scholarly journals Hygienic-sanitary quality of ready-to-eat salmon sashimi (Salmo Salar)

2021 ◽  
Vol 10 (12) ◽  
pp. e573101220900
Author(s):  
Iara Oliveira Arruda ◽  
Talitha Maria Porfírio ◽  
Edgar Nascimento ◽  
Demétrio de Abreu Sousa ◽  
Daniel Oster Ritter ◽  
...  
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Antimicrobial Agents ◽  
Heterotrophic Bacteria ◽  
International Standards ◽  
Thermotolerant Coliforms ◽  
Safe Limits ◽  
Resistance To Penicillin ◽  
Coagulase Positive Staphylococci

Fifty sashimi samples from 5 restaurants were characterized for enumeration of Aerobic Psychrotrophic Heterotrophic Bacteria (APHB) and Aerobic Mesophilic Heterotrophic Bacteria (AMHB), Enterobacteriaceae, coliforms at 45ºC (thermotolerant coliforms), coagulase-positive Staphylococci, presence of Vibrio parahaemolyticus and Salmonella sp., and determination of hydrogen potential (pH) and total volatile basic nitrogen (TVB-N). The bacterial isolates were evaluated for their resistance profile to the antimicrobial agents Penicillin, Ampicillin, Cefoxitin, Cefotaxime, Amikacin, Gentamicin, Tetracycline, and Trimethropim-sulfamethoxazole. The results showed that APHB, AMHB and coagulase-positive Staphylococci counts and the determination of pH and TVB-N were in accordance with national and international standards adopted as safe limits for consumption. In contrast, the Enterobacteriaceae and thermotolerant coliforms counts and presence of Salmonella sp. and V. parahaemolyticus were in disagreement with those standards, raising concern about the hygienic-sanitary quality of sashimi. The Staphylococcus aureus and V. parahaemolyticus isolates showed resistance to Penicillin, Ampicillin, Cefoxitin, Cefotaxime, Tetracycline, Gentamicin, and Amikacin, while the Salmonella sp. isolate showed no resistance to all the antimicrobial agents studied. The results showed that 48% of the samples were fit for consumption while 52% had unsatisfactory hygienic-sanitary quality for the parameters evaluated.

scholarly journals Making progress in determining quality: positional accuracy of the new 1:25,000 scale digital cartography

2021 ◽  
Vol 4 ◽  
pp. 1-5
Author(s):  
Bárbara Cubillos ◽  
Ángela Ortíz ◽  
Germán Aguilera ◽  
Sergio Rozas ◽  
Claudio Reyes ◽  
...  
Keyword(s):  
Data Model ◽  
Early Stage ◽  
International Standards ◽  
Positional Accuracy ◽  
Intelligence Agency ◽  
Digital Cartography ◽  
Data Store ◽  
The Military

Abstract. The digital cartographic coverage at 1:25,000 that the Military Geographic Institute is creating has been worked on using international standards, so that it constitutes a standardized and interoperable tool, for the various areas of activity in Chile. In this context, the ISO TC 211 standards and the TDS (Topographic Data Store) data model developed by the National Geospatial-Intelligence Agency (NGA) are being used.Apart from using these standards, efforts have been aimed, from an early stage, at the determination of the quality of this product, starting this process with the study for a methodology to measure Positional Accuracy. The method defined conforms to the NSSDA test; for this, points measured in the terrain especially for this control are used, also the elimination of points that are out of range under the Chauvenet Criteria. Finally, the positional accuracy is declared in the metadata.

Incidence of Vibrio parahaemolyticus in and the Microbiological Quality of Seafood in North Carolina1

1980 ◽  
Vol 43 (10) ◽  
pp. 769-773 ◽  
Author(s):  
C. R. HACKNEY ◽  
B. RAY ◽  
M. L. SPECK
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
The United States ◽  
Statistical Correlation ◽  
Fecal Coliforms ◽  
Processing Plants ◽  
United States Food ◽  
Coagulase Positive Staphylococci

Microbiological analyses of 716 seafood samples over a 3-year period revealed that the microbiological quality of fresh seafood in North Carolina was generally acceptable. The mean aerobic counts (APC) and fecal coliform counts were low as was the occurrence of enteric pathogens, except for Vibrio parahaemolyticus and coagulase-positive staphylococci. Salmonella species were isolated from three samples, but the fecal coliform counts of these samples far exceeded the shellfish standard of the United States Food and Drug Administration. Coagulase-positive staphylococci were isolated in low numbers from nearly all the different types of seafoods; unpasteurized crabmeat and head-peeled shrimp samples showed the highest counts (10% of these samples had numbers which exceeded 100/g). V. parahaemolyticus occurred frequently in fresh seafood (overall 46% of the samples were positive) and its numbers showed a definite seasonal variation. No positive statistical correlation was found between the numbers of V. parahaemolyticus and the bacteriological indices, such as coliforms, fecal coliforms, enterococci and APC. Processing practices were found to influence the occurrence of V. parahaemolvticus in seafood; for example, improperly cleaned flumes were found to be a reservoir for V. parahaemolyticus in mechanical scallop processing plants. Also ‘picking’ waste ‘containers’ were found to be sources of V. parahaemolyticus in crab processing plants. Some processing practices such as heat shocking of oysters to facilitate opening were found to reduce the numbers of V. parahaemolyticus. Fifty V. parahaemolyticus isolates from different seafoods were tested for their Kanagawa reaction and all were found to be negative.

scholarly journals Microbiological and Marine Biotoxins Contamination of Raw Bivalve Molluscs Commercially Available in Poland

2012 ◽  
Vol 56 (4) ◽  
pp. 563-568 ◽  
Author(s):  
Remigiusz Pomykała ◽  
Mirosław Michalski ◽  
Artur Jóźwik ◽  
Jacek Osek
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Paralytic Shellfish Poisoning ◽  
Shellfish Poisoning ◽  
Bivalve Molluscs ◽  
E Coli ◽  
Marine Biotoxins ◽  
Amnesic Shellfish Poisoning ◽  
Paralytic Shellfish ◽  
Coagulase Positive Staphylococci

Abstract The study was carried out on live bivalve molluscs available on Polish market. Microbiological tests were performed for the presence of Salmonella sp., Vibrio parahaemolyticus, spore-forming anaerobe bacteria, and coagulase-positive Staphylococcus sp., and for the enumeration of Escherichia coli. ELISA was used for the determination of marine biotoxins, paralytic shellfish poisoning (PSP), amnesic shellfish poisoning (ASP), and diarrhoeic shellfish poisoning (DSP). Microbiological examinations were performed according to ISO and Polish Standards. Salmonella sp. was not detected in any sample tested. Coagulase-positive staphylococci were identified in 9.0% of the samples. V. parahaemolyticus was isolated from 17.0% of mussels. Shellfish were highly contaminated by anaerobes, which were isolated from 68.0% of the samples. The number of E. coli ranged from <2.0 x 101 up to >1.8 x 104 MPN/100 g. The majority of mussels were free from the marine biotoxins tested or contained them bellow the permitted level. The analysis of microbiological and toxicological status of raw bivalve molluscs available on Polish market indicates that they are generally safe for the consumers.

scholarly journals Microbiology of Charcoal-Broiled European River Lampreys (Lampetra fluviatilis) Stored at 3 and 22° C

2003 ◽  
Vol 66 (12) ◽  
pp. 2332-2335 ◽  
Author(s):  
LAURI O. MERIVIRTA ◽  
K. JOHANNA BJÖRKROTH ◽  
HANNU J. KORKEALA
Keyword(s):  
Hazard Analysis ◽  
Microbiological Quality ◽  
Critical Control Points ◽  
Two Samples ◽  
Processing Plants ◽  
Plate Counts ◽  
Thermotolerant Coliforms ◽  
The Mean

The microbiological quality of 30 production lots of charcoal-broiled river lampreys was studied at three lamprey processing plants (plants A, B, and C). Samples were taken directly after charcoal broiling and stored at 22 and 3°C. Lampreys were examined on the day of manufacture, and those kept at 22°C were examined every second day for 6 days. Samples kept at 3°C were examined every fourth day for up to 24 days. On the production day, the mean aerobic plate counts (APCs) for broiled lampreys from plants A, B, and C were 2.29 log CFU/g, 1.88 log CFU/g, and undetectable (1.67 log CFU/g), respectively. At 22°C, the mean APCs for samples from plants A, B, and C increased markedly within 4 days, and after 6 days the counts for samples from these plants were 8.56, 5.04, and 6.23 log CFU/g, respectively. Chilling and storage at 3°C remarkably improved the shelf life of the product. The levels of bacteria in charcoal-broiled river lampreys from plant A were higher than those in lampreys from plants B and C. No significant increases in APCs were observed during storage at 3°C for 24 days; mean APCs did not exceed 2.80 log CFU/g for samples from any plant. Staphylococcus aureus was found in two samples. No lactic acid bacteria, thermotolerant coliforms, enterococci, Clostridium perfringens, or Listeria monocytogenes was detected. Microbiological data from this study will be used for the development of a hazard analysis for the determination of critical control points.

scholarly journals Assessment of the sanitary quality of ready to eat sesame, a low moisture street food from Burkina Faso

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Muller K. A. Compaoré ◽  
Bazoin Sylvain Raoul Bazie ◽  
Marguerite E. M. Nikiema ◽  
Virginie Marie Dakené ◽  
René Dembélé ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Sesame Seed ◽  
International Standards ◽  
Microbial Quality ◽  
Ambient Conditions ◽  
E Coli ◽  
Wide Range ◽  
Thermotolerant Coliforms ◽  
Microbial Groups

Abstract Background Microbial contamination of edible low moisture food poses a significant public health risk for human. In this study, the microbial quality of sweet dehulled sesame seed croquettes, salted dehulled sesame seed and the raw sesame seed, sold under ambient conditions were examined. The samples were collected in the cities of Burkina Faso. The first type is sweet dehulled sesame seed croquettes (n1 = 25); the second type is salted dehulled sesame seed (n2 = 25) and the third type is raw sesame seed (n3 = 25). Assessment of the microbial quality was based on the total aerobic mesophilic bacteria, the thermotolerant coliforms, the yeasts and moulds, the E. coli, and the Salmonella spp. using ISO methods. Results The results showed the presence of microorganisms varying from <1.0 to 1.72 × 105 CFU g− 1 for thermotolerant coliforms, from <1.0 to 6,12 × 106 CFU g− 1 for the total mesophilic aerobic flora and from <1.0 to 8.10 × 105 CFU g− 1 for yeasts and moulds. The higher contaminations rates were mostly observed in raw sesame seed samples. No E coli or Salmonella pathogens were detected. Based on international standards of dehydrated food, 50.67% of the ready to eat sesame are satisficing while 17.33% are acceptable and 32% are not satisficing. Conclusion Attention should be emphasized on the processing practices, especially in crowded places where RTE sesames seeds are mostly sold. The high numbers of all microbial groups in these sesame seed samples suggested that the production of RTE sesame seed should be improved by better hygiene. This study highlights also that RTE sesame seed might harbor a wide range of microorganisms when processes are weak of hygiene.

scholarly journals Determination of staphylococcal enterotoxins in cheese by immunoenzyme assays

2012 ◽  
Vol 64 (4) ◽  
pp. 1449-1454 ◽  
Author(s):  
Vesna Jankovic ◽  
Vesna Djordjevic ◽  
Brankica Lakicevic ◽  
Branka Borovic ◽  
B. Velebit ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Food Poisoning ◽  
Raw Milk ◽  
Foodborne Illness ◽  
Staphylococcal Enterotoxins ◽  
Official Gazette ◽  
Microbiological Criteria ◽  
Coagulase Positive Staphylococci

Staphylococcal food poisoning is one of the most common foodborne diseases resulting from the ingestion of staphylococcal enterotoxins (SEs) preformed in foods by enterotoxigenic strains of coagulase-positive staphylococci (CPS), mainly Staphylococcus aureus. The presence of enterotoxigenic strains of coagulase-positive staphylococci in raw milk during the production process leads to the contamination of products and outbreaks of alimentary intoxication. The problem of Staphylococcus aureus in cheese remains significant on a global level. Domestic cheese contaminated with enterotoxigenic staphylococci can result in the formation of enterotoxin, which can produce foodborne illness when the product is ingested. Due to microbiological contamination, microbiological criteria are tools that can be used in assessing the safety and quality of foods. In order to avoid foodborne illness, the Serbian Regulation on General and Special Conditions for Food Hygiene (Official Gazette of RS, No. 72/10) provides microbiological criteria for staphylococcal enterotoxins in dairy products.

scholarly journals Microbiological quality assessment of five common foods sold at different points of sale in Burkina-Faso

2021 ◽  
Author(s):  
Muller Kiswendsida Abdou COMPAORE ◽  
Stéphane Dissinviel KPODA ◽  
Raoul Bazoin Sylvain BAZIE ◽  
Marcelline OUEDRAOGO ◽  
Alphonse YAKORO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Burkina Faso ◽  
Microbiological Quality ◽  
Microbial Quality ◽  
Food Hygiene ◽  
Different Types ◽  
Thermotolerant Coliforms ◽  
Coagulase Positive Staphylococci

The aim of the present study was to assess the microbial quality of five different types of food such as bread, pasta, rice with sauce, beans and milk sold in five localities of Burkina Faso. One hundred and one (101) samples were collected and microbial quality were assessed by evaluating the food hygiene indicators such as total aerobic mesophilic flora, total coliforms, thermotolerant coliforms, yeast and mould. Food safety indicators such as Escherichia coli , Salmonella , coagulase-positive staphylococci, Clostridium perfringens and Bacillus cereus were checked too. All samples were analyzed under ISO methods.

scholarly journals AMR - An R Package for Working with Antimicrobial Resistance Data

2019 ◽  
Author(s):  
Matthijs S. Berends ◽  
Christian F. Luz ◽  
Alexander W. Friedrich ◽  
Bhanu N.M. Sinha ◽  
Casper J. Albers ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Antimicrobial Agents ◽  
Reference Data ◽  
Data Cleaning ◽  
Laboratory Data ◽  
R Package ◽  
International Standards ◽  
World Health ◽  
Resistance Data

AbstractAntimicrobial resistance is an increasing threat to global health. Evidence for this trend is generated in microbiological laboratories through testing microorganisms for resistance against antimicrobial agents. International standards and guidelines are in place for this process as well as for reporting data on (inter-)national levels. However, there is a gap in the availability of standardized and reproducible tools for working with laboratory data to produce the required reports. It is known that extensive efforts in data cleaning and validation are required when working with data from laboratory information systems. Furthermore, the global spread and relevance of antimicrobial resistance demands to incorporate international reference data in the analysis process.In this paper, we introduce the AMR package for R that aims at closing this gap by providing tools to simplify antimicrobial resistance data cleaning and analysis, while incorporating international guidelines and scientifically reliable reference data. The AMR package enables standardized and reproducible antimicrobial resistance analyses, including the application of evidence-based rules, determination of first isolates, translation of various codes for microorganisms and antimicrobial agents, determination of (multi-drug) resistant microorganisms, and calculation of antimicrobial resistance, prevalence and future trends. The AMR package works independently of any laboratory information system and provides several functions to integrate into international workflows (e.g. WHONET software provided by the World Health Organization).

scholarly journals Determination of thermotolerant coliforms present in coconut water produced and bottled in the Northeast of Brazil

2017 ◽  
Vol 21 (0) ◽  
Author(s):  
Vandbergue Santos Pereira ◽  
Johnatan Wellisson da Silva Mendes ◽  
Lorena Alves Oliveira ◽  
Carlos Eberton Alves Mangueira ◽  
Edlânia Moraes Rodrigues ◽  
...  
Keyword(s):  
Microbiological Quality ◽  
Coconut Water ◽  
Automated System ◽  
Most Probable Number ◽  
Probable Number ◽  
Providencia Alcalifaciens ◽  
Thermotolerant Coliforms ◽  
Preventive Methods

Abstract Coconut water is considered to be a natural isotonic drink and its marketing is gradually increasing. The objective of the present study was to evaluate the microbiological quality of the coconut water produced and bottled in the Northeast of Brazil. Products form ten industries from different states in the Northeast of Brazil were analyzed. The most probable number (MPN) method was used to quantify the coliforms. Samples showing positive for coliforms were seeded on ChromAgar Orient plates and the bacteria identified from isolated colonies using the automated system Vitek 2 (BioMérieux), according to the manufacturer's instructions for the preparation of the inoculum, incubation, reading and interpretation. The samples showed thermotolerant coliform counts between 6.0×102 and 2.6×104 MPN/100 mL. The presence of Klebsiella pneumoniae, Morganella morganii and Providencia alcalifaciens was observed. The implementation of preventive methods and monitoring of the water quality by the industries is required.

scholarly journals Influence of different sources of contamination on the microbiological quality of lettuce in the Teresópolis region, RJ, Brazil

2020 ◽  
Vol 25 (2) ◽  
pp. 229-235
Author(s):  
Monica Conceição Nunes-Carvalho ◽  
Marcelly Miranda Aybal Jayme ◽  
Flávia Lima do Carmo ◽  
Alexandre dos Santos Pyrrho ◽  
Selma Gomes Ferreira Leite ◽  
...  
Keyword(s):  
Irrigation Water ◽  
Heterotrophic Bacteria ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Metropolitan Region ◽  
Cultivated Soil ◽  
Thermotolerant Coliforms ◽  
Different Sources ◽  
Sources Of Contamination

ABSTRACT Irrigation water and cultivated soil have been identified as possible sources of contamination in several crops. In certain vegetables that are eaten raw, such as lettuce, this contamination can lead to public health problems. Aiming to evaluate the influence of these sources on the quality of lettuce grown in the Córrego Sujo Basin, Teresópolis, RJ, an important agricultural pole whose production services the metropolitan region of Rio de Janeiro, water from different sources (spring, weir and river) was collected in this region, as well as samples of soil and lettuce irrigated with these waters, to carry out conventional microbiological analyzes (counts of total heterotrophic bacteria and thermotolerant coliforms) and molecular analyzes (PCR-DGGE). The count of fecal coliforms in lettuce suggests that there is an influence of irrigation water and the cultivated soil on the contamination of these vegetables. The grouping of bacterial communities in the different samples obtained by the PCR-DGGE technique shows that irrigation water has a greater influence on the contamination of these vegetables in relation to the soil where they are grown. These results corroborate the need to monitor water bodies used for irrigation and demonstrate that the PCR-DGGE technique is of great value for the study of microbial communities and, when associated with specific primers, can help in the detection of pathogens in food.

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