scholarly journals A Comparative Study between Helicobacter Pylori Antigen in Stool with Histopathology and Rapid Urease Test of Endoscopic Biopsy in PUD Patient

2021 ◽  
Vol 9 (2) ◽  
pp. 19-25
Author(s):  
Md Nazrul Islam Chowdhury ◽  
Syeed Mehbub Ul Kadir ◽  
Qamrun Naher ◽  
Lokesh Chakraborty ◽  
Mohammad Shahadat Hossain ◽  
...  
Keyword(s):  
Diagnostic Tool ◽  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Antigen Test ◽  
Cross Sectional ◽  
Stool Antigen Test ◽  
Ulcer Disease ◽  
Urease Test ◽  
Stool Antigen ◽  
H Pylori

Purpose: To assess stool antigen test as an early effective diagnostic tool like other methods for the diagnosis of H. pylori infection in PUD patient. Patients and Methods: This cross-sectional study was carried in a clinical pathology department of a tertiary level hospital. We included all patients with peptic ulcer disease those who were diagnosed by upper GIT endoscopy. Demographic variables and value of laboratory test including Stool antigen test, rapid urease test was studied in this study. Result: A total of eighty-six patients were undergone for endoscopy after tested for Helicobactor pylori antigen in stool. Among 86 study subjects, Male was 56 (65%) and female was 30 (35%). The Mean (±SD) age was found 38.53(±10.40) years with range from 21 to above 60 years. Stool antigen test positive in 66 (66/86, 76.74%), negative in 20 (20/86, 23.3%). Rapid Urease Test positive in 76 (76/86, 88.4%), negative in 20 (20/86, 11.6%). Histopathology positive in 65 (65/86, 75.58%), negative in 21 (21/86, 24.42%). The result was statistically highly significant (P<0.001). Conclusion: Stool antigen test (SAT) is an early effective diagnostic tool like other methods for the diagnosis of H. pylori infection in our country. CBMJ 2020 July: Vol. 09 No. 02 P: 19-25

scholarly journals Helicobacter pylori CagA seropositivity in adult Bangladeshi patients with peptic ulcer and erosion

2020 ◽  
Vol 14 (1) ◽  
pp. 36-40
Author(s):  
Fahmida Rahman ◽  
Khandaker Shadia ◽  
Salma Khatun ◽  
Mafruha Mahmud ◽  
Indrajit Kumar Dutta ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Ulcer Disease ◽  
Urease Test ◽  
Urease Production ◽  
Stool Antigen ◽  
H Pylori

Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. Methods: Any case that had peptic ulcer/erosion, plus positive for rapid urease test (RUT) or H. pylori stool antigen (HpSAg) or serum anti-H. pylori IgG/IgA were included in the study and named as H. pylori positive case. H. pylori positive cases were tested for CagA IgG antibody. Anti-H. pylori IgG, IgA and CagA IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and stool antigen by rapid immunochromatographic test (ICT). Urease production in biopsy sample was detected by RUT. Results: Total 86 H. pylori positive patients were included in the study. Out of 86 patients, CagA IgG was positive in 34 (39.5%; 95% CI: 0.30,0.50) cases. CagA seropositivity rate in ulcer and erosion cases were 58.8% (95% CI: 0.36,0.78) and 34.8% (95% CI: 0.25,0.47) respectively. H. pylori stool antigen and IgA antibodies were positive in all (100%) CagA antibody positive ulcer cases while the rates were significantly less among the CagA antibody negative cases (42.8% and 28.6%; p<0.05). However, in CagA antibody positive erosion cases, the rates were not significantly different from CagA antibody negative cases. Conclusion: The study has demonstrated that the CagA positive strain is less prevalent in erosion than ulcer cases. Ibrahim Med. Coll. J. 2020; 14(1): 36-40

scholarly journals Prevalence and factors associated with Helicobacter pylori infection among treatment naïve dyspeptic adults in University of Benin Teaching Hospital, Benin City, Nigeria

2020 ◽  
Vol 21 (2) ◽  
pp. 97-105
Author(s):  
A.O. Odigie ◽  
A.J. Adewole ◽  
A.A. Ekunwe
Keyword(s):  
Helicobacter Pylori ◽  
Teaching Hospital ◽  
Antigen Test ◽  
Cross Sectional ◽  
Stool Antigen Test ◽  
Factors Associated ◽  
Benin City ◽  
Treatment Naïve ◽  
Stool Antigen ◽  
H Pylori

Background: Infectious diseases such as malaria, HIV/AIDS and  tuberculosis have received tremendous attention globally but inspite of the widespread nature of infections caused by Helicobacter pylori, little attention has been paid to it especially in the developing countries. The objective of this study was to determine the prevalence of and factors associated with H. pylori infection among dyspeptic patients attending the University of Benin Teaching Hospital, Benin City, Nigeria. Methodology: This was a hospital based descriptive cross-sectional study of 354 treatment naive dyspeptic patients aged 18 to 44 years, recruited consecutively after obtaining institutional ethical approval and subjects’ informed consent. A pre-tested interviewer administered questionnaire was used to obtain subjects’ data. The stool antigen test was used to detect H. pylori infection. Results: The prevalence of H. pylori infection among the subject  participants was 34.2% (38.4% in female, 24.0% in male, p=0.009). The age group < 20 years had the highest prevalence of 40%. Gender,  occupation, increased body mass index, high number of household  occupants, and rural dwelling, were significantly associated with H. pylori infection (p<0.05) and alcohol intake was inversely related to H. pylori infection (p<0.05). Conclusion: The prevalence of H. pylori infection in University of Benin Teaching Hospital is high and factors such as gender, obesity, occupation with risk of contact, low socio-economic status, and poor hygiene, may be responsible for this.Keywords: Dyspepsia, Helicobacter pylori, infection, stool antigen test

scholarly journals Assessment of a novel method to detect clarithromycin-resistant Helicobacter pylori using a stool antigen test reagent

2020 ◽  
Author(s):  
Toshihiko Kakiuchi ◽  
Kazutoshi Hashiguchi ◽  
Ichiro Imamura ◽  
Aiko Nakayama ◽  
Ayako Takamori ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Nested Pcr ◽  
Invasive Technique ◽  
Antigen Test ◽  
Resistance Mutations ◽  
Stool Antigen Test ◽  
Urease Test ◽  
Stool Antigen ◽  
Novel Method ◽  
H Pylori

Abstract Background The resistance rate of Helicobacter pylori to clarithromycin (CAM) is high among the infected children in Japan; therefore, a new method for detecting CAM-resistant H. pylori using a less invasive technique is strongly desired. We aimed to confirm the clinical usefulness of our newly developed Nested polymerase chain reaction-QProbe (quenching probe) (Nested PCR-QP) method using stool specimens. Methods The first was an evaluation of our method using the residual solution of the H. pylori stool antigen test for adolescents, and the second was an evaluation of our method using culture testing for adults. Results Of the 57 middle school students with H. pylori , the Nested PCR-QP test results of 53 (90.3%) could be analyzed; 28 students were found to have CAM resistance mutations. The results indicating genetic mutation in 28 and no mutation in 23 students were consistent with those of PCR-direct sequencing. In the 23 adults who were diagnosed with H. pylori infection using the rapid urease test and culture testing, it was possible to use Nested PCR-QP for analyzing all 21 adults who tested positive in the stool H. pylori antigen test. The results obtained for all the 21 adults were consistent with those obtained via the drug susceptibility test. Conclusions Our novel method could be useful for non-invasively detecting CAM resistance mutations in H. pylori. This may help select an eradication drug to reduce eradication failure rates against H. pylori.

scholarly journals Assessment of a novel method to detect clarithromycin-resistant Helicobacter pylori using a stool antigen test reagent

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Toshihiko Kakiuchi ◽  
Kazutoshi Hashiguchi ◽  
Ichiro Imamura ◽  
Aiko Nakayama ◽  
Ayako Takamori ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Nested Pcr ◽  
Invasive Technique ◽  
Antigen Test ◽  
Resistance Mutations ◽  
Stool Antigen Test ◽  
Urease Test ◽  
Stool Antigen ◽  
Novel Method ◽  
H Pylori

Abstract Background The resistance rate of Helicobacter pylori to clarithromycin (CAM) is high among infected children in Japan. Therefore, a new method for detecting CAM-resistant H. pylori using a minimally invasive technique is strongly desired. We aimed to investigate the clinical usefulness of our newly developed nested polymerase chain reaction-quenching probe (Nested PCR-QP) method using stool specimens. Methods We first evaluated our method using a residual solution of the H. pylori stool antigen test for adolescents. Then, we evaluated our method using culture testing for adults. Results Among 57 middle school students with H. pylori, the Nested PCR-QP test results of 53 (90.3%) were able to be analyzed. A total of 28 students had CAM resistance mutations. We found a genetic mutation in 28 students and no mutation in 23 students, and these results were consistent with those of PCR-direct sequencing. In the 23 adults who were diagnosed with H. pylori infection using the rapid urease test and culture testing, we were able to use Nested PCR-QP for analyzing 21 adults who tested positive in the stool H. pylori antigen test. The results obtained for all 21 adults were consistent with those obtained via the drug susceptibility test. Conclusions Our novel method could be useful for non-invasively detecting CAM resistance mutations in H. pylori. This may help select a drug to reduce eradication failure rates against H. pylori. Trial registration This study was registered with the University Hospital Medical Information Network Clinical Trials Registry (no. UMIN000030632, https://upload.umin.ac.jp/cgi-open-bin/ctr_e/ctr_view.cgi?recptno=R000034977) on 29 December 2017.

scholarly journals Does spontaneous eradication of Helicobacter pylori infection lead to functional dyspepsia? 

2021 ◽  
Author(s):  
Shahram Habibzadeh ◽  
Mohammad Reza Aslani ◽  
Abbas Yazdanbod ◽  
Monouchehr Iranparvar Alamdari ◽  
Babak Chakarabbaci ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Functional Dyspepsia ◽  
Specific Treatment ◽  
Rapid Urease Test ◽  
Cross Sectional ◽  
Stool Antigen Test ◽  
Urease Test ◽  
Significant Difference ◽  
History Of ◽  
H Pylori

Abstract Introduction: The treatment of patients with functional dyspepsia in cases of active helicobacter pylori (H. pylori) infection is not promising. Therefore, the present study was designed to determine in which of the dyspeptic patients with the normal endoscopic examination and negative stool testing for H. pylori antigen (without a history of specific treatment for H. pylori) there is evidence of a previous H. pylori infection in the serum.Materials and methods: In this cross-sectional study, patients with functional dyspepsia who were negative in terms of the H. pylori stool antigen test and rapid urease test, and had no obvious gross pathologic sign in gastroduodenoscopy were considered suitable candidates for serological study for the detection of H. pylori IgG specific antibodies.Results: A total of 200 patients were enrolled in this study, including 86 men (43%) and 114 women (57%), with an average age of 38.76 ± 12.35 years. The results showed that 109 (54.5%) were positive subjects were positive by anti-H. pylori IgG ELISA tests. It was found that positive anti-H. pylori IgG ELISA tests were a higher significant difference among rural residents (75.2%) compared with urban residents (24%) (p<0.001).Conclusion: More than half of patients with functional dyspepsia have a history of previous H.pylori infection. Despite the possibility of spontaneous H. pylori infection, the complication of old infections may be sustained by dyspepsia and early treatment of acute H. pylori infections may prevent this complication.

scholarly journals Noninvasive diagnosis of Helicobacter pylori among patients with dyspepsia

2020 ◽  
Vol 5 (1) ◽  
pp. 28-34
Author(s):  
Suresh Thapa ◽  
Jiwan Thapa ◽  
Binod Karki ◽  
Dibas Khadka ◽  
Anju Bhandari Thapa
Keyword(s):  
Helicobacter Pylori ◽  
Common Disease ◽  
Antigen Test ◽  
Cross Sectional ◽  
Stool Antigen Test ◽  
Resource Limited ◽  
Gastric Biopsies ◽  
Stool Antigen ◽  
H Pylori ◽  
Good Diagnostic Accuracy

Introduction: Helicobacter pylori (H. pylori) infection is a common disease with disparity in geography, age, race and socioeconomic status. Present study aims to assess the accuracy of noninvasive diagnostic tests. Methods: This is a hospital based cross sectional, observational study of 100 dyspeptic patients during November 2017- June 2018. Profile of dyspeptic patients were recorded in a proforma and accuracy of serology and stool antigen test for H. pylori was compared with histology detection in gastric biopsies. Results: Mean age of patients was 39.5±13.7 years with almost equal gender distribution. Prevalence of H. pylori was 56% with dyspeptic symptoms in gastric biopsies. Significant association of the infection was seen in patients with a longer duration of dyspepsia, pangastritis on endoscopy and chronic gastritis on histologic examination. Stool antigen test had good sensitivity (71%) and specificity (82%) for H. pylori detection compared with standard histological diagnosis. Conclusion: Overall prevalence of H. pylori was 56% and was more common in patients of age > 44 years with dyspepsia. Stool antigen has a good diagnostic accuracy and can be used in resource limited settings. However, we recommend a community based study in larger population.

scholarly journals PCR-Based Detection and Genotyping ofHelicobacter pyloriin Endoscopic Biopsy Samples from Brazilian Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Silvia M. Ferreira Menoni ◽  
Sandra Helena Alves Bonon ◽  
José Murilo Robilota Zeitune ◽  
Sandra Cecília Botelho Costa
Keyword(s):  
Gastrointestinal Symptoms ◽  
Restriction Enzymes ◽  
Endoscopic Biopsy ◽  
Future Research ◽  
Rapid Urease Test ◽  
Computational Program ◽  
Urease Test ◽  
Precise Diagnosis ◽  
H Pylori ◽  
Molecular Biology Method

Helicobacter pylori(H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests. PCR was used in the detection and genotyping of theH. pyloriurease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive forH. pyloriaccording to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive forH. pyloriaccording to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positiveH. pylorisamples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzymeHhaIand 12 patterns with the enzymeMboIwere found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.

scholarly journals Urine-Based ELISA for the Detection of Helicobacter pylori IgG Antibody and Comparison with Other Invasive Methods.

1970 ◽  
Vol 4 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Md Din-ul Islam ◽  
Sufi HZ Rahman ◽  
SM Shamsuzzaman ◽  
Naima Muazzaman ◽  
Nasim Ahmed ◽  
...  
Keyword(s):  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Medical College ◽  
Urease Test ◽  
Study Population ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Upper Gastrointestinal

The present study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka during the period of January, 2007 to December, 2007. Urine samples were collected from 86 dyspeptic patients undergoing upper Gastrointestinal Tract (GIT) endoscopy to determine anti-H. pylori IgG antibody by an ELISA method. Gastric biopsy tissues were tested for culture, rapid urease test and H&E/Giemsa stain. Out of 86 endoscopic biopsy specimens, 45 (52.33%) were culture positive, 63 (73.26%) were rapid urease test positive and 64 (74.42%) were H&E/Giemsa stained positive for H. pylori. According to operational standard definition, among the 86 study population, 66 (76.74%) were H. pylori infected, 16 (18.60%) were uninfected and 4 (4.65%) were indeterminate. Among 66 H. pylori infected cases, 63 (95.45%) were urine ELISA positive and among 16 uninfected cases 3 (18.75%) were urine ELISA positive. Out of 86 study population, 66 (76.74%) were urine ELISA positive. The sensitivity, specificity, PPV, NPP and accuracy of urine ELISA were 95.45%, 81.25%, 95.45%, 81.25% and 92.68% respectively. The result of the study shows that H. pylori infection can be rapidly and reliably diagnosed by detecting anti-H. pylori IgG from urine. Key words: H. pylori; ELISA; GIT; IgG. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8463 BJMM 2011; 4(1): 14-17

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