scholarly journals Marker-assisted Backcrossing for Identification of Salt Tolerant Rice Lines

2013 ◽  
Vol 2 (2) ◽  
pp. 1-8 ◽  
Author(s):  
M Moniruzzaman ◽  
MS Islam ◽  
JA Rashid ◽  
SN Begum ◽  
MM Islam
Keyword(s):  
Genetic Diversity ◽  
Salt Tolerance ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Genetic Maps ◽  
Salt Tolerant ◽  
High Yielding Variety ◽  
Marker Assisted Backcrossing ◽  
Tolerant Genotype ◽  
New Cultivars

SSR or microsatellite markers are proved to be ideal for making genetic maps, assisting selection and studying genetic diversity in germplasm. SSR markers are playing important role to identify gene for salt tolerance that can be helpful for plant breeders to develop new cultivars. The experiment was conducted during the period from July 2009 to November 2010 in the experimental field and Biotechnology Laboratory of Plant Breeding Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh to identify salt tolerant rice line of BC1F1 progenies of Binadhan-5 x FL-478 using SSR markers. Salt tolerant genotype, FL-478 was crossed with high yielding variety, Binadhan-5. Randomly selected 40 BC1F1 progenies along with their two parents (Binadhan-5, FL-478 and F1) were genotyped with microsatellite or SSR markers for identification of salt tolerant rice lines. Parental polymorphism survey was assayed by 10 SSR markers and three polymorphic SSR markers viz., RM 336, RM 510, and RM 585 were selected to evaluate BC1F1 rice lines for salt tolerance. In respect of Primer RM 336, 11 lines were found as salt tolerant and 25 lines were heterozygous and 3 lines were susceptible. Primer RM 510 identified two tolerant, 14 heterozygous and 22 susceptible lines. And primer RM 585 identified 4 lines as tolerant and 35 lines as susceptible. Thus, these markers could be efficiently used in tagging salt tolerant genes, in marker-assisted selection and quantitative trait loci (QTL) mapping. The selected BC1F1 could be used for developing BC2F1 and BC2F2 and mapping genes for salinity tolerance. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14008 Int. J. Agril. Res. Innov. & Tech. 2 (2): 1-8, December, 2012

Application of SSR Technique for the Identification of Markers Linked to Salinity Tolerance in Rice

2013 ◽  
Vol 19 (2) ◽  
pp. 57-65
Author(s):  
MH Kabir ◽  
MM Islam ◽  
SN Begum ◽  
AC Manidas
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Salinity Tolerance ◽  
Seedling Stage ◽  
Phenotypic Screening ◽  
Salt Tolerant ◽  
Marker Assisted Breeding ◽  
Hydroponic System ◽  
Rice Landrace ◽  
Salt Susceptible

A cross was made between high yielding salt susceptible BINA variety (Binadhan-5) with salt tolerant rice landrace (Harkuch) to identify salt tolerant rice lines. Thirty six F3 rice lines of Binadhan-5 x Harkuch were tested for salinity tolerance at the seedling stage in hydroponic system using nutrient solution. In F3 population, six lines were found as salt tolerant and 10 lines were moderately tolerant based on phenotypic screening at the seedling stage. Twelve SSR markers were used for parental survey and among them three polymorphic SSR markers viz., OSR34, RM443 and RM169 were selected to evaluate 26 F3 rice lines for salt tolerance. With respect to marker OSR34, 15 lines were identified as salt tolerant, 9 lines were susceptible and 2 lines were heterozygous. While RM443 identified 3 tolerant, 14 susceptible and 9 heterozygous rice lines. Eight tolerant, 11 susceptible and 7 heterozygous lines were identified with the marker RM169. Thus the tested markers could be efficiently used for tagging salt tolerant genes in marker-assisted breeding programme.DOI: http://dx.doi.org/10.3329/pa.v19i2.16929 Progress. Agric. 19(2): 57 - 65, 2008

Molecular breeding for combating salinity stress in sorghum: progress and prospects.

2021 ◽  
pp. 421-432
Author(s):  
Nidhi Chakma ◽  
Moutoshi Chakraborty ◽  
Salma Bhyan ◽  
Mobashwer Alam
Keyword(s):  
Salt Tolerance ◽  
Marker Assisted Selection ◽  
Molecular Breeding ◽  
Field Conditions ◽  
Salt Tolerant ◽  
Conventional Breeding ◽  
Qtl Validation ◽  
Marker Validation ◽  
Controlled Environments ◽  
Breeding Techniques

Abstract This chapter discusses current progress and prospects of molecular breeding and strategies for developing better saline-tolerant sorghum (Sorghum bicolor) varieties. Most molecular breeding techniques for salt tolerance have been carried out in controlled environments where the plants were not exposed to any variation of the surrounding environment, producing reliable results. Due to the polygenic nature of salt tolerance, the identified quantitative trait loci (QTLs) could be false QTLs. Therefore, QTL validation is important in different plant populations and field conditions. Subsequently, marker validation is important before utilizing marker-assisted selection for screening salt-tolerant plants. Combining molecular breeding with conventional breeding can hasten the development of salt-tolerant sorghum varieties.

scholarly journals Genetic Diversity Analysis of Some Barley Genotypes for Salt Tolerance Using SSR Markers

2013 ◽  
Vol 5 (7) ◽  
Author(s):  
Samah A. Mariey ◽  
Maher Noaman Mohamed ◽  
Ismail A. Khatab ◽  
Antar N. El-Banna ◽  
Amro Farouk Abdel Khalek ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Salt Tolerance ◽  
Ssr Markers ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
Barley Genotypes

scholarly journals The Development of the Genic SSR Markers for Analysis of Genetic Diversity in Gooseberry Cultivars

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1050
Author(s):  
Elena O. Vidyagina ◽  
Vadim G. Lebedev ◽  
Natalya M. Subbotina ◽  
Ella I. Treschevskaya ◽  
Tatyana N. Lebedeva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Marker Assisted Selection ◽  
Flavonoid Biosynthesis ◽  
Phenotypic Traits ◽  
Modern Approach ◽  
Genic Ssr ◽  
Small Fruit ◽  
Interspecies Hybrids ◽  
Different Origins

Gooseberry is one of the most promising and underrated small fruit crops. There is a need to improve and genotype the existing cultivars, and this work requires the use of new efficient DNA marking techniques. Marker-assisted selection (MAS) is a modern approach for work with genetic resources. However, the genetic determinants of key qualitative traits are poorly studied. Therefore, we developed SSR markers located in flavonoid biosynthesis genes that can affect the resistance of plants to biotic and abiotic stresses to study the genetic diversity of gooseberry cultivars grown in the Russian Federation and varying in genetic and geographical origin. We have genotyped 22 gooseberry cultivars using a set of 19 of our original microsatellite markers and two neutral previously published ones. A total of 53 alleles were found. Nine of the 21 analyzed loci were polymorphic. The most polymorphic loci of flavonoid biosynthesis were found in the DFR gene (RucDFR1-2, RucDFR1-3, RucDFR2-1), their mean PIC (polymorphism information content) being 0.65, comparable to the PIC values of neutral markers. Our set of gene-targeted SSR markers showed that most of all the studied gooseberry cultivars differed in origin, based on which they were divided into three groups: European cultivars, Russian cultivars, and interspecies hybrids. Thus, the polymorphic markers can be used for cultivar identification and certification as well as for the marker-assisted selection of gooseberry plants having different origins and phenotypic traits.

scholarly journals Identification of introgressed rice lines of Binadhan-7×FL-378 under salt stress through SSR markers

2012 ◽  
Vol 10 (1) ◽  
pp. 49-54 ◽  
Author(s):  
M A Haque ◽  
M A Mahmud ◽  
M M Islam ◽  
S N Begum
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Salt Tolerant ◽  
Bc1f1 Population ◽  
Backcross Population ◽  
Ssr Loci ◽  
Salt Susceptible ◽  
Pattern Of Variation ◽  
Positive Correlations

Thirty two rice lines of BC1F1 population (Binadhan-7/FL 378) were used to identify introgressed rice lines for salt tolerance using SSR markers at the Plant Breeding and Biotechnology Divisions of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Seeds of BC1F1 population of Binadhan-7/FL-378 were collected from backcross population of F1 (Binadhan-7/FL 378). Salt tolerant genotype, FL-378 was crossed with high yielding variety, Binadhan-7. Randomly selected 32 BC1F1 progenies along with their two parents (Binadhan-7, FL-378) were genotyped with microsatellite or SSR markers for identification of introgressed rice lines. Parental polymorphism survey was assayed by 8 SSR markers and three polymorphic SSR markers viz., RM296, RM585 and OSR30 were selected to evaluate BC1F1 rice lines for salt tolerance. Primer RM585 indicated 16 lines as introgressed and 16 lines as susceptible in comparison with salt tolerant parent FL-378 and salt susceptible parent Binadhan-7. Primer RM296 identified 13 introgressed, and 19 susceptible lines. Nine introgressed and 23 susceptible lines were identified when BC1F1 lines were evaluated with marker OSR30. Line BC1F1-30, BC1F1-40, BC1F1-2, BC1F1-16 and BC1F1-34 were identified as introgressed in comparison with parent FL-378 (salt tolerant) when RM585 and RM296 markers were used but line BC1F1-1 and BC1F1-3 were identified as introgressed incase of 3 primers (RM585, RM296 and OSR30). Again the lines BC1F1-5, BC1F1-24, BC1F1-26, BC1F1-27, BC1F1-29, BC1F11F1-37 and BC1F1(PIC) values ranged from 0.3290 to 0.3671 with an average of 0.3544. The overall gene diversity of SSR loci for the 32 BC1F1 rice lines along with two parents was 0.4614, ranging from 0.4152 to 0.4844. Positive correlations were found between the genetic diversity and the maximum number of repeats. Among the lines, all the loci were polymorphic and clearly distinct and the cluster analysis (NJ tree and UPGMA) showed nearly similar pattern of variation which could be used for improvement of salt tolerant rice lines of rice through SSR markers.DOI: http://dx.doi.org/10.3329/jbau.v10i1.12039J. Bangladesh Agril. Univ. 10(1): 49–54, 2012

scholarly journals Hybrid identification for Glycine max and Glycine soja with SSR markers and analysis of salt tolerance

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6483 ◽  
Author(s):  
Fayuan Li ◽  
Xun Liu ◽  
Shengyan Wu ◽  
Qingyun Luo ◽  
Bingjun Yu
Keyword(s):  
Glycine Max ◽  
Salt Tolerance ◽  
Ssr Markers ◽  
Glycine Soja ◽  
Female Parent ◽  
Tolerance Test ◽  
Dry Matter Accumulation ◽  
Salt Tolerant ◽  
Cross Combination ◽  
Hybrid Identification

Glycine max cultivars Lee68, Nannong 1138-2, and Nannong 8831 were used as the female parents, and hybrid lines (F5) 4,111, 4,076 (N23674 × BB52), 3,060 (Lee68 × N23227), and 185 (Jackson × BB52) that selected for salt tolerance generation by generation from the cross combination of G. max and G. soja were used as the male parents, 11 (A–K) backcrosses or three-way crosses were designed and 213 single hybrids were harvested. The optimized soybean simple sequence repeat (SSR)–polymerase chain reaction (PCR) system was used to analyze the SSR polymorphism of above parental lines and get the parental co-dominant SSR markers for hybrid identification, and in which 30 true hybrids were gained. The true hybrids (G1, G3, G9, G12, G13, G16) of G cross combination were chosen as the representative for the salt tolerance test, and the results showed that, as exposed to salt stress, the seedlings of G9 line displayed higher salt tolerant coefficient, relative growth rate, and dry matter accumulation, when compared with their female parent Nannong 1138-2, and even performed equally strong salt tolerance as the male parent 3,060. It provides a feasible method of the combination of molecular SSR markers and simple physiological parameters to identify the true hybrids of G. max and G. soja, and to innovate the salt-tolerant soybean germplasms.

scholarly journals Antioxidant-enzymatic system of two sorghum genotypes differing in salt tolerance

2005 ◽  
Vol 17 (4) ◽  
pp. 353-362 ◽  
Author(s):  
Paulo Henrique Alves da Costa ◽  
André Dias de Azevedo Neto ◽  
Marlos Alves Bezerra ◽  
José Tarquinio Prisco ◽  
Enéas Gomes-Filho
Keyword(s):  
Superoxide Dismutase ◽  
Salt Stress ◽  
Salt Tolerance ◽  
Enzymatic System ◽  
Salt Tolerant ◽  
Growth Reduction ◽  
Sorghum Genotypes ◽  
Scavenging Enzymes ◽  
Sensitive Genotype ◽  
Tolerant Genotype

Two forage sorghum genotypes were studied: CSF18 (salt-sensitive) and CSF20 (salt-tolerant). Shoot growth reduction as a result of salt stress was stronger in the salt sensitive genotype compared to the salt tolerant one. When the two genotypes were subjected to salt stress (75 mM NaCl) no significant change in lipid peroxidation was observed. However, salt stress induced increases in superoxide dismutase and catalase activities in both genotypes. These salt-induced increases were higher in the salt-tolerant genotype. Peroxidase activity was differentially affected by salt stress in the two genotypes. The activities of these peroxidases were decreased by salt stress in the salt-sensitive genotype and increased in the salt-tolerant genotype. In addition, the activity ratio between the superoxide dismutase and the H2O2-scavenging enzymes was higher in the salt-sensitive genotype. The results obtained support the hypothesis that the higher efficiency of the antioxidant-enzymatic system of the CSF20 genotype could be considered as one of the factors responsible for its tolerance to salt stress. Therefore, it is suggested that the ratio between superoxide dismutase and H2O2-scavenging enzyme activities could be used as a working hypothesis for a biochemical marker for salt tolerance in sorghum.

Molecular Mechanism of Salt Tolerance for Genetic Diversity Analysed in Association with Na+/K+ Ratio through SSR Markers in Rice (Oryza sativa L.)

2010 ◽  
Vol 5 (9) ◽  
pp. 708-719 ◽  
Author(s):  
P. Senguttuve ◽  
M. Raveendran ◽  
C. Vijayalaks ◽  
K. Thiyagaraj ◽  
J.R. Kannan Bapu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Salt Tolerance ◽  
Molecular Mechanism ◽  
Ssr Markers ◽  
Oryza Sativa L

scholarly journals Identification of introgressed rice lines of Binadhan-7×FL-378 under salt stress through SSR markers

2013 ◽  
Vol 10 (2) ◽  
pp. 185-190
Author(s):  
MA Haque ◽  
MA Mahmud ◽  
MM Islam ◽  
SN Begum
Keyword(s):  
Salt Tolerance ◽  
Ssr Markers ◽  
Gene Diversity ◽  
Salt Tolerant ◽  
Bc1f1 Population ◽  
Backcross Population ◽  
Ssr Loci ◽  
Salt Susceptible ◽  
Pattern Of Variation ◽  
Positive Correlations

Thirty two rice lines of BC1F1 population (Binadhan-7/FL 378) were used to identify introgressed rice lines for salt tolerance using SSR markers at the Plant Breeding and Biotechnology Divisions of Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. Seeds of BC1F1 population of Binadhan-7/FL-378 were collected from backcross population of F1 (Binadhan-7/FL 378). Salt tolerant genotype, FL-378 was crossed with high yielding variety, Binadhan-7. Randomly selected 32 BC1F1 progenies along with their two parents (Binadhan-7, FL-378) were genotyped with microsatellite or SSR markers for identification of introgressed rice lines. Parental polymorphism survey was assayed by 8 SSR markers and three polymorphic SSR markers viz., RM296, RM585 and OSR30 were selected to evaluate BC1F1 rice lines for salt tolerance. Primer RM585 indicated 16 lines as introgressed and 16 lines as susceptible in comparison with salt tolerant parent FL-378 and salt susceptible parent Binadhan-7. Primer RM296 identified 13 introgressed, and 19 susceptible lines. Nine introgressed and 23 susceptible lines were identified when BC1F1 lines were evaluated with marker OSR30. Line BC1F1-30, BC1F1-40, BC1F1-2, BC1F1-16 and BC1F1-34 were identified as introgressed in comparison with parent FL-378 (salt tolerant) when RM585 and RM296 markers were used but line BC1F1-1 and BC1F1-3 were identified as introgressed incase of 3 primers (RM585, RM296 and OSR30). Again the lines BC1F1-5, BC1F1-24, BC1F1-26, BC1F1-27, BC1F1-29, BC1F1-31, BC1F1-37 and BC1F1-38 were found susceptible as compared with parent Binadhan-7 when marker RM585, RM296 and OSR30 were used. The polymorphism information content (PIC) values ranged from 0.3290 to 0.3671 with an average of 0.3544. The overall gene diversity of SSR loci for the 32 BC1F1 rice lines along with two parents was 0.4614, ranging from 0.4152 to 0.4844. Positive correlations were found between the genetic diversity and the maximum number of repeats. Among the lines, all the loci were polymorphic and clearly distinct and the cluster analysis (NJ tree and UPGMA) showed nearly similar pattern of variation which could be used for improvement of salt tolerant rice lines of rice through SSR markers. DOI: http://dx.doi.org/10.3329/jbau.v10i2.14680 J. Bangladesh Agril. Univ. 10(2): 185–190, 2012

Sign in / Sign up

Export Citation Format

Share Document