scholarly journals Prevalence and abundance of insect pests in stored pulses collected from two local markets of Dhaka city

2019 ◽  
Vol 8 (1) ◽  
pp. 75-82
Author(s):  
Tangin Akter ◽  
Shanjida Sultana ◽  
Mehjabin Rahman ◽  
Md Reaz Mahmud ◽  
Shefali Begum
Keyword(s):  
Lens Culinaris ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Green Gram ◽  
Callosobruchus Chinensis ◽  
Sitotroga Cerealella ◽  
Dhaka City ◽  
Stored Grain ◽  
Rhizopertha Dominica ◽  
Liposcelis Entomophila

Prevalence and abundance of stored grain insect pests in four varieties of pulses i.e. Lens culinaris, Pisum staivum, Cicer arietinum collected from two markets of Dhaka city were recorded. Nine species of insect pests, viz. Sitophilus oryzae, Tribolium castaneum, Callosobruchus chinensis, C. maculatus, Rhizopertha dominica, Oryzaephilus surinamensis, Liposcelis entomophila, Sitotroga cerealella, and Formica sp., were recorded throughout the study. The percentage of weight-loss was higher (7.54) in those pulses which were highly infested by pests. Highest pest population was observed during May to June (753). However, Sitotroga cerealella were found only in November to December of the study period. The maximum species richness (7) was observed in Green gram and Chickpeas samples, but the minimum species evenness was observed in Green gram (0.6969) and maximum in Red lentil (0.9886). Jahangirnagar University J. Biol. Sci. 8(1): 75-82, 2019 (June)

scholarly journals Molecular characterization and identification of three stored grain pests based on mitochondrial cytochrome C oxidase subunit I (COI) gene sequences

2019 ◽  
Vol 47 (1) ◽  
pp. 1-11
Author(s):  
Abu Faiz Md Aslam ◽  
Sharmin Sultana ◽  
Faria Farhana Rain ◽  
Sumita Rani Das ◽  
Ayesha Siddika ◽  
...  
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Dna Barcode ◽  
Control Measures ◽  
Coi Gene ◽  
Composition Analysis ◽  
Callosobruchus Chinensis ◽  
Pest Species ◽  
Stored Grain ◽  
Stored Grain Pests

Stored grain pests are discovered in food as immature stages, which further complicates the identification process. A DNA barcode dataset of some important pests that can be used for easy and confirm identification in stages of life is constructed. COI genes of three stored grain insect pests i.e,, Sitophilus oryzae, Callosobruchus chinensis and Oryzaephilus surinamensis were sequenced. The sequenced genes were submitted to NCBI GenBank and obtained accession numbers MG967331.1, MG967332.1, MG967333.1 and MK041216.1. BLAST analysis showed 99 to 100% homology with existing GenBank sequences. The nucleotide composition analysis revealed that the value of A+T (64.8%) is greater than G+C (35.2%). Genetic distance among four sequences of three store pests were ranged from 0.00293-0.32807. Phylogenetic analysis showed that these three species are originated from different clades. Haplotype analysis of mitochondrial COI gene of the stored grain insect pests showed high genetic diversity among them. C. chinensis, O. surinamensis and S. oryzae were separated from their common ancestor by 80, 73 and 64 mutational steps. These information may be helpful for attempting any successful control measures against the pest species. In conclusion, present author established the first DNA barcode dataset of three store grain pests and confirmed its efficiency for identifying these pests. Bangladesh J. Zool. 47(1): 1-11, 2019

scholarly journals Preparation, Characterization and Biological Efficacy of Eucalyptus Oil Nanoemulsion against the Stored Grain Insects

2020 ◽  
pp. 41-51
Author(s):  
Tahany G. M. Mohammed ◽  
M. E. H. Nasr
Keyword(s):  
Control Strategies ◽  
Physical Stability ◽  
Sitophilus Oryzae ◽  
Contact Toxicity ◽  
Fumigant Toxicity ◽  
Stored Grain ◽  
Spontaneous Emulsification ◽  
Eucalyptus Oil ◽  
Rhizopertha Dominica ◽  
Stored Grain Insects

Nanotechnology has enormous potential for developing alternative pest control strategies and reducing the risk of insecticide molecules. The present study aimed to develop a stable nanoemulsion (NE) of eucalyptus oil (EO) by the spontaneous emulsification method and evaluate its insecticidal and repellent effect against Sitophilus oryzae (L.), Rhizopertha dominica (F.) and Tribolium-castaneum (Herbst.). The prepared nanoemulsion formulation having a small particle size 8.57 nm with polydispersity index (PDI) 0.28. The study of the stability and physicochemical properties showed that the prepared formulation had good physical stability without any change in the macroscopic parameters. In addition, results showed that theinsecticidal activity of the prepared NE was higher than the original EO against the three tested insect speciesand the mortality increased with increasing concentrations and extending of exposure time. The contact toxicity of NE film revealed that, adults of R. dominica was more susceptible to all treatments followed by S. oryzae adults, while adults of T. castaneum was the least susceptible one, however, adults of S. oryzae was more susceptible followed by T. castaneum adults while, adults of                    R. dominica was the least susceptible one according to the fumigant toxicity and repellency. Our results suggested that the prepared formulation may be used in an integrated pest managementprogramfor controlling stored grain insects.

scholarly journals Phytochemical Action of White Mulberry Extracts against Three Important Stored Grain Pests

2017 ◽  
Vol 9 (4) ◽  
pp. 359-366
Author(s):  
Z. R. Sabuj ◽  
A. Khatun ◽  
M. Abdullah ◽  
D. M. Badruddoza ◽  
H. Ali ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Sitophilus Oryzae ◽  
Stem Bark ◽  
Callosobruchus Chinensis ◽  
Ether Extract ◽  
Stored Grain ◽  
Chcl3 Extract ◽  
Stored Grain Pests ◽  
White Mulberry

Petroleum ether (Pet. ether), CHCl3 and CH3OH extracts of white mulberry were subjected to dose-mortality tests against Callosobruchus chinensis (L.), Sitophilus oryzae (L.) and Tribolium castaneum (Hbst.). Pet. ether extract of  leaves against C. chinensis, S. oryzae and T. castaneum provided LD50 9.16, 5.06, 3.79, 2.81, 2.06, 1.55, 1.32, 1.38 mg/g; 17.6, 12.9, 11.3, 82.4, 83.5, 9.47, 8.73, 8.61 mg/g and 0.93, 0.826, 0.691, 0.623, 0.567, 0.532, 0.525 mg/cm2 after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against C. chinensis Pet. ether extract of root and stem bark gave LD50 28.3, 20.7, 18.4, 16.9, 14.4, 13.6, 13.3, 13.3 mg/g and 86.3, 228.9, 177, 109, 61.0, 21.8, 0.999, 15 mg/g after 0.5, 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. CH3OH extracts of leaves offered LD50 21.5, 20.4, 17, 16.2, 15.6, 14.7 mg/g against C. chinensis; 5.4, 14.7, 13.7, 11, 10.9, 9.46, 9.10 mg/g against S. oryzae; and 3.804, 3.429, 3.284, 2.736, 2.441, 2.311, 2.264 mg/cm2 against T. castaneum after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. Against S. oryzae CH3OH extract of root and CHCl3 of stem bark gave LD50 19.5, 19.1, 17.5, 16.7, 16.2, 15.8, 15.9 mg/g and 9.77, 9.44, 7.71, 6.56, 5.94, 5.39, 5.4 mg/g after 6, 12, 24, 36, 48, 60, 72 h of exposures respectively. However, CHCl3 extract of leaves and root didn’t show mortality.

scholarly journals Composition of the Essential Oil and Insecticidal Activity of Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey Growing in Nigeria

Foods ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 914
Author(s):  
Moses S. Owolabi ◽  
Akintayo L. Ogundajo ◽  
Azeezat O. Alafia ◽  
Kafayat O. Ajelara ◽  
William N. Setzer
Keyword(s):  
Essential Oil ◽  
Insecticidal Activity ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Gas Chromatography Mass Spectrometry ◽  
Oil Composition ◽  
Stored Grain ◽  
Monoterpene Hydrocarbons ◽  
Sesquiterpene Hydrocarbons ◽  
Leaf Essential Oil

The rice weevil (Sitophilus oryzae) is a pest of stored grain products such as rice, wheat, and corn. Essential oils represent a green environmentally-friendly alternative to synthetic pesticides for controlling stored-product insect pests. Launaea taraxacifolia is a leafy vegetable plant found in several parts of Nigeria. The leaves are eaten either fresh as a salad or cooked as a sauce. The essential oil obtained from fresh leaves of L. taraxacifolia was obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC-MS). Twenty-nine compounds were identified, accounting for 100% of the oil composition. The major component classes were monoterpene hydrocarbons (78.1%), followed by oxygenated monoterpenoids (16.2%), sesquiterpene hydrocarbons (2.1%), oxygenated sesquiterpenoids (0.3%), and non-terpenoid derivatives (3.3%). The leaf essential oil was dominated by monoterpene hydrocarbons including limonene (48.8%), sabinene (18.8%), and (E)-β-ocimene (4.6%), along with the monoterpenoid aldehyde citronellal (11.0%). The contact insecticidal activity of L. taraxacifolia essential oil against Sitophilus oryzae was carried out; median lethal concentration (LC50) values of topical exposure of L. taraxacifolia essential oil were assessed over a 120-h period. The LC50 values ranged from 54.38 μL/mL (24 h) to 10.10 µL/mL (120 h). The insecticidal activity of the L. taraxacifolia essential oil can be attributed to major components limonene (48.8%), sabinene (18.8%), and citronellal (11.0%), as well as potential synergistic action of the essential oil components. This result showed L. taraxacifolia essential oil may be considered as a useful alternative to synthetic insecticides.

Toxic and repellent action of sugarcane bagasse-based lignin against some stored grain insect pests

1970 ◽  
Vol 25 ◽  
pp. 27-30 ◽  
Author(s):  
LA Muslima Khanam ◽  
D Talukder ◽  
MA Hye
Keyword(s):  
Tribolium Castaneum ◽  
Sugarcane Bagasse ◽  
Laboratory Experiments ◽  
Callosobruchus Maculatus ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Repellent Activity ◽  
Stored Grain ◽  
Time Intervals ◽  
Class V

Laboratory experiments were carried out to investigate the efficacy of sugarcane bagasse-based lignin against four stored grain insect pests viz., Tribolium castaneum, Tribolium confusum, Sitophilus oryzae and Callosobruchus maculatus. Mortality was assessed after 24, 48 and 72 h of treatment and LD50 values were calculated at the respective time intervals. It was observed that T. castaneum is more susceptible to the lignin than the other test insects. Repellent activity of the product was conducted for T. castaneum and T. confusum. All the concentrations of the product were found to be repellent to both the insect species. Strong repellent activity was observed at concentrations of 471.57 and 628.76 µg.cm-2 to T. confusum showing the repellency of class V activity. Key words: Bagasse-based lignin, toxicity, repellency, Tribolium castaneum, T. confusum, Sitophilus oryzae, Callosobruchus maculatus Univ. j. zool. Rajshahi Univ. Vol.25 2006 p.27-30

Mercury as a Control for Stored Grain Pests

1944 ◽  
Vol 35 (2) ◽  
pp. 143-160 ◽  
Author(s):  
D. W. Wright
Keyword(s):  
Total Surface Area ◽  
Scientific Basis ◽  
Sitotroga Cerealella ◽  
Oryzaephilus Surinamensis ◽  
Stored Grain ◽  
Metallic Mercury ◽  
Storage Container ◽  
Angoumois Grain Moth ◽  
Rhizopertha Dominica ◽  
Stored Grain Pests

The Indian custom of placing metallic mercury with the grain in the storage container to prevent infestation by insects has been investigated. It is found to have a sound scientific basis as the presence of mercury prevents the reproduction of certain of these pests.Experiments showed that the vapour of mercury was fully effective in preventing reproduction of the grain weevil (Calandra granaria), the saw-toothed grain beetle (Oryzaephilus surinamensis), the lesser grain borer (Rhizopertha dominica), and the Angoumois grain moth (Sitotroga cerealella).Zinc and tin amalgams and calomel were less effective than metallic mercury.The efficiency of a given weight of mercury is increased by subdivision, a process which increases its total surface area.

Migration of stored-grain insect pests from a small wheat bulk

1984 ◽  
Vol 24 (125) ◽  
pp. 260 ◽  
Author(s):  
ER Sinclair ◽  
J Alder
Keyword(s):  
Food Source ◽  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Red Flour Beetle ◽  
Rhyzopertha Dominica ◽  
Stored Grain ◽  
Grain Beetles ◽  
Grain Bulks ◽  
Source Populations ◽  
Cryptolestes Pusillus

One hundred unsexed adults of each of the following coleopterous species were added to 27 .3 kg of clean wheat: Sitophilus oryzae (L.) (rice weevil), Rhyzopertha dominica (F.) (lesser grain borer), Tribolium castaneum (Herbst) (rust-red flour beetle), and the flat grain beetles Cryptolestes pusillus (Schonherr), C. ferrugineus (Stephens), and C. pusilloides (Steele and Howe). For 15 months, measurements were made of population size and emigration from this wheat bulk. The highest populations recorded for each species were: 138 850, 65 680, 9 060, 47 000, 15 530, 11 940, respectively, and total emigrant numbers during the period were: 774 682, 278 094, 123 784, 335 588, 39 070, 9 352, respectively. Such high numbers of emigrants in relation to relatively low source populations emphasize the significance of small amounts of infested grain as sources of infestation of large grain bulks, and hence the importance of grain hygiene. The proportion of females in the populations of four species changed significantly from 0.50 as populations developed. Emigration behaviour was influenced most commonly by temperature, insect numbers, and wheat age, but these factors did not act uniformly on all species. Negligible numbers of emigrants returned to the food source. Low numbers caught in food traps away from the source were consistent with these not being attractive but traps near the shed walls caught greater numbers than expected.

scholarly journals Insecticidal effect of from three Hypericum species extracts against Rhyzopertha dominica, Sitophilus oryzae and Tribolium confusum

2021 ◽  
Vol 45 ◽  
Author(s):  
Cennet Yaman ◽  
Şeyda Şimşek
Keyword(s):  
Insect Pests ◽  
Sitophilus Oryzae ◽  
Tribolium Confusum ◽  
Rhyzopertha Dominica ◽  
Botanical Insecticides ◽  
Stored Grain ◽  
Leaf Extracts ◽  
Insecticidal Effect ◽  
Hypericum Species ◽  
Control Insect

ABSTRACT The search for new plant natural products with insecticidal properties to control insect pests in agriculture has gained relevance in the past decades. The aim of the study was to investigate the insecticidal activity of extracts derived from flower, leaf, and stem of three Hypericum species (Hypericum heterophyllum, Hypericum perforatum, Hypericum scabrum) against the adults of three important stored grain insect pests namely; Sitophilus oryzae (Curculionidae), Rhyzopertha dominica (Bostrichidae) and Tribolium confusum (Tenebrionidae). The insects were incubated with the food under 10% concentration of Hypericum extracts and the mortality was recorded after 24, 48 and 72 h of exposure. The extracts of the Hypericum species and exposure time were found to have statistically significant effective against the three insect pests. After 72 h exposure, the mortality ranged from 4.3 to 94.1 % for all insects. Among tested insects, R. dominica was more susceptible than T. confusum and S. oryzae. Although desirable insecticidal effect against the insects were recorded from all the three Hypericum species, the leaf extract of H. perforatum was more effective on R. dominica, while the flower and stem of H. scabrum displayed high toxic effect on T. confusum and S. oryzae, respectively. The leaf extracts, of H. perforatum, in particular, may be used as source of new potential botanical insecticides against R. dominica in stored grains.

scholarly journals EFFICIENCY OF ORGANIC AND INORGANIC PESTICIDES AGAINST STORED GRAIN INSECT PESTS

2021 ◽  
Vol 37 (2) ◽  
pp. 110-117
Author(s):  
J. Zaib ◽  
S. A. Memon ◽  
A. Ali ◽  
G. A. Bugti ◽  
G. Khaliq
Keyword(s):  
Insect Pests ◽  
Red Flour Beetle ◽  
Control Group ◽  
Callosobruchus Chinensis ◽  
Neem Seed ◽  
Stored Grain ◽  
Flour Beetle ◽  
Powder Concentration ◽  
Neem Leaves ◽  
Pulse Beetle

The insect pests cause substantial loss of grains in the field and during storage of food grains that worth millions of rupees. Hence, in order to manage stored grain insect pests a combination of organic and inorganic pesticide control materials was selected to check the efficiency against red flour beetle (Tribolium castaneum) and pulse beetle (Callosobruchus chinensis). Total 10 treatments were used, eight different organic bio-pesticides (neem seed, neem leaves, naswar, tobacco, eucalyptus, citrus, dhatura and mint) and one inorganic (naphthalene balls) and control used at 32 ± 2°C and 70 ± 5% RH. The results indicated that three doses of all treatments were used as 5, 10 and 20gm in 50gm grains. Overall, maximum (4.33 ± 1.00%) mortality of red flour beetle was observed using neem seed powder and no (0.0 0 ± 00%) mortality was observed in minte leaves powder. In case of pulse beetle, the maximum (5.07 ± 0.87%) mortality was observed using neem seed powder and minimum (0.66±0.22%) using citrus leaves powder. Maximum (16.26 ± 0.03) antifeedant efficiency of red flour beetle was observed in control group and minimum (6.46±0.65) antifeedant efficiency of red flour beetle was found in dhatura leaves powder at all tested concentrations. Maximum (14.23 ± 0.13) antifeedant efficiency of pulse beetle was determined in control group and and minimum (1.00 ± 0.04) was observed for naphthalene balls at all tested concentrations. Maximum (94.67%) repellency against red flour beetle was observed using neem seed powder and minimum (62.00%) was found in naphathalene balls as compared with their controls. Maximum (81.32%) repellency against pulse beetle was observed using naswar powder and minimum (63.34%) repellency was determined using neem leaves powder as compared with their control group. We concluded that 20% of neem seed powder concentration proved effective to suppress the population of both red flour beetle and pulse beetle.

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