scholarly journals Análise de proteínas salivares de corredores de rua após evento esportivo real

2021 ◽  
Vol 10 (10) ◽  
pp. e114101018183
Author(s):  
Cícero Matheus Lima Amaral ◽  
Iago Almeida da Ponte ◽  
Géssica de Souza Martins ◽  
Daniel Freire Lima ◽  
Abelardo Barbosa Moreira Lima Neto ◽  
...  
Keyword(s):  
Acute Exercise ◽  
Submaxillary Gland ◽  
Limited Data ◽  
Acute Effects ◽  
Male Athletes ◽  
Altered Expression ◽  
Cystatin Sn ◽  
Exercise Induced ◽  
Acute Changes ◽  
Induced Changes

The aim was to characterize acute exercise-induced changes in salivary proteins of street runners. Saliva samples from 12 adult male athletes were collected before and immediately after a street race. Two groups were formed based on the distance covered, 5 km (n=4) and 10 km (n=8). Samples were subjected to depletion of amylase, albumin and immunoglobulin G. Then they were concentrated, digested and analyzed by nano-UPLC-tandem nano-ESI-MSE. A total of 69 proteins were identified. Significant changes were observed in the expression of 15 proteins in the 5 km group and 13 proteins in the 10 km group. Among the proteins with altered expression, only 7 had already been described in the literature in similar models (Alpha-Amylase 1, Lactoperoxidase, Alpha Skeletal Muscle Actin, Cystatin-B, Cystatin- SN, Cystatin- SA and Androgen-regulated Protein 3B in the submaxillary gland). This study has shown that street running induces acute changes in the salivary proteome. The results obtained add to the limited data available in the literature in the search for a better understanding of the acute effects of exercise.

Physiological changes in hemostasis associated with acute exercise

1986 ◽  
Vol 60 (3) ◽  
pp. 986-990 ◽  
Author(s):  
M. E. Wheeler ◽  
G. L. Davis ◽  
W. J. Gillespie ◽  
M. M. Bern
Keyword(s):  
Factor Viii ◽  
Acute Exercise ◽  
Density Lipoprotein ◽  
Physiological Changes ◽  
Blood Samples ◽  
Maximum Effort ◽  
Exercise Induced ◽  
Induced Changes ◽  
Treadmill Protocol ◽  
Ristocetin Cofactor

Acute exercise enhances fibrinolytic (FA), factor VIII coagulant and factor VIII ristocetin cofactor activities, and increases the concentration of factor VIII-related antigen. Little is known concerning the mechanisms of these changes. To investigate possible relationships between exercise-induced changes in blood lactate, 2,3-diphosphoglycerate (DPG), and the hemostatic variables, a branching multistage treadmill protocol was used to exercise male volunteers to a maximum effort. Blood samples were drawn before, immediately post-, and 8 min postexercise. All hemostatic variables were significantly (P less than 0.05) increased postexercise. Highest values for factor VIII coagulant, factor VIII-related antigens and factor VIII ristocetin cofactor were observed at 8 min postexercise. Significant (P less than 0.001) correlations were found postexercise for lactate with factor VIII coagulant (r = 0.64), while no association between pre-, post-, or 8 min postexercise. Postexercise lactate demonstrated a significant correlation (r = +0.81), which was strengthened by including the preexercise high-density lipoprotein (HDL) concentrations (r = +0.87). Consequently, the expected postexercise FA may be calculated from the observed values for postexercise lactate and preexercise HDL. The correlations of lactate with postexercise FA and with postexercise factor VIII coagulant may reflect a common stimulus for these exercise-induced changes.

A novel, noninvasive transdermal fluid sampling methodology: IGF-I measurement following exercise

2011 ◽  
Vol 300 (6) ◽  
pp. R1326-R1332 ◽  
Author(s):  
D. E. Scofield ◽  
H. L. McClung ◽  
J. P. McClung ◽  
W. J. Kraemer ◽  
K. R. Rarick ◽  
...  
Keyword(s):  
Acute Exercise ◽  
Active Muscle ◽  
Acute Bout ◽  
Factor I ◽  
Exercise Condition ◽  
Plyometric Exercise ◽  
Igf I ◽  
Exercise Induced ◽  
Induced Changes ◽  
Fluid Sampling

This study tested the hypothesis that transdermal fluid (TDF) provides a more sensitive and accurate measure of exercise-induced increases in insulin-like growth factor-I (IGF-I) than serum, and that these increases are detectable proximal, but not distal, to the exercising muscle. A novel, noninvasive methodology was used to collect TDF, followed by sampling of total IGF-I (tIGF-I) and free IGF-I (fIGF-I) in TDF and serum following an acute bout of exercise. Experiment 1: eight men (23 ± 3 yrs, 79 ± 7 kg) underwent two conditions (resting and 60 min of cycling exercise at 60% V̇o2peak) in which serum and forearm TDF were collected for comparison. There were no significant changes in tIGF-I or fIGF-I in TDF obtained from the forearm or from serum following exercise ( P > 0.05); however, the proportion of fIGF-I to tIGF-I in TDF was approximately fourfold greater than that of serum ( P ≤ 0.05). These data suggest that changes in TDF IGF-I are not evident when TDF is sampled distal from the working tissue. To determine whether exercise-induced increases in local IGF-I could be detected when TDF was sampled directly over the active muscle group, we performed a second experiment. Experiment 2: fourteen subjects (22 ± 4 yr, 68 ± 11 kg) underwent an acute plyometric exercise condition consisting of 10 sets of 10 plyometric jumps with 2-min rest between sets. We observed a significant increase in TDF tIGF-I following exercise ( P ≤ 0.05) but no change in serum tIGF-I ( P > 0.05). Overall, these data suggest that TDF may provide a noninvasive means of monitoring acute exercise-induced changes in local IGF-I when sampled in proximity to exercising muscles. Moreover, our finding that the proportion of free to tIGF-I was greater in TDF than in serum suggests that changes in local IGF-I may be captured more readily using this system.

scholarly journals Acute exercise-induced changes in learning-related biomarkers and word learning success

2021 ◽  
Vol 24 ◽  
pp. S55
Author(s):  
J. Coombes ◽  
D. Copland ◽  
K. Erickson ◽  
K. McMahon ◽  
M.-P. McSween ◽  
...  
Keyword(s):  
Word Learning ◽  
Acute Exercise ◽  
Learning Success ◽  
Exercise Induced ◽  
Induced Changes

Acute Exercise Induced Changes of Gene-Specific DNA Methylation in Natural Killer Cells

2017 ◽  
Vol 49 (5S) ◽  
pp. 88
Author(s):  
Christina Koliamitra ◽  
Wilhelm Bloch ◽  
Alexander Schenk ◽  
Walter Pulverer ◽  
Philipp Zimmer
Keyword(s):  
Dna Methylation ◽  
Natural Killer Cells ◽  
Natural Killer ◽  
Acute Exercise ◽  
Killer Cells ◽  
Exercise Induced ◽  
Induced Changes

scholarly journals Effect of glutamine supplementation on exercise-induced changes in lymphocyte function

2001 ◽  
Vol 281 (4) ◽  
pp. C1259-C1265 ◽  
Author(s):  
Karen Krzywkowski ◽  
Emil Wolsk Petersen ◽  
Kenneth Ostrowski ◽  
Jens Halkjær Kristensen ◽  
Julio Boza ◽  
...  
Keyword(s):  
T Cells ◽  
Killer Cell ◽  
Glutamine Supplementation ◽  
Lymphocyte Function ◽  
Double Blind ◽  
Male Athletes ◽  
Exercise Induced ◽  
Induced Changes ◽  
Immune Changes ◽  
Response To Exercise

The purpose of this study was to investigate the possible role of glutamine in exercise-induced impairment of lymphocyte function. Ten male athletes participated in a randomized, placebo-controlled, double-blind crossover study. Each athlete performed bicycle exercise for 2 h at 75% of maximum O2consumption on 2 separate days. Glutamine or placebo supplements were given orally during and up to 2 h postexercise. The trial induced postexercise neutrocytosis that lasted at least 2 h. The total lymphocyte count increased by the end of exercise due to increase of both CD3+TCRαβ+and CD3+TCRγδ+T cells as well as CD3−CD16+CD56+natural killer (NK) cells. Concentrations of CD8+and CD4+T cells lacking CD28 and CD95 on their surface increased more than those of cells expressing these receptors. Within the CD4+cells, only CD45RA−memory cells, but not CD45RA+naive cells, increased in response to exercise. Most lymphocyte subpopulations decreased 2 h after exercise. Glutamine supplementation abolished the postexercise decline in plasma glutamine concentration but had no effect on lymphocyte trafficking, NK and lymphokine-activated killer cell activities, T cell proliferation, catecholamines, growth hormone, insulin, or glucose. Neutrocytosis was less pronounced in the glutamine-supplemented group, but it is unlikely that this finding is of any clinical significance. This study does not support the idea that glutamine plays a mechanistic role in exercise-induced immune changes.

scholarly journals Effects of Exercise Training on Neurotrophic Factors and Subsequent Neuroprotection in Persons with Multiple Sclerosis—A Systematic Review and Meta-Analysis

2021 ◽  
Vol 11 (11) ◽  
pp. 1499
Author(s):  
Mette D. Diechmann ◽  
Evan Campbell ◽  
Elaine Coulter ◽  
Lorna Paul ◽  
Ulrik Dalgas ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Neurotrophic Factors ◽  
Neurotrophic Factor ◽  
Meta Analysis ◽  
Group Differences ◽  
Exercise Interventions ◽  
Exercise Induced ◽  
Acute Changes ◽  
Meta Analyses ◽  
Induced Changes

Background: Evidence indicates that exercise holds the potential to counteract neurodegeneration experienced by persons with multiple sclerosis (pwMS), which is in part believed to be mediated through increases in neurotrophic factors. There is a need to summarize the existing evidence on exercise-induced effects on neurotrophic factors alongside neuroprotection in pwMS. Aim: To (1) systematically review the evidence on acute (one session) and/or chronic (several sessions) exercise-induced changes in neurotrophic factors in pwMS and (2) investigate the potential translational link between exercise-induced changes in neurotrophic factors and neuroprotection. Methods: Five databases (Medline, Scopus, Web of Science, Embase, Sport Discus) were searched for randomized controlled trials (RCT) examining the effects of exercise (all modalities included) on neurotrophic factors as well as measures of neuroprotection if reported. The quality of the study designs and the exercise interventions were assessed by use of the validated tool TESTEX. Results: From N = 337 identified studies, N = 14 RCTs were included. While only N = 2 of the identified studies reported on the acute changes in neurotrophic factors, all N = 14 RCTs reported on the chronic effects, with N = 9 studies revealing between-group differences in favor of exercise. This was most prominent for brain-derived neurotrophic factor (BDNF), with between-group differences in favor of exercise being observed in N = 6 out of N = 12 studies. Meta-analyses were applicable for three out of 10 different identified neurotrophic factors and revealed that exercise can improve the chronic levels of BDNF (delta changes; N = 9, ES = 0.78 (0.27; 1.28), p = 0.003, heterogeneity between studies) and potentially also ciliary neurotrophic factor (CNTF) (N = 3, ES = 0.24 (−0.07; 0.54), p = 0.13, no heterogeneity between studies) but not nerve growth factor (NGF) (N = 4, ES = 0.28 (−0.55; 1.11), p = 0.51, heterogeneity between studies). Indicators of neuroprotection (e.g., with direct measures of brain structure assessed by MRI) were assessed in N = 3 of the identified studies only, with N = 2 partly supporting and thus indicating a potential translational link between increases in neurotrophic factors and neuroprotection. Conclusion: The present study reveals that exercise can elicit improvements in chronic levels of BDNF in pwMS, whereas the effects of exercise on chronic levels of other neurotrophic factors and on acute levels of neurotrophic factors in general, along with a potential translational link (i.e., with exercise-induced improvements in neurotropic factors being associated with or even mediating neuroprotection), are sparse and inconclusive. There is a need for more high-quality studies that assess neurotrophic factors (applying comparable methods of blood handling and analysis) concomitantly with neuroprotective outcome measures. Review Registration: PROSPERO (ID: CRD42020177353).

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