scholarly journals Authentication of Silybum marianum Varieties Using RAPD Analysis

2014 ◽  
Vol 24 (1) ◽  
pp. 57-63 ◽  
Author(s):  
Sameh Abou Zid
Keyword(s):  
Medicinal Plant ◽  
Plant Tissue ◽  
Rapd Analysis ◽  
Silybum Marianum ◽  
Simple Method ◽  
Polymorphic Bands ◽  
Important Medicinal Plant ◽  
Rapd Method

Silybum marianum is an important medicinal plant used for hepatic disorders. Two varieties exist for this plant S. marianum var. album (white-flowered) and S. marianum var. purple (purple-flowered). A fast and simple method (RAPD) was developed for authentication of S. marianum varieties (purple- and white- flowered) at a DNA level. The two varieties were distinguished by polymorphic bands generated by four decamer primers, namely, OPP-10, OPG-03, OPG-01, and OPC-17. The developed RAPD method will be useful for practical authentication of S. marianum varieties and their adulterants. Plant Tissue Cult. & Biotech. 24(1): 57-63, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19200

scholarly journals The genetic potential of Taxus sumatrana medicinal plants in Kerinci Regency

2021 ◽  
Vol 914 (1) ◽  
pp. 012076
Author(s):  
D Frianto ◽  
I Prihartini ◽  
E Novriyanti ◽  
A Y P B C Widyatmoko
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plants ◽  
Medicinal Plant ◽  
Rapd Analysis ◽  
Polymorphic Locus ◽  
Dna Amplification ◽  
Shannon Index ◽  
Genetic Potential ◽  
Ctab Method ◽  
Rapd Method

Abstract Taxus -a taxol-producing medicinal plant that mostly found in highland area- is a species in genus Taxus and family Taxaceae. This study was aimed to determine the genetic diversity within and between population of T. sumatrana in Kerinci Regency, i.e. Mount Kerinci and Mount Tujuh, based on altitude. The genetic diversity was analyzed with RAPD analysis. The altitude was categorized as low (<2000 m asl) and high (>2000 m asl). The cambium extraction was carried out based on CTAB method. DNA amplification was conducted in RAPD method on machine of PCR System 9700 Applied Biosystems. Nine RAPD primers were used in this study. The results revealed that the average of polymorphic locus was 53.89%. Genetic diversity within population was fairly high with value of 0.1799 and Shannon index of 0.2746. Among the four populations, the population of High Tujuh showed the highest level of variability (He=0.2044). The Nei genetic distance between populations was ranging from 0.0567 to 0.1302. The potential of High Tujuh population is still large enough so that it can still be explored for genetic conservation and cultivated as a taxol-producing material which is useful for medicine.

scholarly journals The use of RAPD markers for differentiation of grapevine varieties registered in the Czech Republic

2011 ◽  
Vol 31 (No. 3) ◽  
pp. 96-101
Author(s):  
K. Moravcová ◽  
M. Baránek ◽  
M. Pidra
Keyword(s):  
Czech Republic ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Initial Screening ◽  
The Czech Republic ◽  
Polymorphic Bands ◽  
Close Relatives ◽  
Rapd Method

Fifty-one grapevine cultivars registered in the Czech Republic were a subject of RAPD analysis. Nine grapevine varieties which were supposed to be genetically distant, were analysed by means of 120 Operon primers during the initial screening of RAPD method. Sixteen primers producing reproducible and polymorphic bands were then used for amplification of all registered varieties. A dendrogram of genetic relationships and a methodology of identification of each variety were created. The resultant dendrogram mostly corresponds with known information about the origin and genetic relationships of the varieties. It is necessary to use the combination of RAPD markers for identification of each variety. It was possible to identify 26 out of 51 varieties registered in the Czech Republic by RAPD markers without any problems. Nine varieties were identifiable only on the basis of present and absent markers. Some varieties were very close relatives &ndash; they formed remote clusters. It was not possible to distinguish between them by RAPD method. Two varieties were not identifiable by the designed key. &nbsp;

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

scholarly journals QUANTIFICATION OF MAJOR PHYTOCHEMICALS OF SWERTIA CHIRAYITA, A MEDICINAL PLANT FROM NEPAL

1970 ◽  
Vol 17 ◽  
pp. 59-68 ◽  
Author(s):  
Susanna Phoboo ◽  
Marcia Da Silva Pinto ◽  
Prasanta C. Bhowmik ◽  
Pramod Kumar Jha ◽  
Kalidas Shetty
Keyword(s):  
Medicinal Plant ◽  
High Performance ◽  
Cultivated Plants ◽  
Swertia Chirayita ◽  
Plant Parts ◽  
Central Nepal ◽  
Significant Difference ◽  
Cultivated Plant ◽  
Array Detection ◽  
Important Medicinal Plant

Swertia chirayita is an important medicinal plant from Nepal with anti-diabetic, anti-pyretic, anti-malarial and anti-inflammatory potential and used in therapeutic herbal preparations in parts of South Asia. The main phytochemicals in crude aqueous and ethanolic extracts of different plant parts of Swertia chirayita collected from nine different districts of Nepal representing West, East and Central Nepal were quantified using HPLC/DAD (High Performance Liquid Chromatography-Diode Array Detection). The quantities of these phytochemicals were also compared between wild and cultivated plant parts of Swertia chirayita. Amarogentin, mangiferin, swertiamarin were the main phytochemicals in all extracts. The highest quantity of all the three phytochemicals was found in IL (inflorescence and leaf mixture) of all the collected plants samples. There was no significant difference in the amounts of these three phytochemicals between extracts from wild and cultivated plants. The result from this study substantiates the validity of cultivated Swertia chirayita for medicinal purposes and trade.Key words:  Swertia chirayita; HPLC/DAD; Wild; Cultivated; Phytochemicals; Quantification; Mangiferin; Swertiamarin; AmarogentinDOI: 10.3126/eco.v17i0.4118EcoprintAn International Journal of EcologyVol. 17, 2010Page: 59-68Uploaded date: 3 January, 2011

scholarly journals Foraging behaviour of Amegilla zonata (L.) on Ocimum kilimandscharicum Guerke

2015 ◽  
Vol 44 (1) ◽  
pp. 129-132
Author(s):  
Devinder Sharma ◽  
DP Abrol
Keyword(s):  
Environmental Factors ◽  
Medicinal Plant ◽  
Foraging Behaviour ◽  
Apis Dorsata ◽  
Jammu And Kashmir ◽  
Important Medicinal Plant

Ocimum kilimandscharicum Guerke an important medicinal plant of Jammu and Kashmir, India was found to be pollinated by insects belonging to 4 orders, 8 families and 14 species. The flowers of O. kilimandscharicum were visited abundantly by Amegilla zonata (L.) followed by Apis dorsata and A. cerana. The number of bees foraging during different times on the day ranged from 4.7 - 11.30/ 5 plants/5 min; visited 12 - 25 flowers/5 plant and spent 2.3 - 7.9 s/flower. Foraging populations of A.zonata responded significantly to environmental factors. DOI: http://dx.doi.org/10.3329/bjb.v44i1.22735 Bangladesh J. Bot. 44(1): 129-132, 2015 (March

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