scholarly journals In vitro anticancer potential and GC-MS analysis of Drimia nagarjunae, an endangered medicinal plant

2015 ◽  
Vol 10 (2) ◽  
pp. 303 ◽  
Author(s):  
Narendranath Alluri ◽  
Mala Majumdar
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Ms Analysis ◽  
Endangered Medicinal Plant ◽  
Colon Cancer Cell Lines ◽  
Total Growth ◽  
First Time

<p>The aim of the present study was to investigate the anticancer activity of <em>Drimia nagarjunae</em> (Liliaceae) extracts against Colo205 human colon cancer cell lines by SRB assay for the first time. The bulbs and leaves of the plant were sequentially extracted using solvents with increasing polarities (hexane&gt; chloroform&gt;ethyl acetate&gt;methanol&gt;water). Ethyl acetate and chloroform bulb extracts showed potent anticancer activity compared to standard, adriamycin. Both the extracts exhibited total growth inhibition of cell at 20.1 µg/mL and 32.1 µg/mL whereas adriamycin shown 33.1 µg/mL and 50% lethal concentration was found to be 61.5 µg/mL and &gt;80 µg/mL respectively. The active extracts were subjected to GC-MS analysis for identification of phytocompounds and it showed seven and ten major compounds respectively. Therefore, the present study demonstrated that <em>D. nagarjunae</em> can be a promising candidate as an anticancer agent.</p><p> </p>

Design, synthesis, and anticancer activity of novel aryl/heteroaryl chalcone derivatives

2016 ◽  
Vol 22 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Ananda Mohan Arasavelli ◽  
Ganapavarapu Veera Raghava Sharma ◽  
Siddaiah Vidavalur
Keyword(s):  
Anticancer Activity ◽  
Coupling Reaction ◽  
Human Colon ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Design Synthesis ◽  
Reference Drug ◽  
Cross Coupling Reaction ◽  
Colon Cancer Cell Lines

AbstractA new series of chalcones 5a–l were synthesized and evaluated for in vitro antiproliferative activity against human colon cancer cell lines. The synthesis of the key intermediate compounds 3a–d was achieved by tetrakis(triphenylphosphine) palladium(II) mediated Suzuki cross coupling reaction. Chalcone 5a shows superior anticancer activity with IC50 value of 21.0 μg/mL compared to the IC50 value of the reference drug doxorubicin at 21.65 μg/mL.

In vitro antiproliferative activity against human colon cancer cell lines of representative 4-thiazolidinones. Part I

2005 ◽  
Vol 15 (17) ◽  
pp. 3930-3933 ◽  
Author(s):  
Rosaria Ottanà ◽  
Stefania Carotti ◽  
Rosanna Maccari ◽  
Ida Landini ◽  
Giuseppa Chiricosta ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antiproliferative Activity ◽  
Human Colon ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines

scholarly journals IN VITRO CYTOTOXIC ACTIVITY OF ISOLATED COMPOUNDS FROM VIBURNUM PUNCTATUM BUCH-HAM EX D. DON

2016 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
A. Renjith Alex ◽  
K. Ilango
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Chloroform Methanol ◽  
Human Colon Cancer Cells

Objective: The main aim of the study was to screen the isolated compounds of Viburnum Punctatum for its in vitro anticancer activity and its percentage viability against HCT 15 (Human Colon Cancer Cells) Cell lines.Methods: Pet ether, Chloroform, Methanol and Aqueous extracts was prepared and assayed for the presence of phytochemicals. Two compounds were isolated from the methanol extract of Viburnum Punctatum by column chromatography such as ME1 (Quercetin) and ME2 (Kaemferol-3-glycoside) characterised by UV, IR, MS, 1H NMR and 13C NMR. The above isolated compounds were subjected to in vitro anticancer activity on HCT 15 cell lines was evaluated by Micro culture Tetrazolium (MTT) assay.Results: ME1 showed significant cytotoxic activity than the ME2 on HCT 15 cells with a percentage viability of 54.60 and 67.18 in the concentration of 10µg/ml and 50µg/ml respectively.Conclusion: On the basis of obtained results, ME1 and ME2 isolated from a methanolic extract of Viburnum Punctatum represent a new group of cytotoxic against HCT 15 Cell lines.

Avenanthramides Inhibit Proliferation of Human Colon Cancer Cell Lines In Vitro

2010 ◽  
Vol 62 (8) ◽  
pp. 1007-1016 ◽  
Author(s):  
Weimin Guo ◽  
Lin Nie ◽  
Dayong Wu ◽  
Mitchell L. Wise ◽  
F. William Collins ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Cancer Cell Lines ◽  
Colon Cancer Cell ◽  
Human Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines

scholarly journals Nimesulide Based Novel Glycolamide Esters: Their Design, Synthesis, and Pharmacological Evaluation

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Kavitha Kankanala ◽  
Vangala Ranga Reddy ◽  
Yumnam Priyadarshini Devi ◽  
Lakshmi Narasu Mangamoori ◽  
Khagga Mukkanti ◽  
...  
Keyword(s):  
Cancer Cell Line ◽  
Human Colon ◽  
Colon Cancer Cell Line ◽  
Cytotoxic Activities ◽  
Step Method ◽  
Human Colon Cancer ◽  
Cytotoxic Effects ◽  
Design Synthesis ◽  
First Time

The nimesulide based novel glycolamide esters were designed and synthesized for the first timeviaa three-step method starting from nimesulide. Structures of the synthesized compounds were confirmed by spectroscopic analysis. All the synthesized compounds were examined for their cytotoxic effectsin vitro,some of which showed significant cytotoxic activities against HCT-15 human colon cancer cell line.

scholarly journals Morphomolecular identification, metabolic profile, anticancer, and antioxidant capacities of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 isolated from Egyptian remote cave

2021 ◽  
Author(s):  
Waill A. Elkhateeb ◽  
Walaa S. A. Mettwally ◽  
Shireen A. A. Saleh ◽  
Walid Fayad ◽  
Ibrahim M. Nafady ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Colon Cancer ◽  
Ethyl Acetate ◽  
Human Breast Cancer ◽  
Mcf7 Cell ◽  
Human Colon ◽  
Human Breast ◽  
Human Colon Cancer ◽  
Breast Cancer Mcf7 Cell

Abstract Searching for remote locations to screen for microorganisms, identify their metabolites, and investigate their bioactivities against lethal diseases such as cancer is of critical importance. In the current study, two fungal strains where isolated from a remote cave in Asyut governorate, Egypt. These isolates were morphologically and molecularly identified through sequencing their ITS region as Penicillium sp. NRC F1, and Penicillium sp. NRC F16. Investigating the metabolic profiles of the silylated ethyl acetate extracts of these fungi through conducting GC-Ms analysis revealed presence of 114 compounds belonging to different chemical classes. On the other hand, studying the in vitro bioactivity of both extracts showed moderate antioxidant activities. Penicillium sp. NRC F1 extract exhibited higher DPPH scavenging activity (74.41 ± 0.59%) at concentration of 200 µg/ml, in comparison with that exerted by Penicillium sp. NRC F16 extract at the same concentration (65.58 ± 1.55%). Moreover, studying the cytotoxicity of extracts against human colon cancer (HCT116), and human breast cancer (MCF7) cell lines revealed that cytotoxicity of both extracts was dose dependent. Promising cytotoxic effect was achieved against human colon cancer HCT116 using 200 µg/ml of Penicillium sp. NRC F1 extract (95.72 ± 1.13 % cytotoxicity), while Penicillium sp. NRC F16 ethyl acetate extract caused a cytotoxicity of 95.43 ± 1.4 %. Similarly, investigating the in vitro cytotoxicity of the extracts against human breast cancer MCF7 cell line resulted in observing promising activity of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 extracts, and they exhibited 97.29 ± 0.61 %; and 97.08 ± 1.07 % cytotoxicity, respectively. Results from this study nominate those strains as promising isolates and encourage for conducting further in vivo investigations to evaluate their potency.

scholarly journals Inhibitory Effect of CAPE and Kaempferol in Colon Cancer Cell Lines—Possible Implications in New Therapeutic Strategies

2019 ◽  
Vol 20 (5) ◽  
pp. 1199 ◽  
Author(s):  
Liviuta Budisan ◽  
Diana Gulei ◽  
Ancuta Jurj ◽  
Cornelia Braicu ◽  
Oana Zanoaga ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cell Proliferation ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Colon ◽  
Natural Compounds ◽  
Colon Cancer Cell ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines

Background: Phytochemicals are natural compounds synthesized as secondary metabolites in plants and represent an important source of molecules with therapeutic applications. Attention is accorded to their potential in anti-cancer therapies as single agents or adjuvant treatment. Herby, we evaluated the in vitro effects of a panel of natural compounds with focus on caffeic acid phenethyl ester (CAPE) and Kaempferol for the treatment of human colon cancer. Methods: We exposed two human colon cancer cell lines, RKO and HCT-116, followed by functional examination of cell viability, cell proliferation and invasion, cell cycle, apoptosis, and autophagy. Modifications in gene expression were investigated through microarray and detection of existing mutations and finding of new ones was done with the help of Next Generation Sequencing (NGS). Results: Both CAPE and Kaempferol inhibit cell proliferation, motility and invasion, and stimulate apoptosis and autophagy, concomitant with modifications in coding and noncoding genes’ expression. Moreover, there are pathogenic mutations that are no longer found upon treatment with CAPE and Kaempferol. Conclusions: Our findings indicate that CAPE and Kaempferol have the ability to negatively influence the development and advancement of colon cancer in vitro by specifically altering the cells at the molecular level; this activity can be exploited in possible adjuvant therapies once the optimal dose concentration with minimal side effects but with cancer inhibitory activity is set in vivo.

Monoclonal Antibodies to αDna Polymerase as a Marker of Cell Proliferative Activity

1987 ◽  
Vol 2 (1) ◽  
pp. 25-30
Author(s):  
Angela Alama ◽  
Silvano Ferrini ◽  
Marco Gaddi ◽  
Angelo Nicolin
Keyword(s):  
Colon Cancer ◽  
Monoclonal Antibodies ◽  
Kinetic Studies ◽  
Human Colon ◽  
Peripheral Blood Lymphocytes ◽  
Growth Fraction ◽  
Human Colon Cancer ◽  
Colon Cancer Cell Lines ◽  
Cell Proliferative Activity

A hybridoma cell line (5F) secreting monoclonal antibodies directed to αDNA polymerase has been developed. Kinetic studies on peripheral blood lymphocytes stimulated with mitogen and human colon cancer cell lines established in vitro were made by the two autoradiographic techniques of Thymidine Labelling Inde and Primer-dependent αDNA polymerase Labelling Index and the immunoperoxidase assay (PAP) with monoclonal antibody to αDNA polymerase. We demonstrated the exclusively intranuclear presence of αDNA polymerase in lymphocytes induced to proliferate and actively growing colon cancer cells in contrast with the cytoplasmic distribution of the enzyme in resting stage populations. The feasibility of using monoclonal antibodies to αDNA polymerase to determine cell growth fraction was evaluated.

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