Advanced wastewater disinfection technologies: short and long term efficiency

1998 ◽  
Vol 38 (12) ◽  
pp. 109-117 ◽  
Author(s):  
V. Lazarova ◽  
M. L. Janex ◽  
L. Fiksdal ◽  
C. Oberg ◽  
I. Barcina ◽  
...  
Keyword(s):  
Peracetic Acid ◽  
Contact Time ◽  
Pilot Scale ◽  
Coli Strain ◽  
Plate Count ◽  
Galactosidase Activity ◽  
Virus Removal ◽  
E Coli ◽  
Bacteriophage Ms2 ◽  
Short And Long Term

Advanced disinfection processes (peracetic acid, UV irradiation and ozonation) have been tested and evaluated through bench and pilot scale studies. 3 log removals of total coliforms, faecal coliforms and faecal streptococci were achieved by 10mg/L peracetic acid at a 10min contact time, by UV radiation at 35mW.s/cm2 and by ozone at 5mg/L for 10min contact time. Higher doses are required for virus removal by UV and PAA and especially for highly resistant viruses such as F-specific bacteriophage MS2. Ozonation has the advantage of having a strong effect on all types of bacteriophages and protozoa cysts even when low treatment doses and short contact times are applied. The results of this study demonstrated that evaluation of disinfection efficiency of ozone, UV and PAA depends on the criteria and methods employed. Standard method (plate count) results showed an important disinfection effect on culturability, while results from non-standard methods (respiratory activity and β-galactosidase activity assay) indicated less reduction of viable cells. Moreover, the results confirm that disinfectants act on bacteria in different ways. It has been clearly demonstrated that b-galactosidase activity is affected by PAA while UV treatment has no or very limited effect on the enzyme activity. Even without sunlight reactivation, bacterial regrowth in seawater was observed after disinfection of sewage effluents. This study also shows that the biodegradability of sewage effluent for an E coli strain was affected differently by the oxidative disinfectants ozone and PAA. Biodegradability should therefore be considered when evaluating the total disinfection efficiency.

scholarly journals Influence of different sanitizers on food contaminant bacteria: effect of exposure temperature, contact time, and product concentration

2012 ◽  
Vol 32 (2) ◽  
pp. 228-232 ◽  
Author(s):  
Cezar Augusto Beltrame ◽  
Gabriela Busnello Kubiak ◽  
Lindomar Alberto Lerin ◽  
Ieda Rottava ◽  
Altemir José Mossi ◽  
...  
Keyword(s):  
Organic Acids ◽  
Peracetic Acid ◽  
Quaternary Ammonium ◽  
Contact Time ◽  
Industrial Plant ◽  
Meat Industry ◽  
Product Concentration ◽  
E Coli ◽  
Exposure Temperature ◽  
Different Temperatures

The efficiency of four Sanitizers - peracetic acid, chlorhexidine, quaternary ammonium, and organic acids - was tested in this work using different bacteria recognized as a problem to meat industry, Salmonella sp., S. aureus, E. coli and L. monocytogenes. The effects of sanitizer concentration (0.2, 0.5, 0.6, 1.0, 1.1 and 1.4%), at different temperatures (10 and 45 °C) and contact time (2, 10, 15, 18 and 25 minutes) were evaluated. Tests in an industrial plant were also carried out considering previously obtained results. In a general way, peracetic acid presented higher efficiencies using low concentration (0.2%) and contact time (2 minutes) at 10 °C. The tests performed in industrial scale showed that peracetic acid presented a good performance in concentration and contact time lower than that suggested by the suppliers. The use of chlorhexidine and quaternary ammonium led to reasonable results at the indicated conditions, and organic acids were ineffective under concentration and contact time higher than those indicated by the suppliers in relation to Staphylococcus aureus. The results, in general, show that the choice for the most adequate sanitizer depends on the microorganism contaminant, the time available for sanitizer application, and also on the process cost.

Peracetic acid for secondary effluent disinfection: a comprehensive performance assessment

2013 ◽  
Vol 68 (12) ◽  
pp. 2638-2644 ◽  
Author(s):  
M. Antonelli ◽  
A. Turolla ◽  
V. Mezzanotte ◽  
C. Nurizzo
Keyword(s):  
Peracetic Acid ◽  
Vibrio Fischeri ◽  
Pilot Scale ◽  
Selenastrum Capricornutum ◽  
Coliform Bacteria ◽  
Secondary Effluent ◽  
Indicator Organisms ◽  
E Coli ◽  
Comprehensive Performance

The paper is a review of previous research on secondary effluent disinfection by peracetic acid (PAA) integrated with new data about the effect of a preliminary flash-mixing step. The process was studied at bench and pilot scale to assess its performance for discharge in surface water and agricultural reuse (target microorganisms: Escherichia coli and faecal coliform bacteria). The purposes of the research were: (1) determining PAA decay and disinfection kinetics as a function of operating parameters, (2) evaluating PAA suitability as a disinfectant, (3) assessing long-term disinfection efficiency, (4) investigating disinfected effluent biological toxicity on some aquatic indicator organisms (Vibrio fischeri, Daphnia magna and Selenastrum capricornutum), (5) comparing PAA with conventional disinfectants (sodium hypochlorite, UV irradiation). PAA disinfection was capable of complying with Italian regulations on reuse (10 CFU/100 mL for E. coli) and was competitive with benchmarks. No regrowth phenomena were observed, as long as needed for agricultural reuse (29 h after disinfection), even at negligible concentrations of residual disinfectant. The toxic effect of PAA on the aquatic environment was due to the residual disinfectant in the water, rather than to chemical modification of the effluent.

Efficacy of a ceramic siphon household water filter for removal of pathogenic microorganisms: lifespan volume test

2011 ◽  
Vol 1 (2) ◽  
pp. 102-111 ◽  
Author(s):  
Amanda R. Kaufman ◽  
Lisa M. Casanova ◽  
Mark D. Sobsey
Keyword(s):  
Drinking Water ◽  
Feed Water ◽  
Virus Removal ◽  
Water Filter ◽  
E Coli ◽  
Bacteriophage Ms2 ◽  
Point Of Use ◽  
Household Water ◽  
Contaminated Drinking Water ◽  
Pass Through

Treatment of drinking water at the point of use (POU) has demonstrated health benefits for people who have access only to microbially contaminated drinking water. In this work, the ceramic siphon POU water filter was evaluated for its ability to reduce indicator microorganisms in test waters. During batch challenge tests, the filter reduced Escherichia coli in filtered water by 7 log10 (99.999987%) and bacteriophage MS2 by 0.12 log10 (24.0%). Next, a novel continuous flow dosing system allowing sewage-amended feed water to constantly pass through the filters allowed for determination of changes in microbial reductions over time and total volume of water filtered. E. coli B, MS2 and fluorescent microspheres (as a surrogate for Cryptosporidium oocysts) were seeded into test water and dosed to filters at 10, 25 and 50% of the filter's volume lifespan. Microbial removal efficacy decreased as the volume of water filtered increased and test filters did not achieve their volume lifespan before physically failing. The ceramic siphon household water filter is effective in reducing E. coli and surrogates for Cryptosporidium in water, but filter modifications may be needed to achieve acceptable levels of virus removal and to reach the target 7,000 L volume lifespan of the filter.

Virus removal in a pilot-scale ‘advanced’ pond system as indicated by somatic and F-RNA bacteriophages

2005 ◽  
Vol 51 (12) ◽  
pp. 107-110 ◽  
Author(s):  
R.J. Davies-Colley ◽  
R.J. Craggs ◽  
J. Park ◽  
J.P.S. Sukias ◽  
J.W. Nagels ◽  
...  
Keyword(s):  
Pilot Scale ◽  
High Rate ◽  
Virus Removal ◽  
E Coli ◽  
Waste Stabilisation Ponds ◽  
Log Reduction ◽  
Effective Removal ◽  
Solar Uv ◽  
Settling Ponds ◽  
Rna Phage

Advanced pond systems (APS), incorporating high-rate ponds, algal settling ponds, and maturation ponds, typically achieve better and more consistent disinfection as indicated by Escherichia coli than conventional waste stabilisation ponds. To see whether this superior disinfection extends also to enteric viruses, we studied the removal of somatic phages (‘model’ viruses) in a pilot-scale APS treating sewage. Measurements through the three aerobic stages of the APS showed fairly good removal of somatic phage in the summer months (2.2 log reduction), but much less effective removal in winter (0.45 log reduction), whereas E. coli was removed efficiently (>4 logs) in both seasons. A very steep depth-gradient of sunlight inactivation of somatic phage in APS pond waters (confined in silica test tubes) is consistent with inactivation mainly by solar UVB wavelengths. Data for F-RNA phage suggests involvement of longer UV wavelengths. These findings imply that efficiency of virus removal in APS will vary seasonally with variation in solar UV radiation.

A pilot-scale study of peracetic acid and ultraviolet light for wastewater disinfection

2019 ◽  
Vol 5 (8) ◽  
pp. 1453-1463 ◽  
Author(s):  
Abdulrahman H. Hassaballah ◽  
Jeremy Nyitrai ◽  
Christine H. Hart ◽  
Ning Dai ◽  
Lauren M. Sassoubre
Keyword(s):  
Ultraviolet Light ◽  
Peracetic Acid ◽  
Pilot Scale ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
E Coli ◽  
Wastewater Disinfection ◽  
Somatic Coliphage

At the pilot-scale, peracetic acid effectively inactivates fecal coliforms,E. coliandEnterococcusspp. in secondary and tertiary treated wastewater. The addition of UV to PAA treatment increases inactivation of somatic coliphage.

Ozonation of a secondary effluent both for fresh water protection and re-use

2006 ◽  
Vol 1 (2) ◽  
Author(s):  
M. Antonelli ◽  
V. Mezzanotte ◽  
C. Nurizzo
Keyword(s):  
Contact Time ◽  
Pilot Scale ◽  
Secondary Effluent ◽  
Water Protection ◽  
Faecal Coliforms ◽  
Colour Removal ◽  
E Coli ◽  
Log Reduction ◽  
Ionic Compounds ◽  
Higher Doses

49 trials were carried out at pilot scale to evaluate ozonation for polishing a nitrified and filtered effluent discharged in a brook, lying in a recreational protected area whose low and irregular flow provides a negligible dilution. Four ozone doses (3, 5, 7.5 and 10 mg O3/L) were tested and contact time was set at 10 minutes for each of the three contact columns. In most cases, at 3 mg O3/L, residual ozone concentration was below detection limit after 10 minutes contact time. For faecal coliforms and E. coli, log reduction increased from 3 mg O3/L to higher ozone doses, among which no appreciable difference was observed. No improvement in disinfection efficiency was seen for contact times over 10 minutes. Complete disinfection was obtained only in few cases, but final counts complying with the limits for discharge were always met. COD removal was low, while colour removal was significant and clearly increasing from 3 mg O3/L to higher doses. The removal of surfactants slightly increased with increasing dose for non ionic compounds, but not for anionic ones. Data confirmed that slight increases in ozone dose involve the release of bacterial organic matter which partially counterbalance the removal of COD.

scholarly journals Enhanced Production of Carboxymethylcellulase by Recombinant Escherichia coli Strain from Rice Bran with Shifts in Optimal Conditions of Aeration Rate and Agitation Speed on a Pilot-Scale

2019 ◽  
Vol 9 (19) ◽  
pp. 4083
Author(s):  
Chung-Il Park ◽  
Jae-Hong Lee ◽  
Jianhong Li ◽  
Jin-Woo Lee
Keyword(s):  
Escherichia Coli ◽  
Cell Growth ◽  
Pilot Scale ◽  
Aeration Rate ◽  
Coli Strain ◽  
Agitation Speed ◽  
Recombinant Escherichia Coli ◽  
Optimal Conditions ◽  
E Coli ◽  
Enhanced Production

The optimal conditions including the aeration rate and agitation speed of bioreactors for the production of carboxymethylcellulase (CMCase) by a recombinant Escherichia coli KACC 91335P, expressing CMCase gene of B. velezensis A-68, were different from those for its cell growth. The enhanced production of CMCase by E. coli KACC 91335P with the conventional multistage process needs at least two bioreactors. Shifts in the optimal conditions of the aeration rate and agitation speed of the bioreactor from the cell growth of E. coli KACC 91335P to those for its production of CMCase were investigated for development of the simple and economic process with the high productivity and low cost. The production of CMCase by E. coli KACC 91335P with shifts in the optimal conditions of the aeration rate and agitation speed from the cell growth to its production of CMCase in a 100 L pilot-scale bioreactor was 1.36 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for the production of CMCase and it was even 1.54 times higher than that with a fixed optimal conditions of the aeration rate and agitation speed for cell growth. The best time for the shift in the optimal conditions was found to be the mid-log phase of cell growth. Owing to the mixed-growth-associated production of CMCase by E. coli KACC 91335P, shifts in the optimal conditions of the aeration rate and agitation speed of bioreactors from the cell growth to its production of CMCase seemed to result in relatively more cells for the participation in its production of CMCase, which in turn enhanced its production of CMCase. The process with a simple control for shifts in the aeration rate and agitation speed of a bioreactor for the enhanced production of CMCase by E. coli KACC 91335P on the pilot-scale can be directly applied to the industrial-scaled production of cellulase.

Secondary effluent disinfection by peracetic acid (PAA): microrganism inactivation and regrowth, preliminary results

2003 ◽  
Vol 3 (4) ◽  
pp. 269-275 ◽  
Author(s):  
V. Mezzanotte ◽  
M. Antonelli ◽  
A. Azzellino ◽  
S. Citterio ◽  
C. Nurizzo
Keyword(s):  
Flow Cytometry ◽  
Peracetic Acid ◽  
Heterotrophic Bacteria ◽  
Pilot Scale ◽  
Secondary Effluent ◽  
Sample Collection ◽  
Sodium Thiosulphate ◽  
E Coli ◽  
Italian Law ◽  
Bacterial Removal

The paper summarises - in terms of bacterial removal and re-growth - the results of a pilot scale study performed on a continuous flow pilot plant, using peracetic acid (PAA) as disinfecting agent. Trials were carried out with increasing doses and contact times (6, 12, 18, 36, 42, 54 min). The paper reports data deriving from the only doses (15 and 25 mg L-1) which were able to ensure the respect of the limits for agricultural re-use, according to Italian law. Disinfection efficiency was comparable for E. coli, TC and FC, and lower for total heterotrophic bacteria (THB), and clearly grew with increasing Cát values. The effect of contact time was greater at the lower doses. Microbial counts, performed by both traditional methods and flow cytometry, immediately and 6 hours after the sample collection (PAA was previously inactivated by sodium thiosulphate and bovine catalase), showed that no appreciable re-growth took place after 6 hours for coliform group bacteria. THB formed a slightly lower number of colonies immediately after collection than after 6 hours. The slight variations observed after 6 hours were the result of the recovered ability of forming colonies and of the lysis of some of the residual bacteria, as it was shown by flow cytometry.

Impact of Raw Materials and Processing Techniques on The Microbiological Quality of Egyptian Domiati Cheese

2020 ◽  
Keyword(s):  
Raw Materials ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Plate Count ◽  
E Coli ◽  
Fresh Cheese ◽  
Processing Techniques ◽  
The Impact ◽  
Total Yeast

Domiati cheese is the most popular brand of cheese ripened in brine in the Middle East in terms of consumed quantities. This study was performed to investigate the impact of the microbiological quality of the used raw materials, the applied traditional processing techniques and ripening period on the quality and safety of the produced cheese. Three hundred random composite samples were collected from three factories at Fayoum Governorate, Egypt. Collected samples represent twenty-five each of: raw milk, table salt, calf rennet, microbial rennet, water, environmental air, whey, fresh cheese, ripened cheese & swabs from: worker hands; cheese molds and utensils; tanks. All samples were examined microbiologically for Standard Plate Count (SPC), coliforms count, Staphylococcus aureus (S. aureus) count, total yeast & mould count, presence of E. coli, Salmonellae and Listeria monocytogenes (L. monocytogenes). The mean value of SPC, coliforms, S. aureus and total yeast & mould counts ranged from (79×102 CFU/m3 for air to 13×108 CFU/g for fresh cheese), (7×102 MPN/ cm2 for tank swabs to 80×106 MPN/ml for raw milk), (9×102 CFU/g for salt to 69×106 CFU/g for fresh cheese) and (2×102 CFU/cm2 for hand swabs to 60×104 CFU/g for fresh cheese), respectively. Whereas, E. coli, Salmonella and L. monocytogenes failed to be detected in all examined samples. There were significant differences in all determined microbiological parameters (p ≤0.05) between fresh and ripened cheese which may be attributed to different adverse conditions such as water activity, pH, salt content and temperature carried out to improve the quality of the product.

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