scholarly journals Relevance of mast cell infiltration in well-differentiated oral squamous cell carcinoma in biopsy specimen

Mediscope ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 67-74
Author(s):  
Syeda Noorjahan Karim ◽  
Prasun Biswas ◽  
ABM Iftekhar Hossain ◽  
AFM Saleh ◽  
Md Noor Nabi Sayem
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cross Sectional Study ◽  
P Value ◽  
Cross Sectional ◽  
Oral Cancers ◽  
Well Differentiated

Oral cancer is very common worldwide. More than 90% of all oral cancers are squamous cell carcinoma (SCC). The molecular biological markers of oral SCC have been extensively studied to aid in prevention and prognosis. However, no marker has been universally accepted so far. Mast cells are important component of cancer stromal interaction. Their early recruitment in tumor microenvironment and multifarious function make them a burning topic of interest in the field of research. So mast cell may act as a new target for the adjuvant treatment of oral SCC. Therefore, the aim of the study was to assess the number of mast cell infiltrations in well differentiated oral SCC. In this cross-sectional study the sample size was 66. After routine tissue processing and staining with Hematoxylin & Eosin (H/E) stains slides of all cases were grouped as well differentiated oral SCC. Identification of mast cell was done by Toluidine blue stain. Distribution of mast cells was observed and number of mast cells was counted. The data was tabulated and statistical analysis was performed. The number of mast cell was highly increased in all cases of oral SCC than normal. The mean ± SD number of mast cells was 3.28 ± 1.21 in oral SCC, whereas in control oral tissue it was 0.44 ± 0.19. The p-value was found to be highly significant (p<0.001). So, in our observation mast cell is a good cellular indicator of tumorigenesis. Mediscope 2021;8(2): 67-74

scholarly journals IMMUNOEXPRESSION OF CD-10 IN BASAL CELL CARCINOMA AND SQUAMOUS CELL CARCINOMA OF HEAD AND NECK

2021 ◽  
Vol 71 (6) ◽  
pp. 2175-79
Author(s):  
Maria Ilyas ◽  
Muhammad Yasir Rafiq ◽  
Farha Farhan ◽  
Misbah Ali ◽  
Saeed Afzal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Basal Cell ◽  
Armed Forces ◽  
Cross Sectional Study ◽  
Ethical Review ◽  
P Value ◽  
Cross Sectional

Objective: To compare the expression of CD-10 in basal cell carcinoma (BCC) and squamous cell carcinoma (SCC). Study Design: Comparative cross-sectional study. Place and Duration of Study: Armed Forces Institute of Pathology, Rawalpindi, from Jun 2016 to Jun 2017. Methodology: After taking ethics approval from the ethical review committee AFIP, previously diagnosed, thirty-eight cases of each basal cell carcinoma and squamous cell carcinoma were taken from the histopathology department of Armed Forces Institute of Pathology, Rawalpindi. CD-10 was applied according to standard protocol. The intensity of the CD-10 expression is assessed and categorized into three groups. CD-10 expression less than 10% in the tumour cell was taken as negative (-), 10 to 50% staining was taken as positive (+) and greater than 50% staining in tumour cells was taken as strong positive (++). Results: Out of the total 76 cases included in the study, 9 (24%) were strongly positive (++) whereas 20 (52.6%) were positive (+) and 9 (23.7%) were negative (-) for CD-10 expression. Out of the total 38 cases of SCC 4 (11%) cases showed positive CD-10 expression and 34 (89%) showed negative (-) results. A significant association (p-value <0.001) was seen when CD-10 expression in BCC was compared with SCC. Conclusion: CD-10 expression was higher in basal cell carcinoma when compared to squamous cell carcinoma in which CD-10 expression was very low/absent, hence CD-10 can serve as a useful adjunct marker in differentiating between basal cell carcinoma and squamous cell carcinoma in problematic cases.

scholarly journals Iron deficiency and risk of oral squamous cell carcinoma

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Imrana Tanvir ◽  
Rizwan Ullah Khan ◽  
Amber Hassan ◽  
Sami Ullah Mumtaz
Keyword(s):  
Squamous Cell Carcinoma ◽  
Iron Deficiency ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Young Patients ◽  
Cross Sectional Study ◽  
Cross Sectional ◽  
Well Differentiated ◽  
Age Range

Oral squamous cell carcinoma (OSCC) is a subset of head and neck squamous cell carcinoma caused by excessive substance abuse like alcohol, tobacco etc.  Objectives: Aim of the present study was to evaluate the iron expression in different grades of OSCC and potential of iron staining as a prognostic marker and its importance as an essential nutrient in diet. Methods and Patients: It was a cross sectional study. A total of 40 oral biopsies were evaluated and the mean age of patients was 53.5 years with age range 23-80 years. 14 cases were well differentiated, 14 moderately differentiated and there were 12 poorly differentiated case. Iron staining was performed for all these cases. Results: Iron positivity was observed in 11 (27.5%) of OSCC patients with maximum iron positivity in well differentiated group (64.28%) of age range 20-40 years (70%). More patients had tongue as site of cancer. There was no genderwise difference in iron expression. Iron deficiency was associated with poor prognosis indicating iron as an important nutrient which can prevent OSCC if adequately present in our routine diet. Conclusions: Prevalence of iron positivity in young patients and initial stage of OSCC, indicates the protective effect of iron against oral squamous cell carcinoma

scholarly journals Immunohistochemical Expression of EGFR, NF-kB and Cyclin D1 in Sinonasal Inverted Papilloma and Squamous Cell Carcinoma

2020 ◽  
Vol 12 (3) ◽  
pp. 239-44
Author(s):  
Pudji Rahaju ◽  
Rio Auricknaga Kintono ◽  
Ahmad Dian Wahyudiono ◽  
Arif Satria ◽  
Ferry Sandra
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cyclin D1 ◽  
Squamous Cell ◽  
Growth Factor Receptor ◽  
Neck Surgery ◽  
Cross Sectional Study ◽  
Inverted Papilloma ◽  
Cross Sectional ◽  
Sinonasal Inverted Papilloma

BACKGROUND: Sinonasal inverted papilloma (SIP), a benign epithelial growth in the sinonasal region with epidermoid epithelial transformation, has been known for its invasiveness, recurrency, and its link with malignancy. Meanwhile sinonasal squamous cell carcinoma (SSCC) is an epithelial malignancy on squamous cells from the sinonasal region. Epidermal growth factor receptor (EGFR), Nuclear Factor kB (NF-kB), and Cyclin D1 are factors those might play important role in proliferation of SIP and SSCC. This research was conducted to investigate the expressions of EGFR, NF-kB and Cyclin D1 in SIP and SSCC.METHODS: A cross-sectional study by examining the EGFR, NF-kB, and Cyclin D1 immunohistochemical expressions of SIP and SSCC was conducted. Subjects whose blocks were used in this research, were diagnosed as SIP and SSCC at the Otorhinolaryngology-Head and Neck Surgery Clinic, Dr. Saiful Anwar General Hospital. Samples were selected, processed for inmmunohistochemistry, evaluated and statistical analyzed.RESULTS: Twenty-four SIP and 9 SSCC subjects with their paraffin blocks were selected. Clear immunohistochemical expressions of EGFR, NF-kB, and Cyclin D1 were observed for both SIP and SSCC. Significantly higher immunostaining levels of EGFR (45.6%, p=0.001) and NF-kB (42.2%, p=0.013) were observed in SSCC. Immunostaining levels of EGFR vs. NF-kB were moderately correlated (p=0.03, r=0.437), while the immunostaining levels of NF-kB vs. Cyclin D1 were strongly correlated (p=0.002, r=0.602).CONCLUSION: Expression of EGFR and NF-kB in SSCC were higher than the EGFR and NF-kB expression in SIP, suggesting that EGFR and NF-kB play important role in sinonasal malignancy.KEYWORDS: sinonasal, inverted papilloma, SCC, EGFR, NF-kB, Cyclin D1

scholarly journals Altered Coagulation Pattern in Different Histological Grades of Squamous Cell Carcinoma

2020 ◽  
Vol 9 (2) ◽  
pp. 123-128
Author(s):  
Kiran Shaikh ◽  
Ghulam Shah Nizamani ◽  
Yar Muhammad Nizamani ◽  
Naila Nizamani ◽  
Amin Fahim ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Age Groups ◽  
University Hospital ◽  
High Rate ◽  
Cross Sectional ◽  
Coagulation Profile ◽  
Poorly Differentiated ◽  
Well Differentiated

Background: The incidence of squamous cell carcinoma has doubled in the last three decades associated with a high rate of morbidity and mortality. The incidence of bleeding and/or thrombosis in advanced stage cancer necessitates a need for research in blood coagulation abnormalities in malignancy. The objective of the present study was to determine the alteration in coagulation pattern among patients diagnosed with squamous cell carcinoma (SCC). Material and Methods: This cross-sectional study was conducted in Pathology department of Isra University Hospital, Hyderabad over a period of 06 months. A total of 126 samples were selected through non-probability convenient sampling. Both male and female patients of all age groups having SCC of skin, gastrointestinal and genital tract were included. The coagulation profile was analyzed by Sysmax CA 50 and Nycocard reader II. Data was entered into SPSS version 22.0 and results were analyzed. Results: The mean age of the patients with SCC in the present study was 55.5 ± 12 years with more male patients (n=81; 64.3%) as compared to females (n=45; 35.7%). Oral SCC was found to be the most common site of squamous cell carcinoma (43.7%). Majority of the patients had well differentiated SCC (42.1%) followed by moderately differentiated (40.5%) and poorly differentiated SCC (17.4%). The comparison of severity of SCC with coagulation profile revealed that PT (P=0.01), APTT (P=0.001), D-dimers (P=0.01 and TT (P=0.01) were significantly increased, whereas fibrinogen was significantly decreased (P=0.001). Conclusions: The histological differentiation of SCC (from well differentiated to poorly differentiated tumors) showed a highly significant association with different coagulation profile parameters like PT, APTT, BT, CT, D-dimer, TT, and fibrinogen levels.

scholarly journals THE INSIDENCE OF BLADDER SQUAMOUS CELL CARCINOMA IN LARGE BLADDER STONE CASES

2015 ◽  
Vol 22 (1) ◽  
Author(s):  
Joko Pitoyo ◽  
Ferry Safriadi
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cross Sectional Study ◽  
Study Data ◽  
Bladder Stone ◽  
Surgical Removal ◽  
Stone Size ◽  
Cross Sectional ◽  
Anatomical Pathology

Objective: To determine the incidence of squamous cell carcinoma (SCC) in patients with large bladder stone (> 40 mm). Material & method: This is a descriptive retrospective cross-sectional study, data was collected from medical record on patients with a diagnosis of large bladder stone (> 40 mm) that have been performed surgical removal of stone (vesicolithotomy) and bladder mucosa biopsy in Hasan Sadikin Hospital Bandung during 2006-2010. Furthermore, the data are classified according to pathology. Results: This study found cases of large bladder stone (> 40 mm) of 47 cases during the period 2006-2010, and of these 47 cases, there were 76.7% and 23.3% non-malignancy of a malignancy in 2 cases (4.3%) with anatomical pathology picture of SCC. Based on the age group of patients aged 31-40 years obtained 25.5%, 41-50 years 21.3%, 51-60 years 21.3% and > 60 years 23.4%, whereas for ages below 30 years 8.5%. Based on sex, male 95.7% and 4.3% of women. Based on the size, found a stone the size of 40 mm (48.9%), stone size of 50 mm (10.6%), stone size 60 mm and 70 mm respectively (8.5%), stone size 80 mm (12.8%), stone size > 80 mm (4.3%) and 6.4% with multiple bladder stones. Conclusion: There were only two cases of bladder SCC from 47 cases patients with large bladder stone (> 40 mm) in Hasan Sadikin Hospital Bandung during 2006-2010. Keywords: Bladder Stone, bladder carcinoma, squamous cell carcinoma.

scholarly journals ORAL SQUAMOUS CELL CARCINOMA

2015 ◽  
Vol 22 (01) ◽  
pp. 018-026
Author(s):  
Sofia Ali Syed ◽  
Mulazim Hussain Bukhari ◽  
Riaz Ahmed Warraich ◽  
Fauzia Quadir ◽  
Asifa Iqbal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Maxillofacial Surgery ◽  
Nucleolar Organizer Regions ◽  
Pathology Laboratory ◽  
Cross Sectional ◽  
Poorly Differentiated ◽  
Well Differentiated

Background: The aim of this study was to evaluate clinico-pathologicalparameters and the status of argyrophilic nucleolar organizer regions stain in various histologicalgrades of oral squamous cell carcinoma. Materials and Methods: A cross sectional studywas conducted on fifty cases of oral squamous cell carcinoma. The specimen were collectedfrom the department of Oral & Maxillofacial Surgery and processed for hematoxylin and eosinstain and AgNOR stain Pathology Laboratory, King Edward Medical University Lahore. Results:Bidi smoking is associated with oral squamous cell carcinoma. The AgNOR (mAgNOR andpAgNOR) status was significantly low in well differentiated and moderately differentiatedcompared to poorly differentiated oral squamous cell carcinoma (p =0.001). AgNOR size inpoorly differentiated was significantly higher than the AgNOR size in well differentiated oralsquamous cell carcinoma. Similarly the distribution of AgNOR in moderately and poorlydifferentiated oral squamous cell carcinoma was significantly high. The AgNORs index wassignificantly high in poorly differentiated squamous cell carcinoma as compared to welldifferentiated and moderately differentiated squamous cell carcinoma. Conclusions: The useof AgNORs stain is easy, valid and reliable method to assess the histological grading of oralsquamous cell carcinoma and should be used to predict the prognosis of patients.

Prognostic potential of CK-19 managing disseminated oral squamous cell carcinomas in Pakistan: A descriptive cross-sectional study

2020 ◽  
pp. 1-13
Author(s):  
Waqas Iqbal ◽  
Uzma Tariq ◽  
Arhama Surwaich ◽  
Surwaich Ali channa ◽  
Abdul Majid ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cross Sectional Study ◽  
Squamous Cell Carcinomas ◽  
Sectional Study ◽  
College Hospital ◽  
Cross Sectional ◽  
Oral Squamous Cell Carcinomas

Abstract A cross-sectional study was conducted to assess the adoptability of CK-19 as a routine diagnostic assay and potential prognostic marker following disseminated oral squamous cell carcinoma in Pakistani population. The current descriptive study was conducted at Isra Dental College Hospital, Isra University, Hyderabad, Pakistan. Suspected patients of oral squamous cell carcinomas (OSCC), who visited the Isra Dental College Hospital’s outpatient department from January 2014 up to January 2015 with four year follow up (from January 2015 up to December 2019), were included after ethical approval of the Institutional board. SPSS version 21.0 was used for data analysis. Sixty cases of oral squamous cell carcinoma (OSCC) were selected for CK-19 quantification by using PCR before and after incisional biopsy.    

scholarly journals Mast Cells and Angiogenesis in Oral Malignant and Premalignant Lesions

2008 ◽  
Vol 2 (1) ◽  
pp. 126-132 ◽  
Author(s):  
E.Z Michailidou ◽  
A.K Markopoulos ◽  
D.Z Antoniades
Keyword(s):  
Squamous Cell Carcinoma ◽  
Mast Cell ◽  
Mast Cells ◽  
Cell Carcinoma ◽  
Oral Mucosa ◽  
Squamous Cell ◽  
Premalignant Lesions ◽  
Angiogenic Switch ◽  
Normal Oral Mucosa ◽  
Human Cd34

Mast cell contribution to neoangiogenesis during tumorigenesis in oral squamous cell carcinoma is not determined yet. Objectives: To associate numerical mast cell density (MCD) to numerical microvessel density (MVD) during the progression of oral leukoplakia without dysplasia and leukoplakia with dysplasia to squamous cell carcinoma (OSCC). Materials and methods: MVD was analysed immunohistochemically (mouse monoclonal anti-human CD34) in 49 paraffin-embedded specimens, 35 OSCCs, 9 leukoplakias and 5 normal oral tissues. Toluidine blue counterstaining revealed mast cells. MCD and MVD were assessed at the same optical field. Results: MVD increased between: normal oral mucosa, dysplasia (p=0.004), OSCC (p=0.001), leukoplakia and OSCC (p=0.041). MCD increased between: normal oral mucosa, dysplasia (p=0.003), OSCC (p=0.000), leukoplakia and OSCC (p=0.007). MVD was found to depend on MCD (p=0.000) in a percent 28.3% (power curve fit model). Conclusions: Mast cells are attracted at the lesion site and may turn on an angiogenic switch during tumorigenesis in OSCC.

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