scholarly journals Panorama mundial das tecnologias referentes ao uso do ejiao por meio de busca em base de patentes

2021 ◽  
Vol 10 (2) ◽  
pp. e25210212428
Author(s):  
Lucas Santana da Fonseca ◽  
Claudia Beatriz Lopes Almeida ◽  
Tobyas Maia Albuquerque Mariz ◽  
Chiara Albano Araújo Oliveira ◽  
Pierre Barnabe Escodro
Keyword(s):  
Colla Corii Asini

O colla corii asini ou donkey-hide gelatin ou ejiao é uma gelatina obtida do colágeno da pele de jumentos, utilizado há milhares de anos com diversas finalidades na medicina tradicional chinesa. A demanda pelo ejiao, misturado a ervas e outros ingredientes da medicina chinesa, aumentou devido a ascensão econômica da população chinesa em geral. As peles de jumentos oriundas de países como o Quirguistão, o Brasil e o México seguem para a China para a produção do Ejiao. Nessa cadeia extrativista a África é o território mais afetado, tanto no número de animais abatidos quanto no impacto social e econômico. Este trabalho é um levantamento prospectivo de patentes sobre inovações e descobertas do uso terapêutico do ejiao. A pesquisa foi realizada em três bases de patentes e a partir destas foram recuperados o total de 1837 documentos sobre tecnologias que fazem uso do ejiao. Analisando a trajetória temporal das publicações na área, observa-se que as invenções tiveram início na década de 90 e atingiram seu ápice no ano de 2015, contudo observou-se uma recaída nos anos seguintes, a suposição é de que o declínio tenha sido ocasionado pela redução populacional dos asininos na China e nos países fornecedores de outras regiões do mundo, além não existência da cadeia produtiva de pele de jumentos.

Analysis of Heparan sulfate/heparin from Colla corii asini by liquid chromatography-electrospray ion trap mass spectrometry

2019 ◽  
Vol 36 (3) ◽  
pp. 211-218 ◽  
Author(s):  
Jiayan Du ◽  
Su Liu ◽  
Quntao Liang ◽  
Jianghui Lin ◽  
Lilong Jiang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Heparan Sulfate ◽  
Ion Trap ◽  
Ion Trap Mass Spectrometry ◽  
Colla Corii Asini

Quantitative detection of donkey hide gelatin (colla corii asini) adulteration by real-time PCR based on single-copy nuclear genes

2020 ◽  
Author(s):  
Rui Liu ◽  
Luyao Zhao ◽  
Zhiying Wang ◽  
Tong Li ◽  
Ailiang Chen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Single Copy ◽  
High Price ◽  
Quantitative Detection ◽  
Potential Candidate ◽  
Animal Products ◽  
Medicinal Value ◽  
System Establishment ◽  
Colla Corii Asini

Donkey hide gelatin (colla corii asini) is highly recognized for its high nutritional value, especially the medicinal value. However, it is also a potential candidate for adulteration because of its low yield and high price. In order to quantitatively detect adulterated donkey-hide gelatin with all possible adulterated animal species, a real-time PCR approach based on single-copy housekeeping nuclear reference primers was proposed in this study. For the system establishment, mixtures containing designated contents of pig hide with donkey-hide were employed to generate a calibration curve based on the ratio of Ct (Specificity/Reference) with reasonable linearity (5%-100%). Then, a set of experiments were performed on commercially available samples. The proposed PCR approach could specifically identify donkey-hide from mixed animal products and quantify the content of donkey-hide gelatin, thus facilitating the control over this novel form of donkey hide gelatin adulteration.

scholarly journals Improving antioxidant and antiproliferative activities of colla corii asini hydrolysates using ginkgo biloba extracts

2018 ◽  
Vol 6 (4) ◽  
pp. 765-772 ◽  
Author(s):  
Xiaobing Xu ◽  
Shangwei Guo ◽  
Xianghui Hao ◽  
Hui Ma ◽  
Yanping Bai ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
Ginkgo Biloba Extracts ◽  
Colla Corii Asini ◽  
Antiproliferative Activities

scholarly journals Chemical constituents and bioactivities of Colla corii asini

2014 ◽  
Vol 8 (5) ◽  
pp. 201-207 ◽  
Author(s):  
Dongliang Wang ◽  
Wenwen Ru ◽  
Yunpeng Xu ◽  
Jianling Zhang ◽  
Xianxian He ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Colla Corii Asini

Extraction of Trace Amount of Severely Degraded DNA

2009 ◽  
Vol 64 (7-8) ◽  
pp. 581-589 ◽  
Author(s):  
Pin Lv ◽  
Xiangshan Zhou ◽  
Jinhua You ◽  
Bang-Ce Ye ◽  
Yuanxing Zhang
Keyword(s):  
Proteinase K ◽  
Degraded Dna ◽  
Dna Fragments ◽  
Dna Yield ◽  
Equus Asinus ◽  
Spin Column ◽  
Purification System ◽  
Dna Fragment ◽  
Yield And Purity ◽  
Colla Corii Asini

DNA extraction from food is always problematic especially from highly processed samples which contain only trace amounts of severely degraded DNA fragments. In this work, to extract trace amounts of small DNA fragments of the traditional Chinese medicine (TCM) colla corii asini derived from highly processed Equus asinus skin, three strategies were compared for its authentication. With some optimizations, the modified QIAquick spin column method achieved higher DNA yield and purity in comparison with the “SDS/proteinase K” method and the “Wizard magnetic DNA purification system for food” method. Further studies showed that at least 0.4 g colla corii asini was needed to obtain enough DNA extracts for PCR-based detection by the method and only amplicons of less than 100 bp could be generated from the DNA extracts which confirmed the efficiency of the method in small DNA fragment extraction. The DNA obtained by this method was suitable to be used in PCR-based authentications.

Hematopoietic Effect of Fractions from the Enzyme-Digested colla corii asini on Mice with 5-Fluorouracil Induced Anemia

2007 ◽  
Vol 35 (05) ◽  
pp. 853-866 ◽  
Author(s):  
Hongzhong Wu ◽  
Fan Yang ◽  
Shuya Cui ◽  
Yufeng Qin ◽  
Jianwen Liu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Transforming Growth Factor ◽  
Cell Activity ◽  
Erythroid Cell ◽  
Gm Csf ◽  
Cell Counts ◽  
Plasma Cytokine Level ◽  
Immature Granulocyte ◽  
Specific Peptide ◽  
Colla Corii Asini

Effects of fractions A and B from enzyme-digested traditional Chinese medicine colla corii asini on mice with 5-fluorouracil-induced anemia were investigated. The purpose of this study was to further understand the hematopoietic activities and mechanisms of colla corii asini. The fractions A and B were administered to anemic mice for 12 days. After confirming the anti-anemic effect of fractions A and B, we examined the effects of fractions A and B on immature granulocyte and erythroid cell activity and plasma cytokine level. Fraction A administration at 2 g/kg and 1 g/kg and fraction B administration at 1.6 g/kg and 0.8 g/kg activated granulocyte and erythrocyte progenitor cells in bone marrow and erythrocyte progenitors in spleen, led to the recovery of white blood cell and red blood cell counts, and increased the percentage of peripheral reticulocytes in red cells. The GM-CSF and EPO production determined by examining GM-CSF mRNA and EPO mRNA in the kidney and liver of the anemic mice were also enhanced. This treatment significantly increased serum GM-CSF and EPO level and lowered serum transforming growth factor ( TGF -β) level. These results suggested that fractions A and B promoted hematopoiesis by activating immature granulocyte and erythroid cells, partly by stimulating GM-CSF and EPO secretion and suppressing TGF -β release. Identification of a specific peptide or protein is still required for the development of a novel medicine for anemia caused by malignancy or chemotherapy.

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