berries infested with Botrytis cinerea also in diluted extracts. Positive inhibition was demonstrated also by fermentation sludge.

A Rapid Procedure to Evaluate the Effect of Pesticides on Nitrification

Weed Science ◽  
1973 ◽  
Vol 21 (5) ◽  
pp. 397-399 ◽  
Author(s):  
R. P. Thorneburg ◽  
J. A. Tweedy
Keyword(s):  
Incubation Period ◽  
Soil Microorganisms ◽  
Nitrification Inhibitor ◽  
Rapid Screening ◽  
Distilled Water ◽  
Laboratory Procedure ◽  
Rapid Procedure ◽  
Short Period

A laboratory procedure for evaluating the effect of pesticides on nitrification in soil proved to be simple to perform, reproducible, and offers a procedure for rapid screening of a large number of chemicals in a short period. The recovery of added nitrate by extracting with distilled water was essentially 100% complete. The conversion of added ammonium to nitrate by the soil microorganisms was nearly complete after the 2-week incubation period. Nitrification in soil treated with several different herbicides and insecticides was determined by our procedure. A nitrification inhibitor, N-Serve3 (2-chloro-6-trichloromethyl pyridine) was included as a standard. None of the herbicides or insecticides inhibited nitrification and the N-Serve completely inhibited nitrification during the 2-week incubation.

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