scholarly journals In vitro Bioactivities of Aerial Parts of Dioscorea alata

2018 ◽  
Vol 20 (2) ◽  
pp. 200-204
Author(s):  
Md Akbar Hossain ◽  
Md Enamul Haque ◽  
Md Hassan Kawsar ◽  
Md Sohel Rana
Keyword(s):  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Total Phenolic ◽  
Dioscorea Alata ◽  
Hypotonic Solution ◽  
Aerial Parts ◽  
Dpph Method

The methanol extract of aerial parts of Dioscorea alata and its organic and aqueous soluble materials were subjected to screenings for antioxidant, cytotoxic, thrombolytic, membrane stabilizing and antimicrobial activities. The polyphenol content and DPPH free radical scavenging assay were conducted to evaluate the antioxidant activity. The total phenolic content was found to vary for different test samples ranging from 23.69 ± 0.25 to 26.60 ± 0.22 mg of GAE/gm of dried extract. In DPPH method the chloroform soluble fraction (CSF) of D. alata revealed the highest free radical scavenging activity with IC5025.33 ± 0.05 μg/ml. The petroleum ether soluble fraction (PESF) exhibited highest cytotoxic potential having LC50 value of 6.1 μg/ml. During the membrane stabilizing assay, the aqueous soluble fraction (AQSF) inhibited 31.91% and 47.55% haemolysis of RBC in hypotonic solution- and heat-induced method, respectively. In thrombolytic assay, the chloroform soluble fraction (CSF) displayed 40.45% clot lysis. On the other hand the carbon tetrachloride (CTCSF) and chloroform soluble fraction (CSF) revealed mild to moderate antimicrobial activity with zone of inhibition ranging from 8 to 12 mm.Bangladesh Pharmaceutical Journal 20(2): 200-204, 2017

scholarly journals Wild Cinchona (Neolamarckia cadamba) – Bioactivities of a Medicinal Plant of Bangladesh

2016 ◽  
Vol 19 (1) ◽  
pp. 32-36
Author(s):  
Faiza Tahia ◽  
Mohidur Rahman ◽  
Marzia Bilkiss ◽  
Md Al Amin Sikder ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Methanolic Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Vincristine Sulphate

The methanol extract of bark of Neolamarckia cadamba (Roxb.) and its organic and aqueous soluble partitionates were subjected to assays for antioxidant, cytotoxic, thrombolytic, membrane stabilizing, antimicrobial and analgesic activities. In the DPPH free radical scavenging assay, the pet ether and carbon tetra chloride soluble partitionate of the methanolic extract demonstrated the highest free radical scavenging activity with IC50 values of 60.46 ?g/ml and 78.58 ?g/ml, respectively. On the other hand, the carbon tetrachloride and pet ether soluble fractions displayed the potential lethality to brine shrimps, having LC50 of 7.24 and 7.89 ?g/ml, as compared to standard vincristine sulphate (LC50 value of 0.45 ?g/ml). During assay for thrombolytic property, the carbon tetrachloride and aqueous soluble materials revealed 66.36 % and 64.25 % clot lysis of human blood, respectively. In the membrane stabilizing assay, the carbon tetrachloride soluble fraction inhibited 86.79 % haemolysis of human RBCs in hypotonic solution-induced condition, while the aqueous soluble partitionate inhibited 92.39 % haemolysis of RBCs in the heat-induced condition. The crude methanolic extract of bark of N. cadamba showed significant central and peripheral analgesic activity at both 200 and 400 mg/kg body weight, whereas its chloroform soluble fraction mildly inhibited the growth of test microorganisms. Therefore, our studies suggest that wild cinchona should be subjected to extensive phytopharmacological investigation.Bangladesh Pharmaceutical Journal 19(1): 32-36, 2016

scholarly journals In vitro Bioactivities of Three Reputed Medicinal Plants of Bangladesh

2015 ◽  
Vol 17 (2) ◽  
pp. 147-150 ◽  
Author(s):  
Abu Bakar Siddique ◽  
Ridwan Islam ◽  
Md Al Amin Sikder ◽  
Ridwan Bin Rashid ◽  
AKM Nawshad Hossian ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Free Radical ◽  
Soluble Fraction ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Acetyl Salicylic Acid ◽  
Total Phenolic ◽  
Hypotonic Solution

The study was designed to evaluate the preliminary biological activities of crude methanol extracts of the leaf of Justicia gendarussa, Sansevieria trifasciata and Hydnocarpus kurzii and their organic soluble partitionates. The polyphenol content was determined spectrophotometrically and expressed as gallic acid equivalents. The total phenolic content was found to vary for different test samples ranging from 1.45 ± 0.25 mg to 40.73 ± 0.22 mg of GAE/ gm of dried extract. The antioxidant potential was evaluated by DPPH free radical scavenging assay using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the assay, the CSF of H. kurzii and the PESF of J. gendarussa revealed the highest free radical scavenging activity with IC50 values 3.25 ± 0.05 ?g/ml and 24.68 ± 0.26 ?g/ml, respectively. The brine shrimp lethality bioassay was utilized to evaluate the cytotoxicity. The chloroform soluble fraction (CSF) and methanol extract (ME) of J. gendarussa exhibited strong cytotoxicity with LC50 values of 0.002 ?g/ml and 0.06 ?g/ml, respectively. The membrane stabilizing activity was assessed by evaluating hemolysis of RBC in hypotonic solution and was compared with acetyl salicylic acid. On the other hand, the chloroform soluble fraction of J. gendarussa and aqueous soluble materials of S. trifasciata produced 75.60% and 75.0% inhibition of hemolysis of RBC, respectively as compared to 77.9% inhibited by acetyl salicylic acid (0.10 mg/ml). DOI: http://dx.doi.org/10.3329/bpj.v17i2.22331 Bangladesh Pharmaceutical Journal 17(2): 147-150, 2014

scholarly journals Bioactivities of Malvaviscus arboreus var. drummondii and Phyllanthus reticulatus Poir.

2015 ◽  
Vol 13 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Zokhroof Yeasmin ◽  
Sharif Tanvir ◽  
Tasnuva Sharmin ◽  
Ridwan Bin Rashid ◽  
Md Al Amin Sikder ◽  
...  
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Hypotonic Solution ◽  
Activity Assay ◽  
Vincristine Sulphate ◽  
Crude Methanol Extract

The study was designed to evaluate the bioactivities of crude methanol extracts of leaves of Malvaviscus arboreus and Phyllanthus reticulatus along with their pet-ether, carbon tetrachloride, chloroform and aqueous soluble partitionates. The antioxidant potential was evaluated by DPPH and Folin-Ciocalteau reagents using butylated hydroxytolune (BHT) and ascorbic acid as standards. In the DPPH free radical scavenging assay, the crude methanol extract of M. arboreus revealed the highest free radical scavenging activity with IC50 values 6.47 ± 0.78 ?g/ml. In brine shrimp lethality bioassay, the chloroform soluble materials of M. arboreus displayed the highest cytotoxic potential having LC50 values 2.73 ± 0.36 ?g/ml, whereas the standard vincristine sulphate exhibited an LC50 value of 0.45 ?g/ml. In the thrombolytic activity assay, the pet-ether soluble fractions of M. arboreus and P. reticulatus revealed 55.60 ± 0.48 % and 18.99 ± 0.84% clot lysis, respectively. In the membrane stabilizing activity assay, the chloroform soluble fraction of M. arboreus inhibited heat- and hypotonic solution- induced haemolysis of RBCs by 54.60 ± 0.81 % and 66.96 ± 0.84 % as compared to 57.06 % and 72.79 % inhibition by acetyl salicylic acid (0.10 mg/ml), respectively. On the other hand, the pet-ether soluble materials of crude methanol extract of P. reticulatus demonstrated 57.11 ± 0.57 % and 70.62 ± 0.22 % inhibition of heat- and hypotonic solution- induced hemolysis, respectively. DOI: http://dx.doi.org/10.3329/dujps.v13i2.21892 Dhaka Univ. J. Pharm. Sci. 13(2): 143-147, 2014 (December)

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Antioxidant, Membrane Stabilizing and Cytotoxic Activities of Cissus adnata (Roxb.)

2016 ◽  
Vol 15 (1) ◽  
pp. 69-71 ◽  
Author(s):  
Ridwan Bin Rashid ◽  
Farhan Nur Towsif ◽  
Farhana Ahmed Bushra ◽  
Faiza Tahia
Keyword(s):  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cytotoxic Activities ◽  
Hypotonic Solution ◽  
Lc50 Value ◽  
Whole Plant ◽  
Vincristine Sulphate

The methanol extracts of whole plant of Cissus adnata (Roxb.) and their organic and aqueous soluble partitioning materials were evaluated for antioxidant, cytotoxic and membrane stabilizing properties. In the DPPH free radical scavenging assay, the aqueous extract of whole plant of C. adnata displayed the highest free radical scavenging activity with IC50 value of 4.17 ?g/ml, while the pet ether soluble fraction showed significant lethality to brine shrimps having LC50 value of 1.02 ?g/ml, as compared to the standard vincristine sulphate (LC50 value of 0.44 ?g/ml). In the evaluation for membrane stabilizing property, the pet ether soluble fraction of methanol extract of the whole plant inhibited 67.43 % and 56.60 % hypotonic solution- and heat-induced haemolysis of RBCs, respectively.Dhaka Univ. J. Pharm. Sci. 15(1): 69-71, 2016 (June)

scholarly journals Preliminary Free Radical Scavenging, Brine Shrimp Lethality, Antimicrobial and Thrombolytic Activities of Aganosma dichotoma (Roth) K. Schum.

2015 ◽  
Vol 17 (2) ◽  
pp. 177-181
Author(s):  
Sanjoy Chandra Dey ◽  
Mohammd Firoz Khan ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Brine Shrimp ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Pharmaceutical Journal ◽  
Free Radical Scavenging ◽  
Clot Lysis ◽  
Brine Shrimp Lethality

Bangladesh is a good repository of natural products. Numerous plants are available to facilitate the traditional treatments. Proper scientific evaluations are essential to explore the plant derived drugs. With this view, the crude methanol extract of leaves of Aganosma dichotoma (Roth) K. Schum. and its Kupchan fractions were screened for free radical scavenging, brine shrimp lethality, antimicrobial and thrombolytic activities. Among all extractives, the chloroform soluble fraction demonstrated the highest free radical scavenging activity with IC50 value 18.21 ?g/ml. Moreover, the chloroform soluble fraction showed significant brine shrimp lethality having LC50 value of 3.98 ?g/ml. On the other hand, the petroleum ether, carbon tetrachloride and chloroform soluble materials revealed mild to moderate antimicrobial activity with the zone of inhibition ranging from 7 to 14 mm. In the thrombolytic assay, the carbon tetrachloride soluble partitionate showed the highest clot lysis (30.48%). This is the first report of the comprehensive investigations of different extractives of A. dichotoma for the abovementioned bioassays. DOI: http://dx.doi.org/10.3329/bpj.v17i2.22337 Bangladesh Pharmaceutical Journal 17(2): 177-181, 2014

Comparative Biochemical Analysis of Secondary Metabolites in Commonly Grown Legume Varieties

2019 ◽  
Author(s):  
Savita Rani ◽  
Sukriti Nehra ◽  
M Khabiruddin ◽  
Shubham Lamba
Keyword(s):  
Free Radical ◽  
Secondary Metabolites ◽  
Seed Coat ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Dpph Method ◽  
Whole Seed

In this research study, quantitative determination of phytoconstituents in seed coat, dehusked raw dal, cooked dal and whole seed of locally grown pulses viz. pigeonpea, lentil, desi and kabuli chickpea, and ricebean was carried out. Among the four treatments of all the pulses, highest yield in methanol was found in ricebean. Total phenolic content (mg GAEg-1) varied from 1.72-103.62 in all of the extracts. Similarly, flavonoids (mg CAEg-1) ranged from 1.13 to 33.66 which also found to be reduced in dehulled and cooked extracts. o-Dihydric phenols, hydrophobic and hydrophilic phenols followed order as: seed coat> whole seed> raw dal> cooked dal. Results obtained by DPPH method showed that free radical scavenging capacities ranged from 53.80 to 92.00% while antioxidant activity ranged from 51.00 to 78.60% by FTC method. A highly significant correlation (plessthan0.05) between antioxidant activities/free radical scavenging efficiencies and phenolic contents as well as flavonoids was observed in most of the extracts under study. The results concluded that the pulse seed extracts may be valuable natural source of secondary metabolites for nutraceutical industry.

scholarly journals Antioxidant and Membrane Stabilizing Activities of Bark of Sonneratia apetala

2016 ◽  
Vol 19 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Md Emdadul Hasan Mukul ◽  
Mohammad Salim Hossain ◽  
Sayed Koushik Ahamed ◽  
Pankaj Debnath ◽  
Mariyam Akter
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Hypotonic Solution ◽  
Sonneratia Apetala ◽  
Reducing Power Assay

This study was carried out to investigate the antioxidant activity in terms of free radical scavenging capacity and membrane stabilizing ability of methanol extract of bark of Sonneratia apetala. The total phenol content of the extract was measured by Folin-Ciocalteu reagent. The antioxidant potential was investigated using 2,2 diphenylpicrylhydrazyl (DPPH), reducing power assay and chelating power determination. The membrane stabilizing capacity was assessed by monitoring the hypotonic solution - and heat-induced haemolysis of human erythrocytes. The total phenolic content was found 50.75 mg/gm of gallic acid equivalent. The extract exhibited significant antioxidant activity in DPPH free radical scavenging assay with IC50 value of 81.42 µg/ml as compared to the standard, BHT (IC50=42.56 µg/ml), Fe2+ ion reducing power assay and chelating power ability. In case of assay for chelating ability, EDTA represented 98.76% chelation while the plant extract showed 82.83% at concentration of 100 ?g/ml. In addition, the methanol extract of bark of this plant was found to moderately inhibit the haemolysis of human erythrocyte. In conclusion, the methanol extract of S. apetala could be valuable candidate for future development for antioxidant activity.Bangladesh Pharmaceutical Journal 19(2): 147-151, 2016

scholarly journals Antioxidant, Antimicrobial, Anti-diarrheal and Analgesic Activities of Diospyros malabarica (Desr.) Kostel

2019 ◽  
Vol 22 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Md Moniruzzaman ◽  
Md Ruhul Kuddus ◽  
AM Sarwaruddin Chowdhury ◽  
Mohammad A Rashid
Keyword(s):  
Free Radical ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Tail Flick ◽  
Dependent Manner ◽  
Analgesic Activities

The present study was designed to evaluate the antioxidant, antimicrobial, antidiarrheal and analgesic activities of methanol extract of Diospyros malabarica (Desr.) Kostel buds growing in Bangladesh. The total phenolic content of methanol extract of D. malabarica and its Kupchan fractions was determined and expressed in gallic acid equivalent (GAE). In the DPPH free radical scavenging assay, the aqueous soluble fraction of the methanol extract of D. malabarica revealed maximum free radical scavenging activity having IC50 value of 6.17 μg/ml. In antimicrobial assay, all the test samples displayed prominent antimicrobial activity against the test organisms under in vitro conditions. Among these, the carbon tetrachloride soluble fraction was found to exhibit the highest activity against Bacillus cereus, Salmonella Typhi, Salmonella Paratyphi, and Candida albicans with the zone of inhibition as 32, 30, 28 and 30 mm, respectively. In evaluation of antidiarrheal activity, the D. malabarica extracts showed significant anti-diarrheal potential in a dose dependent manner. During the evaluation of analgesic activity by radiant heat tail-flick method, the plant extract at 400 mg/kg b.w. exhibited highest elongation (373.04%) as compared to morphine (472.48%). On the other hand, in acetic acid-induced writhing test, the extract at 200- and 400-mg/kg b.w. showed 61.11 and 66.67% inhibition of writhing in mice model, respectively as compared to 68.06% inhibition produced by the standard diclofenac-Na. The findings of this study justify some of the traditional uses of D. malabarica and reveal the bioactivity of the plants. Further studies are required to isolate and identify the bioactive compounds. Bangladesh Pharmaceutical Journal 22(1): 27-33, 2019

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