scholarly journals Status and monitoring of the buff-tailed bumblebee Bombus terrestris Linnaeus (Hymenoptera: Apidae) in Southern Finland

2019 ◽  
Vol 25 (2) ◽  
pp. 49-56 ◽  
Author(s):  
Salla-Riikka Vesterlund ◽  
Maria Kakko ◽  
Anti Vasemägi ◽  
Jouni Sorvari
Keyword(s):  
Risk Assessment ◽  
Species Identification ◽  
Native Plants ◽  
Bombus Terrestris ◽  
Sensu Stricto ◽  
Degraded Dna ◽  
Native Bees ◽  
Regular Monitoring ◽  
Pcr Rflp ◽  
Correct Species Identification

Bombus terrestris can cause pollination disturbance in native plants and compete with native bumblebees and other pollinators. The accompanying non-native parasites may also threaten native bees. We report new observations of the commercially used Bombus terrestris (Linnaeus, 1758) using trapping data and sporadic samples identified with a PCR–RFLP-method for degraded DNA. A total of 863 individuals (355 queens, 442 workers, 66 drones) of Bombus sensu stricto were collected during the years 2008–9, of which, 642 were B. lucorum, ten B. cryptarum, four B. terrestris and none were B. magnus. Three trap types were compared in two modified transects near areas that use the commercial B. terrestris for pollination in Southern Finland: the tree trap that was hung at approximately 3 metres height was the most effective. Regular monitoring is important in the risk assessment of B. terrestris, and for correct species identification, molecular methods are recommended.

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

2011 ◽  
Vol 14 (2) ◽  
pp. 285-286 ◽  
Author(s):  
J. Karakulska ◽  
A. Pobucewicz ◽  
P. Nawrotek ◽  
M. Muszyńska ◽  
A. Furowicz ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Species Identification ◽  
Genetic Relationship ◽  
Molecular Typing ◽  
Rapd Analysis ◽  
Milk Samples ◽  
Rapd Pcr ◽  
Pcr Rflp ◽  
Mastitic Milk ◽  
Coa Gene

Molecular typing ofStaphylococcus aureusbased on PCR-RFLP ofcoagene and RAPD analysisThe aim of this study was molecular identification ofS. aureusstrains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains thegapgene (930 bp) was amplified, which enabled their affiliation to theStaphylococcusgenus to be established. PCR-RFLP withAluI endonuclease of thegapgene as well asnuc(450 bp) andcoa(1130 bp) gene amplification allowed preciseS. aureusspecies identification. One hundred percent of the genetic relationship between strains was establishedviaRAPD-PCR and coa-typing.

scholarly journals REP-PCR analisis of single agent of cucumber bacterial diseases

2017 ◽  
Vol 15 (1) ◽  
pp. 25-31
Author(s):  
L. A. Dankevych
Keyword(s):  
Species Identification ◽  
Statistical Methods ◽  
Genetic Heterogeneity ◽  
Significant Relationship ◽  
Molecular Genetic ◽  
Single Agent ◽  
Genetic Homogeneity ◽  
Bacterial Diseases ◽  
Typical Strain ◽  
Correct Species Identification

Aim. For the purpose of correct species identification and estimation of population’s heterogeneity, the fingerprinting of the genome of isolated by us Pectobacterium sp., collection «Erwinia toxica» strains and typical representatives of certain species of Pectobacterium and Diskeya genera has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR), mathematical-statistical methods of research were used. Results. On the basic of BOX, REP and ERIC profiles the significant affinity between isolated Pectobacterium sp. and collections «Erwinia toxica» strains with the typical P. carotovorum susp. carotovorum UCM B1075T has been established. Genetic heterogeneity of isolated Pectobacterium sp. and collections «Erwinia toxica» strains has been estimated. Conclusions. It has been found the significant relationship between isolates Pectobacterium sp. and the collection «Erwinia toxica» strains with the typical strain P. carotovorum susp carotovorum UCM B1075T on the basic of their BOX, REP and ERIC profiles. Most likely, this indicates that they belong to this species. The genetic homogeneity of isolated Pectobacterium sp. strains of and the genetic heterogeneity of the collection «Erwinia toxica» strains is probably due to the plant’s selection from similar or different region.Keywords: identification, genetic heterogeneity, REPPCR, «Erwinia toxica», Pectobacterium sp.

scholarly journals Species Identification of Five Penaeid Shrimps Using PCR-RFLP and SSCP Analyses of 16S Ribosomal DNA

BMB Reports ◽  
2005 ◽  
Vol 38 (4) ◽  
pp. 491-499 ◽  
Author(s):  
Bavornlak Khamnamtong ◽  
Sirawut Klinbunga ◽  
Piamsak Menasveta
Keyword(s):  
Species Identification ◽  
Ribosomal Dna ◽  
16S Ribosomal Dna ◽  
Pcr Rflp ◽  
Penaeid Shrimps

scholarly journals A PCR Method That Can Be Further Developed into PCR-RFLP Assay for Eight Animal Species Identification

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Feng Guan ◽  
Yu-Ting Jin ◽  
Jin Zhao ◽  
Ai-Chun Xu ◽  
Yuan-Yuan Luo
Keyword(s):  
Species Identification ◽  
Animal Species ◽  
Limit Of Detection ◽  
Cost Effective ◽  
Universal Primers ◽  
Universal Primer ◽  
Routine Identification ◽  
Pcr Rflp ◽  
Fast Processing ◽  
Rflp Assay

There are many PCR-based methods for animal species identification; however, their detection numbers are limited or could not identify unknown species. We set out to solve this problem by developing a universal primer PCR assay for simultaneous identification of eight animal species, including goat, sheep, deer, buffalo, cattle, yak, pig, and camel. In this assay, the variable lengths of mitochondrial DNA were amplified using a pair of universal primers. PCR amplifications yielded 760 bp, 737 bp, 537 bp, 486 bp, 481 bp, 464 bp, 429 bp, and 359 bp length fragments for goat, sheep, deer, buffalo, cattle, yak, pig, and camel, respectively. This primer pair had no cross-reaction with other common domestic animals and fish. The limit of detection varied from 0.01 to 0.05 ng of genomic DNA for eight animal species in a 20 µl PCR mixture. Each PCR product could be further digested into fragments with variable sizes and qualitative analysis by SspI restriction enzyme. This developed PCR-RFLP assay was sufficient to distinguish all targeted species. Compared with the previous published related methods, this approach is simple, with high throughput, fast processing rates, and more cost-effective for routine identification of meat in foodstuffs.

Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance

2010 ◽  
Vol 56 (4) ◽  
pp. 348-351 ◽  
Author(s):  
Eszter Deak ◽  
Kizee A. Etienne ◽  
Shawn R. Lockhart ◽  
Lalitha Gade ◽  
Tom Chiller ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Species Identification ◽  
Population Based ◽  
Rapid Identification ◽  
Attractive Alternative ◽  
Candida Sp ◽  
Luminex Assay ◽  
Combination Of Methods ◽  
User Friendly ◽  
Correct Species Identification

A Candida -specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.

scholarly journals Molecular Characterization of the Fusarium graminearum Species Complex in Japan

2008 ◽  
Vol 98 (2) ◽  
pp. 159-166 ◽  
Author(s):  
H. Suga ◽  
G. W. Karugia ◽  
T. Ward ◽  
L. R. Gale ◽  
K. Tomimura ◽  
...  
Keyword(s):  
Fusarium Graminearum ◽  
Species Complex ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
Sensu Stricto ◽  
Rflp Markers ◽  
Differential Distribution ◽  
The North ◽  
Pcr Rflp ◽  
Fusarium Graminearum Species Complex

Members of the Fusarium graminearum species complex are important cereal pathogens worldwide and belong to one of at least nine phylogenetically distinct species. We examined 298 strains of the F. graminearum species complex collected from wheat or barley in Japan to determine the species and trichothecene chemotype. Phylogenetic analyses and species-diagnostic polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLPs) revealed the presence and differential distribution of F. graminearum sensu stricto (s. str.) and F. asiaticum in Japan. F. graminearum s. str. is predominant in the north, especially in the Hokkaido area, while F. asiaticum is predominant in southern regions. In the Tohoku area, these species co-occurred. Trichothecene chemotyping of all strains by multiplex PCR revealed significantly different chemotype compositions of these species. All 50 strains of F. graminearum s. str. were of a 15- or 3-acetyl deoxynivalenol type, while 173 (70%) out of 246 strains of F. asiaticum were of a nivalenol type. The possibility of gene flow between the two species was investigated by use of 15 PCR-RFLP markers developed in this study. However, no obvious hybrids were detected from 98 strains examined, including strains collected from regions where both species co-occur.

scholarly journals New species within Candida parapsilosis and Candida glabrata

2019 ◽  
pp. 51-57
Author(s):  
Dariusz Domański ◽  
Magdalena Anna Sikora ◽  
Robert Tomasz Kuthan ◽  
Ewa Augustynowicz-Kopeć ◽  
Ewa Swoboda-Kopeć
Keyword(s):  
Candida Glabrata ◽  
Candida Parapsilosis ◽  
Secondary Alcohol ◽  
Sensu Stricto ◽  
Diagnostic Methods ◽  
D2 Domain ◽  
Dehydrogenase Gene ◽  
Pcr Rflp ◽  
Secondary Alcohol Dehydrogenase ◽  
Candida Nivariensis

Introduction: Candida parapsilosis and Candida glabrata are another yeasts that form complexes of crypospecies. Although these species have been described more than a decade ago, knowledge about them is still limited. The reason for this is the large phenotypic similarity that unables them from being differentiated by classical diagnostic methods. The aim of the study was to identify species of clinical strains within C. glabrata and C. parapsilosis complexes. Material and methods: Standard PCR-RFLP of the secondary alcohol dehydrogenase gene (SADH) with BanI restriction enzyme served to determine species affiliation within the C. parapsilosis complex. The internal transcribed spacer was used to confirm the identification of C. glabrata sensu stricto. The D1/D2 domain of the 26S rDNA gene was sequenced in order to identify C. nivariensis and C. bracarensis strains. Results: As a result of the molecular analysis, 24 Candida nivariensis isolates and 4 C. metapsilosis strains and 9 C. orthopsilosis strains were detected. Conclusions: Prevalence of new cryptic species was relatively low.

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