scholarly journals Evaluation of Total Phenolic Contents and Antioxidant Activity of Carrot Callus Extracts as Affected by Phenylalanine Precursor

2016 ◽  
Vol 25 (2) ◽  
pp. 207-221 ◽  
Author(s):  
Nermeen M Arafa ◽  
Mona M Ibrahim ◽  
Usama Ibrahim Aly
Keyword(s):  
Antioxidant Activity ◽  
Daucus Carota ◽  
Light Condition ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Light Conditions ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Dark Conditions

Carrot (Daucus carota) is a valuable plant with both therapeutic and horticultural potential. Stem, petiole and root derived calli of carrot were obtained on solid MS supplemented with 1 mg/l BAP + 2 mg/l NAA. Callus cultures supplemented with different L-phenylalanine (PHE) concentrations under light and dark conditions were evaluated for their antioxidant activity and total phenolic contents. The authors showed that PHE supplementation in Daucus carota cultures was necessary to raise the extraction yield percentage. Antioxidant assays such as DPPH scavenging activity and ?-carotene bleaching have been carried out. In DPPH system, callus extracts from different explants grown under light conditions displayed lower DPPH radical scavenging activity at all PHE levels compared with that grown under dark conditions. Moreover, under both light and dark conditions callus cultures grown on MS supplemented with 1 mg/l BAP + 2 mg/l NAA plus 1000 mg/l PHE were recorded to yield the maximum values as antioxidant activities. Regarding ?-carotene bleaching assay, under light condition the callus extract of stem, root and petiole recorded an inhibition of linolic acid 47.9, 41.43 and 39%) which is lower compared with dark grown cultures, respectively (52.46, 72.71 and 73.26%). Effect of different concentrations of phenylalanine on the total phenolic content of carrot callus extract examined under light conditions varied from 0.33 to 2 mg/g DW and 0.51 to 3.69 mg/g DW under dark conditions as expressedPlant Tissue Cult. & Biotech. 25(2): 207-221, 2015 (December)

SKRINING FITOKIMIA DAN UJI AKTIVITAS ANTIOKSIDAN DAN ANTITUMOR 20 TUMBUHAN OBAT ASAL KABUPATEN MANOKWARI

2008 ◽  
Vol 7 (1) ◽  
Author(s):  
Bimo Budi Santoso
Keyword(s):  
Antioxidant Activity ◽  
Medicinal Plants ◽  
Antitumor Activity ◽  
Traditional Method ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

<strong><em>Flavanoid, steroid, and terpenoid contents of 20 Manokwari medicinal plants were analyzed.  The antioxidant activity, total phenolic contents and antitumor activity of 20 Manokwari medicinal plants were also evaluated. The result shows that 83% positif to flavanoid test, 59% positif to terpenoid test and only 25% positif steroid. Antioxidant activity and total phenolic contents evaluated using Ferric Thiocianate (FTC) and Folin-Ciocalteu methods respectively. Antioxidant activity and total phenolic contents of medicinal plants were extracted by the traditional method, boiling in water and also in 80% methanol. Twenty plants evaluated in both exstracts have significantly varies of antioxidant activities and phenolic contents,  A significant and linier correlation coefficient between the antioxidant activity and the total phenolic content was found in both aqueous (R<sup>2</sup>= 0,77) and methanol (R<sup>2</sup> = 0,85). Antitumor activity was tested using cell maurine P-388 and only 2 of medicinal plants are active to inhibit cell maurine P-388. Comparing extraction efficiency of the two methods, the methanol extracted phenolic compounds more efficiently, and antioxidant activity of the extract was higher.</em></strong>

scholarly journals Loranthus pulverulentus: A Potent Source of Natural Antioxidants and Alternative Medicine

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Muhammad Asam Raza ◽  
Rukhsana Kausar ◽  
Faraz Ali Rana ◽  
Muhammad Danish ◽  
Durre Shahwar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Natural Antioxidants ◽  
Stem Bark ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Strong Correlations ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Total Antioxidant

This study was designed to evaluate the antioxidant potential ofLoranthus pulverulentus. Stem bark, leaves, and seeds ofLoranthus pulverulentuswere extracted in methanol:water (90 : 10) and partitioned with n-hexane, chloroform, ethyl acetate, and n-butanol successively using partition chromatography. Total phenolic contents and antioxidant potential were checked using standard protocols. Total phenolic contents of all extracts were determined, using Folin–Ciocalteu reagent, and ranged between 151 ± 2.1 and 396 ± 1.6 for stem bark, 137 ± 0.9 and 430 ± 2.2 for, and 39 ± 0.6 and 231 ± 1.7 for seeds. The antioxidant potential of extracts was evaluated; namely, DPPH, FRAP, and total antioxidant models. The ethyl acetate extract of stem-bark, leaves, and seeds showed the highest activity in DPPH (94.5 ± 2.1%, 96.30 ± 0.9%, and 92.30 ± 1.1%, IC5015.9 ± 0.5 μg, 14.5 ± 0.8, and 102.7 ± 1.3, resp.), FRAP (7.7 ± 0.6, 7.5 ± 0.7 and 6.6 ± 0.7, resp.), and total antioxidant (0.95 ± 0.09, 1.19 ± 0.09, and 0.686 ± 0.08, resp.). Strong correlations were observed between total phenols versus total antioxidant activity, DPPH, and FRAP withR2values ranging from 0.8185 to 0.9951 (stem-bark), 0.6728 to 0.8648 (leaves), and 0.8658 to 0.9910 (seed) which indicated that phenolic contents are the major constituents responsible for antioxidant activity.

Quantitative Analysis of Total Phenolic Contents and Antioxidant Activity of Fresh Field Natural Rubber Latex

2015 ◽  
Vol 354 (1) ◽  
pp. 265-272 ◽  
Author(s):  
Suwimon Siriwong ◽  
Adisai Rungvichaniwat ◽  
Pairote Klinpituksa ◽  
Khalid Hamid Musa ◽  
Aminah Abdullah
Keyword(s):  
Antioxidant Activity ◽  
Quantitative Analysis ◽  
Natural Rubber ◽  
Natural Rubber Latex ◽  
Total Phenolic ◽  
Rubber Latex ◽  
Phenolic Contents ◽  
Total Phenolic Contents

scholarly journals Abts cation scavenging activity and total phenolic content of three moss species

2012 ◽  
Vol 66 (5) ◽  
pp. 723-726 ◽  
Author(s):  
Boris Pejin ◽  
Jelena Bogdanovic-Pristov
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Moss Species ◽  
Phenolic Contents ◽  
Antioxidant Agents ◽  
Acid Cation ◽  
First Time

A limited number of mosses have ever been studied in regard to antioxidant activity; with only few belonging to the European species. As part of our on-going screening of bryophytes as natural antioxidant resources, a relevant biological activity investigation was conducted on the three moss species, namely Brachythecium rutabulum (Hedw.) Schimp. (Brachytheciaceae), Calliergonella cuspidata (Hedw.) Loeske (Hypnaceae) and Hypnum mammillatum (Brid.) Loeske (Hypnaceae), collected in Germany. The antioxidant activity of corresponding lyophilised aqueous extracts (teas) was evaluated on the basis of their ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)] cation scavenging activities. The total phenolic contents were determined as well. This study led to the detection of a moderate total phenolic content of the moss B. rutabulum, indicating for the first time its potential in searching for novel antioxidant agents.

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