Development and validation of a novel customized medium for the marine microbial culture

Marine microbes are difficult to culture and hence it takes expensive measure to study. Mostly metagenomics are suggested for marine microbial characterization instead of culturing them in a suitable medium. In this study, a new approach has been developed to culture the marine microorganisms. We customized the LB agar and liquid medium by adjusting the pH and salt concentration at seawater level and then studied the microbial load, their growth rate by turbidity assay and also identified their morphology through gram staining. This study ensures the comfortable growth of marine microbes at the laboratory level, which would further help the marine biotechnologists and microbiologists to work smoothly with marine microbial samples. Bioresearch Commu. 7(2): 999-1003, 2021 (June)

Download Full-text

Development and validation of a combined temperature, water activity, pH model for bacterial growth rate of Lactobacillus curvatus

International Journal of Food Microbiology ◽

10.1016/s0168-1605(00)00401-3 ◽

2001 ◽

Vol 63 (1-2) ◽

pp. 57-64 ◽

Cited By ~ 62

Author(s):

T. Wijtzes ◽

F.M. Rombouts ◽

M.L.T. Kant-Muermans ◽

K. van ’t Riet ◽

M.H. Zwietering

Keyword(s):

Growth Rate ◽

Water Activity ◽

Bacterial Growth ◽

Lactobacillus Curvatus ◽

Bacterial Growth Rate ◽

Development And Validation ◽

Temperature Water

Download Full-text

Evaluating the Malaysian economy 2009-2018: growth, development and policies

10.32890/9789672363149 ◽

2020 ◽

Author(s):

Irwan Shah Zainal Abidin

Keyword(s):

Growth Rate ◽

Financial Crisis ◽

Prime Minister ◽

Global Financial Crisis ◽

High Growth ◽

Substantial Impact ◽

New Approach ◽

Asian Tigers ◽

New Vision ◽

National Transformation

Malaysia was once on the cusp of becoming one of the Asian Tigers as a result of the impressively high growth rates recorded in the early 1990s. From 1990 until 1997, the growth rate was above 9 percent per annum on average. This performance came to an end when the economy was struck by the 1997/98 Asian Financial Crisis, the worst economic crisis Malaysia has ever experienced since independence. Things eventually worsened with the onslaught of the 2008/09 Global Financial Crisis, which dragged the Malaysian economy yet into another round of a recession with the growth rate contracting at 1.5 percent in 2009. On hindsight, these two events, which have had a substantial impact on the state of the Malaysian economy, pointed to several urgent calls for economic reforms, such as the need to address structural weaknesses of the economy and to have a growth target which is both sustainable as well as inclusive. When Datuk Seri Najib Razak became the sixth Prime Minister of Malaysia from April 2009 until May 2018, it was clear that a new approach to economic development for Malaysia had to be crafted. Towards this end, he introduced the National Transformation Policy (NTP), so that the economy can be transformed into one that is of high-income and developed status by the year 2020. He also set a new vision for Malaysia, also known as the 2050 National Transformation, or TN50, which is meant to chart a new course for Malaysia to move into the second half of the 21st century. How successful is this transformational agenda? What are the other issues and challenges which need to be addressed? What important lessons can we learn from this transformational journey? This book is an attempt to address these specific questions by assessing Najibs economic plans, policies, programmes and vision which evolved during the nine years of his term as the sixth Prime Minister of Malaysia.

Download Full-text

Development and Validation of Load-Interaction Based Models for Crack Growth Prediction

Volume 2: Pipeline Integrity Management ◽

10.1115/ipc2014-33325 ◽

2014 ◽

Cited By ~ 3

Author(s):

Jiaxi Zhao ◽

Weixing Chen ◽

Sean Keane ◽

Jenny Been ◽

Greg Van Boven

Keyword(s):

Growth Rate ◽

Crack Growth Rate ◽

Crack Growth ◽

Life Prediction ◽

Pressure Fluctuations ◽

Remaining Life ◽

The Past ◽

Oil Pipelines ◽

Rainflow Counting ◽

Development And Validation

This investigation primarily focused on the validation of the software being developed for crack growth and remaining life prediction using SCADA data. A total of nine pressure spectra, four for oil pipelines and five for gas pipelines, have been collected and used as inputs for the software. It was found that these spectra could be categorized as the underload-, the meanload- and the overload-dominant spectra; each of them have shown different effects on crack growth: the underload spectra, typical of pressure fluctuations at the discharging sites, are most susceptible to crack growth because of load interactions between the minor pressure fluctuations and the unload cycles; while the overload spectra, often found at the suction site, have exhibited retarded crack growth due to the retardation effects caused by overloading. The relative severity of the load interactions in terms of crack growth rate for a given spectrum was quantified using a parameter termed as the Spectrum Factor. A Spectrum Factor greater than one indicates the enhanced crack growth rate by load interactions, such as the case where unloading is frequently present in the pressure spectra, while a Spectrum Factor lower than one may be associated with a retarded crack growth, which can be seen in pressure spectra with predominant overloading events. The predictions made by the models being developed were also compared with those made by the rainflow counting method. The software allows for the SCADA/pressure fluctuation data, in excel spreadsheet format, to be directly analyzed producing a projected remaining life of the pipeline based on the past pressure fluctuations and the assumed future pressure fluctuations.

Download Full-text

A New Approach: Software Development And Validation

Biomedical Instrumentation & Technology ◽

10.2345/0899-8205-45.5.373 ◽

2011 ◽

Vol 45 (5) ◽

pp. 373-377

Author(s):

Zhangwu Zhu

Keyword(s):

Software Development ◽

New Approach ◽

Development And Validation

Download Full-text

ISOLATION AND CHARACTERIZATION OF BIOTECHNOLOGY RELEVANT BACTERIA FROM MARINE ENVIRONMENT

Jurnal Teknologi ◽

10.11113/jt.v77.6893 ◽

2015 ◽

Vol 77 (31) ◽

Author(s):

Suganthi Thevarajoo ◽

Chitra Selvaratnam ◽

Kian Mau Goh ◽

Fazilah Abd. Manan ◽

Zaharah Ibrahim ◽

...

Keyword(s):

16S Rrna ◽

Marine Environment ◽

Antibiotic Sensitivity ◽

Marine Microorganisms ◽

Bacterial Strains ◽

Biochemical Tests ◽

16S Rrna Sequence ◽

Gram Staining ◽

Isolation And Characterization

Marine environment remained as largely unexplored source for researchers to discover marine microorganisms with novel properties. This study aims to isolate marine bacteria from the seashore of Desaru, Malaysia. Totally, six bacterial strains were successfully obtained and were identified by complete 16S rRNA sequencing. The characterizations of bacterial strains were performed based on morphological tests, Gram-staining, biochemical tests, and antibiotic sensitivity. The 16S rRNA sequence of D-2, D-4, D-7, D-15, D-31, and D-33 revealed a high identity of 97 to 99% with taxa belong to genera of Pseudomonas, Marinomonas, Exiquobacterium, Micrococcus, Pseudoalteromonas, and Shewanella respectively. Strain D-31 exhibited higher tolerance towards antibiotics kanamycin, ampicillin, and erythromycin while the growth of other strains were retarded by at least two of these antibiotics. We further characterized strain D-4 and D-31 that belonged to Marinomonas sp. and Pseudoalteromonas sp.. Both genera are interesting as earlier researchers have discovered new antibacterial substances, industrial enzymes and unique secondary metabolites.

Download Full-text