Association between Foxp3 Tumor Infiltrating Lymphocyte Expression and Response After Chemoradiation in Nasopharyngeal Carcinoma

BACKGROUND: Nasopharyngeal carcinoma (NPC) is a carcinoma originating from the surface epithelium of the nasopharynx with the highest incidence in China and South East Asia. Currently, many researchers are developing tumor microenvironment which can be assessed by tumor-infiltrating lymphochyte, and its association with treatment response in several tumors, including NPC. Foxp3, known as a regulatory T cell (Treg) marker, plays a role in the immunoregulatory environment of tumor cells and can be used as a prognostic factor. The relationship between Foxp3 expression and treatment response is considered as one of the factors affecting the prognosis of NPC. AIM: This study aims to determine the relationship between Foxp3 expression and treatment response in NPC. MATERIALS AND METHODS: A cross-sectional study was done to analyze the association between Foxp3 and treatment response in NPC. This study included 60 samples who were diagnosed with non-keratinizing NPC at the Department of Anatomical Pathology, Faculty of Medicine Universitas Indonesia/Cipto Mangunkusumo Hospital from January 2018 until December 2020. Immunohistochemistry was done to evaluate the expression of Foxp3. Foxp3 expression was evaluated in the intratumoral and peritumoral areas. RESULTS: Among 60 patients, the number of males were more than females (66.7%, 33.3%, respectively) with a ratio of 2:1. There was statistically significant difference between intratumoral and total Foxp3 expression and treatment response (p < 0.05, p = 0.001, respectively); however, no significant differences found between peritumoral Foxp3 expression and treatment response (p = 0.114). CONCLUSION: Foxp3 expression had a statistically significant relationship with response therapy after chemoradiation.

Microscopic Profile of Mice Liver Tissue (Mus musculus) Fixed with Neutral Buffered Formalin (NBF 10%) and Helly Solution

The fixation solution that is widely used in anatomical pathology laboratories is NBF 10%, the excess of NBF 10% because the pH is close to neutral, can be stored in large quantities and a long time. Helly's fixation solution is a good fixation solution for the cytoplasm, and only requires 2-3 hours of fixation. Knowing the microscopic picture of the preparation of hepar mencit tissue (Mus musculus) fixated with NBF 10% and Helly solution. This research is an experimental study with a descriptive analysis approach. picture of hepar mencit tissue preparation (Mus musculus) fixation with NBF 10% obtained as much as 100% good preparation. While the fixated with Helly solution obtained as much as 66% good preparation. Conclusion: Microscopic picture of hepar mencit tissue preparation (Mus musculus) fixated with NBF is 10% better than Helly solution.

Histopathological Review and Distribution of Granulomatous Inflammatory Disorders in Makurdi, North Central Nigeria

Granulomatous inflammation is a pattern of chronic inflammation characterized by the presence of granulomas consisting of microscopic aggregates of macrophages transformed into epithelioid cells surrounding by a collar of lymphocytes and occasioned plasma cells. The study aimed to determine the distribution by tissue site, histological patterns of granulomatous inflammatory disorders in Benue State University Teaching Hospital (BSUTH), Makurdi over a 5-year period. This was a 5 year (March 2013 – February 2018) retrospective study of all cases diagnosed as granulomatous inflammatory disorders on Haematoxylin and Eosin-stained sections at the Anatomical pathology Department. Ziehl-Neelsen special stain was also done on all specimens. A total of two hundred and thirty-six (236) cases of granulomatous inflammatory disorders were diagnosed, analysed and categorized based on the tissue site, on Haematoxylin and Eosin-stained sections; Ziehl-Neelsen special stain was done on all cases. The most common sites were cervical lymph nodes, appendix, skin, testicular tissues etc with the Eyelid been the least common site.

Three Years Retrospective Study of Melanocytic Lesion in Tertiary Hospital: Comparing Benign & Malignant Data

Melanocytic lesion is defined as skin symptom due to proliferation of melanocytes. It may be considered benign, commonly diagnosed as melanocytic nevus (MN), or may also be malignant as malignant melanoma (MM). Publication of epidemiologic data about melanocytic lesion in Indonesia is limited. The aim of this study was to evaluate the epidemiologic findings of melanocytic lesion based on histopathology and clinical data. This descriptive study was done by collecting retrospective pigmented lesion from histopathology database at Department of Anatomical Pathology dr. Cipto Mangunkusumo National Hospital from 2014 - 2017. Retrieved data were analyzed descriptively for MN and MM include clinical diagnosis, age, sex, location, size and clinical working diagnosis. There were 121 cases of melanocytic lesions consisting of 87.6% MN. Females were more frequent for benign lesions. MN cases were mostly seen at the age below the fourth decade, while from 12 cases of MM found above fourth decade. MN was located mostly on the head and neck, while MM mostly in the lower extremities and soles. There were 75 lesions size and 4 lesions location were unknown data missing. MM is still considered rare. Completing clinical finding in the histopathology request form by surgeons will aid in defining better characteristic of melanocytic lesion in our population. Keyword: epidemiology, melanocytic lesion, nevus melanocytic, malignant melanoma. Studi Retrospektif Lesi Melanositik dalam Tiga Tahun di RS Tersier: Perbandingan Data Jinak dan Ganas Abstrak Lesi melanositik didefinisikan sebagai lesi kulit akibat proliferasi melanosit. Lesi melanositik dapat dianggap jinak, umumnya didiagnosis sebagai nevus melanositik (NM), atau merupakan ganas yaitu melanoma maligna (MM). Publikasi data epidemiologi tentang lesi melanositik di Indonesia masih terbatas. Tujuan dari penelitian ini adalah untuk melakukan evaluasi gambaran epidemiologis lesi melanositik berdasarkan data histopatologi dan klinis. Penelitian deskriptif ini dilakukan dengan mengumpulkan data retrospektif lesi berpigmen dari basis data histopatologi di Departemen Patologi Anatomi Rumah Sakit Nasional dr. Cipto Mangunkusumo dari tahun 2014 - 2017. Data dianalisis secara deskriptif untuk NM dan MM termasuk diagnosis kerja, usia, jenis kelamin, lokasi, ukuran, dan diagnosis klinis. Terdapat 121 kasus lesi melanositik yang terdiri atas 87,6% NM. Lesi jinak lebih banyak ditemukan pada wanita. Terdapat 75 dan 4 kasus dengan data ukuran lesi dan lokasi tidak tercantum. MM masih dianggap jarang. Kasus NM sebagian besar terlihat pada usia di bawah dekade keempat, sedangkan dari 12 kasus MM di atas dekade keempat. NM sebagian besar terletak di kepala dan leher, sedangkan MM ditemukan di ekstremitas bawah dan telapak kaki. Melengkapi temuan klinis dalam formulir permintaan histopatologi oleh dokter bedah akan membantu dalam menentukan karakteristik lesi melanositik lebih baik pada populasi ini. Kata kunci: epidemiologi, lesi melanositik, nevus melanositik, melanoma maligna

Canadian Association of Neuropathologists Workforce Survey, 2019

To characterize the professional occupation of Canadian neuropathologists and estimate the future employment demands in neuropathologists, all the active members of the Canadian Association of Neuropathologists in Canada (n=53) were surveyed by E-mail, inquiring as to their estimated date of retirement, their current employment and practice profile, and as to any practice trends they had noticed. 49 members replied: all but one practice at medical school centers. 38 practice exclusively in neuropathology and three of these are employed at less than 75% of a full time equivalent. The remaining practices are mixed neuropathology and anatomical pathology, and one practices exclusively ophthalmic pathology. 35% reported significant neuropathology sub specialization (e.g. forensic, pediatric, neuromuscular). 42% reported greater than 10% of time dedicated to research (of these, median 30%) and 35% greater than 10% time spent in teaching, and 9% greater than 10% time in administration. Of the 49 surveyed, as of the spring of 2019, 14%(seven) of the full time neuropathologists can be expected to retire in the next 10 years, and 6% (three) with mixed AP/NP practices.LEARNING OBJECTIVESThis presentation will enable the learner to: 1.Understand the current spectrum of practice of Neuropathologists across Canada2.Describe the patterns of employment and anticipated retirements of Canadian Neuropathologists

GAMBARAN SEL EPITEL PADA LESI PAYUDARA DILABORATORIUM PATOLOGI ANATOMI UPT RSUD DELI SERDANG LUBUK PAKAM

The breast is an organ that grows as a tissue bulge that is first seen at 7 to 8 weeks after conception. Each breast consists of 12 to 20 tubuloalveolar gland lobules. Each lobule consists of cells consisting of cube epithelial cells and myoepithelial cells that surround the lumen. Lesions in the breast can originate from a connective tissue or from epithelial cells that develop continuously. Some types of epithelial cell traces in breast lesions include fibroadenoma, tumor filoides, papillomas, and papillary carcinoma, and breast carcinoma. This study aims to determine the description of epithelial cells in breast lesions in the Anatomical Pathology Laboratory of UPT Deli Serdang Lubuk Pakam Hospital Period 2018 - 2019. The research was conducted descriptively using the Fine Needle Aspiration Biopsy (FNAB) examination method. The study was conducted using secondary obtained from the medical record of the Anatomical Pathology Laboratory of UPT Deli Serdang Lubuk Pakam Hospital about the description of epithelial cells in breast lesions. The results showed that there were 6 epithelial cell features in breast lesions in the Anatomy Pathology Laboratory of UPT Deli Serdang Hospital. The six epithelial cell features include mammary aberans, mammary adenoma fibrio, mammary dysplasia, mammary multiple fibroadenoma, mammary ductal hyperplasia and mammary carcinoma. From a total of 30 study samples obtained 14 epithelial cells in the form of mammary adenoma fibrio (46.67%). Description of epithelial cells in the form of mammary aberans as many as 7 people (23.33%). For depictions of epithelial cells in the form of mammary carcinoma by 6 people (20%). While the epithelial picture in the form of mammary dysplasia, multiple fibroadenoma mammary and mammary ductal hyperplasia each as much as 1 person (3.33%).