scholarly journals QUANTIFICATION OF MAJOR PHYTOCHEMICALS OF SWERTIA CHIRAYITA, A MEDICINAL PLANT FROM NEPAL

1970 ◽  
Vol 17 ◽  
pp. 59-68 ◽  
Author(s):  
Susanna Phoboo ◽  
Marcia Da Silva Pinto ◽  
Prasanta C. Bhowmik ◽  
Pramod Kumar Jha ◽  
Kalidas Shetty
Keyword(s):  
Medicinal Plant ◽  
High Performance ◽  
Cultivated Plants ◽  
Swertia Chirayita ◽  
Plant Parts ◽  
Central Nepal ◽  
Significant Difference ◽  
Cultivated Plant ◽  
Array Detection ◽  
Important Medicinal Plant

Swertia chirayita is an important medicinal plant from Nepal with anti-diabetic, anti-pyretic, anti-malarial and anti-inflammatory potential and used in therapeutic herbal preparations in parts of South Asia. The main phytochemicals in crude aqueous and ethanolic extracts of different plant parts of Swertia chirayita collected from nine different districts of Nepal representing West, East and Central Nepal were quantified using HPLC/DAD (High Performance Liquid Chromatography-Diode Array Detection). The quantities of these phytochemicals were also compared between wild and cultivated plant parts of Swertia chirayita. Amarogentin, mangiferin, swertiamarin were the main phytochemicals in all extracts. The highest quantity of all the three phytochemicals was found in IL (inflorescence and leaf mixture) of all the collected plants samples. There was no significant difference in the amounts of these three phytochemicals between extracts from wild and cultivated plants. The result from this study substantiates the validity of cultivated Swertia chirayita for medicinal purposes and trade.Key words:  Swertia chirayita; HPLC/DAD; Wild; Cultivated; Phytochemicals; Quantification; Mangiferin; Swertiamarin; AmarogentinDOI: 10.3126/eco.v17i0.4118EcoprintAn International Journal of EcologyVol. 17, 2010Page: 59-68Uploaded date: 3 January, 2011

scholarly journals Trade and Sustainable Conservation of Swertia chirayita (Roxb. ex Fleming) h. Karst in Nepal

1970 ◽  
Vol 11 ◽  
pp. 125-132 ◽  
Author(s):  
Susanna Phoboo ◽  
Pramod Kumar Jha
Keyword(s):  
Medicinal Plant ◽  
Large Scale ◽  
Sustainable Use ◽  
Current Status ◽  
Fiscal Year ◽  
Sustainable Harvesting ◽  
Swertia Chirayita ◽  
Extensive Collection ◽  
Sustainable Conservation ◽  
Important Medicinal Plant

Swertia chirayita (Roxb. ex Fleming) H. Karst is one of the most important high-value medicinal plants of Nepal prized for its anti-diabetic, antimalarial, hepato-protective and anti-inflammatory properties. It is widely used as an important ingredient in medicinal preparations in Ayurveda, Unnani, Siddha, Tibetan and Chinese traditional medicine. Nepal is one of the main exporters of this important plant and the most significant importers are India and China. The large scale export of this plant has made it vulnerable in Nepal and there is an immediate need for its conservation. Detailed information on the current status of trade of this plant in Nepal is necessary to understand and make assumptions on its current market standing or future predictions of demand. S. chirayita is traded in 61 of the 75 districts of Nepal and constituted about 3% in the total medicinal plant traded in the fiscal year 2008-2009. Due to extensive collection of S. chirayita from the wild, there is a need for exploring alternative conservation options in order to preserve the remaining wild population. Sustainable harvesting and cultivation can help in its conservation. This paper briefly reviews the state of trade and sustainable conservation of this important medicinal plant in Nepal. Key words: Swertia chirayita; trade; cultivation; sustainable use; sustainable harvesting DOI: 10.3126/njst.v11i0.4134Nepal Journal of Science and Technology 11 (2010) 125-132

scholarly journals Chemical fingerprinting and quantitative analysis of two common Gleditsia sinensis fruits using HPLC-DAD

2013 ◽  
Vol 63 (4) ◽  
pp. 505-515 ◽  
Author(s):  
Jianping Chen ◽  
Zhonggui Li ◽  
Ken Y. Z. Zheng ◽  
Ava J. Y. Guo ◽  
Kevin Y. Zhu ◽  
...  
Keyword(s):  
High Performance ◽  
Developmental Stages ◽  
Diode Array ◽  
Diode Array Detection ◽  
Echinocystic Acid ◽  
Chromatographic Fingerprints ◽  
Significant Difference ◽  
Chemical Fingerprinting ◽  
Array Detection ◽  
Gleditsia Sinensis

Abstract Gleditsiae Fructus Abnormalis and Gleditsiae Sinensis Fructus are obtained from different developmental stages of fruits from Gleditsia sinensis Lam. (Leguminosae). The possible interchangeable usage of the two fruits, however, has long been very controversial. Here, high performance liquid chromatography coupled with diode array detection was developed to explore their chemical fingerprinting profiles. Besides, the amounts of aglycones of saponin compounds, echinocystic acid and oleanolic acid in both fruits were quantified. The results indicated that there was no significant difference in the content of aglycones from the two types of fruits. However, their chromatographic fingerprints showed distinct characteristics. Therefore, the interchangeable application of these fruits has to be taken with a specific precaution.

scholarly journals Sorrel (Rumex acetosa L.): Not Only a Weed but a Promising Vegetable and Medicinal Plant

2020 ◽  
Vol 86 (3-4) ◽  
pp. 234-246
Author(s):  
Helena Korpelainen ◽  
Maria Pietiläinen
Keyword(s):  
Medicinal Plant ◽  
Oxalic Acid ◽  
Bioactive Compounds ◽  
Rumex Acetosa ◽  
Good Source ◽  
Pharmacological Activities ◽  
Plant Parts ◽  
Cultivated Plant ◽  
Ancient Times ◽  
Folk Remedies

AbstractSorrel (Rumex acetosa L.) is a perennial, dioecious herb occupying a variety of habitats. Sorrel has been utilized as a wild gathered plant for thousands of years and as a cultivated plant for centuries. Primarily roots but also other tissues have been used in folk remedies since ancient times. In recent investigations, different phytochemical and pharmacological activities of Rumex have been analyzed, and many bioactive compounds have been detected. Sorrel is also a tasty plant and its leaves are a good source of macronutrients and micronutrients. However, the presence of high levels of oxalic acid reduces the bioavailability of some minerals, especially calcium, when using uncooked plant parts. Further investigations on different sorrel compounds are needed to show their true effect. It is beneficial that sorrel is easily propagated and there are no serious insect or disease problems. A few types of R. acetosa seeds are presently available commercially, including wild types and a few cultivars.

scholarly journals Purification and Identification of Flavonoids Extracted from Loranthus Eurpaeus Fruits

2016 ◽  
Vol 10 (1) ◽  
pp. 10-15
Author(s):  
Eslah S. Rajab ◽  
Khlood W. Abood ◽  
Zaynab Y. Muhammed Hassn
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Medicinal Plant ◽  
High Performance ◽  
Methanolic Extract ◽  
Mobile Systems ◽  
Total Flavonoids ◽  
Layer Chromatography ◽  
Important Medicinal Plant ◽  
Qualitative Estimation

Loranthus eurpaeusis an important medicinal plant, which contains a lot of bioactive compounds. The dried plantfruits were extracted overnight with 80% methanol by maceration using shaker incubator 25c◦. Chemicaldetection of crud plant extracts was performed. The total flavonoids was isolated from the extract using reflux,and subjected to thin layer chromatography (TLC) using different mobile systems. The purified material wasaugmented by using high performance liquid chromatography (HPLC). The aglycon moiety was extracted byethyl acetate, and then evaporated to dryness. The dried residue then redissolved in 50% ethanol. Purificationprocedures of flavonoid were fully described in this study. The aims of this study was to detect the activecompounds present in L. eurpaeus methanolic extract then quantitative and qualitative estimation of the totalflavonoids isolated from the plant fruits.

scholarly journals Protein-Based Fingerprint Analysis for the Identification of Ranae Oviductus Using RP-HPLC

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1687 ◽  
Author(s):  
Yuanshuai Gan ◽  
Yao Xiao ◽  
Shihan Wang ◽  
Hongye Guo ◽  
Min Liu ◽  
...  
Keyword(s):  
Clustering Analysis ◽  
High Performance ◽  
Principal Component ◽  
Reversed Phase ◽  
Ultrapure Water ◽  
Fingerprint Analysis ◽  
Rp Hplc ◽  
Significant Difference ◽  
Array Detection ◽  
Counterfeit Products

This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA (v/v) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779–0.980. The fingerprint chromatogram of Ranae Oviductus showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified Ranae Oviductus from the samples. These results indicated that the method established in this work was reliable.

scholarly journals The application of an Agrobacterium-mediated in planta transformation system in a Catharanthus roseus medicinal plant

2019 ◽  
Vol 56 (No. 1) ◽  
pp. 34-41
Author(s):  
Zahra Bahari ◽  
Sima Sazegari ◽  
Ali Niazi ◽  
Alireza Afsharifar
Keyword(s):  
Medicinal Plant ◽  
Catharanthus Roseus ◽  
Normal Growth ◽  
Elisa Test ◽  
Transformation Rate ◽  
In Planta ◽  
Significant Difference ◽  
Transgenic Lines ◽  
Important Medicinal Plant ◽  
Do So

The lack of an efficient protocol for the transformation of Catharanthus roseus, as an important medicinal plant, causes obstacles in the genetic engineering programmes of this plant. Therefore, the present study was conducted to examine a convenient and reliable system for generating stable transgenic lines based on the in planta method. To do so, two different in planta Agrobacterium infection systems were investigated. The transgenic lines were produced through ovary injection and shoot apical meristem (SAM) immersing methods. The presence and expression of the GFP transgene in the T0 and T1 lines were confirmed by PCR and RT-PCR. Moreover, the ELISA test confirmed the GFP expression in the transgenic lines. Apart from the superiority of the ovary injection over the SAM inoculation due to the normal growth and healthy appearance, it also showed a significant difference from the point of the confirmed transgenic plant numbers. GFP integration was confirmed in 6 out of 50 T1 plants from the ovary injection and only 3 out of 150 plants generated from the SAM inoculation. In addition to the comparison of these two separate methods, we reached a new convenient in planta system by a 12% transformation rate for generating the C. roseus transgenic lines. According to the result of this study, ovary injection can be introduced as a novel, facile, and stable method for the Catharanthus transformation programme.

scholarly journals Phyto-pharmacological and Biological Aspects of Vitex negundo Medicinal Plant - A Review

2021 ◽  
pp. 17-32
Author(s):  
B. Neha ◽  
R. Jannavi ◽  
Prabhu Sukumaran
Keyword(s):  
Medicinal Plant ◽  
Modern Medicine ◽  
Vitex Negundo ◽  
Plant Parts ◽  
Therapeutic Value ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Biological Aspects ◽  
Phytochemical Components ◽  
Important Medicinal Plant

Vitex negundo Linn is an important medicinal plant belonging to the Verbenaceae family. Every part of the plant is enriched with therapeutic value; hence the plant plays a pivotal role in traditional medicine systems. The presence of secondary metabolites such as alkaloids, flavonoids, terpenoids and phenolic compounds in the various plant parts are responsible for the anti-oxidant, anti-inflammatory, anti-microbial and anti-cancer properties which are being exploited in the treatment of cancer, cardiovascular diseases and so on. The major phytochemical components are Vitexin (8-(β-D-Glucopyranosyl)-4′,5,7-trihydroxyflavone), Isovitexin (5,7-dihydroxy-2-(4-hydroxyphenyl) 6[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl) oxan-2-yl]chromen-4-one), Vitedoin and Negundin((7R,8S)-8-(4-hydroxy-3-methoxyphenyl)-6, 7-bis(hydroxymethyl)-3-methoxy-7,8-dihydronaphthalen-2-ol). The scope of Vitex negundo as an adjuvant in modern medicine is huge. Therefore, this review focuses on research conducted till date to evaluate the phytochemical composition, and pharmacological activities of Vitex negundo medicinal plant.

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