scholarly journals Basics of the Development of Microwave Intensification of Upstream on the Example of Escherichia coli

2021 ◽  
Vol 10 (4) ◽  
pp. 72-80
Author(s):  
D. B. Kuznetsov ◽  
A. Yu. Mironov ◽  
V. A. Neschislyaev ◽  
I. L. Volkhin ◽  
A. M. Korolyuk ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Microwave Radiation ◽  
Coli Strain ◽  
E Coli ◽  
Optimal Processing ◽  
Lux Operon ◽  
Adsorption Of Water ◽  
New Ideas ◽  
Culture Growth ◽  
Number Of Publications

Introduction. E. coli strains are the main microorganisms used for the production of a number of important biopharmaceutical products. There are no natural sources of microwave radiation on Earth, as it is absorbed by the upper atmosphere. No one doubts the importance of studying the biological effect of microwave radiation. The number of publications devoted to this problem is growing every year, and new ideas for the use of microwaves in drug production technology are emerging.Aim. Reveal the main effects of microwave irradiation and develop a technology for microwave intensification of E. coli culture growth.Materials and methods. This study presents the results of atomic force microscopy, refractometry, NMR relaxometry, turbidimetry, and lumimetry, demonstrating the possibility of microwave intensification of the cultivation process.Results and discussion. It was found that microwave irradiation leads to changes in the mobility of protons and the adsorption of water molecules on biopolymers and cells. These are the main links in the mechanism of "non-thermal" microwave action. A single microwave irradiation, depending on a number of parameters, can decrease or increase the growth of biomass. Studies of the bioluminescence of the E. coli strain with the lux-operon have shown that the optimal processing conditions do not negatively affect the luciferase production and metabolic activity of cells. Conclusion. The intensification procedure using microwave radiation can be considered a promising method and can provide new ideas for various applications in biotechnology.

Transport of escherichia coli through soil to groundwater traced by randomly amplified polymorphic DNA (RAPD)

1997 ◽  
Vol 35 (11-12) ◽  
pp. 351-357 ◽  
Author(s):  
R. Rothmaier ◽  
A. Weidenmann ◽  
K. Botzenhart
Keyword(s):  
Negative Impact ◽  
Random Amplified Polymorphic Dna ◽  
Coli Strain ◽  
Soil Samples ◽  
Test Field ◽  
Liquid Manure ◽  
E Coli ◽  
Rapd Pattern ◽  
Geological Situation ◽  
Different Strains

Isolates (50) of E. coli obtained from liquid manure (20 bovine, 20 porcine) were genotyped using random amplified polymorphic DNA (RAPD). Typing revealed 9 and 14 different strains in bovine and porcine liquid manure respectively with no strains in common. One porcine strain, showing a simple RAPD pattern, was subcultured and spread on a test field (1.5l/m2 at 1010 cfu/l) in a drinking water protection zone with loamy to sandy sediments in the Donauried area, Baden-Wurttemberg. Soil samples and groundwaters were collected at monthly intervals October 1994 – June 1995 during which 114 E. coli isolates were recovered. The first occurrence and maximum concentration of E. coli in soil samples taken from more than 20cm depth was in January 1995, declining rapidly with depth and time. All isolates from soil and only one from groundwater showed the RAPD pattern of the spread E. coli strain. The results could not demonstrate a severe negative impact of the spreading of liquid manure on the bacteriological quality of the groundwater in the given geological situation. The distinct strain patterns found in different kinds of liquid manure suggest that genotyping of E. coli by RAPD may be an adequate tool for tracing sources of faecal contamination.

scholarly journals Regulation of Expression of the TIR-Containing Protein C Gene of the Uropathogenic Escherichia coli Strain CFT073

Pathogens ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 549
Author(s):  
Julia Ittensohn ◽  
Jacqueline Hemberger ◽  
Hannah Griffiths ◽  
Maren Keller ◽  
Simone Albrecht ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Protein C ◽  
Interleukin 1 ◽  
Coli Strain ◽  
Uropathogenic Escherichia Coli ◽  
Reporter Construct ◽  
Regulation Of Expression ◽  
E Coli ◽  
Strain Cft073 ◽  
Myeloid Differentiation Factor

The uropathogenic Escherichia coli strain CFT073 causes kidney abscesses in mice Toll/interleukin-1 receptor domain-containing protein C (TcpC) dependently and the corresponding gene is present in around 40% of E. coli isolates of pyelonephritis patients. It impairs the Toll-like receptor (TLR) signaling chain and the NACHT leucin-rich repeat PYD protein 3 inflammasome (NLRP3) by binding to TLR4 and myeloid differentiation factor 88 as well as to NLRP3 and caspase-1, respectively. Overexpression of the tcpC gene stopped replication of CFT073. Overexpression of several tcpC-truncation constructs revealed a transmembrane region, while its TIR domain induced filamentous bacteria. Based on these observations, we hypothesized that tcpC expression is presumably tightly controlled. We tested two putative promoters designated P1 and P2 located at 5′ of the gene c2397 and 5′ of the tcpC gene (c2398), respectively, which may form an operon. High pH and increasing glucose concentrations stimulated a P2 reporter construct that was considerably stronger than a P1 reporter construct, while increasing FeSO4 concentrations suppressed their activity. Human urine activated P2, demonstrating that tcpC might be induced in the urinary tract of infected patients. We conclude that P2, consisting of a 240 bp region 5′ of the tcpC gene, represents the major regulator of tcpC expression.

scholarly journals Novel Bacterial Production of Two Different Bioactive Forms of Human Stem-Cell Factor

2021 ◽  
Vol 22 (12) ◽  
pp. 6361
Author(s):  
Eunyoung Lee ◽  
Michelle Novais de Paula ◽  
Sangki Baek ◽  
Huynh Kim Khanh Ta ◽  
Minh Tan Nguyen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Ion Exchange ◽  
Stem Cell Factor ◽  
Transmembrane Domain ◽  
Coli Strain ◽  
Exchange Chromatography ◽  
Soluble Form ◽  
Human Stem Cell ◽  
E Coli ◽  
Human Stem Cell Factor

Human stem-cell factor (hSCF) stimulates the survival, proliferation, and differentiation of hematopoietic cells by binding to the c-Kit receptor. Various applications of hSCF require the efficient and reliable production of hSCF. hSCF exists in three forms: as two membrane-spanning proteins hSCF248 and hSCF229 and truncated soluble N-terminal protein hSCF164. hSCF164 is known to be insoluble when expressed in Escherichia coli cytoplasm, requiring a complex refolding procedure. The activity of hSCF248 has never been studied. Here, we investigated novel production methods for recombinant hSCF164 and hSCF248 without the refolding process. To increase the solubility of hSCF164, maltose-binding protein (MBP) and protein disulfide isomerase b’a’ domain (PDIb’a’) tags were attached to the N-terminus of hSCF164. These fusion proteins were overexpressed in soluble form in the Origami 2(DE3) E. coli strain. These solubilization effects were enhanced at a low temperature. His-hSCF248, the poly-His tagged form of hSCF248, was expressed in a highly soluble form without a solubilization tag protein, which was unexpected because His-hSCF248 contains a transmembrane domain. hSCF164 was purified using affinity and ion-exchange chromatography, and His-hSCF248 was purified by ion-exchange and gel filtration chromatography. The purified proteins stimulated the proliferation of TF-1 cells. Interestingly, the EC50 value of His-hSCF248 was 1 pg/mL, 100-fold lower than 9 ng/mL hSCF164. Additionally, His-hSCF248 decreased the doubling time, increased the proportion of S and G2/M stages in the cell cycle, and increased the c-Myc expression at a 1000-fold lower concentration than hSCF164. In conclusion, His-hSCF248 was expressed in a soluble form in E. coli and had stronger activity than hSCF164. The molecular chaperone, MBP, enabled the soluble overexpression of hSCF164.

Urban wastewater disinfection for agricultural reuse: effect of solar driven AOPs in the inactivation of a multidrug resistant E. coli strain

2015 ◽  
Vol 178 ◽  
pp. 65-73 ◽  
Author(s):  
Giovanna Ferro ◽  
Antonino Fiorentino ◽  
María Castro Alferez ◽  
M. Inmaculada Polo-López ◽  
Luigi Rizzo ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Coli Strain ◽  
Urban Wastewater ◽  
E Coli ◽  
Wastewater Disinfection

scholarly journals High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production

2013 ◽  
Vol 97 (9) ◽  
pp. 3893-3900 ◽  
Author(s):  
Odile Francesca Restaino ◽  
Ujjwal Bhaskar ◽  
Priscilla Paul ◽  
Lingyun Li ◽  
Mario De Rosa ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Coli Strain ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
E Coli ◽  
Bioengineered Heparin ◽  
Key Enzyme

scholarly journals Complementation of a metK-deficient E. coli strain with heterologous AdoMet synthetase genes

Microbiology ◽  
2017 ◽  
Vol 163 (12) ◽  
pp. 1812-1821
Author(s):  
Gwenn G. Parungao ◽  
Mojun Zhao ◽  
Qinzhe Wang ◽  
Stephen P. Zano ◽  
Ronald E. Viola ◽  
...  
Keyword(s):  
Coli Strain ◽  
E Coli ◽  
Adomet Synthetase

Advanced wastewater disinfection technologies: short and long term efficiency

1998 ◽  
Vol 38 (12) ◽  
pp. 109-117 ◽  
Author(s):  
V. Lazarova ◽  
M. L. Janex ◽  
L. Fiksdal ◽  
C. Oberg ◽  
I. Barcina ◽  
...  
Keyword(s):  
Peracetic Acid ◽  
Contact Time ◽  
Pilot Scale ◽  
Coli Strain ◽  
Plate Count ◽  
Galactosidase Activity ◽  
Virus Removal ◽  
E Coli ◽  
Bacteriophage Ms2 ◽  
Short And Long Term

Advanced disinfection processes (peracetic acid, UV irradiation and ozonation) have been tested and evaluated through bench and pilot scale studies. 3 log removals of total coliforms, faecal coliforms and faecal streptococci were achieved by 10mg/L peracetic acid at a 10min contact time, by UV radiation at 35mW.s/cm2 and by ozone at 5mg/L for 10min contact time. Higher doses are required for virus removal by UV and PAA and especially for highly resistant viruses such as F-specific bacteriophage MS2. Ozonation has the advantage of having a strong effect on all types of bacteriophages and protozoa cysts even when low treatment doses and short contact times are applied. The results of this study demonstrated that evaluation of disinfection efficiency of ozone, UV and PAA depends on the criteria and methods employed. Standard method (plate count) results showed an important disinfection effect on culturability, while results from non-standard methods (respiratory activity and β-galactosidase activity assay) indicated less reduction of viable cells. Moreover, the results confirm that disinfectants act on bacteria in different ways. It has been clearly demonstrated that b-galactosidase activity is affected by PAA while UV treatment has no or very limited effect on the enzyme activity. Even without sunlight reactivation, bacterial regrowth in seawater was observed after disinfection of sewage effluents. This study also shows that the biodegradability of sewage effluent for an E coli strain was affected differently by the oxidative disinfectants ozone and PAA. Biodegradability should therefore be considered when evaluating the total disinfection efficiency.

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