scholarly journals Isolation and Characterization of Malasezzia Species from Dandruff Samples and Determination of its Sensitivity Towards Antifungal Agents

2019 ◽  
Vol 22 (2) ◽  
pp. 146-152
Author(s):  
Kohinur Begum ◽  
Farjahan Nur ◽  
Mashmum Shababa Shahid
Keyword(s):  
Antifungal Agents ◽  
Pharmaceutical Journal ◽  
Skin Condition ◽  
Biochemical Tests ◽  
Malassezia Species ◽  
Isolation And Characterization ◽  
Diffusion Technique ◽  
Malassezia Spp ◽  
Etiological Agents

Dandruff is a perennial skin condition manifesting as inflammation and physiologic scaling on the scalp. Malassezia species are the part of the skin microflora of humans and the etiological agents of dandruff. Malassezia comprises of lipophilic and non-lipophilic yeasts. At present topical azoles are used for the treatment of dandruff. This study was conducted to isolate and identify the Malassezia spp. from dandruff samples and to determine their responsiveness towards antifungal agents such as fluconazole, ketoconazole, miconazole and nystatin. Malassezia spp. was isolated using selective media and identified by biochemical tests and microscopic examination. Antifungal activities were assessed by the Kirby Bauer method using well diffusion technique. Total fifteen Malassezia spp. including M. furfur, M. pachydermatis, M. sympodialis and M. globosa were identified from twenty five dandruff samples. Among them, M. furfur was most prevalent (66.67%) followed by M. pachydermatis (13.33%), M. sympodialis (13.33%) and M. globosa (6.67%). All strains were sensitive to the antifungal agents but to different extent. Fluconazole and ketoconazole proved to be the most effective agents against these microorganisms. Bangladesh Pharmaceutical Journal 22(2): 146-152, 2019

scholarly journals CLINICAL SIGNIFICANCE OF DETERMINATION OF IGE ANTIBODIES TO STAPHYLOCOCCUS AUREUS AND MALASSEZIA SPECIES IN SERUM OF CHILDREN WITH ATOPIC DERMATITIS

2018 ◽  
Vol 63 (4) ◽  
pp. 84-88
Author(s):  
T. S. Okuneva ◽  
M. V. Kushnareva ◽  
A. N. Pampura ◽  
E. E. Varlamov ◽  
N. G. Konyukova
Keyword(s):  
Staphylococcus Aureus ◽  
Atopic Dermatitis ◽  
Clinical Significance ◽  
Specific Ige ◽  
Frequency Of Occurrence ◽  
Ige Antibodies ◽  
Malassezia Species ◽  
Specific Ige Antibodies ◽  
Malassezia Spp

We studied the frequency of occurrence and concentration of allergen-specific IgE antibodies (asIgE) to enterotoxins A, B, C and TSST-1 of Staphylococcus aureus and Malassezia spp. in the serum of children with atopic dermatitis in the acute period of the disease. The determination of asIgE levels was performed in 84 children aged from 5 months to 17 years using the immunochemiluminescent method on the UniCAP 100 analyzer (Phadia, Sweden). The level of asIgE was evaluated on a scale of 6 classes.The sensitization to S. aureus enterotoxins was detected in 29 children with аsIgE concentration from 0.35 to 25.8 kU/L (I–IV classes). In 16 children were observed 8 polysensitization options to S. aureus allergens. Combinations of asIgE to A + B and A + B + C enterotoxins were most common – in every fourth and fifth child,respectively. Sensitization to Malassezia spp. was observed in 41 children with concentration from 0.38 to 98.9 KU/L (I–V classes). Polysensitization to both S. aureus and Malassezia spp. allergens was observed in one of two children. A higher degree of sensitisation to Malassezia spp allergens has been established compared to S. aureus.Conclusion. To improve the specific diagnostics of atopic dermatitis, it is advisable to examine the serum for a complete panel of recombinant S. aureus enterotoxins in combination with the determination of asIgE to Malassezia spp. 

Isolation and characterization of novel mucoadhesive biomaterial from Phoenix dactylifera

2012 ◽  
Vol 1 (8) ◽  
pp. 205-208
Author(s):  
Abhijeet Ojha ◽  
NV Satheesh Madhav
Keyword(s):  
Research Work ◽  
Pharmaceutical Journal ◽  
Phoenix Dactylifera ◽  
Isolation And Characterization ◽  
The Family ◽  
Capra Aegagrus ◽  
Cylinder Method ◽  
Bio Material

Phoenix dactylifera belongs to the family Arecaceae. The current aim of our research work is to isolate bio-material from the fruit pulp of Phoenix dactylifera and evaluate its mucoadhesivity. The bio- material was isolated by simple economical process. The isolated biomaterial was subjected for determination of solubility, colour changing point, viscosity, surface tension, pH and chemical tests. The mucoadhesivity of the biomaterial was assessed by shear stress method and rotating cylinder method using Capra aegagrus labium and intestine as mucosal substrates. The results were compared with HPMC and sodium CMC. The research study revealed that the biomaterial from Phoenix dactylifera exhibits promising inbuilt mucoadhesivity. So it can serve as a powerful natural mucoadhesant and may be used to develop mucoadhesive transmucosal drug delivery systems.DOI: http://dx.doi.org/10.3329/icpj.v1i8.11251 International Current Pharmaceutical Journal 2012, 1(8): 205-208  

Identification of Malassezia Species in Seborrheic Dermatitis Patients by CHROM Agar-Medium

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Mohamed Abd Alnaeem Sallam ◽  
Mohamed Taha Mahmoud El-sayed ◽  
Ahmed Abd Elfattah Afify ◽  
Mustafa Nooruldeen Abdulqader Mustafa
Keyword(s):  
Seborrheic Dermatitis ◽  
Age Groups ◽  
Skin Condition ◽  
Conclusive Evidence ◽  
University Hospital ◽  
Malassezia Species ◽  
The Face ◽  
Malassezia Spp ◽  
Erythematous Plaques

Abstract Background Seborrheic dermatitis (SD) is a subacute or chronic superficial inflammatory skin condition, characterized by pruritic, erythematous plaques with greasy, yellow-gray scales, which appear on areas rich in sebaceous glands such as the face, scalp, upper chest, and back. Aim of the Work To identify Malassezia species that are involved in the development of SD in different body sites (face, scalp and trunk) and in different age groups, by using Chrom agar. Patients and Methods Forty nine patients with seborrheic dermatitis attending dermatology outpatient clinic in Ain shams university hospital the samples collected from December 2018 to June 2019. Results In this study, the most affected areas were the scalp and other sites as face and ears, which is concordant with the majority of studies worldwide. The role of sex in propensity to the development of Malassezia spp. Infection in SD is still unclear. Conclusion The distribution of Malassezia spp. on healthy and diseased skin shows significant variation and differences, although our knowledge of Malassezia yeasts has increased tremendously during the last two decades, their pathological roles remain ambiguous, and there is currently no conclusive evidence that any given species is responsible for a specific disease.

scholarly journals Phenotypic Identification and Antifungal Susceptibility of Two Rhodotorula Species Isolated from Dandruff Samples

2020 ◽  
Vol 19 (2) ◽  
pp. 185-190
Author(s):  
Kohinur Begum ◽  
Mashmum Shababa Shahid ◽  
Sanzida Ferdoush ◽  
Farjahan Nur
Keyword(s):  
Antifungal Agents ◽  
Antifungal Susceptibility ◽  
Colony Morphology ◽  
Disk Diffusion ◽  
Biochemical Tests ◽  
Selective Media ◽  
Phenotypic Identification ◽  
Diffusion Technique ◽  
Rhodotorula Species ◽  
Systemic Infections

Rhodotorula are environmental yeasts originally considered non-pathogenic in nature. However, over the last three decades, different species of this yeast have established themselves as pathogens in humans, causing systemic infections among the immunocompromised population. In this study, Rhodotorula species were isolated from dandruff samples using selective media by observing colony morphology and color. The isolates were later identified via biochemical tests and microscopic examinations. In addition, the sensitivity of the isolates to the three antifungal agents, namely ketoconazole, nystatin and fluconazole were tested by using the disk diffusion technique. On completion of the tests, only two species of Rhodotorula were identified from 35 dandruff samples and designated as R1 and R3. Both isolates displayed sensitivity towards ketoconazole and nystatin. No antifungal sensitivity was documented against fluconazole. This study gave preliminary indication of the presence of Rhodotorula species in dandruff samples, and its sensitivity towards antifungal agents. Dhaka Univ. J. Pharm. Sci. 19(2): 185-190, 2020 (December)

scholarly journals CHARACTERIZATION OF TYPICAL AND ATYPICAL MALASSEZIA SPP. FROM CATTLE AND DOG BY RANDOM AMPLIFIED POLYMORPHIC DNA ANALYSIS

2009 ◽  
Vol 76 (2) ◽  
pp. 157-164
Author(s):  
E.R. Duarte ◽  
J.C.P. Resende ◽  
J.S. Hamdan
Keyword(s):  
Dna Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Biochemical Tests ◽  
Malassezia Species ◽  
Random Amplification ◽  
Dna Pattern ◽  
Malassezia Spp ◽  
The One ◽  
Genetic Profiles

ABSTRACT There are few numbers of biochemical tests for specie classification in the genus Malassezia and these can to fail in the identification of the atypical isolates. In this study, typical and atypical isolates were analysed by random amplification of polymorphic DNA (RAPD) to compare with biochemical-physiological characteristics of the Malassezia species from bovine and canine ears. RAPD band patterns using OPA4 primer clustered all isolates according its biochemicalphysiological characteristics in the species from cattle and dog. Malassezia nana and M. sympodialis isolates were sub-clustered in separated sub-branches and both were from a different branch of the other species. The DNA pattern of the two atypical lipid-dependent M. pachydermatis strains was similar with of other typical strains but it did not show the one specific band of 200bp. Future studies in the specific RAPD bands of genetic profiles can be important to corroborate the identification of typical and atypical isolates of the genus Malassezia.

Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia

2020 ◽  
Vol 14 (1) ◽  
pp. 4
Author(s):  
RUHUL KHALILA ◽  
Lenni Fitri ◽  
SUHARTONO SUHARTONO
Keyword(s):  
Sampling Method ◽  
Bacterial Isolate ◽  
Hot Spring ◽  
Thermophilic Bacteria ◽  
Cellulolytic Enzyme ◽  
Biochemical Tests ◽  
Cellulase Enzymes ◽  
Isolation And Characterization

Cellulase enzymes can be isolated from thermophile bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers,. The sampling method in this research was conducted by a purposive sampling at temperature of 70 oC, 60 oC and 50 oC. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic bacteria isolated recovered at 70 oC, five isolates at 60 oC, and seven isolates at 50 oC. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values ​​were obtained from isolates ISB75; ISB64; ISB52; ISB54; ISB56 that were 1.23; 2.22; 1.39; 1.59; 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.

In vitro efficacy of topical antifungal agents on Malassezia species.

1991 ◽  
Vol 53 (1) ◽  
pp. 144-151 ◽  
Author(s):  
Mamoru YOKOO ◽  
Tadashi ARIKA ◽  
Yoshiro SOH
Keyword(s):  
Antifungal Agents ◽  
Malassezia Species

Nonproteolyzed Form of DNA-Polymerase ε from T-Cell Spontaneous Lymphoma of Sprague-Dawley Inbred Rat: Isolation and Characterization

1998 ◽  
Vol 63 (5) ◽  
pp. 723-731 ◽  
Author(s):  
Gabriel Birkuš ◽  
Pavel Kramata ◽  
Ivan Votruba ◽  
Berta Otová ◽  
Miroslav Otmar ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Sedimentation Coefficient ◽  
Isolation Procedure ◽  
Sprague Dawley ◽  
Nucleotide Analogs ◽  
Isolation And Characterization ◽  
Inbred Rats ◽  
Catalyzed Reactions ◽  
Inbred Rat

Using a simple isolation procedure and selective assay for the determination of enzyme activity the nonproteolyzed and proteolyzed form of DNA-polymerase ε (pol ε and pol ε*) from the lymphoma of Sprague-Dawley inbred rats were purified. Nonproteolyzed pol ε is composed of two subunits (240 000 and 50 000) with sedimentation coefficient 10.5 S, while the subunit composition of pol ε* was 145 000 and 73 000. Estimated Km values for dATP and dGTP as well as Ki values for acyclic nucleotide analogs (PMEApp, HPMPApp and PMEDAPpp) in pol ε and pol ε* catalyzed reactions have shown that a proteolysis probably does not affect pol ε binding site for dNTPs. Both enzymes (pol ε and pol ε*) possess 3'-5'-exonuclease activity with different Km for 3'-OH end of template poly dA-oligo dT18 (1.6 μmol/l and 0.36 μmol/l, respectively).

scholarly journals Isolation and Characterization of a Bacterial Strain Enterobacter cloacae (Accession No. KX438060.1) Capable of Degrading DDTs Under Aerobic Conditions and Its Use in Bioremediation of Contaminated Soil

2021 ◽  
Vol 14 ◽  
pp. 117863612110242
Author(s):  
Sonal Suman ◽  
Tanuja
Keyword(s):  
Contaminated Soil ◽  
Growth Parameters ◽  
Chemical Properties ◽  
Enterobacter Cloacae ◽  
Maximum Growth ◽  
Bacterial Degradation ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
16S Rna ◽  
Degrading Bacteria

DDT is one of the most persistent pesticides among all the different types of organo-chlorine pesticides used. Among all the degradation methods, bacterial degradation of DDT is most effective. The present study was conducted to isolate different bacteria present in waste samples which have the ability to degrade DDT present in the soil in the minimum possible period of time and to observe the effect of different physical and chemical properties of the soil samples. Many pesticide degrading bacteria were isolated and identified through cultural, biochemical tests and further identified by 16S RNA sequencing method. The most potent strain DDT 1 growth in mineral salt medium supplemented with DDT as the only source of carbon (5-100 PPM) and was monitored at an optical density of 600 nm. The growth parameters at different physio-chemical conditions were further optimized. The result showed that Enterobacter cloacae had maximum growth in 15 days. FTIR analysis of the residual DDT after 15 days incubation showed that Enterobacter cloacae was able to degrade pesticide into its further metabolites of DDD, DDE, DDNU and other components can be used for biodegradation of DDT present in contaminated soil and water ecosystems.

scholarly journals Isolation and characterization of Pseudomonas syringae isolates affecting stone fruits and almond in Montenegro

2021 ◽  
Author(s):  
Tamara Popović ◽  
Jelena Menković ◽  
Anđelka Prokić ◽  
Nevena Zlatković ◽  
Aleksa Obradović
Keyword(s):  
Pseudomonas Syringae ◽  
Housekeeping Genes ◽  
Molecular Characteristics ◽  
Biochemical Tests ◽  
High Genetic Diversity ◽  
Leaf Petiole ◽  
Molecular Features ◽  
Isolation And Characterization ◽  
Genomic Species

AbstractIn Montenegro, stone fruit species are grown on intensive and semi-intensive commercial plantations. However, almond production is mainly organized on family gardens and for household consumption. During two seasons (2017–2018), we surveyed apricot, peach, nectarine, sweet cherry, Japanese plum, and almond orchards for the presence of bacterial diseases at different geographical locations in Montenegro. From leaf, petiole and fruit lesions, branch or twig cankers, and necrotizing buds, a total of 29 isolates were obtained and subjected to identification based on their morphological, pathogenic, biochemical, and molecular characteristics. Pathogenicity of the isolates was confirmed by reproducing the symptoms on leaves, fruits, and twigs of the corresponding host plants. The biochemical tests indicated that the isolates belong to Pseudomonas syringae. However, isolates’ characterization showed variation in their phenotypic and molecular features. The presence of the syrB gene and ice nucleation activity grouped most of the isolates within pathovar syringae. The results of rep-PCR using the BOX primer revealed high genetic diversity of isolates. Multilocus sequence analysis (MLSA), using four housekeeping genes, showed that 27 isolates belong to the genomic species 1, P. syringae sensu stricto, corresponding to P. syringae phylogroup 2. However, isolates from the same phylogroup 2 did not form a monophyletic group. One strain isolated from apricot was most distinct and similar to members of genomic species 2, phylogroup 3. All tested isolates showed significant levels of resistance to copper sulfate and high level of sensitivity to streptomycin sulfate in vitro.

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