REP-PCR analisis of single agent of cucumber bacterial diseases

Aim. For the purpose of correct species identification and estimation of population’s heterogeneity, the fingerprinting of the genome of isolated by us Pectobacterium sp., collection «Erwinia toxica» strains and typical representatives of certain species of Pectobacterium and Diskeya genera has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR), mathematical-statistical methods of research were used. Results. On the basic of BOX, REP and ERIC profiles the significant affinity between isolated Pectobacterium sp. and collections «Erwinia toxica» strains with the typical P. carotovorum susp. carotovorum UCM B1075T has been established. Genetic heterogeneity of isolated Pectobacterium sp. and collections «Erwinia toxica» strains has been estimated. Conclusions. It has been found the significant relationship between isolates Pectobacterium sp. and the collection «Erwinia toxica» strains with the typical strain P. carotovorum susp carotovorum UCM B1075T on the basic of their BOX, REP and ERIC profiles. Most likely, this indicates that they belong to this species. The genetic homogeneity of isolated Pectobacterium sp. strains of and the genetic heterogeneity of the collection «Erwinia toxica» strains is probably due to the plant’s selection from similar or different region.Keywords: identification, genetic heterogeneity, REPPCR, «Erwinia toxica», Pectobacterium sp.

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Genetic profiling of bacteria belongs to genus Pseudomonas, what affects legumes

Faktori eksperimental'noi evolucii organizmiv ◽

10.7124/feeo.v22.935 ◽

2018 ◽

Vol 22 ◽

pp. 120-125

Author(s):

L. A. Dankevich

Keyword(s):

Species Identification ◽

Pseudomonas Syringae ◽

Causative Agent ◽

Genetic Heterogeneity ◽

Molecular Phylogenetics ◽

Molecular Genetic ◽

Close Similarity ◽

Genetic Profiling ◽

Typical Representative

Aim. For the purpose of correct inter species identification and estimation of group’s heterogeneity, the genome fingerprinting of isolated by us and collection "Pseudomonas lupini" strains as well as typical representative of genus, affecting legumes, has been carried out. Methods. In the course of research, microbiological, molecular genetic (REP-PCR) methods and method of molecular phylogenetics (UPGMA) were used. Results. The genetic heterogeneity of isolated and collections "Pseudomonas lupini" strains has been estimated. A relationship between isolated and collections "Pseudomonas lupini" strains with the typical Pseudomonas syringae strains, affecting legumes, for BOX, REP and ERIC profiles has been determined. Conclusions. BOX, ERIC and REP-profiling of the genome of the agent of lupines’ brown spottiness revealed significant genetic heterogeneity of its population (from 20 to 50 % of heterogeneity) and close similarity of this pathogen to representatives of the species Pseudomonas syringae.Keywords: identification, genetic heterogeneity, REP-PCR, causative agent of lupines’ brown spottiness.

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Utility of a Luminex-based assay for multiplexed, rapid species identification of Candida isolates from an ongoing candidemia surveillance

Canadian Journal of Microbiology ◽

10.1139/w10-003 ◽

2010 ◽

Vol 56 (4) ◽

pp. 348-351 ◽

Cited By ~ 23

Author(s):

Eszter Deak ◽

Kizee A. Etienne ◽

Shawn R. Lockhart ◽

Lalitha Gade ◽

Tom Chiller ◽

...

Keyword(s):

Sequence Analysis ◽

Species Identification ◽

Population Based ◽

Rapid Identification ◽

Attractive Alternative ◽

Candida Sp ◽

Luminex Assay ◽

Combination Of Methods ◽

User Friendly ◽

Correct Species Identification

A Candida -specific Luminex-based assay with 11 probes was employed for multiplexed, rapid identification of 1182 Candida sp. isolates that were received as part of an ongoing population-based surveillance. All the Candida isolates were previously identified by a combination of methods, including phenotype and sequence analysis. Results showed that the Luminex assay was an attractive alternative to reference methods, as it is rapid, yields correct species identification, and is user friendly.

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An analysis of morphological and molecular genetic characters for species identification of amber snails Succinea putris (Succineidae)

Invertzool ◽

10.15298/invertzool.17.1.01 ◽

2020 ◽

Vol 17 (1) ◽

pp. 1-17

Author(s):

E. E. Prokhorova ◽

R. R. Usmanova ◽

G. L. Ataev

Keyword(s):

Species Identification ◽

Molecular Genetic ◽

Genetic Characters

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PENGARUH KETERAMPILAN TERHADAP PRODUKTIVITAS KERJA KARYAWAN PADA KONVEKSI ISTANA MODE MADIUN

EQUILIBRIUM Jurnal Ilmiah Ekonomi dan Pembelajarannya ◽

10.25273/equilibrium.v4i1.647 ◽

2016 ◽

Vol 4 (1) ◽

pp. 96 ◽

Cited By ~ 1

Author(s):

Wiwin Wiranti

Keyword(s):

Regression Analysis ◽

Data Collection ◽

Statistical Methods ◽

Significant Relationship ◽

Work Productivity ◽

Sampling Technique ◽

T Test ◽

Employee Productivity ◽

Test Results ◽

F Test

<p>This study aims to determine tbe Employee Skills in the Konveksi Istana Mode Madiun, to determine Employee Productivity in the Konveksi Istana Mode Madiun, as well as to determine the presence or absence of Vocational Effect on Productivity Among Employees at the Konveksi Istana Mode Madiun. The samples in this study is purposive sampling technique that all employees at the Konveksi Istana Mode Madiun part of convection, amounting to 30 people. Data collection using questionnaires, documentation and interviews.In analyzing the data using statistical methods Product Moment, To test this hypothesis using the r test, F test and t test. The results of the regression analysis ofY = a +bX, the result Y = 11,737 + 0,757X, to test that rcount > r liable (0.784 > 0.361), or may be rejected HO means that there is a skills significant relationship with work productivity of employee at the Konveksi Istana Mode Madiun. Furthermore, also obtained results which indicate that the F test ofF count >F table (44,5752::4.196) or ::;;Sighit Sigprob (0,000 ::;;0.05). Thus HO is rejected, it means there is an overall effect of skills on labor productivity at the Konveksi Istana Mode Madiun. In addition, the t-test results obtained thit> ttab (6.676 > 2.048) or Sig hit < Sig prob (0.000 < 0.025) this situation can be said to be no different from the effect of employee skills on work productivity of employee at the Konveksi Istana Mode Madiun.</p>

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First Record of Trichobilharzia physellae (Talbot, 1936) in Europe, a Possible Causative Agent of Cercarial Dermatitis

Pathogens ◽

10.3390/pathogens10111473 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1473

Author(s):

Nikolaus Helmer ◽

Hubert Blatterer ◽

Christoph Hörweg ◽

Susanne Reier ◽

Helmut Sattmann ◽

...

Keyword(s):

Species Identification ◽

Molecular Genetic ◽

First Record ◽

Integrative Taxonomy ◽

Freshwater Snails ◽

Cercarial Dermatitis ◽

Close Relationship ◽

Avian Schistosomes ◽

First Time

Several species of avian schistosomes are known to cause dermatitis in humans worldwide. In Europe, this applies above all to species of the genus Trichobilharzia. For Austria, a lot of data are available on cercarial dermatitis and on the occurrence of Trichobilharzia, yet species identification of trematodes in most cases is doubtful due to the challenging morphological determination of cercariae. During a survey of trematodes in freshwater snails, we were able to detect a species in the snail Physella acuta (Draparnaud, 1805) hitherto unknown for Austria, Trichobilharzia physellae; this is also the first time this species has been reported in Europe. Species identification was performed by integrative taxonomy combining morphological investigations with molecular genetic analyses. The results show a very close relationship between the parasite found in Austria and North American specimens (similarity found in CO1 ≥99.57%). Therefore, a recent introduction of T. physellae into Europe can be assumed.

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A Study of Accountant Required Skills to Have Efficient Participation in the Implementation of XBRL

The Winners ◽

10.21512/tw.v13i1.664 ◽

2012 ◽

Vol 13 (1) ◽

pp. 20

Author(s):

Heshmatolah Asadizeidabadi

Keyword(s):

Statistical Methods ◽

Significant Relationship ◽

Pearson Correlation ◽

Maintenance Phase ◽

Business Environment ◽

Successful Implementation ◽

Business Reporting ◽

The World ◽

Analyze Data

The role of the accountants in a business environment has been evolving over the years. Accountants need to have the implementation and maintenance skills of XBRL (extensible business reporting language) which is revolutionizing business reporting around the world, in the organization. The present study encompasses two aspects. First, it addresses the role of the accountant during the implementation and maintenance phase. Second, it finds the characteristics and skills of accountants that can help them in a successful implementation and maintenance of XBRL. In order to collect the data a questionnaire has been used. To analyze data two statistical methods, Regression and Pearson correlation have been used. The results of the study confirm that there is a significant relationship between the participation of accountants and successful implementation of XBRL.

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Mycobacterium Species Identification and Rifampin Resistance Testing with High-Density DNA Probe Arrays

Journal of Clinical Microbiology ◽

10.1128/jcm.37.1.49-55.1999 ◽

1999 ◽

Vol 37 (1) ◽

pp. 49-55 ◽

Cited By ~ 171

Author(s):

A. Troesch ◽

H. Nguyen ◽

C. G. Miyada ◽

S. Desvarenne ◽

T. R. Gingeras ◽

...

Keyword(s):

Species Identification ◽

Susceptibility Testing ◽

Genetic Material ◽

Dna Probe ◽

Resistance Testing ◽

Bacterial Diseases ◽

Parallel Testing ◽

Assay Time ◽

Rifampin Resistance ◽

Rrna Sequences

Species identification within the genus Mycobacteriumand subsequent antibiotic susceptibility testing still rely on time-consuming, culture-based methods. Despite the recent development of DNA probes, which greatly reduce assay time, there is a need for a single platform assay capable of answering the multitude of diagnostic questions associated with this genus. We describe the use of a DNA probe array based on two sequence databases: one for the species identification of mycobacteria (82 unique 16S rRNA sequences corresponding to 54 phenotypical species) and the other for detectingMycobacterium tuberculosis rifampin resistance (rpoB alleles). Species identification or rifampin resistance was determined by hybridizing fluorescently labeled, amplified genetic material generated from bacterial colonies to the array. Seventy mycobacterial isolates from 27 different species and 15 rifampin-resistant M. tuberculosis strains were tested. A total of 26 of 27 species were correctly identified as well as all of the rpoB mutants. This parallel testing format opens new perspectives in terms of patient management for bacterial diseases by allowing a number of genetic tests to be simultaneously run.

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The current approaches to the study of algae: DNA barcoding and DNA taxonomy

Issues of modern algology (Вопросы современной альгологии) ◽

10.33624/2311-0147-2021-2(26)-124-130 ◽

2021 ◽

pp. 124-130

Author(s):

Anna D. Temraleeva ◽

Elena S. Krivina ◽

Yury S. Bukin

Keyword(s):

Phylogenetic Analysis ◽

Dna Sequencing ◽

Dna Barcoding ◽

Species Identification ◽

Green Algae ◽

Molecular Genetic ◽

Algal Species ◽

Species Boundaries ◽

Sequencing Technology ◽

Dna Taxonomy

The understanding of the impossibility of distinguishing algal species based on morphological features came with the development of DNA sequencing technology, which today is a necessary tool for defining species boundaries and testing traditional species concepts. The paper discusses popular approaches to species identification (DNA barcoding) and the description of new and revision of known species (DNA taxonomy) using molecular genetic methods. The requirements and limitations in their work are given, as well as examples of phylogenetic analysis of green algae from the clade Moewusinia and Parachlorella, including the genus Micractinium.

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