scholarly journals Urine-Based ELISA for the Detection of Helicobacter pylori IgG Antibody and Comparison with Other Invasive Methods.

1970 ◽  
Vol 4 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Md Din-ul Islam ◽  
Sufi HZ Rahman ◽  
SM Shamsuzzaman ◽  
Naima Muazzaman ◽  
Nasim Ahmed ◽  
...  
Keyword(s):  
Endoscopic Biopsy ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Medical College ◽  
Urease Test ◽  
Study Population ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Culture Positive ◽  
Upper Gastrointestinal

The present study was conducted in the department of Microbiology, Dhaka Medical College, Dhaka during the period of January, 2007 to December, 2007. Urine samples were collected from 86 dyspeptic patients undergoing upper Gastrointestinal Tract (GIT) endoscopy to determine anti-H. pylori IgG antibody by an ELISA method. Gastric biopsy tissues were tested for culture, rapid urease test and H&E/Giemsa stain. Out of 86 endoscopic biopsy specimens, 45 (52.33%) were culture positive, 63 (73.26%) were rapid urease test positive and 64 (74.42%) were H&E/Giemsa stained positive for H. pylori. According to operational standard definition, among the 86 study population, 66 (76.74%) were H. pylori infected, 16 (18.60%) were uninfected and 4 (4.65%) were indeterminate. Among 66 H. pylori infected cases, 63 (95.45%) were urine ELISA positive and among 16 uninfected cases 3 (18.75%) were urine ELISA positive. Out of 86 study population, 66 (76.74%) were urine ELISA positive. The sensitivity, specificity, PPV, NPP and accuracy of urine ELISA were 95.45%, 81.25%, 95.45%, 81.25% and 92.68% respectively. The result of the study shows that H. pylori infection can be rapidly and reliably diagnosed by detecting anti-H. pylori IgG from urine. Key words: H. pylori; ELISA; GIT; IgG. DOI: http://dx.doi.org/10.3329/bjmm.v4i1.8463 BJMM 2011; 4(1): 14-17

scholarly journals Performance of RoutineHelicobacter pyloriInvasive Tests in Patients with Dyspepsia

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Hsi-Chang Lee ◽  
Ting-Chang Huang ◽  
Chin-Lin Lin ◽  
Kuan-Yang Chen ◽  
Chung-Kwe Wang ◽  
...  
Keyword(s):  
Predictive Value ◽  
Gastrointestinal Endoscopy ◽  
Positive Culture ◽  
Upper Gastrointestinal Endoscopy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Upper Gastrointestinal ◽  
Antral Biopsy

Background. This study was designed to compare the accuracy of three different invasive methods for the detection ofHelicobacter pylori (H. pylori)infection in patients with dyspepsia. These tests included culture, histology, and the rapid urease test (CLO test).Methods.H. pyloriinfection was diagnosed prospectively in 246 untreated dyspeptic patients who underwent upper gastrointestinal endoscopy. The gold standard forH. pyloriinfection was based on a positive culture or both a positive histological examination and a CLO test.Results.H. pyloriwas diagnosed in 33.3% of the patients. The sensitivity, specificity, positive predictive value, negative predictive value, and overall accuracy were as follows: histology from the antrum (95.12; 95.12; 90.7; 97.5; 95.12%); histology from the antrum and corpus (95.12; 95.12; 90.7; 97.5; 95.12%); histology from the corpus (76.83; 96.95; 92.65; 89.33; 90.24%); culture (91.46; 100; 100; 95.91; 97.15%); a CLO test from the antrum and corpus (85.59; 100; 100; 93.71; 95.52%); a CLO test from the antrum (64.63; 100; 100; 84.97; 88.21%); a CLO test from the corpus (69.51; 100; 100; 96.77; 89.83%), respectively.Conclusions. Antral biopsy histology and culture are the best methods for the diagnosis ofH. pyloriinfection in our cohort of patients with dyspepsia.

Helicobacter pylori infection in dyspeptic patients in an industrial belt of India

2016 ◽  
Vol 47 (1) ◽  
pp. 2-6 ◽  
Author(s):  
Parthasarathi Satpathi ◽  
Sanghamitra Satpathi ◽  
Sanjib Mohanty ◽  
Saroj K Mishra ◽  
Prativa K Behera ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Endoscopic Biopsy ◽  
Gastric Biopsy ◽  
Gram Stain ◽  
Chronic Active Gastritis ◽  
Gastrointestinal Pathology ◽  
Urease Test ◽  
H Pylori ◽  
Sensitivity Specificity ◽  
Upper Gastrointestinal

The present study is done to study different aspects of Helicobacter pylori ( H. pylori) such as its prevalence, association with upper gastrointestinal pathology, diagnosis and treatment outcome. Gastric antral biopsy and serology for H. pylori was done for all dyspeptic patients. Histopathology, gram stain and biopsy urease test was done from the gastric biopsy specimen. The prevalence of H. pylori infection was 58.8%. The sensitivity, specificity, positive and negative predictive value for histopathology was 96.9%, 100%, 100% and 95.8%, respectively; for biopsy urease test 80.4%, 100%, 100% and 78.2%, respectively; for gram stain 85.6%, 97.1%, 97.6% and 82.5%, respectively, and for serology 94.8%, 77.9%, 86% and 91.4%, respectively. Mostly peptic ulcer and duodenitis cases followed by chronic active gastritis were associated with H. pylori infection. Repeat biopsy revealed eradication of H. pylori in 90.7% cases. In dyspeptic patients, endoscopic biopsy not only detects H. pylori infection, but also reveals different gastric pathologies.

Good diagnostic accuracy of a chemiluminescent immunoassay in stool samples for diagnosis of Helicobacter pylori infection in patients with dyspepsia

2016 ◽  
Vol 64 (2) ◽  
pp. 388-391 ◽  
Author(s):  
María José Ramírez-Lázaro ◽  
Josep Lite ◽  
Sergio Lario ◽  
Pepa Pérez-Jové ◽  
Antònia Montserrat ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gold Standard ◽  
Rapid Urease Test ◽  
Diagnostic Reliability ◽  
Predictive Values ◽  
Urease Test ◽  
Stool Samples ◽  
H Pylori ◽  
Good Diagnostic Accuracy ◽  
Sensitivity Specificity

Laboratory-based chemiluminescence immunoassays (CLIA) are widely used in clinical laboratories. Some years ago, a CLIA test was developed for the detection of Helicobacter pylori in stool samples, known as LIAISON H. pylori SA, but little information on its use has been reported. To evaluate the accuracy of the LIAISON H. pylori SA assay for diagnosing H. pylori infection prior to eradication treatment. Diagnostic reliability was evaluated in 252 untreated consecutive patients with dyspepsia. The gold standard for diagnosing H. pylori infection was defined as the concordance of the rapid urease test (RUT), histopathology and urea breath test (UBT). The CLIA assay was performed according to the manufacturer's instructions. Sensitivity, specificity, positive and negative predictive values, and 95% CIs were calculated. According to the gold standard selected, 121 patients were positive for H. pylori infection and 131 negative. LIAISON H. pylori SA had a sensitivity of 90.1% and a specificity of 92.4%, with positive and negative predictive values of 91.6% and 90.1%, respectively. The accuracy of the LIAISON H. pylori SA chemiluminescent diagnostic assay seems comparable to that of ELISA or the best-performing LFIAs. Its sensitivity and specificity, however, seem slightly lower than those of histology, RUT or UBT. The advantages of the assay are that it is cheap, automated, and minimally labor-intensive.

scholarly journals PCR-Based Detection and Genotyping ofHelicobacter pyloriin Endoscopic Biopsy Samples from Brazilian Patients

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Silvia M. Ferreira Menoni ◽  
Sandra Helena Alves Bonon ◽  
José Murilo Robilota Zeitune ◽  
Sandra Cecília Botelho Costa
Keyword(s):  
Gastrointestinal Symptoms ◽  
Restriction Enzymes ◽  
Endoscopic Biopsy ◽  
Future Research ◽  
Rapid Urease Test ◽  
Computational Program ◽  
Urease Test ◽  
Precise Diagnosis ◽  
H Pylori ◽  
Molecular Biology Method

Helicobacter pylori(H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests. PCR was used in the detection and genotyping of theH. pyloriurease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive forH. pyloriaccording to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive forH. pyloriaccording to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positiveH. pylorisamples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzymeHhaIand 12 patterns with the enzymeMboIwere found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.

scholarly journals Helicobacter pylori culture as a key tool for diagnosis in Colombia

2019 ◽  
Vol 13 (08) ◽  
pp. 720-726
Author(s):  
Diana F Rojas-Rengifo ◽  
Belen Mendoza ◽  
Carlos Jaramillo ◽  
Paula A Rodríguez-Urrego ◽  
José F Vera-Chamorro ◽  
...  
Keyword(s):  
Predictive Value ◽  
Optimal Growth ◽  
Rapid Urease Test ◽  
Culture Methods ◽  
Essential Information ◽  
Urease Enzyme ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori ◽  
Sensitivity Specificity

Introduction: The presence of H. pylori in the stomach is associated with gastric pathologies. However, its diagnosis through culture methods is challenging because of its complex nutritional requirements and microaerophilic conditions for optimal growth. The preferred method for rapid diagnosis of H. pylori is the Rapid Urease Test (RUT) from human biopsies, which relies on the high activity of the urease enzyme present in H. pylori. However, RUT cannot say much more information about H. pylori. This makes evident the need for bacterial culture to know essential information such as the strain type, the kind of infection present and the bacteria’s antibiotic susceptibility. Methodology: Gastric biopsies from 347 patients were used for H. pylori isolation. We correlated the culture results with the RUT and histological grading used at Hospital Universitario Fundación SantaFe de Bogotá (HU-FSFB), Colombia. The concordance between techniques was determined by the Cohen’s Kappa coefficient (K). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were also calculated. Results: The culture standardization was successful, and it could be applied for diagnosis in the clinical practice. H. pylori was positive by culture in 88 (26.34%) patients. The concordance of RUT and culture was strong (K= 0.805), and between histology and culture was moderate (K= 0.763) as well as for the gold standard defined and culture (K= 0.80). Conclusions: We present evidence that RUT and histological methods will be better interpreted for diagnosis of H. pylori if combined with bacterial isolation in cholesterol enriched culture.

scholarly journals Diagnostic accuracy of “sweeping” method compared to conventional sampling in rapid urease test for Helicobacter pylori detection in atrophic mucosa

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Choong-Kyun Noh ◽  
Gil Ho Lee ◽  
Jin Woong Park ◽  
Jin Roh ◽  
Jae Ho Han ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Conventional Method ◽  
Receiver Operating Curve ◽  
Rapid Urease Test ◽  
Detection Time ◽  
Simple Method ◽  
Urease Test ◽  
Sweeping Method ◽  
H Pylori ◽  
Sensitivity Specificity

Abstract Although the rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. We compared the diagnostic performance of a “sweeping method” for H. pylori detection with the conventional biopsy sampling method in atrophic gastric conditions which can reduce RUT accuracy. This prospective study included 279 patients who underwent upper endoscopy to determine the presence of H. pylori infection. Gastric mucosa of both the antrum and the corpus were swabbed, and we named this method the “sweeping method”. Biopsy sampling for the conventional method, histologic evaluation, and polymerase chain reaction were performed at the same time. The sensitivity, specificity, and accuracy of the sweeping method were 0.941, 0.826, and 0.903, respectively, compared to 0.685, 0.859, and 0.742, respectively, for the conventional biopsy method. The area under the receiver operating curve for the sweeping method was 0.884 versus 0.772 for the conventional method (P < 0.001). The sweeping method had a faster detection time than the conventional method. Compared to conventional biopsy sampling, the sweeping method with the RUT provided higher sensitivity and accuracy for the detection of H. pylori, with a faster detection time.

scholarly journals Prevalence of Helicobacter pylori in patients with peptic ulcer disease

2018 ◽  
Vol 5 (4) ◽  
pp. 1315
Author(s):  
Mrutyunjay I. Uppin ◽  
Kapildev K. Hannurkar
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Peptic Ulcer Disease ◽  
Histopathological Examination ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Ulcer Disease ◽  
Urease Test ◽  
H Pylori ◽  
Upper Gastrointestinal

Background: Prevalence of Helicobacter pylori (H. pylori) emerges throughout the world and instigates peptic ulcer disease (PUD). The study was conducted with the aim to determine the prevalence of H. pylori in patients with PUD undergoing upper gastrointestinal endoscopy.Methods: This prospective study was conducted on 150 cases of PUD from August 2009 to February 2011. Endoscopy was done in all cases. Biopsy was done and sent for histopathological examination and rapid urease test for confirmation of presence of H. pylori.Results: Out of 150 patients with mean age of 45.76 years, 109 patients were diagnosed to have been infected with Helicobacter pylori (72.66%). Out of 89 patients with gastric ulcer, 61 patients were infected with Helicobacter pylori (68.53%). Forty two out of 51 patients (82.35%) with duodenal ulcers and 06 of 10 patients (60%) with carcinoma of stomach were positive for H. pylori. The remaining patients were found to be negative for the H. pylori infection.Conclusions: The findings of the study conclude that H. pylori was consistently associated with PUD.

scholarly journals Helicobacter pylori CagA seropositivity in adult Bangladeshi patients with peptic ulcer and erosion

2020 ◽  
Vol 14 (1) ◽  
pp. 36-40
Author(s):  
Fahmida Rahman ◽  
Khandaker Shadia ◽  
Salma Khatun ◽  
Mafruha Mahmud ◽  
Indrajit Kumar Dutta ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Peptic Ulcer ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rapid Urease Test ◽  
Igg Antibody ◽  
Ulcer Disease ◽  
Urease Test ◽  
Urease Production ◽  
Stool Antigen ◽  
H Pylori

Background: CagA IgG antibody in sera might indicate presence of virulent Helicobacter pylori in patients with peptic ulcer disease. Present study was performed to find out the prevalence of CagA IgG antibody in patients with peptic ulcer/erosion. Methods: Any case that had peptic ulcer/erosion, plus positive for rapid urease test (RUT) or H. pylori stool antigen (HpSAg) or serum anti-H. pylori IgG/IgA were included in the study and named as H. pylori positive case. H. pylori positive cases were tested for CagA IgG antibody. Anti-H. pylori IgG, IgA and CagA IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA) and stool antigen by rapid immunochromatographic test (ICT). Urease production in biopsy sample was detected by RUT. Results: Total 86 H. pylori positive patients were included in the study. Out of 86 patients, CagA IgG was positive in 34 (39.5%; 95% CI: 0.30,0.50) cases. CagA seropositivity rate in ulcer and erosion cases were 58.8% (95% CI: 0.36,0.78) and 34.8% (95% CI: 0.25,0.47) respectively. H. pylori stool antigen and IgA antibodies were positive in all (100%) CagA antibody positive ulcer cases while the rates were significantly less among the CagA antibody negative cases (42.8% and 28.6%; p<0.05). However, in CagA antibody positive erosion cases, the rates were not significantly different from CagA antibody negative cases. Conclusion: The study has demonstrated that the CagA positive strain is less prevalent in erosion than ulcer cases. Ibrahim Med. Coll. J. 2020; 14(1): 36-40

scholarly journals Comparative study of rapid urease test and histopathological examination for detection of H. pylori infection

2017 ◽  
Vol 4 (12) ◽  
pp. 4071
Author(s):  
Virendra S. Athavale ◽  
Vinit Singh ◽  
Shivmurti N. Khandalkar ◽  
Dakshayani S. Nirhale ◽  
Aditya Lad ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Good Alternative ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Study Group ◽  
Medical College ◽  
Urease Test ◽  
Resource Poor ◽  
H Pylori

Background: Aims and objectives of the study was to study the incidence of H. pylori infection in our Hospital. To find out the sensitivity and specificity of rapid urease test (RUT) and histopathological examination for the detection of H. pylori on gastric biopsy. To study the effect of anti H. pylori drugs by performing follow-up endoscopy in terms of positive or negative Rapid Urease Test and Histopathological Examination report.Methods: The study was conducted at Dr. D. Y. Patil Medical College and Hospital, DPU University, for a period of 2 years (from July 2012-September 2014) and is a prospective and comparative randomized type of study using 100 patients. The study was approved by the Institute’s Ethics Committee.Results: Data analysis showed that: In our study 84 patients (84%) were detected positive by RUT method. In our study 83 patients (83 %) were detected positive by HPE method. There was an association between RUT and HPE finding in study group with the sensitivity being 96.38 % and specificity being 74.47 %, PPV was 95.24% and NPV was 81.25%. At the time of follow up after taking anti H pylori treatment, 79 patients were RUT negative and 8 were RUT positive who were defaulters (not taken complete dose) and again started on treatment with the accuracy of the drug being 98.85 %. At the time of follow up after taking anti H pylori treatment 78 patients were HPE negative and 9 were HPE positive who were defaulters (not taken complete dose) and again started on treatment. There was an association between RUT and HPE finding at follow up with the sensitivity being 88.89 % and specificity being 100%, PPV was 100% and NPV was 98.73%.Conclusions: Our study reveals that RUT is accurate for the diagnosis of H. pylori infection and its use will serve as a good alternative to histology in management of patients with dyspepsia in resource poor environments, except in patients who need histology for reasons other than H. pylori diagnosis.

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